FastQCFastQC Report
Sat 31 Dec 2022
SRR5848186.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848186.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51030
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGCCGTCT4180.8191260043111894No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGC3840.7524985302763081No Hit
CTCTCCGGGAACTTGATTGTGGTAATCATTTCACAATATATTTGTATATCAA1140.22339800117577896No Hit
CACACAGGGTGGAAAATCTCAAAGAATCAACGTAAACTCATGGAAATAATAA1030.20184205369390557No Hit
GCATTGGGGATTGCAAGTTTCTTTGGGAGTCAGTGAGTGAATGTGAAAGCTT1010.19792279051538308No Hit
CTTGTCGGGTGAAGCATAAGGTGACACGTTTGTCTAGTTTGATTTCTGTACT980.19204389574759945No Hit
TGTACTGGGCTGGGGCTGCTCCGCGCCTCCATCGGCTACTTCCTCTTCCTCT980.19204389574759945No Hit
TAGGGGGGTAAAAAATGAAATCAAGATGAAAATTTAAAAATTCTTTAAAGTG930.18224573780129336No Hit
GAATCTGGGGACTTAAAAAACAACATGTAATAGAATGTGAGCTAATTGGGTA830.16264942190868117No Hit
GTCAATGGGTAAGGAGGTCTACTACTACTGCCTTTATTCACCATTTTTCTGT820.16068979031941993No Hit
ATATACGGGATGTGTGCATTGTTACAATGTATGTTGGATGTCTTTTGACCCT810.15873015873015872No Hit
ATGAATGGGCTATTTCCTATGCTGGAGTTTTGATTTTTAACTAAACGCAAAT800.1567705271408975No Hit
GACTTTGGGAACAAAAACCATATGATAATAGAAACAGAATAAGCATGTGATA790.1548108955516363No Hit
GCATTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC790.1548108955516363No Hit
ACACAGGCACAAAGTAAAGGGATAGGAAATATTTACCATGCATACAGAAAAC780.15285126396237508No Hit
TGCAGGGGTGGAAGATTCTCAGTCATATTCTAAAGAGTCTCTCTCTTTCCCA770.15089163237311387No Hit
TTAGCGCCGGGCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACA760.14893200078385263No Hit
TTGAAAGGGGTTGCATTAAACCTCACTGAAACCTGCTCCGTGCCCGGATGTT760.14893200078385263No Hit
GGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG750.14697236919459142No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCC740.1450127376053302No Hit
TCATGAGGGCAAAACCACTACCTAATTTCTCTATAGCATGTGGAATTTAACA740.1450127376053302No Hit
TATCATGGGTACAGTTTGTATTGTATTGCAAAAACTGCTTGGATCATGTAAT740.1450127376053302No Hit
TCATCAGTTTAAAACAATAGGCTATAAGATGTTATTTGCAAGCCTCATGGTA740.1450127376053302No Hit
GTTCAGGGGAAGAATGGAAAGAAGTAGATGCATTTGAATGATACTCAAAAGG730.143053106016069No Hit
CTAAAAGGGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACC730.143053106016069No Hit
GCGCGGGGATGAATTCTCATGCATAATTTGATTTTAATTAAATCATAGATAA730.143053106016069No Hit
CTGGCTGCCCTGCAGAAGATGTGATGAATTATCCTGTAGTACAAGATTATCT720.14109347442680778No Hit
AATAGCGGGGTATGTGCCCCGCGCATTTCCTTGGCCTCATCTGAGATCTGGT720.14109347442680778No Hit
GCAAATGGGGAGGATAATGAAAAGTCCGTGTTTTGAAGTTTTCAGATTATTT700.1371742112482853No Hit
AGCTTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA700.1371742112482853No Hit
TGTTAGAGGGCTTGTAACAGTTAATGACGCACTGACGTTGCTCTTTTCCACC700.1371742112482853No Hit
