FastQCFastQC Report
Sat 31 Dec 2022
SRR5848217.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848217.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69479
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGCCGTCT7241.0420414801594726No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGC6340.912505937045726No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTTATACA1610.2317246937923689No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTT1490.21445328804386934No Hit
GTAACGGGGAGGGAATCAGCAACTAAAAGCTTATATTACTTTATACTTAACA1150.16551763842312064No Hit
TAATCCGGGACTATAACCAAAAAAATGTTATGTTTGATTTGAATATGACAGA1110.1597605031736208No Hit
CGAACTGGGAGCCTATGGAAGTAATATACAAATACCGGAAATTTTAGAAAAA1110.1597605031736208No Hit
ATCTTAGGGGAAGACAACCTTGATTTCAGTGTGAATTCAGAGGTGTCTGTGT1110.1597605031736208No Hit
TATAGCGGGATGATAAAAATGACTTGCTACTTCAAATAGGGACACTAGAGGA1080.15544265173649593No Hit
TGCATAGGAAATTCAGCATTATACTAGAGCTCTAAGTCAATGCAATAAAGTA1010.1453676650498712No Hit
AAAACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1000.1439283812374962No Hit
ATCACGGGGGCAGAGATCTTGAGTCATTAAGGTCTGGTGTCTCAGAAATCTT980.14104981361274632No Hit
AGACTAGGGCATTCTGAACTCTTCTGCTTTCTCCTGCAAATTAAGTTATTGT970.13961052980037134No Hit
GTGTGCGGGATTTGCTGGCTGTAGTGTTAGCTAAGCAATGACAAGAACTTTC970.13961052980037134No Hit
CATTGGGAATTGTAAGATTGTACCTTTCAAGAAAACTCAACCTTATTTTTTC960.13817124598799638No Hit
TTCATAGGGATTCTGGTTTTCAACCAAGTATCTATGCTTTTCTGCTCAAACT960.13817124598799638No Hit
AATATAGGGACTTGGGAAAACTCTACCACCAAACTGTTTGCAAGGTTACACA960.13817124598799638No Hit
AAACCGGGTAATGTATCAGAAACTGTACTTACAGATTATCAAGTAGGGAACA950.13673196217562142No Hit
TTTGCGGGGGGAGAGAGAGCCTCGGCTAGGTGTGGAAACAGGAAGAGAACTA950.13673196217562142No Hit
TTTTGGGGGAAATGGAATTGGGCATGAAAAATGTGAACGGCACAAGGCAAAT950.13673196217562142No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGT940.13529267836324643No Hit
ACATTTGGGCCGTCTGAGGAGCAGTGAGGTCTTCAAGTGGCTCATCCCTGAC940.13529267836324643No Hit
TAGAAGGGGACCCACACCTTTTTTTCGTAGGTGGGCTTTTCCTATCAGAGCT940.13529267836324643No Hit
TTACTCGGGGCTGATATGAATACTTAGTAATAATACAAAAGTTCCTGCTAAA940.13529267836324643No Hit
CGCACAGGGGTTTGCCATTTTGTTAAAGTACATTGCCCCTTAAATTTTGGAG930.13385339455087147No Hit
ATACGTGGGCTAAGATAAGTGTGTGGGTATGTAAAGTAGCACAGTCATTATG930.13385339455087147No Hit
TGTATTGGGGACCAAATGCAATTTGGAATTCAGAATTCTTCATATTTTAGAA920.13241411073849654No Hit
