FastQCFastQC Report
Sat 31 Dec 2022
SRR5848218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70345
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGCCGTCT7061.0036249911152177No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGC6630.9424976899566423No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTTATACA2080.29568554979031914No Hit
TTCATAGGGATTCTGGTTTTCAACCAAGTATCTATGCTTTTCTGCTCAAACT1190.1691662520434999No Hit
TAGAAGGGGACCCACACCTTTTTTTCGTAGGTGGGCTTTTCCTATCAGAGCT1190.1691662520434999No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTT1180.16774468690027722No Hit
ATCTTAGGGGAAGACAACCTTGATTTCAGTGTGAATTCAGAGGTGTCTGTGT1150.16347999147060915No Hit
TTTTGGGGGAAATGGAATTGGGCATGAAAAATGTGAACGGCACAAGGCAAAT1090.154950600611273No Hit
ACTTTCGGGAGATGTTCAAATTTCAGATCTTACCATCATCCAACTTAAACTG1050.14926434003838226No Hit
TGCTACGGGTGAGGGTGAAATTAAGGGAAGGCAAAGTCCAGGCACCAGAGTG1050.14926434003838226No Hit
ACATTTGGGCCGTCTGAGGAGCAGTGAGGTCTTCAAGTGGCTCATCCCTGAC1040.14784277489515957No Hit
GACATGGGGGATCTAAGAAATTTTAGTAGTGTAATTCTACTAAACGTATCTT1040.14784277489515957No Hit
TTAGTGGGGAGTGGGGCAGGGGGCCTGCCCTGAGGCTCCCAACCATCCTCTC1030.14642120975193687No Hit
CGAACTGGGAGCCTATGGAAGTAATATACAAATACCGGAAATTTTAGAAAAA1030.14642120975193687No Hit
AGCACAGGATCCATGAAATGACTTGGGTGATAATCCAGTAATTATCAATAGT1030.14642120975193687No Hit
TGTTACGGGGAGGGGACCCTGCTCAAGCCCAACATGGTGACCCCGGGCCATG1030.14642120975193687No Hit
GTGTGCGGGATTTGCTGGCTGTAGTGTTAGCTAAGCAATGACAAGAACTTTC1020.1449996446087142No Hit
GTAACGGGGAGGGAATCAGCAACTAAAAGCTTATATTACTTTATACTTAACA1020.1449996446087142No Hit
CCAAGTGGGATAGGCTGAAGGTATTTAAGCACAACCTCTGCCCAAATCAGTA1000.1421565143222688No Hit
GTTTTTGGGAGCATCTCATCTTAGTGGACAACCCTGATTGCTAGTGAAGATC1000.1421565143222688No Hit
CTTTGTGGGAATGAAAAATTTGTACAGTTTAAACAACAGCTGAAAGAACTAA990.1407349491790461No Hit
TTTGTTGGGGGTGCCCAGTACTTCCTGGACAAGATCGACGTGATCAAGCAGG990.1407349491790461No Hit
AATATAGGGACTTGGGAAAACTCTACCACCAAACTGTTTGCAAGGTTACACA980.13931338403582344No Hit
CGCTATGGGCAACTTGGGGGATAGGAAAGGTTCAATTTAGCATCCTGTTGAC960.13647025374937805No Hit
ATTAACGGGATTTTAATTCCATTACTTTCAGTCTGGAAAACTAAGCTAGTCA950.13504868860615538No Hit
TACCTGGGGAATCACACTCATATTTGTGTAACCAACTTAGAATATAACCAAA940.13362712346293268No Hit
TGCATAGGAAATTCAGCATTATACTAGAGCTCTAAGTCAATGCAATAAAGTA940.13362712346293268No Hit
