FastQCFastQC Report
Sat 31 Dec 2022
SRR5848196.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848196.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82789
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGCCGTCT6800.8213651572068752No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTCGTATGC5810.7017840534370507No Hit
AAATGCGGGCATGAGAACTGAAGGAGTACCAGAACTGAATGAATGAAGTACT2130.257280556595683No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTTATACA1730.20896495911292565No Hit
GAACTAGGGACTAGAAATGAGTTGGAAAGCTATGCCTATTCTCTAAAGAATC1580.19084661005689163No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGTATCTGTCTCTT1520.183599270434478No Hit
GTAGGCGGGACACATGAACGACTGAATTAGAAATGGAAATGCCTGGCACAGC1350.16306514150430612No Hit
GTGATAGGGGTGTTGCTTCAGCAAGAGTGGCCACGGGGCGAGAATCTTTTCC1260.15219413207068572No Hit
TGTCGGCGGGGAAAAATACAGGAAAGCTATGGAGATGTCTATGGAATTTGCA1260.15219413207068572No Hit
ATGTTTGGGGAGTAAAGTTGAGGCAAACCCTAATGAGCTTTCCCAATGCAAG1240.14977835219654786No Hit
GCTTTTGGGCCACCATGCTAATCATCTCCATGCCAGTGTTGTAAGGACCCTT1240.14977835219654786No Hit
CAACACGGGCAGAAAGCATACATCTAAGGGAACATTGTCCTTCAAAGTAGAC1230.1485704622594789No Hit
TCAAATGGGATTTCATAGAAAGTAGCAATTTTTCAAATTGAACCCAGTAAAT1230.1485704622594789No Hit
AATGCTGGGGTTTGTTACACGAGGTCTGAGAGACAGAGGCAGCGTGTTTGAG1210.14615468238534104No Hit
ACTAATGGGCTCTAACTTGTGTGCACTACAGTTGTTCACCAGGGCCAGTGAT1190.1437389025112032No Hit
CAGTCAGGGGTCCCCAACTACTGTCAAACATTTACTTTTTAACTCCTCATGA1180.14253101257413425No Hit
AAGCTAGGGATTTGTAAATCTTTTCTCCTGTTTTGTGTGTGGCCATTTTTTT1160.14011523269999637No Hit
GGCTTGGGGCCGATCTAGAAGTGGGTAGGGGATGCCCAGTGAATGTGCACTG1150.13890734276292743No Hit
AGCTTGGGTCTCCTATGTGACTTGTCCATCAATTATTCCAATTCAAAGGTCT1130.13649156288878958No Hit
CAGAAGGGATTTTTTACAATGTCTTTTGATAAGGTGAATTTTAAAAAATGAT1130.13649156288878958No Hit
AGGTCTGGGGGTAATCAAAAACTTGGTTCTTTTAAAAGATTAACAAAATTGA1130.13649156288878958No Hit
TAGCACGGGGTTCCTTGAAAGGAAGGGACTGTGCAAACACAAAGCAATATTC1120.13528367295172064No Hit
GCTATGGGGAGCTACGGCCGGAAGATGGCGGCGGCCGCAGAGTTGTCGCTAC1120.13528367295172064No Hit
TTACCAGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG1120.13528367295172064No Hit
CACAATGGGACCCTTCCCTCTTCAGGAAAGACAGGGGGGAGATGTTTTAATT1110.1340757830146517No Hit
TTCTGTGGGCTTTACTGCATTAATGTCAAATTATTAGAATTGTATTCAAATA1110.1340757830146517No Hit
GGCGCTGGGATCAAAGATGAAGTATATGTTGCTATGTCAAAAGCCAAAGCAC1110.1340757830146517No Hit
TTGACTGGGGTCCCTCGGCTGAGAAACCCATTTCTGGATGACAGAGAATGTG1100.1328678930775828No Hit
ACGTTTGGGAAGGTGAGTTTCTTGGTAGCTTGAATAAGAATTAGATCATAAA1100.1328678930775828No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAACGT1080.1304521132034449No Hit