ACGAAAGGGGAATTTGTTTTTATTTTTTAACAAGATTTGTGAACTGAATATC910.13097482692612156No Hit
AAAGCAGGTCTAGCAGTGGGGGCGGAAGGCTGGGAGGGCTGCGCGGGCTGCG910.13097482692612156No Hit
TACCTGGGGAATCACACTCATATTTGTGTAACCAACTTAGAATATAACCAAA910.13097482692612156No Hit
TTCCAAGGGTACCCTTAGGAGTTGTCTCGTTGTTAGTTTACAGGTTCTCCCC910.13097482692612156No Hit
GCTTCAGGGATGGCTTCTAGGTTCCCTCCTCCCCCTACCCCATCTCCTACCT890.12809625930137164No Hit
TCGTAGCGGGACTGAAGAAGAAACGTTATAAGAACTAACAGTTTTACAACTT890.12809625930137164No Hit
GCTTCAGGGTACCAAAAATATTTAACTTGAATCTGATCATGATGAAACAATC890.12809625930137164No Hit
TGATGTGGGGATTTTTGCTCATTTAATATTGCTTTTCCTTGTGCCAGTTTTT890.12809625930137164No Hit
CTTTGTGGGAATGAAAAATTTGTACAGTTTAAACAACAGCTGAAAGAACTAA880.12665697548899665No Hit
TTGAAAGCGGGATTTTTGGTGGAGACAAGGTTTCGCTGTGTTGGCCCGCCTG880.12665697548899665No Hit
GCCTGGGGAGAAATTCAGCTGTCATTCAGTTACTCTTAATTCTTTATAAAAC880.12665697548899665No Hit
TGCTACCGGGGCTTATCACTGATAAGAGCCCAATAAAACTATTTGACCATTG880.12665697548899665No Hit
TATCGTGGGCTAGCTGTGGGACTAATGTTTAAGTTGATTTATCAACAGTATT870.12521769167662172No Hit
TACTGCGGGGTCCTTTAACTTTTTAAGTTGTCTTCAGTGGAGAGTTTTTCTG870.12521769167662172No Hit
CCAAGTGGGATAGGCTGAAGGTATTTAAGCACAACCTCTGCCCAAATCAGTA870.12521769167662172No Hit
TTTGTTGGGGGTGCCCAGTACTTCCTGGACAAGATCGACGTGATCAAGCAGG870.12521769167662172No Hit
TGCGGGGATACTTTCATGATATGTAATCCTCTCATTCTTGATTGTATGGAGT870.12521769167662172No Hit
TGTTACGGGGAGGGGACCCTGCTCAAGCCCAACATGGTGACCCCGGGCCATG870.12521769167662172No Hit
TCATCAGGGGGGCTCTGCCTGGGTTGGAACTAGGGGGAGGGATAAGCTCCCT860.12377840786424675No Hit
ACTTTCGGGAGATGTTCAAATTTCAGATCTTACCATCATCCAACTTAAACTG860.12377840786424675No Hit
TAGCCGGGAACAACAAAAAACACGAACCGGGACTCCTTGGATAAATGTCTGA860.12377840786424675No Hit
AATACTGGGACCAGAAAGAGAGAAACACCTAAGAAGTTAAATAACTGTCAGT860.12377840786424675No Hit
TCAAAGAGGGCCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCA860.12377840786424675No Hit
ATTAGTGGGAACTAAGCAAAATTCACCTTAGTAGAATAATTTAAAAGCTCTT850.12233912405187179No Hit
TCCTCTGGGAATGTAGTACAGCCACTACAGAAAATAGTGTGGAGATTCCTTA850.12233912405187179No Hit
GGATACGGGCTTATGTAAGTATTATCTAATGGCATTTTCTTTATCTCTTGAG850.12233912405187179No Hit
GTTATCGGGCAAATATTTTTAAAGAATTTTTATGTTTTCCAAAATTAGGATT850.12233912405187179No Hit
TTACGAGGGGTGAAATATAACAAAATTTAAGAAAGTATGTAAAATTTAAATT840.12089984023949682No Hit
