FastQCFastQC Report
Sat 31 Dec 2022
SRR5848216.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848216.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86247
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGCCGTCT8590.9959766716523473No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGC8010.9287279557549828No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTTATACA1920.22261643883265508No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTT1730.20058668707317356No Hit
CTGCTAAAGGGGGAGGCAGTTAGCCTTAGCAAACTAATGCAGGAACAGAAAA1510.17507855345693185No Hit
TCTTGCGGGGGACACCATCGCAGCCTGCGAGGAGATGTTACGGCAGCTGGGC1470.17044071098125152No Hit
TTAGGAGGGATGGCATATGAATTCCTGTTGCTGATGACAAATCATATAAGTT1410.16348394726773105No Hit
GCTGGTGGGTTATGTACAAGGGCTCTTGGGAATGATTCTCTCAGAGGCTTAA1360.15768664417313066No Hit
TTGCACTGGGAACCATCACACTGGCTAACTAAACAGTGCTCAAAGTGGCATC1300.1507298804596102No Hit
ACGCAGGGGAACTGCGAGGAGATTGAGCCCCTTCAGGTGGACGAAGAGGAAG1270.14725149860284997No Hit
CATGTGGGGGTCTAGGAATCTGTTCCTGGAATCCTCAGGGTATACAGATTTC1260.1460920379839299No Hit
GCAAATGGGGGGACCTCCTTATTCCCTGAGACACCACAATATCGAAATTAGA1210.14029473488932948No Hit
TGCACAGGGGTGATTTACTGGGGCTATTGCTAGCAATGTTTTATAAGCAAAT1200.1391352742704094No Hit
CCATCAGGGCACATGAAATAACTTGGCTATATTGAAGAAAATATATTAAAAT1180.13681635303256925No Hit
CACAAAGGGAAACCTTAAATGAAAAAAATGATCTATTGCAGATATTTGATGT1180.13681635303256925No Hit
GACCAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1170.13565689241364917No Hit
CACGGGGGATAGAGAGCTTTTGCATATTCTAAGCTTTAGCATGTTCTCACTT1160.1344974317947291No Hit
AATCAAGGGGATGGAGACATTGGGGCAGCCTTCAGAGACTCCATCAAAGTCA1140.13217851055688895No Hit
CACCCAGGGCTGTCCATTTGACTTGGCAATTGTTAGGAAAAAAGTAAAATAA1130.13101904993796887No Hit
ACGTACGGAAGCATGTAAAGCAAACATTGACAGAACTGAAGTAAGAAATAGA1130.13101904993796887No Hit
CTTGCTGGGGATTTTACAACCAGATAAATAAGTTAATAAGTTAATGAAGGAA1120.12985958931904878No Hit
GCCTATGGGACAAACTCTAAAATTGACCACACAATCAATTCTCGGCTATTGC1120.12985958931904878No Hit
TCTTCTGGGATTCTGAAATCCAGAAATTCTTATTGTATATTTGTTTTTAAAA1110.1287001287001287No Hit
ATTGCGGGGATGAATCTCCTTGAAGACGAAACACTTTAGGATTATAGTTGCT1110.1287001287001287No Hit
GTCACAGGGGGACCGCGTGGGCTCGCCTCCCTGGGACTAGGTTTCAGCGGCC1110.1287001287001287No Hit
AAGCTCGGGGTGCTTGGGATTGGTTGAAGAATAAATTTGTGAAAAGTAGATT1100.12754066808120862No Hit
TACGCGGGGCTAATAGAGCTGGGATTTGAATTCTGGGTATGTGCTACTACTT1090.12638120746228854No Hit