ATTATAGGGTCTGTGTACTTGTAGCAGAAGAAATTGGACTAGGAACTTGCCC1080.1304521132034449No Hit
GACTCTGGGCATGTACTCATTAAGTTTGAAGAACGAGTTAAAGTTACCAAGA1080.1304521132034449No Hit
ACCTTGGGGATAGTCAGTGTCGTTTATATTGTAGCAGTTACAGCTCTGTAGT1070.12924422326637597No Hit
CAATGAGGGTTGTTGGAAGCTTACCAAGAGATTTTGAACTATCCAATTATGA1070.12924422326637597No Hit
AAAACAGGGACCATACAATTCCTAAATTCTGTGAGATCCTTGAAGAAATATT1060.12803633332930703No Hit
GAACTAGGGATGGGAATTCAAGATGAGATCTGAGTGGGGACACAGCCAAACC1060.12803633332930703No Hit
TACACAGGGAAATGGCAGAAGAGAAACTGACCCACAAAATGGAAGCTAATAA1050.12682844339223812No Hit
GGCCCTGGGAGTTGAATTGAATGTCTATAAGGTCTAAAGGTAGAATGTGAAT1050.12682844339223812No Hit
ATTCATGGGGCAGAATTTTCATTTTGACTAGAATCCATTGCTAGAGAGCTAG1040.12562055345516915No Hit
ACTAATGGGGTGAAAACAAACCAAGATGATTTATAGTAAATCTGTGTTAGAG1040.12562055345516915No Hit
ACGCGTGGGCGATCTTGACCTGGGAGGGATTTGATAAGTGTTCATTACAGTT1040.12562055345516915No Hit
TCTGCAGGGTAAATGAAGACACTTTGTCATGAAGGTTGTAAAGCACCAAATA1040.12562055345516915No Hit
AGCATGGGGCATCAAAGAACTTCATCAAAGGACTTAAACTTCTTAAAGGGCT1030.12441266351810024No Hit
TGCGACGGGGTTGGAGGAGCAGTGGTGACGGGTGTGACAGCAGTAGCCCAGA1030.12441266351810024No Hit
AGAACAAGGGGACGAGTTGGTGGGTGCAGCGCACCAGCATGGCACATGTATA1020.12320477358103128No Hit
CTTGCTGGGAGATACATCGTATTCTCACTAAGATGGTTAAAAGACAGGTAAC1010.12199688364396236No Hit
CTTAACTGGGGGTTTGTCATGGGGGAACTGCCCTTTAAATTTTAAGTGACAC1010.12199688364396236No Hit
ATCGCGGGCATAGAAGTCAAGTTTGCCTCCGAAGCAGAAGATAAAATCAGGG1010.12199688364396236No Hit
ATTAGTGGGTAACATAATAAAAACAGTATTTGAGAAAAGGCAAAAACTTTTT1000.12078899370689343No Hit
TGATGGGGGGCCGCCGCCATGGGAGTGCAGGTGGAAACCATCTCCCCAGGAG1000.12078899370689343No Hit
GTTATAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1000.12078899370689343No Hit
GACCACGGAGCCCCAAACTAGAAACAACCCAGATATCCATCGATTGGTGAAA1000.12078899370689343No Hit
GTGTTTGGGTGATTTAAAAATGATCCCCAAAATAATACTACCTCACAGGATC1000.12078899370689343No Hit
GTCACAGGGGCTTTCTTTGCTGCGTCTACTGCGAGAATGAAGACTATTCTCA1000.12078899370689343No Hit
TCTGGCGGGATTCTGAAACTGTTTCCCTTACAGTCAAGTTAAACAAGCAAGC1000.12078899370689343No Hit
ATTCTAGGGTGCCTATAACATCCTGGCTAACCAGAGGTCCTGAGTCTGTCCA1000.12078899370689343No Hit
TCTTAGGGCTCTTTTTTCTAGCTTTGAAACACAGGTATCTAAATTTAAGAAA990.11958110376982449No Hit
ATTTTTGGGTGTCAGAGCAGTTTGGTCTAGAATTGTCTTGTGACAGCAATTA990.11958110376982449No Hit
ACAAATGGGGAATAACAATAAAAAGATAGGAAGTATTTGAAAAGAGCCCAGT990.11958110376982449No Hit
GCCTTGGGGGAGATCTGAAAACAGAGGAGCAGGTAATTAGGGACCCAAAGAA990.11958110376982449No Hit
CTCCTCGGGAGAGCAGAACTAAATGAAATTGAAACAAACAAACAATACAAAA990.11958110376982449No Hit
TCTCAGGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA990.11958110376982449No Hit
GTGTTTGGGATGCTATGTTGAGGGGAGGCAAGTCTGATCTTCGAATAATTGA980.11837321383275556No Hit