TATATAGGGAGTTAACAGAAAGGAACTGATCGTTTGTACCAGTAAAAGAGAG690.1352145796590241No Hit
AACCTGGGCCTTGTAGCTTTATTCCTTGCATCTTTGGGTCTACTGAGCAGTG690.1352145796590241No Hit
ACTGATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC690.1352145796590241No Hit
AGTAGGGAGATATGGTTCAAATAGGACTACTAGAGTTCATTGAACACTAAAA680.13325494806976287No Hit
ACATAAGGGGAAGTGGAGCCCATCATCCTCTCCGTGGGCGAGGAAGATGAGG680.13325494806976287No Hit
TAGTTGGGGCAATTGCTTTTTTGCACAATTAGGTTAATGAAAAATTTAATGA680.13325494806976287No Hit
CAGGCTGGGGAACCAATTTTTTTAATGTTCCTGTGCAATTAGTACTTTTTAC680.13325494806976287No Hit
TACAATGGGATGTAAGAATTGAAGTATTACTAAGGACAACCTCATGATGGTG680.13325494806976287No Hit
ATGAGTGGGCGGATGGTCAAGTTATCTGACCATCTCCCCTCCATAAACCTTT680.13325494806976287No Hit
TTTTATGGGCAGGAACAAAAGCAACCAATTTTTAACTTTCTCTTCTCATTCC680.13325494806976287No Hit
TTACTTGGGGAAACAGGTAATGAATAAGGAAAAATGGATAAAAACCAGTGGA670.13129531648050166No Hit
TATGAAGGGGCCAGAAAGGAAGTGGGCATGTCGTAAGAAGACTTCAGTGAAG670.13129531648050166No Hit
TTACATGGGTCAGGTAGAGGAACTCTGCCATGAACACCTCTGGAATCGTGTC670.13129531648050166No Hit
CGACATGGGAACTATGCTGGTGCCAAATTTAGTGAGCCGCCATCACCAAGTG670.13129531648050166No Hit
GTTACAGGGGTCTGACAGAGGGGGAAACCACTGCAGGTATTTTAAGTAAAAG670.13129531648050166No Hit
CACCAGGGGAATATATAAACATTCAAACATTTTGCTATTCATATTTCATCTA660.12933568489124045No Hit
GGGGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC640.125416421712718No Hit
AACCAGGGGATGACGCTCAATTTTAATTCCTTCTGTTACTCATGACATTCAC640.125416421712718No Hit
TTAGCTGGGATTGCTAAATAGAGAACCACACTTTATATAAAGTAGTTTTTGC640.125416421712718No Hit
ATCGTAGGGACAGTTTACCTAATTATTCCTGTTAGGCAACGTCTGATATTTT640.125416421712718No Hit
AACGACGGGCTGCTATACTGTCAGAGGACTATACGGCTTTCTGTGGATTCAG630.12345679012345678No Hit
CAGATGGGGACTATCTCTGATGTCAATGTGAATTTTTTTCTTCTTTTAAATT630.12345679012345678No Hit
TCTATCGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA620.12149715853419557No Hit
GTACTTGGGATGCAGAGGTCAAATTAGTATATTGTCAACCTCATTTTTTTTT610.11953752694493436No Hit
AATCTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA610.11953752694493436No Hit
TATTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC600.11757789535567313No Hit
AGTAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA600.11757789535567313No Hit
TTTACTGGGGTTTTAGATTTCCAGAGATGTTTAATAATGATTTCCATTATTC600.11757789535567313No Hit
CGGATAGGGAGGGACATTTGCCCTGACATTTCTCAAAGACTGCTTAGGTTTT600.11757789535567313No Hit
CAATCTGGGATATAAATGAATATTTGAATTTTTCTTTTTAGAATTCAAATTC600.11757789535567313No Hit
CAACTGGGGGGAGACAGGTGGGGTACTCGGGAAGCTGGAGCGGGCCGGCGGT600.11757789535567313No Hit
ATTACGGGGGGCCTGGAGCCGCCGGGAGTGGACGCCGCCGAGGCCCGGAGTC600.11757789535567313No Hit
ATGAATGGGGGCTACCTAACTAGTATAGCATTATAGAATTTATCATTTCTGA600.11757789535567313No Hit
ACGTTGGGGAGTGGAAGAGGAGGAATGAAAGATGCAAACTTTAAGGTTTCAG600.11757789535567313No Hit