CTTTAGGGGAAGAGGAGTGGGGAGTAACTGGAGGCAGTCCTAAAGGTGGAAT930.13220555831970998No Hit
TTTTAGGGCTGGCTCAGGGCGCTCTTCCGATCTGCTTTAGGGTGCGACTTCC930.13220555831970998No Hit
ATCACTGGGAGTCCAGCCTTGTGTTCACCGGAGCTTAAGCGTTGCAGCAGGA920.1307839931764873No Hit
ATCGGGGATCTCCCTGTGTGCCTAAATTTTCTCTTCTTATAAGGAAATTGGT920.1307839931764873No Hit
GTATGAGGGGACTCTTATGATACTGATTTTCCTTTAATGTTAATATGCCAGA920.1307839931764873No Hit
ATTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA910.12936242803326461No Hit
AGACCAGGGAGAGCAGTGCTTCTCCATTTGTTTTGCAGAGAAATGTTTTTCA910.12936242803326461No Hit
CATTGGGAATTGTAAGATTGTACCTTTCAAGAAAACTCAACCTTATTTTTTC900.12794086289004192No Hit
AATACTGGGACCAGAAAGAGAGAAACACCTAAGAAGTTAAATAACTGTCAGT900.12794086289004192No Hit
GCGTTTGGGAGGCTTTCTTTCCCAGTGGGTCATTTTTGTCCTAGTTCAGTGT900.12794086289004192No Hit
GCAATAGGGAATCTGATGTGAGTTCTTCCACTTCTTTTGGCACTTTTCTGTA890.12651929774681925No Hit
TTACGAGGGGTGAAATATAACAAAATTTAAGAAAGTATGTAAAATTTAAATT890.12651929774681925No Hit
GCACTAGGGGGGCTAGATTTGGGGATTCCGATGTCATTATTATCTCTGCAAT890.12651929774681925No Hit
ACTAATCGGGAAGGATGATTAGACATCTCACTACTACTACTGACTATCACAC890.12651929774681925No Hit
TATGGCGGGGAGACAGTGAACTAGCATTTGAATACACAAGTCCAAGTCTACT890.12651929774681925No Hit
ATCACGGGGGCAGAGATCTTGAGTCATTAAGGTCTGGTGTCTCAGAAATCTT890.12651929774681925No Hit
TGTATTGGGGACCAAATGCAATTTGGAATTCAGAATTCTTCATATTTTAGAA880.12509773260359655No Hit
GCTTCAGGGTACCAAAAATATTTAACTTGAATCTGATCATGATGAAACAATC880.12509773260359655No Hit
TAAAGAGGGACCACCTTTACTGTACTGTTGGAGCAGATCTTTATTTTACAAG870.12367616746037388No Hit
TTCCAAGGGTACCCTTAGGAGTTGTCTCGTTGTTAGTTTACAGGTTCTCCCC870.12367616746037388No Hit
CCCAACGGGAACAGAAAAGCTTTTGTATGTCTCTTTTTTTGTTTTTTTGTTT870.12367616746037388No Hit
AAACCGGGTAATGTATCAGAAACTGTACTTACAGATTATCAAGTAGGGAACA860.1222546023171512No Hit
AGCACTGGGATGACAAATTACCCTAGAATGGGTGGTTTAAACAATGAATGTT860.1222546023171512No Hit
GCCTGGGGAGAAATTCAGCTGTCATTCAGTTACTCTTAATTCTTTATAAAAC860.1222546023171512No Hit
ATGAATGGGATATGACAAAAGTATATTTGAGTCAAATGTGGCTTTCTAAAAT860.1222546023171512No Hit
ACCAGGGAGTTTGACAGGAGGAGAATAAGGCTGAAAGCCTCAAGTGAAGGAT860.1222546023171512No Hit
GGATACGGGCTTATGTAAGTATTATCTAATGGCATTTTCTTTATCTCTTGAG860.1222546023171512No Hit
ATTCGGGAGGCTGAGGTGGGAGGATCATTTGAGCCCGGGAGGTTGAGGCTGC850.1208330371739285No Hit
GGCAATGGGATGCTAAACTAGTTATCACTTGTCTGTCTGTCTCTGGCCACTT850.1208330371739285No Hit
