FastQCFastQC Report
Sat 31 Dec 2022
SRR5848215.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848215.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85910
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGC8500.9894075194971482No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGCCGTCT8400.9777674310324759No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTTATACA1820.21184961005703642No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTT1480.1722733092771505No Hit
AATCAAGGGGATGGAGACATTGGGGCAGCCTTCAGAGACTCCATCAAAGTCA1440.1676172738912816No Hit
TTAGGAGGGATGGCATATGAATTCCTGTTGCTGATGACAAATCATATAAGTT1420.1652892561983471No Hit
TCTTCTGGGATTCTGAAATCCAGAAATTCTTATTGTATATTTGTTTTTAAAA1340.15597718542660924No Hit
CTGCTAAAGGGGGAGGCAGTTAGCCTTAGCAAACTAATGCAGGAACAGAAAA1310.15248515888720754No Hit
TCCACAGGGACATGAAACGGCTTTCCCGGGGCCAAACGGTGGAGGATCTGCT1290.15015714119427306No Hit
CCATCAGGGCACATGAAATAACTTGGCTATATTGAAGAAAATATATTAAAAT1280.14899313234780584No Hit
GAAATGGGAACAGGGACTAGATTTACCCTCCTGTCAAAAAAAGCAGACAAAA1240.14433709696193692No Hit
GTATCAGGGAGAGACAGTTTTACTCATAAAACAAATGAAAGATAATTTCAGG1230.14317308811546967No Hit
GCTGGTGGGTTATGTACAAGGGCTCTTGGGAATGATTCTCTCAGAGGCTTAA1230.14317308811546967No Hit
AAACTTGGGCAAACGGGGTTTTATTGAGAAACAGTCATGTTCATTTATCTAT1210.14084507042253522No Hit
GCGTATGGGACAGGTCACTAGGGCAAGCTTCAGGAGAAGTAAAATAGGTGTA1210.14084507042253522No Hit
ACGTACGGAAGCATGTAAAGCAAACATTGACAGAACTGAAGTAAGAAATAGA1200.13968106157606797No Hit
GACCAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1190.13851705272960074No Hit
TCTTGCGGGGGACACCATCGCAGCCTGCGAGGAGATGTTACGGCAGCTGGGC1190.13851705272960074No Hit
TGGATGGGGCACCCTTATGAGATCTCCAGAACTAGACGCCATGGCTGCATTG1180.13735304388313352No Hit
CATGTGGGGGTCTAGGAATCTGTTCCTGGAATCCTCAGGGTATACAGATTTC1180.13735304388313352No Hit
TATGCAGGGACTTAAATGTGATTCCAAAAAGGAGGAAAGAAGTTCAGAAATG1150.13386101734373182No Hit
CGTGCCGGGACATCAGGTGGCTGCACATGGAGGGCAGGTTTGGATGGGGCAT1140.13269700849726457No Hit
ACAAGGGGGACCCTGGCGGGGTCGCAGGGTTGGGATGGCGGCTGGAGGCGAT1140.13269700849726457No Hit
TTCACAGGGAGTAACTTTTTTTGTAGCACAAAACTGGTTCAGTGTTTACATG1130.13153299965079734No Hit
TACGCGGGGCTAATAGAGCTGGGATTTGAATTCTGGGTATGTGCTACTACTT1130.13153299965079734No Hit
GCACTTGGGGAAGTTAAATGTTAACAGTGATCAAAAGAAAGCTGGAGTAGCT1120.13036899080433012No Hit
AGAGGCGGGAGTTGAGTGGGTTTGTGAATGGGAAGAAAATAATTTGTAGACA1120.13036899080433012No Hit
GCCTATGGGACAAACTCTAAAATTGACCACACAATCAATTCTCGGCTATTGC1110.1292049819578629No Hit
