FastQCFastQC Report
Sat 31 Dec 2022
SRR5848214.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848214.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83822
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGCCGTCT7980.9520173701414903No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGC7890.9412803321323757No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTTATACA1930.23024981508434542No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTT1520.18133664193171242No Hit
ACGTACGGAAGCATGTAAAGCAAACATTGACAGAACTGAAGTAAGAAATAGA1430.17059960392259788No Hit
TTAGGAGGGATGGCATATGAATTCCTGTTGCTGATGACAAATCATATAAGTT1380.16463458280642312No Hit
ACGCAGGGGAACTGCGAGGAGATTGAGCCCCTTCAGGTGGACGAAGAGGAAG1310.1562835532437785No Hit
CTGCTAAAGGGGGAGGCAGTTAGCCTTAGCAAACTAATGCAGGAACAGAAAA1310.1562835532437785No Hit
CACAAAGGGAAACCTTAAATGAAAAAAATGATCTATTGCAGATATTTGATGT1280.15270454057407362No Hit
TCTTGCGGGGGACACCATCGCAGCCTGCGAGGAGATGTTACGGCAGCTGGGC1280.15270454057407362No Hit
GCTGGTGGGTTATGTACAAGGGCTCTTGGGAATGATTCTCTCAGAGGCTTAA1220.14554651523466394No Hit
TCTTCCGGGGTCTAGGAAGAGGCTGGAGATGGGCTTTCTTGGGCAAGGAGGC1190.14196750256495907No Hit
TCTTCTGGGATTCTGAAATCCAGAAATTCTTATTGTATATTTGTTTTTAAAA1180.14077449834172415No Hit
TTGCACTGGGAACCATCACACTGGCTAACTAAACAGTGCTCAAAGTGGCATC1180.14077449834172415No Hit
AATCAAGGGGATGGAGACATTGGGGCAGCCTTCAGAGACTCCATCAAAGTCA1180.14077449834172415No Hit
TGCACAGGGGTGATTTACTGGGGCTATTGCTAGCAATGTTTTATAAGCAAAT1160.13838848989525424No Hit
GCCTATGGGACAAACTCTAAAATTGACCACACAATCAATTCTCGGCTATTGC1160.13838848989525424No Hit
CGATGTGGGATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAG1150.13719548567201928No Hit
TAGCTAGGGACGTGTAGAATACTTCACAAAATAGTCAACATCTAAGGCCCTA1100.13123046455584453No Hit
CTAACTGGGGCGGAGGCGGGAGAGGCGAGCTCGCGATGAGTGGTCTCGGCAG1100.13123046455584453No Hit
CACCCAGGGCTGTCCATTTGACTTGGCAATTGTTAGGAAAAAAGTAAAATAA1070.1276514518861397No Hit
TTTGCAGGGAGGTCAGAGTAAATAGAGGAATGAAAAGGAATAGGAAATGACA1060.12645844766290473No Hit
GAAATGGGAACAGGGACTAGATTTACCCTCCTGTCAAAAAAAGCAGACAAAA1050.12526544343966978No Hit
CACGGGGGATAGAGAGCTTTTGCATATTCTAAGCTTTAGCATGTTCTCACTT1050.12526544343966978No Hit
TGCGAATGGGAGAGTACCGGGTGCCAGACTGAACTGCTTCCTCTTTCCTTCA1050.12526544343966978No Hit
CGGCCGGGGATATGTAGCAGTTATTAATTTTTACTGCTGTATAGCACTACAC1040.12407243921643482No Hit
TAGACGGGGGACAGCGAAATGGCGCCTCCGGCCCCCGGCCCGGCCTCCGGCG1040.12407243921643482No Hit