ACACAAAGGGACAATAGGTCTCCAATATGTATGGCATCAGACTTCTCAGTGG850.1208330371739285No Hit
GTGGTCGGGACCTTGAAATTATTTGACCAAGAAGCTCAAAGAAAAGAAGTAC850.1208330371739285No Hit
ATACGTGGGCTAAGATAAGTGTGTGGGTATGTAAAGTAGCACAGTCATTATG850.1208330371739285No Hit
TTGAAAGCGGGATTTTTGGTGGAGACAAGGTTTCGCTGTGTTGGCCCGCCTG840.11941147203070582No Hit
TTTCAAGGGATTTGGAGGTGGGGCCTTTGGGAGGTGACTAATCATGAGAGTG840.11941147203070582No Hit
TTCATAGGGGCTTTCCAGATGCTAAGAAGCAGCAGTGGGGCTTTTGAATTTT840.11941147203070582No Hit
AGATACGGGATTTTTTTAAATTGTATTAGTAATTTATGTTTAATACAAAAGG830.11798990688748313No Hit
ATCTTCGGGATACCAATCTTAAAGCAAAAGCTTTCATATTTTCCCTATTCAT830.11798990688748313No Hit
TGCAATGGGATCCACCATGAACCAATGCTGTGAGCTGCTTAGTTTGCTTGTT830.11798990688748313No Hit
TTACTCGGGGCTGATATGAATACTTAGTAATAATACAAAAGTTCCTGCTAAA830.11798990688748313No Hit
TAGTGCGGGATCTTGCATGTACCTTTTAAGTATAAATAGACAAGACCATGTA830.11798990688748313No Hit
CGCACAGGGGTTTGCCATTTTGTTAAAGTACATTGCCCCTTAAATTTTGGAG830.11798990688748313No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGT820.11656834174426044No Hit
TAACACGGTTGGTAACTGGAGTAACTACTGTGGGTGTCGCATATGCTGCCAA820.11656834174426044No Hit
AAACGAGGGTGGATGCACCTGGAGGTCATTATTCTGAGCAAATTAACTCAGG820.11656834174426044No Hit
AGTGAGGGATCCTCAGTGCTATTAATATTTTGGGCCACCTAATTCTTTGTTG820.11656834174426044No Hit
AAAGCAGGTCTAGCAGTGGGGGCGGAAGGCTGGGAGGGCTGCGCGGGCTGCG820.11656834174426044No Hit
GCCCATGGGATTGTCATTCATCTCACTCTGGGCACCTGAGGTCCATAAGCGT820.11656834174426044No Hit
GCCATAGGGAGTTGAAGTGTTTTTAAAAACACTTTGTTTTGTAATGTTTTGA820.11656834174426044No Hit
GTGCCAGGGATTCAGTCAAGACTAACCACATTTCCAGTGCTCAGTTACATTA820.11656834174426044No Hit
GATAAAGGGGTAAGAAGAACATTTCTTTGGAAATAGGACTATTGAAAAGCTG810.11514677660103775No Hit
AAACAGGGGAGGGAAGACTGTTGCCGGATGACAAGGCTGTCACTATTGTTCT810.11514677660103775No Hit
TATTCTGGGAGTTAAAAATGTGGATAGATACTGTGAAGCTGCTCGCTCAGAA810.11514677660103775No Hit
ACAAATGGACACATTCTATTCAACAGTATATGGAACTTTCTCAAAGATAGAC810.11514677660103775No Hit
CGCAGTGGGAGAGACCGAAAGTTTCCTGTGTATATCTTTTTGAAGTTATATT810.11514677660103775No Hit
TATCTGGGGAGTCCATTTGTATTGCTGCGAAGGAATACTTGACGCTGGATAA810.11514677660103775No Hit
CATTGAGGGTTGGGTTCCCCATTAAATCGTTTACATACAAGAACACAGTTTT810.11514677660103775No Hit
TGCTACCGGGGCTTATCACTGATAAGAGCCCAATAAAACTATTTGACCATTG800.11372521145781506No Hit
TTCACTGGGGAGCCCTACCCCTTCCAGTATATTGTATTTCTTTATCCACATT790.11230364631459237No Hit