GCAAATGGGGGGACCTCCTTATTCCCTGAGACACCACAATATCGAAATTAGA1110.1292049819578629No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGT1100.12804097311139565No Hit
TAGACGGGGGACAGCGAAATGGCGCCTCCGGCCCCCGGCCCGGCCTCCGGCG1100.12804097311139565No Hit
AAACAAGGGTGAACCCTAGCAGTCTAGCTTCTGAATCTGACTTATAACTGCT1090.12687696426492842No Hit
CATGATGGGGATCTGTGCAAGGGATGTATAAGTTCTAAGTTACAGGTTTTGT1090.12687696426492842No Hit
GGCGTAGGGGAATTTGGGGGAGGAATTTTAAGTAATTAGTTATAAATTAGAG1080.1257129554184612No Hit
TTTGCAGGGAGGTCAGAGTAAATAGAGGAATGAAAAGGAATAGGAAATGACA1080.1257129554184612No Hit
TGCACAGGGGTGATTTACTGGGGCTATTGCTAGCAATGTTTTATAAGCAAAT1070.12454894657199395No Hit
ATACATGGGTGACTCTGTCTTCCCTGGGGCAGGACCCCAGCCTGCCTACATT1070.12454894657199395No Hit
CACCTAGGGATGTCTAACTTCTACTACAACTACAGGAACAGTGAGCCTTGCT1070.12454894657199395No Hit
AAGTTTGGGATGCCTTCCTCGGTTACCATAAATCTGCATTATCCTAGGAAAG1070.12454894657199395No Hit
TGCTTTGGGACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG1060.12338493772552671No Hit
CTACCCGGGGGTGTTAAAGAGTTCAAGGTAATTTGTGATGCCTGTCAGAAGG1060.12338493772552671No Hit
CTTGCTAAGGGGAAATACCAGGCCACAGTTTTTTTAAAAATGAGGATGTTCT1060.12338493772552671No Hit
AAGCTCGGGGTGCTTGGGATTGGTTGAAGAATAAATTTGTGAAAAGTAGATT1060.12338493772552671No Hit
TTTGAGGGGATGGTGACCCATGGTCCTTAGGATGACTGCTCAGGGACAATTT1050.12222092887905948No Hit
TCCAGTGGGAAGAACAATAAATCACTTTGAACAGCCCATTTTACAAATCAGA1050.12222092887905948No Hit
AACACTGGGGCTTTTGGAGCAACTAGATGCAGGGGGAGCTGGTCTTCCTGCA1040.12105692003259225No Hit
ATCTGGGACATATAACCACGTATGTATATTAATTGGAATTTATAAATTAATT1040.12105692003259225No Hit
GGATAAGGGGAGTTCCTTGTAACTACCATTAAGTATAGCACTTTTTACGAAA1030.11989291118612501No Hit
TTCAATGGGACATCGTTGACAGGCGGTTCCGTTCTCCAGATGATTCTTGCAA1030.11989291118612501No Hit
GCGTGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1020.11872890233965779No Hit
GCCATTGGGCTGATCGCATTGAAAACATTGATCACCTGGGTTTCTTTATTTA1010.11756489349319053No Hit
ACGCAGGGGAACTGCGAGGAGATTGAGCCCCTTCAGGTGGACGAAGAGGAAG1010.11756489349319053No Hit
ACGCTCGGGCAGAACTCAAAGGTGTTCCTATTAGAAAAGATCAGGAGACTAA1010.11756489349319053No Hit
AGTTGAGGGGGTCCGGAGCCCGGCTCGCTGGGGCAGCATGGCGGGGTCGCCG1010.11756489349319053No Hit
ATTGCGGGGATGAATCTCCTTGAAGACGAAACACTTTAGGATTATAGTTGCT1000.11640088464672331No Hit
TATAATGGGGGGCTCCTCCCTGAGCGCACCGGCCCTTCCTGTCACCCAGGTC1000.11640088464672331No Hit
CGATGTGGGATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAG1000.11640088464672331No Hit
GTTTCAGGGATTGGAAGCTGCCTTCTAACACTTTGTGTAGCTCTGTGGAGTT1000.11640088464672331No Hit
ATTAGAGGGAGTCCTGTCATCAAAGTTACTTGAATTAAATTTTTCTGTGTGA990.11523687580025609No Hit
TCTTTGGGCTAGGTGGGGGCTCCCTGAGCATTTGAGGATCAGAGTTTTTAAA980.11407286695378883No Hit