GCTTGCGGGAAGAGAAAAATCATTAAACATGGTTTCCTTAGCTTCTACTGGC980.11837321383275556No Hit
TTCTCTCGGGGAAGTGAATTTAACTAGATGTAGTAACCTTTTTTTTCTTTAC980.11837321383275556No Hit
TTCTTTGGGCGATCTTTGGATGGGCCTTCCTTTCGGCCGGAACCGCCATCTT980.11837321383275556No Hit
TCGCCGGGAGGTGAGAAAGGAACTTAGACAAAGGGCACAGAGTTGGATGAAA980.11837321383275556No Hit
TGCCCTGGGATAGACTCATTCCGTACACACACATAGACACATTCCATACACA970.11716532389568661No Hit
TAGCAAGGGATGAGAATCCCAGGGGAGTCCTAAAAGTGGATGCATTTTCTCT970.11716532389568661No Hit
GAATATGGGGATAGGTTAAATAATGGTTTCAGCATAGTGTTTGTTTTTTAGA970.11716532389568661No Hit
AATTGCGGGATTGCAAAAGACCAAGGAATTAACCTGGGTTAGGAAAGATGGA970.11716532389568661No Hit
TGCCTGGGAAATTTAGAAATTTGAAGCTACGAAATTAAGTTTGAATATGGAA970.11716532389568661No Hit
CTTCAGGGGAACAGAATGGAATTTTTTTTCAACTGTATGTAGGGCTGCAGTG970.11716532389568661No Hit
CACGGTGGGCATTCTGTTCCCTATGTTATTTCTTCTTTCAAGCATGAATTAA970.11716532389568661No Hit
ATCGGGGAGCACAAGTAGGGTAACAAGTTTGAAAAACTTATTTTAATTTTAA960.11595743395861768No Hit
TCAATGGGGGTCCAAATAATTTGTATTTGAAACATCTCTATACTTGTTATTC960.11595743395861768No Hit
GCATTTGGGGCTCAGTAAATCTACATTTTACGTAAGATAAAGTAAGCATGTG960.11595743395861768No Hit
GTAAACGGATAGAAATAGGAACTTTCTTTTCATTTCTTATTCATTTATGAAG960.11595743395861768No Hit
TCAACCGGGAAGGTGATAAATTTAAGCCCAATAATTACACTAACTAAAAATG960.11595743395861768No Hit
TGCAAATAGGGGAGATCATCTGAGATGTCTTTTTGCTTACAGACCACTCAGT960.11595743395861768No Hit
ATCATGGGTTGCAGATGTTCAATACATTTGCATTAATAATTTATTTTAACTT950.11474954402154877No Hit
TTAGCAGGGATGCTCTTTTGTGCCAACCTGAGGATAATTTTAACAAAATGTA950.11474954402154877No Hit
ATGTGAGGGTCTCCAGTTGGGATGAAGCCTGCATATACTGTGAGCTGTGATT950.11474954402154877No Hit
TCTAAAGGGCAAGTATTGAGATAAGGGAATATAAATAGTATTATATGTATCA940.11354165408447982No Hit
TTTCTTGGGAGGTAAATGTGTTGAAAAAAGAACAGAATTGACACAGCTTCCT940.11354165408447982No Hit
AAGCCAGGGATCCCTGGTCAGTAAGAATTATGAGATTGAACGGACTATTGTT940.11354165408447982No Hit
TTGCCAGGGAGGCTACACAGCAGGAGGTGAGCAGCCAGCCAGTGAGCAAAGC940.11354165408447982No Hit
GCCACGAGGGGTTCTTTAAGTTCAATGAAGTCTGCCAGGAACATTGGTTGGT940.11354165408447982No Hit
TGTTGTGGGAGGTCCTGGCACCTCTGCTGAGTGCCCAGCCTGAGATGACACA930.11233376414741089No Hit
TGATGCGGGATAGGTTTGATTTATATTGCCTTCATCATTCTGGTTTCAGACG930.11233376414741089No Hit
ACGCCTGGGATACCATTATCTATATGAGCACTATCTGAAGAGTCTCCCACAG930.11233376414741089No Hit
GGATACGGGCTAGCCCTTTTATCAACTCCTAGAATAGTTAAAGAGAGACACA930.11233376414741089No Hit
CTCGCGGGCGATCTGTACTGGGGTATTAGAATCACATGGATAACTTCTAGAA930.11233376414741089No Hit
GGCTAGGGGGGTGTCAATCCTGTTCATGTCTGATTTGGGAGAAGCACAAGGG930.11233376414741089No Hit
GTCATGGGGGTTTGTTCCTGTGAGGTGGCTCCAACATATTTTTCAGTGTCTG930.11233376414741089No Hit