TATTGCCGGGATTTTGAATAAGATACTATTCACGCACATGTCTGTATGTGGA790.11230364631459237No Hit
AAATCAGGGAGCATTGCAGATGACACCAGGGTTCAGGAAAGAGGATGTCTAC790.11230364631459237No Hit
GCAACTGGGACATAGACAAAAATAGTTTGACAAAACTGCAGGGAAGGAAGAG790.11230364631459237No Hit
AATAGCTGGGACAGCAGGGAAAGAGGGTAGAAGTGAGGAGAGTGGGGTGCTA790.11230364631459237No Hit
GACACGGGGAACCTTGGAAGCTGTAGTAATTCTAAGGAATCATGAACCTTGC780.11088208117136969No Hit
GACTGTGGGAGTCCAAATTATTTTTGATTTGTAAATTATTAGGAAAGTTTTT780.11088208117136969No Hit
TCAAAGAGGGCCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCA780.11088208117136969No Hit
TTTGTAGGGAAATATTAACACGGTTCAGGTCAGCACATTAGTTTGTCTTTAT780.11088208117136969No Hit
ATTAGTGGGAACTAAGCAAAATTCACCTTAGTAGAATAATTTAAAAGCTCTT770.10946051602814699No Hit
AAAACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA770.10946051602814699No Hit
GCACACGGGAATTTAAAAAAACCTTCATAATCTGAAATAACAGTTCATTTGA770.10946051602814699No Hit
TATAGCGGGATGATAAAAATGACTTGCTACTTCAAATAGGGACACTAGAGGA770.10946051602814699No Hit
TAGCCGGGAACAACAAAAAACACGAACCGGGACTCCTTGGATAAATGTCTGA770.10946051602814699No Hit
CTTACAGGGGCAGGGTCAAAAGACAGTTACTAGCAATGGGGAATGCTTGTCA770.10946051602814699No Hit
ATCCTGGGAGACCCTGCAAGTTGATTCTCATTAATTGTCAGATTCCACTACA770.10946051602814699No Hit
TGGCATGGGGAAGTCAGGGGTCAGAGACCTGGGTCTGTCACTTAGAAGTTGA770.10946051602814699No Hit
TGTCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA760.10803895088492431No Hit
TATCGTGGGCTAGCTGTGGGACTAATGTTTAAGTTGATTTATCAACAGTATT750.10661738574170163No Hit
AATCTAGGGAAGCCAGGCTCAGAAAGACGGGTCATGCATGTTCTCACTCATA750.10661738574170163No Hit
AATGGTGGGGTTTGAGCCGAACAACTCACCACACTTGGCATCACTAAAAGAA750.10661738574170163No Hit
GTAATTGGGAGGCACTGGTTAACCAAGTTGTACACATTGTACCACATTCAGC750.10661738574170163No Hit
TAAGCAGGGAATCTTTGAAGAGCTTAACTAAGTAGGTAATTGAGATCAACCA750.10661738574170163No Hit
ATTGCTGGGAGTTTATCATCATCATTATTGTTATTCAAAATAAGGGTAAATA750.10661738574170163No Hit
GTTTGAGGGCTTTCCAAGAGGTTTTCAACTGACTTTTCCCATATCCCTCAGA750.10661738574170163No Hit
TTTAACGGGTAAAGGGAATCTCCTTCATCTGGAACTTACATTGCAGCGTACA740.10519582059847893No Hit
GCTAAAGGGACTTGCTATTTTAATCAACATCAAAGACCAGATGATGTGCTTC740.10519582059847893No Hit
CTTAATGGGGAAATCTGAAAGTTCAGAGTCTCTTCTTTCTCAGACAACTGGT740.10519582059847893No Hit
GGCTTGGGGGACATATAAATGGTAAATTATATTTAGGCTTATCCTGTTTTGA740.10519582059847893No Hit