TAGTACGGGAGTGACTGATGACCACCAGTTACTTCATATGAAAGTGGGAAAC1080.12522174684336845No Hit
TCTTCCGGGGTCTAGGAAGAGGCTGGAGATGGGCTTTCTTGGGCAAGGAGGC1080.12522174684336845No Hit
TGCTTTGGGACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG1060.12290282560552832No Hit
GAAATGGGAACAGGGACTAGATTTACCCTCCTGTCAAAAAAAGCAGACAAAA1060.12290282560552832No Hit
CTTGCTAAGGGGAAATACCAGGCCACAGTTTTTTTAAAAATGAGGATGTTCT1050.12174336498660823No Hit
TAGCTAGGGACGTGTAGAATACTTCACAAAATAGTCAACATCTAAGGCCCTA1040.12058390436768815No Hit
TGCATCGGGTCATATAATTTAGTTAATAGCACAGTACAACACAAAACACCTT1040.12058390436768815No Hit
TCAATGGGGCAGACATGAAAAAGTACAAGAGAATTGTATTGATCTTGTTGGT1040.12058390436768815No Hit
GGAGGAGCTGTGTTCTCTGGGGCAGGGAACATCGCAGCAGCCACAGGACTGG1030.11942444374876808No Hit
ACGCTCGGGCAGAACTCAAAGGTGTTCCTATTAGAAAAGATCAGGAGACTAA1010.11710552251092791No Hit
CTAACTGGGGCGGAGGCGGGAGAGGCGAGCTCGCGATGAGTGGTCTCGGCAG1010.11710552251092791No Hit
AGTTGAGGGGGTCCGGAGCCCGGCTCGCTGGGGCAGCATGGCGGGGTCGCCG1000.11594606189200783No Hit
ATTAGAGGGAGTCCTGTCATCAAAGTTACTTGAATTAAATTTTTCTGTGTGA1000.11594606189200783No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGT990.11478660127308775No Hit
GATTAAGGGGTGGGGTTCACTAGGATTTAGTGCCTGATCTTTTTTTTGGGAA990.11478660127308775No Hit
CCCCTTCTGAAGCAGCGAAGGCCACGGTTCCGGCTTCTTTCCTGGCCCCCTC990.11478660127308775No Hit
TAGGCCGGGTTTGGCAATGCAATTTTTTTTAGCAATTAGTTTTTAATTTGGG990.11478660127308775No Hit
CGATGTGGGATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAG990.11478660127308775No Hit
GCTGCTGGGTGATGAGCCAGGTGCGCTATGCTGAGACCATGGAGCTGTGGGA980.11362714065416768No Hit
TAGTCTGGGTACTGCCATCGCGCCTGGTGACAAGCCCCAGATCCAGGAGGTG980.11362714065416768No Hit
GCGTATGGGACAGGTCACTAGGGCAAGCTTCAGGAGAAGTAAAATAGGTGTA980.11362714065416768No Hit
GTGACAGGGGTTACTATTGGCATTCCTTGACTAAAAAGGACCACCTGATGCT980.11362714065416768No Hit
ACGCTTGGGAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAA970.1124676800352476No Hit
AAGTGAGGGCGGTTGAGATCTTGCCTGTCTTCCACCCTGTGGAATGCGGGAG970.1124676800352476No Hit
TTCACAGGGAGTAACTTTTTTTGTAGCACAAAACTGGTTCAGTGTTTACATG970.1124676800352476No Hit
GTATCAGGGAGAGACAGTTTTACTCATAAAACAAATGAAAGATAATTTCAGG970.1124676800352476No Hit
ACGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACC970.1124676800352476No Hit
GTTTCAGGGATTGGAAGCTGCCTTCTAACACTTTGTGTAGCTCTGTGGAGTT970.1124676800352476No Hit
ATAGCCGGGAGAGGTAACATCAGCCCTCAAAAATACTGTCTCAACAGCTGGA960.11130821941632754No Hit
TCCAGTGGGAAGAACAATAAATCACTTTGAACAGCCCATTTTACAAATCAGA960.11130821941632754No Hit