AGATACGGGATTTTTTTAAATTGTATTAGTAATTTATGTTTAATACAAAAGG840.12089984023949682No Hit
TTGTGCAGGGACCAGAGAGGCAGGAGAGCCAGTCTTGCAGGCATGCTGCCAG840.12089984023949682No Hit
GACATGGGGGATCTAAGAAATTTTAGTAGTGTAATTCTACTAAACGTATCTT840.12089984023949682No Hit
TGCAATGGGATCCACCATGAACCAATGCTGTGAGCTGCTTAGTTTGCTTGTT840.12089984023949682No Hit
GCTAAAGGGACTTGCTATTTTAATCAACATCAAAGACCAGATGATGTGCTTC830.11946055642712186No Hit
TGCAGTGGGCCCAACTCTATGTAAGTAGTCATTGTCCTGTAAGTAGTCATTG830.11946055642712186No Hit
TGCCATCGGGCTACTTTTCTCAGTGTTATATTTGGTTATGAATGCATTTACC830.11946055642712186No Hit
GGCGCAGGGATAGGTGCCCAGGTCATCTATAAAAACGATCCTTGGGCTGTGT830.11946055642712186No Hit
ATATGCGGGGATAAATTTACCTTGAAACAGCAGATGAGCAAAAAGAAAATAA820.11802127261474692No Hit
TATTGCCGGGATTTTGAATAAGATACTATTCACGCACATGTCTGTATGTGGA820.11802127261474692No Hit
ATTGCTGGGAGTTTATCATCATCATTATTGTTATTCAAAATAAGGGTAAATA820.11802127261474692No Hit
ACCAGGGAGTTTGACAGGAGGAGAATAAGGCTGAAAGCCTCAAGTGAAGGAT820.11802127261474692No Hit
AGACACGGGTACAGGATTTTGATGATCAGACTCCAAAGTCTAATTAACAGTT820.11802127261474692No Hit
ATTCGGGAGGCTGAGGTGGGAGGATCATTTGAGCCCGGGAGGTTGAGGCTGC810.11658198880237194No Hit
ATCTTCGGGATACCAATCTTAAAGCAAAAGCTTTCATATTTTCCCTATTCAT810.11658198880237194No Hit
AGCACAGGATCCATGAAATGACTTGGGTGATAATCCAGTAATTATCAATAGT810.11658198880237194No Hit
AGACCAGGGAGAGCAGTGCTTCTCCATTTGTTTTGCAGAGAAATGTTTTTCA810.11658198880237194No Hit
AATCTAGGGAAGCCAGGCTCAGAAAGACGGGTCATGCATGTTCTCACTCATA800.11514270498999699No Hit
ATTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA800.11514270498999699No Hit
TTCAACGAGGGGTTTTGAAAACGTGTGATAGGAAAGCAAATGTTGCAATCCT800.11514270498999699No Hit
AATGGTGGGGTTTGAGCCGAACAACTCACCACACTTGGCATCACTAAAAGAA800.11514270498999699No Hit
CACCACCGGGGCGCATGATGAAGAGAGCACCGTGAATCTAGTCTTGACTGCC800.11514270498999699No Hit
GCAATAGGGAATCTGATGTGAGTTCTTCCACTTCTTTTGGCACTTTTCTGTA790.11370342117762201No Hit
AAATACGGGGGTTCCCTGTGGGGCCCCCGAAGAATGATGATACCCCAAATAG790.11370342117762201No Hit
TATTCTGGGAGTTAAAAATGTGGATAGATACTGTGAAGCTGCTCGCTCAGAA790.11370342117762201No Hit
ATTAACGGGATTTTAATTCCATTACTTTCAGTCTGGAAAACTAAGCTAGTCA790.11370342117762201No Hit
AGTGAGGGATCCTCAGTGCTATTAATATTTTGGGCCACCTAATTCTTTGTTG790.11370342117762201No Hit
GTATGAGGGGACTCTTATGATACTGATTTTCCTTTAATGTTAATATGCCAGA790.11370342117762201No Hit
TTTCCTGGGGAAGACATTTGCAACTGACTTGGGGAGGACACAGCTTCAGCAC780.11226413736524704No Hit