ATTTAAGGGATACTATGTCTTTCTTTAGCCAAAACCTATTGATGTACAAGTC740.10519582059847893No Hit
ATACTAGGGATCCCACACTGCATCTAGCCTTTTGTCCCATTCACTTCTGTGA740.10519582059847893No Hit
ATCTGTGGGAATGAGTAAAGCAGAAAAACTTTTAACCAGCAACATTTCAAGT740.10519582059847893No Hit
TCATAGGAAGTTAGCAATCGGTTCTTCCAAACTCCTGTGGGTTGAATATGGT730.10377425545525625No Hit
CACCACGGGAAGTAAGTTGTAGAGTAGATTCAATGTAGTCACTATAAAATTT730.10377425545525625No Hit
ATTATGGGGCGAGGGCTGCGAGAGCGTCGCCGGCTTCCTGGCCTGCGTGCCC730.10377425545525625No Hit
GCCAAGGGGAGGGGTGGTCAAGGCCACAGAGACCTCTGCCTATAGAAAGGGA730.10377425545525625No Hit
TACATTGGGAAGACAGAAACTGCATGTCTAGTCCTGAGTACCTTACTGACAG730.10377425545525625No Hit
GTGGCCGGAAAGCACTGAATTGGGACTAACATTCTGATAGGTTGCACCCTTA730.10377425545525625No Hit
TCCGCTGGGTCCCTTTCTGAGCCAGGGCCAGGTCGGTCCGGCCGCGGCTTCC730.10377425545525625No Hit
TCAAAAGGGGAGATTGCTGCCATTTTTTGGTGCTTTTTGGACCTCGTCATAA730.10377425545525625No Hit
TGCCATCGGGCTACTTTTCTCAGTGTTATATTTGGTTATGAATGCATTTACC730.10377425545525625No Hit
CACCACCGGGGCGCATGATGAAGAGAGCACCGTGAATCTAGTCTTGACTGCC730.10377425545525625No Hit
AGCTGGGGGTCAGGACTTGTTCATTAGGTTGGCAGCAGAGGGGCAGAAGGAA730.10377425545525625No Hit
CTAAGCGGGCGATCTGCTAAGGGGCACCACAGTATCTCCAATGCCTAGGCAC730.10377425545525625No Hit
GGCGCAGGGATAGGTGCCCAGGTCATCTATAAAAACGATCCTTGGGCTGTGT730.10377425545525625No Hit
TACTGCGGGGTCCTTTAACTTTTTAAGTTGTCTTCAGTGGAGAGTTTTTCTG720.10235269031203355No Hit
ACCAAAGGGTAGTTGTACAATTGAAAATATTCCTTATTATAGAAGTAAAAAA720.10235269031203355No Hit
TAATCCGGGACTATAACCAAAAAAATGTTATGTTTGATTTGAATATGACAGA720.10235269031203355No Hit
TTTCCTGGGGAAGACATTTGCAACTGACTTGGGGAGGACACAGCTTCAGCAC720.10235269031203355No Hit
TCCCGAGGGATTTTTTTGCATGTTCAAGATATTTGTTTTGGTCTTATGTGTG720.10235269031203355No Hit
CTGTCGGGGATGACATTGTCTGAATTTTTACTTTCTTATTCAGAGATAAGAA720.10235269031203355No Hit
AGACTAGGGCATTCTGAACTCTTCTGCTTTCTCCTGCAAATTAAGTTATTGT710.10093112516881086No Hit
CATAGTGGGAAAGTGCTGAGGTTACAGGTGTGAGCCATCGCACCCAGCCTCA710.10093112516881086No Hit
CACACTGGGGAGAAATGACTTTGATAGATGGTTGCTGTCTGAGAAGCCAGCA710.10093112516881086No Hit
TCATCAGGGGGGCTCTGCCTGGGTTGGAACTAGGGGGAGGGATAAGCTCCCT710.10093112516881086No Hit
AACACAGGGGCATGTCTTTCGTCACGTCGGGCAGCACACCTGCTGTGAAATA710.10093112516881086No Hit
TCAATCGGGAAGAAAGAATCTTGGTGGATTTGTGTCTCAAATCAGATAAGAG710.10093112516881086No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)