CTAAGGGGGCATACATTACTCCACGCTACAAATAATTAACTATCTATTTGAT920.11112587421034195No Hit
ACTTCTGGGGGAGGCCTGGTTTCCGGGCTCAGGAGCCTTCCGTGAGTTGGTC920.11112587421034195No Hit
TGTTGATGGGATCTTTTTTATTATTAAAAGTATAGATTAAATTAATAATCAT920.11112587421034195No Hit
AAGAATGGGGACGGCACCATTGACTTCCGAGAGTTCATCTGCGCTCTGTCCA910.10991798427327303No Hit
AGAGACGGGGTGACAAGGCAGGACAAGAGCCTGTTTCGCTTTCCTCCCTGAC910.10991798427327303No Hit
GGGCATGGGATTGGAATTTTAAAAGCATGTTACAAATATTAAGGAACTGTTA910.10991798427327303No Hit
ACAATTGGGTCTAAATTTTATTTTGACTCCTTTCTTGGCTTTTTCAAACTGG910.10991798427327303No Hit
CACATTTGTAGCAGTACCAATTTTCGTGGTTGATTTTTCTCCAGCAGTGAGT910.10991798427327303No Hit
TTAAAAGGGTCATCCCTTTGTCCAGTGTATCCACAATGTATACACTCATTAT900.10871009433620407No Hit
TTGGCCTGGGATCATAAGCAGACTTGCCTTTTCAGATATTGGTATATTTTAA900.10871009433620407No Hit
GAACTGGGGGCATCAAATTTCATTGACTGGAATGTAACATGTATAAAGTGAG900.10871009433620407No Hit
TTCTGCGGGAACAAAGGCACTTACAAGCAAAATACTTTTTCTTCTAAAAACA900.10871009433620407No Hit
GGCTCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC900.10871009433620407No Hit
GTCACCGGGTCTACTTCAATAAATATAATTAAGTTTTGTGAAAGCCGTGTTC900.10871009433620407No Hit
TAATTGGGGAAGTGGTAAAACCCCTGGGAATTAAGCTGAGCAGGAACATACA900.10871009433620407No Hit
GGCATAGGGAGCTGCACCTAAAGAGGAAATTGGGTTTATAAGAAAATGGAGG900.10871009433620407No Hit
CGTCAAGGGTATTCTCACGCACAGTAGAGACTCAAGAAGTTAGAAAATTTCA890.10750220439913515No Hit
CCAATAGGGATCTTCAAATTCCCAGCAGCTATTGGTTCCTGCTACATAGTAG890.10750220439913515No Hit
GTTACTGGGCAGGTCTGACTGACCACCAGTCAGAGGCACTTTATTTGTATCA890.10750220439913515No Hit
GGTGCTGGGATTACAGGCATGAGCCACCGCGCCCGGCCTGCCCCTTTCCAAG890.10750220439913515No Hit
TTGTCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA880.10629431446206622No Hit
ATTAGTGGGATCCCAAGTGAAGCTAATTGGTAATTGGTGTTTCATGTCAGAG880.10629431446206622No Hit
GAAAAGGGGAACAACAGCTGGTGCCCTGCTTTCAGATACTGGATTGTACTTT880.10629431446206622No Hit
CTCAGTGGGCTGGCGCCGTTGGCATCGTAGAGTGAACACAACCCAAAAACGA880.10629431446206622No Hit
CTTTGCGGGAACATTCAAGAATTGCTTGAGGATACAAATGTGCGTCTGAATA870.10508642452499728No Hit
GCCTTGGGGAACCAGCACTCACTCAACTTTAGTTAGCAATGGATGTTCCTTC870.10508642452499728No Hit
TTTGAGGGGGGCACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGT870.10508642452499728No Hit
AAAACTGGGTCTGAAAGCACAAGGTGATACTCATTTTTATGGTCTTCCCATT870.10508642452499728No Hit
ACTAGTGGGAATTTAAGCATTTTCATATGTGATGAAATTTAATATCTATAAC870.10508642452499728No Hit
GCTCACGAGGGGAGAAGGGTCATGAGGATGAACTGTGCAGTCACCTGGCAGG870.10508642452499728No Hit
AAAATAGGGCGTGCTCGCCATGTGCTCCAATCATAGGAGAATATGCTGGAGA870.10508642452499728No Hit
TTCTCTGGGAGACTGTGGTATTAATATTTCCACTTATATTTTCATGCACAGT870.10508642452499728No Hit
CTTGCAGGGTCCAAGACACTGTACCTACCATGCAGGAGAACAGTTCTGCTGA870.10508642452499728No Hit