TTTGAGGGGATGGTGACCCATGGTCCTTAGGATGACTGCTCAGGGACAATTT1020.12168643076996492No Hit
GTGCTCCAGCCAGCAGAAAGCCAGGGAGAGAACTCTCTCACCAGATCCATGC1020.12168643076996492No Hit
AAGCATGGGGAAATCGTGATGCAGCTTCCTCAAAGTTGCTTCAAGAAAAGCT1020.12168643076996492No Hit
TGGATGGGGCACCCTTATGAGATCTCCAGAACTAGACGCCATGGCTGCATTG1010.12049342654672998No Hit
GCAAATGGGGGGACCTCCTTATTCCCTGAGACACCACAATATCGAAATTAGA1010.12049342654672998No Hit
GATCACGGGGAAGTTATCTTTGGAGAGTATCTTATAACTTAATCTATTTATT1000.11930042232349501No Hit
ATTGCGGGGATGAATCTCCTTGAAGACGAAACACTTTAGGATTATAGTTGCT990.11810741810026007No Hit
CTTGCTGGGGATTTTACAACCAGATAAATAAGTTAATAAGTTAATGAAGGAA990.11810741810026007No Hit
AGAGGCGGGAGTTGAGTGGGTTTGTGAATGGGAAGAAAATAATTTGTAGACA990.11810741810026007No Hit
TAGTACGGGAGTGACTGATGACCACCAGTTACTTCATATGAAAGTGGGAAAC990.11810741810026007No Hit
AGATTTGGGGTAATCTGGAACTTCAGGAAGTAAAATTATTTGAAGTTTCTTA990.11810741810026007No Hit
CAGCGTGGGACATGAGAGCCCTCATGTTTCTGGAAGTGTTTAAAAGGTCACC990.11810741810026007No Hit
TACGCGGGGCTAATAGAGCTGGGATTTGAATTCTGGGTATGTGCTACTACTT980.11691441387702511No Hit
TCCAGTGGGAAGAACAATAAATCACTTTGAACAGCCCATTTTACAAATCAGA980.11691441387702511No Hit
CTGTCAGGGGGAATCACTCTTTCACAATCAACTGTTATTTTCATGTGAGAAC980.11691441387702511No Hit
TATCAGGGTGTCATATATTAGTATAGCCACTACAGCTTTCTTTTGATTTGTG970.11572140965379017No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGT960.11452840543055523No Hit
ATAGCCGGGAGAGGTAACATCAGCCCTCAAAAATACTGTCTCAACAGCTGGA960.11452840543055523No Hit
AACTAAGGGATTGTGTGAACATTTTACATTGACTCTAGAAATTTGGCTTCCC960.11452840543055523No Hit
ACAAGGGGGACCCTGGCGGGGTCGCAGGGTTGGGATGGCGGCTGGAGGCGAT960.11452840543055523No Hit
GCGTATGGGACAGGTCACTAGGGCAAGCTTCAGGAGAAGTAAAATAGGTGTA960.11452840543055523No Hit
GGGGCAGGGTTTTAGCTGTTAGGAAAAATATTCCTTGAAAGTATCAACCTTG960.11452840543055523No Hit
TGTCACGGGAACAGCCATGAAGCAAAAAATATATTCTAAACTGAAATAAATA960.11452840543055523No Hit
GCTGCTGGGTGATGAGCCAGGTGCGCTATGCTGAGACCATGGAGCTGTGGGA950.11333540120732027No Hit
TCTTTGGGCTAGGTGGGGGCTCCCTGAGCATTTGAGGATCAGAGTTTTTAAA950.11333540120732027No Hit
AGCAAAGGGCAACGTGCAATTTTTACATTGTGTAGTATTATGAATTATTTCA950.11333540120732027No Hit
CATGTGGGGGTCTAGGAATCTGTTCCTGGAATCCTCAGGGTATACAGATTTC950.11333540120732027No Hit
GGATAAGGGGAGTTCCTTGTAACTACCATTAAGTATAGCACTTTTTACGAAA940.11214239698408533No Hit
ACTTAGGGGACAACTATCAATTTCAGTGCCATAGGCTTGCTATCAAGTCAAT930.11094939276085038No Hit
AATTTCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA930.11094939276085038No Hit