CGCTATGGGGTCCTGGGAGGGCATGGAATCTCTGTGCCACCTCCCCCATACT960.11130821941632754No Hit
TTTGCAGGGAGGTCAGAGTAAATAGAGGAATGAAAAGGAATAGGAAATGACA960.11130821941632754No Hit
TAGCTAGGGATTACATTAACTTTGCGATAAGTGTGCCTGAGTTCTGCCTTCT950.11014875879740746No Hit
TCCACAGGGACATGAAACGGCTTTCCCGGGGCCAAACGGTGGAGGATCTGCT950.11014875879740746No Hit
GCACTTGGGGAAGTTAAATGTTAACAGTGATCAAAAGAAAGCTGGAGTAGCT940.10898929817848738No Hit
TTTGAGGGGATGGTGACCCATGGTCCTTAGGATGACTGCTCAGGGACAATTT940.10898929817848738No Hit
AAGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA940.10898929817848738No Hit
CGGCCGGGGATATGTAGCAGTTATTAATTTTTACTGCTGTATAGCACTACAC940.10898929817848738No Hit
TGGATGGGGCACCCTTATGAGATCTCCAGAACTAGACGCCATGGCTGCATTG940.10898929817848738No Hit
AAACTCGGGAGAGGAGAGACCTTAAGTCTTACTTGCAGCCAAAAGCCTTCAA940.10898929817848738No Hit
TACAATGGGAGCTCTCTCTTCTCATTCATGGCCTGTATCTGACCCATGACTA940.10898929817848738No Hit
TGTACAGGGATGTGCTTAATATTTCCTACTGTGTAGGAGAATTTGCAGTCAG940.10898929817848738No Hit
AGTGCAGGGGATCTGTAAGAGGGTGTCTTTCCATTCTTGATTTCAAGTTTGA940.10898929817848738No Hit
GTTTTTGGGGACCATCCTGGCTAACACGGTGAAACCCCATCTTTACTAAAAA930.1078298375595673No Hit
GCACAAGGGTAAATGATTTCTTAAGTATTAAAAACCTTTTAAAGAACAGAAA930.1078298375595673No Hit
ACAGCGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGG930.1078298375595673No Hit
TCTCAGGGGAATTATGAGGCTAAAGATGTTTTTGACGCTGCACATGTGTGTT930.1078298375595673No Hit
TATGCAGGGACTTAAATGTGATTCCAAAAAGGAGGAAAGAAGTTCAGAAATG920.10667037694064721No Hit
ATGTATGGGCATTTTCCTGACTATATAATAAACCCCTTGAACACAGGGACAA920.10667037694064721No Hit
GGCGTAGGGGAATTTGGGGGAGGAATTTTAAGTAATTAGTTATAAATTAGAG920.10667037694064721No Hit
CTACCCGGGGGTGTTAAAGAGTTCAAGGTAATTTGTGATGCCTGTCAGAAGG920.10667037694064721No Hit
CAAAGTGGGATTTCATTCACTTCCTCCTTACAAGGTGAAATTTCAATCTGTA920.10667037694064721No Hit
AAACAAGGGTGAACCCTAGCAGTCTAGCTTCTGAATCTGACTTATAACTGCT920.10667037694064721No Hit
TGATCTGGGATGAATTGTAAAAACAAACATACTTGTGGGGTCTGATAGCAAA920.10667037694064721No Hit
GCGCTTGGGGCCCAAAATAACTCATTTTGTATTATCTGAAAATATATAATTT910.10551091632172713No Hit
GGTTCAGGGAGTCAATGGGAAACTATTAAACAAAGCAGGAAAAAGAGGTGAT910.10551091632172713No Hit
GTGCTCCAGCCAGCAGAAAGCCAGGGAGAGAACTCTCTCACCAGATCCATGC910.10551091632172713No Hit
GTATGGGGGATTAATAAGGGATGATGGAATCTCACTGAAGATGTATTTCCCT910.10551091632172713No Hit
TTACACGGGAACTTGTTTATCCAGCTCTATCATAGCAGTAACTTTACTAAAA910.10551091632172713No Hit