GTATATGGGACCCTACAACCATCATGAATGCAGATATTCTAGCATATTGTCA780.11226413736524704No Hit
CGCAGTGGGAGAGACCGAAAGTTTCCTGTGTATATCTTTTTGAAGTTATATT780.11226413736524704No Hit
GTTTGAGGGCTTTCCAAGAGGTTTTCAACTGACTTTTCCCATATCCCTCAGA780.11226413736524704No Hit
TGTGCTGGGATGCACACTGAGTATAACTGACCTTATGAGAACATCATTCTCA780.11226413736524704No Hit
ACGCATGGGACATGTGCAGGATGTGCAGGTTCGTTACATAGGTAAATGTTTT780.11226413736524704No Hit
TTGTCGGGATGTCTGTGGATGACAAAAGGCTATAGCCACTATTAAAGCTACA770.11082485355287208No Hit
AAGCATGGGATGTGGAAGTGGGGCGGGCTTCATAGAGACTTGGGAATGAGCT770.11082485355287208No Hit
ACTACTGGGGCTAGACCAAATAACTTTTCAGTTGGTTTGGATAGAAACATCC770.11082485355287208No Hit
ACTAATCGGGAAGGATGATTAGACATCTCACTACTACTACTGACTATCACAC770.11082485355287208No Hit
GTGTTTGGGGGACTGTGGGAGTCAGACAGCCTGAGATTGTAGTGATTGTGGA760.10938556974049714No Hit
CTTTAGGGGAAGAGGAGTGGGGAGTAACTGGAGGCAGTCCTAAAGGTGGAAT760.10938556974049714No Hit
CGACCTGGGACAACTCCATTGGGAGAGTACAACTGAAAAGTCGTGCCTGGTC760.10938556974049714No Hit
TAAGCAGGGAATCTTTGAAGAGCTTAACTAAGTAGGTAATTGAGATCAACCA760.10938556974049714No Hit
GTGCCAGGGATTCAGTCAAGACTAACCACATTTCCAGTGCTCAGTTACATTA760.10938556974049714No Hit
AAACGAGGGTGGATGCACCTGGAGGTCATTATTCTGAGCAAATTAACTCAGG750.10794628592812217No Hit
GGCAATGGGATGCTAAACTAGTTATCACTTGTCTGTCTGTCTCTGGCCACTT750.10794628592812217No Hit
ACCAGTGGGATATCTGGCCAAACTAACCTTCATAAGTGAAGGAGAAATCCTT750.10794628592812217No Hit
TTACTAGGGGATAACAGTAAGTGGGGGACAAGGAAGCCAAACATGACACTAT750.10794628592812217No Hit
TCCCGAGGGATTTTTTTGCATGTTCAAGATATTTGTTTTGGTCTTATGTGTG750.10794628592812217No Hit
ACCTAAGGGTAATCCAATTATCACACAAGTAAATGTAAAATGATGGCTGTGT750.10794628592812217No Hit
ATGAATGGGCGATGCTATTGCTGATGAAGTTTGCCATCAATATGCAATGCAA750.10794628592812217No Hit
TATTGGAGGGTATCCTGACTTTGGATTTTACTCAGACTGAGTCAGGAAAATT750.10794628592812217No Hit
CATTGAGGGTTGGGTTCCCCATTAAATCGTTTACATACAAGAACACAGTTTT750.10794628592812217No Hit
ATCTGTGGGAATGAGTAAAGCAGAAAAACTTTTAACCAGCAACATTTCAAGT750.10794628592812217No Hit
TCATAGGAAGTTAGCAATCGGTTCTTCCAAACTCCTGTGGGTTGAATATGGT740.1065070021157472No Hit
GCACACGGGAATTTAAAAAAACCTTCATAATCTGAAATAACAGTTCATTTGA740.1065070021157472No Hit
GGTCTGGGGATATATCAACTGAATAGCTTATTGAGTATTGAAGGGTGCCAAG740.1065070021157472No Hit
GGTAAAGGGATAGGTTGGTTTTTAAAAAATAATTTGTAAGAATTGTTTATGT740.1065070021157472No Hit
GGCTTGGGGGACATATAAATGGTAAATTATATTTAGGCTTATCCTGTTTTGA740.1065070021157472No Hit