AGCGCAGGGGAGTAGCATTTCTGTGCACCAATAATATTCAAGCTGAGAAAAA930.11094939276085038No Hit
CGCTATGGGGTCCTGGGAGGGCATGGAATCTCTGTGCCACCTCCCCCATACT930.11094939276085038No Hit
ACGCTCGGGCAGAACTCAAAGGTGTTCCTATTAGAAAAGATCAGGAGACTAA930.11094939276085038No Hit
CATTTGGGGATGATGGACAGCATATATGATGGTGGTCCCATAAGATTATAAC930.11094939276085038No Hit
GCGCAGCGGGGGAGGCTACCATCAGAGTGAACGGGCGGCCCGCGGAATGGGA910.10856338431438046No Hit
TGGGTCTGGGGTGTTGAAAATATCCCAGTTTATAGCTTGTCTTTGTTTACAA910.10856338431438046No Hit
GCCATTGGGCTGATCGCATTGAAAACATTGATCACCTGGGTTTCTTTATTTA910.10856338431438046No Hit
GTTCAAGGGGCCAAGATTCTTGCTACAGTTGAGCTATGCAGGCTCAATAATC910.10856338431438046No Hit
TCTTGCGGGGACGCTCTTCCGATCTATGTACGGGATTCTAACATTCTGCTGG910.10856338431438046No Hit
GCGTGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA900.10737038009114552No Hit
GTCGACGGGATTAAACTCAGTGTCATATCTGAAAGTTCATACTCCTAGGGTC900.10737038009114552No Hit
TAGTCTGGGTACTGCCATCGCGCCTGGTGACAAGCCCCAGATCCAGGAGGTG900.10737038009114552No Hit
AAACAAGGGTGAACCCTAGCAGTCTAGCTTCTGAATCTGACTTATAACTGCT900.10737038009114552No Hit
TGATCTGGGATGAATTGTAAAAACAAACATACTTGTGGGGTCTGATAGCAAA900.10737038009114552No Hit
ATTAGAGGGAGTCCTGTCATCAAAGTTACTTGAATTAAATTTTTCTGTGTGA900.10737038009114552No Hit
TCCGATGGGGGCTTCGTTGGCCATGACCAGCTGGCTGGCAGTGACGACAGCG890.10617737586791057No Hit
TATGCAGGGACTTAAATGTGATTCCAAAAAGGAGGAAAGAAGTTCAGAAATG890.10617737586791057No Hit
CATAGTGGGAATGTCATATATATGTATCTGATACATAGATATATGCACACAT890.10617737586791057No Hit
AATAAGGGGACTGCCATCGCGCCTGGTGACAAGCCCCAGATCCAGGAGGTGC890.10617737586791057No Hit
GATTAAGGGGTGGGGTTCACTAGGATTTAGTGCCTGATCTTTTTTTTGGGAA890.10617737586791057No Hit
CATGATGGGGATCTGTGCAAGGGATGTATAAGTTCTAAGTTACAGGTTTTGT890.10617737586791057No Hit
CTTTCAGGGAGACAAGAAATTTGAAGTAACCATAAATCAGCCATTGTACTAT890.10617737586791057No Hit
AACACAGGGGTTAAGGGCAGCCAGGGAGAAAAATCAGGTCACCTACAAAGGG890.10617737586791057No Hit
GCACTTGGGGAAGTTAAATGTTAACAGTGATCAAAAGAAAGCTGGAGTAGCT880.10498437164467563No Hit
AAACTTGGGCAAACGGGGTTTTATTGAGAAACAGTCATGTTCATTTATCTAT880.10498437164467563No Hit
GTATCAGGGAGAGACAGTTTTACTCATAAAACAAATGAAAGATAATTTCAGG880.10498437164467563No Hit
GACCAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC880.10498437164467563No Hit
TTCCCGGGAATGATGCATCTGAGAGAACTAGAGAAGCCAAAGCAAATGAAAC880.10498437164467563No Hit
CTAGTCGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA880.10498437164467563No Hit