CTACACGGCAAGCCTTATTGCTCTTCCCATGGAATTCGAATGATGACAATAA980.11407286695378883No Hit
CATGTCGGGGATGTGTTAAGATTAATTCATGTTATATGTTCATGAGAAAAAT980.11407286695378883No Hit
GTTTTTGGGGACCATCCTGGCTAACACGGTGAAACCCCATCTTTACTAAAAA970.11290885810732161No Hit
GCACAAGGGTAAATGATTTCTTAAGTATTAAAAACCTTTTAAAGAACAGAAA970.11290885810732161No Hit
GTGCTCCAGCCAGCAGAAAGCCAGGGAGAGAACTCTCTCACCAGATCCATGC970.11290885810732161No Hit
ACGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACC970.11290885810732161No Hit
TAGGCCGGGTTTGGCAATGCAATTTTTTTTAGCAATTAGTTTTTAATTTGGG970.11290885810732161No Hit
GAATTAGGGGCTGGCTAGGAAGGAAAACATTGGCATTTTTTTTCAGGAAGAA970.11290885810732161No Hit
TCTTCCGGGGTCTAGGAAGAGGCTGGAGATGGGCTTTCTTGGGCAAGGAGGC970.11290885810732161No Hit
CGGCCGGGGATATGTAGCAGTTATTAATTTTTACTGCTGTATAGCACTACAC960.11174484926085439No Hit
CTTGCTGGGGATTTTACAACCAGATAAATAAGTTAATAAGTTAATGAAGGAA960.11174484926085439No Hit
TATCAGGGTGTCATATATTAGTATAGCCACTACAGCTTTCTTTTGATTTGTG960.11174484926085439No Hit
TTCCCGGGAATGATGCATCTGAGAGAACTAGAGAAGCCAAAGCAAATGAAAC960.11174484926085439No Hit
GCAAATGGGGACCAGCAACAATGGATTAGAGGAGGAGTTTAAGAAGTTAACA960.11174484926085439No Hit
AATTTCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA960.11174484926085439No Hit
TTGCACTGGGAACCATCACACTGGCTAACTAAACAGTGCTCAAAGTGGCATC960.11174484926085439No Hit
GCAGCCTGGGGGACAGAGCGAGACTGTCTCAAAAACAAACAAACAAACAAAC960.11174484926085439No Hit
GCTGCTGGGTGATGAGCCAGGTGCGCTATGCTGAGACCATGGAGCTGTGGGA950.11058084041438716No Hit
GAGTGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC950.11058084041438716No Hit
TAGCTAGGGATTACATTAACTTTGCGATAAGTGTGCCTGAGTTCTGCCTTCT950.11058084041438716No Hit
CACAAAGGGAAACCTTAAATGAAAAAAATGATCTATTGCAGATATTTGATGT950.11058084041438716No Hit
CTTGTAGGGACGGACATCCTGCCCAAGTGGCTGCGCTTCATCCGAGGTGTGG950.11058084041438716No Hit
CTCAAAGGGATGTTAAGTTTTACCAGCCTAAAAAAAGTAACAAACCAATTAA940.10941683156791991No Hit
TAGCTAGGGACGTGTAGAATACTTCACAAAATAGTCAACATCTAAGGCCCTA940.10941683156791991No Hit
GGATACGGGATGATTGTTTATCCTAATCCCAATGCTGAAGTAAGATTGTCTT940.10941683156791991No Hit
ACGCTTGGGAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAA930.10825282272145269No Hit
CTGTATGGGTACTACTATCCTAAAATTTTTATGGTGGGAAACAGAATTCTAG930.10825282272145269No Hit
TAGTACGGGAGTGACTGATGACCACCAGTTACTTCATATGAAAGTGGGAAAC930.10825282272145269No Hit
CACCCAGGGCTGTCCATTTGACTTGGCAATTGTTAGGAAAAAAGTAAAATAA920.10708881387498546No Hit
GGCAACGGGCAAGCTTATATGATTCTTGAGGTAACTCTACCAGATACTTCCA920.10708881387498546No Hit
CGCTATGGGGTCCTGGGAGGGCATGGAATCTCTGTGCCACCTCCCCCATACT920.10708881387498546No Hit