GATGTCGGGACCACAAATGAAATCAAATAATATATGACCAGCAAAAGTATTT740.1065070021157472No Hit
GTAATTGGGAGGCACTGGTTAACCAAGTTGTACACATTGTACCACATTCAGC740.1065070021157472No Hit
CTAAGCGGGCGATCTGCTAAGGGGCACCACAGTATCTCCAATGCCTAGGCAC740.1065070021157472No Hit
TTCATAGGGAACCCTTTGCTGTCAAAAGAGTTGGCGTTTCCTGTTCTGGGTG730.10506771830337223No Hit
GCGTTTGGGAGGCTTTCTTTCCCAGTGGGTCATTTTTGTCCTAGTTCAGTGT730.10506771830337223No Hit
ATGAATGGGATATGACAAAAGTATATTTGAGTCAAATGTGGCTTTCTAAAAT730.10506771830337223No Hit
TACTACGGGCTTTTCATATGGCATACTTCATTCATATGACATAGTTAGAATC720.10362843449099728No Hit
AAATATGGGAAATGGAACATGTCTAAAACCCAACTCCTTTTCTTTCTTTAAA720.10362843449099728No Hit
AACACAGGGGCATGTCTTTCGTCACGTCGGGCAGCACACCTGCTGTGAAATA720.10362843449099728No Hit
ACAAATGGACACATTCTATTCAACAGTATATGGAACTTTCTCAAAGATAGAC720.10362843449099728No Hit
TGCCGTCGGGAATCTACAGTACTATTTATAACAATGTGTGATATTATTTATA720.10362843449099728No Hit
TGCTACGGGTGAGGGTGAAATTAAGGGAAGGCAAAGTCCAGGCACCAGAGTG720.10362843449099728No Hit
CCCAACGGGAACAGAAAAGCTTTTGTATGTCTCTTTTTTTGTTTTTTTGTTT720.10362843449099728No Hit
CTTATTGGGGATCTATTGACGGGATCTATGAGAGTGCCTCATACCCTTACTG720.10362843449099728No Hit
TACAGAGGGGTTAAGGCTTATTGAAACCTTTCAAGTAGGGGATGGTACATTT720.10362843449099728No Hit
GAAACTGGGTGCACATGTGAGGGATCTAGGTCGCGTGCTCCTTATGAGAATC710.10218915067862233No Hit
CGCTATGGGCAACTTGGGGGATAGGAAAGGTTCAATTTAGCATCCTGTTGAC710.10218915067862233No Hit
TAACACGGTTGGTAACTGGAGTAACTACTGTGGGTGTCGCATATGCTGCCAA710.10218915067862233No Hit
GGTCATGGGAATATGACATTAGGAAATTTAAAACCGCAAGAAAGGAAGGATA710.10218915067862233No Hit
GCTAATGGGGATCTTGCGCAGGGCACCCCAGAGATTTAAGAAGAAAACCTCT710.10218915067862233No Hit
GCTTAAGGGATTTCACTAATTATCCTTTGATAGCTTGGGACTTTGTGTGCCT710.10218915067862233No Hit
CTCTGCGGGACATAGAAAAGATTTTATTTTAAAATGAGTTGTAAAGCTTGTG700.10074986686624736No Hit
AGCACTGGGATGACAAATTACCCTAGAATGGGTGGTTTAAACAATGAATGTT700.10074986686624736No Hit
GCACTAGGGGGGCTAGATTTGGGGATTCCGATGTCATTATTATCTCTGCAAT700.10074986686624736No Hit
GAATTAGGGAAGCTTTATACAAATGGATATCGTGCTGAAGTGGTATTTACCT700.10074986686624736No Hit
TGTTCAGGGGACTTATATAATGGTGCAACAAGAAAACTGGCTCTGCAAAAGA700.10074986686624736No Hit
AAGCGGGGGCTGTCCCATATTTTGTATATTGGAATAAAAATTTCTATAAATT700.10074986686624736No Hit
TCAATCGGGAAGAAAGAATCTTGGTGGATTTGTGTCTCAAATCAGATAAGAG700.10074986686624736No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)