CTTGTAGGGACGGACATCCTGCCCAAGTGGCTGCGCTTCATCCGAGGTGTGG880.10498437164467563No Hit
GCAGCCTGGGGGACAGAGCGAGACTGTCTCAAAAACAAACAAACAAACAAAC880.10498437164467563No Hit
AAGCTCGGGGTGCTTGGGATTGGTTGAAGAATAAATTTGTGAAAAGTAGATT880.10498437164467563No Hit
CGATAAGGGAATTCAAAGTCCAATTTCCTGGTTAAAGCTCATGAGTTTTAGG870.10379136742144067No Hit
AAGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA870.10379136742144067No Hit
CTTGCTAAGGGGAAATACCAGGCCACAGTTTTTTTAAAAATGAGGATGTTCT870.10379136742144067No Hit
AAAGCAGGGAGATTATTCTGTTCTCCAAATCTTTTAACGTTAATTTCATATT870.10379136742144067No Hit
TTTTATGGGATCCACAAGTATCTTTTAGACCAGGCTACCAACCTTTTTCCTG870.10379136742144067No Hit
GTATGGGGGATTAATAAGGGATGATGGAATCTCACTGAAGATGTATTTCCCT870.10379136742144067No Hit
GGATACGGGATGATTGTTTATCCTAATCCCAATGCTGAAGTAAGATTGTCTT870.10379136742144067No Hit
CTTTCCGGGATACTAAAGAGGCGAGGCAATGACTGTTGGCCAGTTCTCACCG860.10259836319820573No Hit
TGTACAGGGATGTGCTTAATATTTCCTACTGTGTAGGAGAATTTGCAGTCAG860.10259836319820573No Hit
AGCAATTGGGGATTGAAGATTGAGAGCAACTGATGTAGATCGTTTGGAGGTG860.10259836319820573No Hit
ACGCTTGGGAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAA850.10140535897497076No Hit
ACAGCAGGGACAGAAACTCAATCCAGAAGTGCATTTTCCAGAGTATGTGTTC850.10140535897497076No Hit
GCCATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG850.10140535897497076No Hit
GCAAATGGGGACCAGCAACAATGGATTAGAGGAGGAGTTTAAGAAGTTAACA850.10140535897497076No Hit
CCCCTTCTGAAGCAGCGAAGGCCACGGTTCCGGCTTCTTTCCTGGCCCCCTC850.10140535897497076No Hit
ACGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACC850.10140535897497076No Hit
TTGTTGGGGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGA850.10140535897497076No Hit
GATAGTGGGTGTAAAGAATGTTTTCTTTTAAAATGTTCTCTTTTGAGAACAA850.10140535897497076No Hit
CCATCAGGGCACATGAAATAACTTGGCTATATTGAAGAAAATATATTAAAAT840.10021235475173582No Hit
ACGACATGGGCAGTGTTGATGTGGGTATTTACCTATGTTGGTGCCTTGTTTA840.10021235475173582No Hit
CGTGCCGGGACATCAGGTGGCTGCACATGGAGGGCAGGTTTGGATGGGGCAT840.10021235475173582No Hit
ATACATGGGTGACTCTGTCTTCCCTGGGGCAGGACCCCAGCCTGCCTACATT840.10021235475173582No Hit
ACCTATGGGTCTCTCTCTGGGGTGAGTAGGGTTGAGGCCCAAGGGATGCTCA840.10021235475173582No Hit
AACGCAGGGATGCTTATATTTGATTGACCTTAATCATCAGGAATATTGGGAG840.10021235475173582No Hit
GTGTTCGGGGGTTTGGTTCTGGACCACTGTGGTAAATATTGCAATAAAGCAA840.10021235475173582No Hit
CTGATAGGGTACTTGAATAGTTACAGCATATGTTTGAACAGGAAGTAGGAAC840.10021235475173582No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)