ATACTGGGGCATTTTAATATCTTTCGAATTTCTAGGAAACATTCTATTGATT860.10387853458792835No Hit
GCAGTCTGGGGTAATAACAGGCAGGGATTTGTCCCAGGATAACAATGTGTTT860.10387853458792835No Hit
GTAAAGGGTCGATCTGAAGCCGGGAGATGTACCTAGTGTGTATTTCTTATCT860.10387853458792835No Hit
TCAGTCGGGGGCACGTGACAGTATAGCACCCATTTAAATTTAAATAAAAGTG860.10387853458792835No Hit
ACGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACC860.10387853458792835No Hit
GAGTCAGGGGGGCAGCTACAAGCTGCAGACTTCAATCTACCACACATACTAA860.10387853458792835No Hit
ACTTAAGGGGTTAATTAAGTTTTCAACATAATTTGAACTCTTGTCTTGGCTT860.10387853458792835No Hit
TGCCATGGGGAGGAGTGGGCAGATTTTCTTTGTCTTTTGCTTGCATTTTCTA860.10387853458792835No Hit
TTAACAGGGTAGGGTGCCCATTTCAGCTTTTGACATTGCCCCTGTACTTTTA850.1026706446508594No Hit
GGATGGGGGGTCTGATATTTTAGTATATAGTAGAACATACTTTTTTTTTCTT850.1026706446508594No Hit
TGCACAGGGGGCTCTTTCAGCTGAATGATATAAGAGAGCATTTCACTGGAAA850.1026706446508594No Hit
CATGGTGGGGCAAGAAAAATCTGACCAGCTAGATGCATCATGCCAAAATAAA850.1026706446508594No Hit
CTTCACGGGAGAGGGTGAGAAGTGAATGTAACAGGTATAAATAACATTTTTA850.1026706446508594No Hit
TGCAATGGGTACTGATTACAGATGTACAATTTAGCTTAATCAGAAAGCCTCT850.1026706446508594No Hit
ATATGGGGGTAGCTATTAGTAAAAAGCATTTTGTTATACAACTTCTGAATAA850.1026706446508594No Hit
CCTAACGGGGTAATAAGCCCCTGAGGACTATGCCTTTAATATCTGGAACAAG850.1026706446508594No Hit
CTTCAGGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTG850.1026706446508594No Hit
TTTATGGGGTAACCAGTACTTTTAAACTTAGGAAGTCCAGGGAAGGAAGTAA850.1026706446508594No Hit
TTAATGGGGCAGGAAAGTGAAGGCGAAATCCATTGTCTTCCACCGAAAGAAG840.10146275471379047No Hit
CAATTAGGGGGGTGAAACAGTGCGCCTCATGGATCTTCTGGGAGAAGGACTA840.10146275471379047No Hit
GTCAAGGGGGTTTTAGGATATTTCATCCAGTGCATTCTGACGGATTTCTTAT840.10146275471379047No Hit
CAATCGGGGACTAAGTATTAAGCATGGACAGCTGTTGTTAGAGTAGCAGATT840.10146275471379047No Hit
CTTGGATGGGATAATATTGATTGATAATGCTGTTCAGGTCATCTATGTCTTT840.10146275471379047No Hit
CAGGAGGGGTTTCAAAAGTGTAAGATGCTTTGTGATTAAAGTGTGCGTGTAT840.10146275471379047No Hit
GCAGCACGGACTGAGGGTTGGCATGAATGCTTTTCTTTTTCACGTGTAACAT840.10146275471379047No Hit
TGTAAGGGGACAGTTTGGAAGGGGCAAGACCTCAACCTGTTGGGGTTTAGGG830.10025486477672155No Hit
GATATTGGGGGGGCCATATTGACAATGTCCTAGTCCTATATTTAAAAGTGCA830.10025486477672155No Hit
GTGCTAGGGAGAACAACATAATTTACACTGTGATTTATCTTTTTGCTGAGGG830.10025486477672155No Hit
TTAAATGGGCCTCCCAGCTACTGCAAGCTGGCCTCCTAAGGGGCTGTGCACC830.10025486477672155No Hit
GCCAAGGGGTGCACTGCAGATCTTCAGGAAGGTGATAAAACAATCATGGTTT830.10025486477672155No Hit
TAAAGTGGGTGCAGTGGATTTCTTGAAAAGAATCACACTTATAGTATGCCTC830.10025486477672155No Hit
AAGCAAGGGCTGTTAATACACAGAATGTGTACAGATTATTTGTTATGACAAT830.10025486477672155No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)