GGTTCAGGGAGTCAATGGGAAACTATTAAACAAAGCAGGAAAAAGAGGTGAT910.10592480502851821No Hit
GTCGACGGGATTAAACTCAGTGTCATATCTGAAAGTTCATACTCCTAGGGTC910.10592480502851821No Hit
GGGCATGGGAATAACTGGAGAGAAAGCAAAAATGTCATCAGCTTATTGCCAA910.10592480502851821No Hit
AGCGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA910.10592480502851821No Hit
GAGACAGGGAAAGGGAGAGATGACAGTGTTTCCTGGCAGAGGAGAGTATGTA900.10476079618205099No Hit
TCTCAGGGGAATTATGAGGCTAAAGATGTTTTTGACGCTGCACATGTGTGTT900.10476079618205099No Hit
CGTAACGGGATGTACTGGAAAGCAAAGAAATCTTAGAGTCTTGGACATTGTT890.10359678733558375No Hit
CTAACTGGGGCGGAGGCGGGAGAGGCGAGCTCGCGATGAGTGGTCTCGGCAG890.10359678733558375No Hit
GTGCCCGGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCT890.10359678733558375No Hit
GCCTTAGGGAATGTTAGTATTATTTATTTGACAACTCAGTGTCTAACAGCTT880.10243277848911651No Hit
ATTGCAGGGATTACTGGTTTCAGTATGAGTGAAAAAAATAGTACCTCATGAA880.10243277848911651No Hit
ACCCATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC880.10243277848911651No Hit
AATGTTGGGTCAACACTGAATATGAAGGTTATGTAGGATTGCCATGGCACTT880.10243277848911651No Hit
TCACTTGGGTATCTGTCATGACATCAGACTCAGCACTTAGGGTAAATTTTAT880.10243277848911651No Hit
GGGATCGGGGGCCGGAGGTGGGGGCCGAACCAGCCAAGGTTGCGGGGGCCGC880.10243277848911651No Hit
TCAATGGGGCAGACATGAAAAAGTACAAGAGAATTGTATTGATCTTGTTGGT880.10243277848911651No Hit
ATTTGGGGGGGGAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTCG880.10243277848911651No Hit
CATAGTGGGAATGTCATATATATGTATCTGATACATAGATATATGCACACAT870.10126876964264928No Hit
TGCATCGGGTCATATAATTTAGTTAATAGCACAGTACAACACAAAACACCTT870.10126876964264928No Hit
AATAAGGGGACTGCCATCGCGCCTGGTGACAAGCCCCAGATCCAGGAGGTGC870.10126876964264928No Hit
GGCGATGGGCTGTGAGAGGGGTATGTGATGATGTTCCTTCTACCATCTCATG870.10126876964264928No Hit
TCTTGCGGGGACGCTCTTCCGATCTATGTACGGGATTCTAACATTCTGCTGG870.10126876964264928No Hit
CACATAGGGAGTATTTACATATATTTTATCCATTGATGACTTGCCCAACTTT870.10126876964264928No Hit
AAGCATGGGGAAATCGTGATGCAGCTTCCTCAAAGTTGCTTCAAGAAAAGCT870.10126876964264928No Hit
AAGTGAGGGCGGTTGAGATCTTGCCTGTCTTCCACCCTGTGGAATGCGGGAG860.10010476079618205No Hit
TCATCGCGGGATGATATGCTTGGTTTAAATTCTCTTTGTCCAGGTCAAAAAT860.10010476079618205No Hit
GATTAAGGGGTGGGGTTCACTAGGATTTAGTGCCTGATCTTTTTTTTGGGAA860.10010476079618205No Hit
ATGCCAGGGATCATCTGTCCTCTGGAATGACTCTCCTGTCCCTAAAGGGGTT860.10010476079618205No Hit
ATTACCGGGGGAGTCAAGATGGAGGAGTACGCGCGAGAGCCTTGCCCATGGC860.10010476079618205No Hit
TGAATCGGGTATACATTCCTAAAAGTTTTGTATTTTGTGTTTTGTATTTAAG860.10010476079618205No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)