AAACTTGGGCAAACGGGGTTTTATTGAGAAACAGTCATGTTCATTTATCTAT900.10435145570280706No Hit
ACAAGGGGGACCCTGGCGGGGTCGCAGGGTTGGGATGGCGGCTGGAGGCGAT900.10435145570280706No Hit
GTTTATGGGATCTGTGATGCTTTACCTGGGCAGGTCTAGGCAGGTCCTCCTT900.10435145570280706No Hit
TGCGAATGGGAGAGTACCGGGTGCCAGACTGAACTGCTTCCTCTTTCCTTCA900.10435145570280706No Hit
AAGCATGGGGAAATCGTGATGCAGCTTCCTCAAAGTTGCTTCAAGAAAAGCT900.10435145570280706No Hit
ACAGCAGGGACAGAAACTCAATCCAGAAGTGCATTTTCCAGAGTATGTGTTC890.10319199508388698No Hit
ACACTCGGGCAGCAGACATGATCCTAGTGGATGATGATTTTCAAACCATAAT890.10319199508388698No Hit
CATAGTGGGAATGTCATATATATGTATCTGATACATAGATATATGCACACAT890.10319199508388698No Hit
TTCCCGGGAATGATGCATCTGAGAGAACTAGAGAAGCCAAAGCAAATGAAAC890.10319199508388698No Hit
CTACACGGCAAGCCTTATTGCTCTTCCCATGGAATTCGAATGATGACAATAA890.10319199508388698No Hit
AATTTCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA890.10319199508388698No Hit
CACCTAGGGATGTCTAACTTCTACTACAACTACAGGAACAGTGAGCCTTGCT890.10319199508388698No Hit
TGTCACGGGAACAGCCATGAAGCAAAAAATATATTCTAAACTGAAATAAATA890.10319199508388698No Hit
AGCAAAGGGCAACGTGCAATTTTTACATTGTGTAGTATTATGAATTATTTCA880.10203253446496689No Hit
TTTATGTGGGATTCCCACAAATTGGTATGGTTTGGCTGTATCCCCATGCAAA880.10203253446496689No Hit
GCGTGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA880.10203253446496689No Hit
AACACTGGGGCTTTTGGAGCAACTAGATGCAGGGGGAGCTGGTCTTCCTGCA880.10203253446496689No Hit
GAAGCTGGGTTATGTTCCAATATGAAGCCTTACCCATATGACTCCTCTGTTG880.10203253446496689No Hit
CTCAGACGGGCCTGTACCCAAAGCATTCTACCACAGTTCTATTTGACTCCCA880.10203253446496689No Hit
TGCTTAGGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGA880.10203253446496689No Hit
CTTTGGGGATTTTCAATTGTGGTCATTTTAGTGGATGTCTAGTGGTACTTGT870.10087307384604681No Hit
GATCACGGGGAAGTTATCTTTGGAGAGTATCTTATAACTTAATCTATTTATT870.10087307384604681No Hit
TAACAAGGGATGTGTAACTGTTAAAGAAACCGACAAACTGTTTTCCAAAGTA870.10087307384604681No Hit
TCTCGCAGGGATGGTTCCAGAACCGCCGCGCCAAGGCAAAGAGACTACAAGA870.10087307384604681No Hit
TCACATGGGGCTTCCTGTGCTATCTCTAATAACGAGGAGCAATCATTAAAAG870.10087307384604681No Hit
TAGACGGGGGACAGCGAAATGGCGCCTCCGGCCCCCGGCCCGGCCTCCGGCG870.10087307384604681No Hit
ATCTGGGACATATAACCACGTATGTATATTAATTGGAATTTATAAATTAATT870.10087307384604681No Hit
TAAGCCGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGG870.10087307384604681No Hit
GATAGTGGGTGTAAAGAATGTTTTCTTTTAAAATGTTCTCTTTTGAGAACAA870.10087307384604681No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)