GTTTGCGGGATGTATGTTTATCTTACCCTACAGCATATAGAGAAGAAAATGG600.11757789535567313No Hit
TTAGCAGGGTAGAGGCGGGATGACCCCAATACGCAAAACTAACCCCCTAATA590.11561826376641192No Hit
TTTCTGGGGGAAATAAGGTAATCTGAATAGTCCTATCACTATTAAGGAGACG590.11561826376641192No Hit
TGATCAGGGACCTGTTGTGTGAGTTTAGTATATTTAATTTACTTTTTGTTAC590.11561826376641192No Hit
CATGAAGGGCTGCGAGAGTTGGTAGAACCTCTCCATGCCAAATCGGATCCAC590.11561826376641192No Hit
CTTAAAGGGGCTCTTCCGACAGTTGTGTTGTGCCAATGGTGGAGAAGAAAAC580.11365863217715069No Hit
ATTTATGGGGAGAAAGTGTGTGTTTGTGGTGGGTGGTTTTTAAACTATATAT580.11365863217715069No Hit
CCTGGAGGGATCTTGCCGTGGGACTGAGTCCGGAGGTCATCCCTGAGCTCAT580.11365863217715069No Hit
GAAATGGGGCTGGGTAGTAGGAGTAAAAGGAGGAAACGATCTGAAGAGACCT580.11365863217715069No Hit
CCTTTTGGGGTGAGCAAGATATGTCAAAAACCAAAATAGGCAAAAGCTAGAC580.11365863217715069No Hit
GTGCAAGGGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAAT570.11169900058788948No Hit
TCGACAGGGGAGACGCCTCTGCGGCAGCTGGTGGCGCAGGTGGCTTGCGTGG570.11169900058788948No Hit
TTGCTAGGGACAACCGTCATCTAAGGTTGTCTCTCATTTCCATCCCCCTCAA570.11169900058788948No Hit
TTTTCCGGGGGAGCTGGCAGTGAACCGAGATCGCGCCACTGCACTCCATCCT570.11169900058788948No Hit
AAGCTTGGGTAAGAGTTTTGAACATGCTATTACACATTGTGAATTTATAAAA570.11169900058788948No Hit
TATCTTGGGCAGAGTGGAGAGGGACTGAAGATTGAGCTGGTCACCAATAGCC570.11169900058788948No Hit
AGCGCAGGGCAATACCATCCTCACTAGAAGACTGTCAGCAGGTATTTTCATC570.11169900058788948No Hit
AGTGCAGGGATTCTGGGTCCTAGATTACTGTCATTGTCTAAAAGTAACTTTT570.11169900058788948No Hit
CTCTAGGGGTTTTCTAGACATGTAACTCTCTGGGGAGTATAAAACTGGTCAC570.11169900058788948No Hit
GTATGTGGGCATTTTGCCATAAGGGAGAAAGTGATGTTCTCTGAGAAGACAC560.10973936899862827No Hit
GTGGCGGGTGTTACTTGAAATTTTATTGTTCCCTGAGTGTTGACTTCAAAAT560.10973936899862827No Hit
GTCTATGGGGTTTCTTTATTCCTAATGCAGTTAGAAAGACCTTTCTCCTTGA560.10973936899862827No Hit
AATGCAGGGTGAAACATGAACATCATTGGTCCATGCATGACCCATTTTACTA560.10973936899862827No Hit
CATTTAGGGTATGAAGTTTTCTCAACATAAATCTGCTATAATTAAAAATTAC560.10973936899862827No Hit
GTTCCCGGGAAGACAGACCAAGTGTATGCTCACGGAGCTGAAATGCATTAGT560.10973936899862827No Hit
GCTGCAGGGAGCAAGAGCCCATCTCTAAAACAAAACAAAACAAAACAAAACA560.10973936899862827No Hit
CCATCTGGGGAAATAAATTCTGAGCTGCTGCTTTTGATATAGTCAGACTTAA550.10777973740936704No Hit
TTTGCTGGGCCACAATGACCTAGAGTGCATCCTGCTCATTGTCAGTGTAGCC550.10777973740936704No Hit
TAGCGCGGGGCTACCTTATAAGCTATGGGGAGTTTGGTAACGTAACTAGATG550.10777973740936704No Hit
AAATAAGGGCAATTATCTGGTCTTTTCCTGTTGTGCAAAAATGACTCATTGC550.10777973740936704No Hit
CATAGAGGGCATTATTAGAATCAGATTTGCATGTTGCATTCATTGTTCTCAC550.10777973740936704No Hit
TGGCATGGCTTGTTAGGGCTTGTACCTTGGGCAAGTTACTAATCTTTTTTCT550.10777973740936704No Hit
TGCCAGGGGCTGACATTAGTGCACATAATGCAGAGTTTAACCTTGATTCTTC550.10777973740936704No Hit
CTACTAGGGGAGTTCCTTATATATTCTGGTTATGAATCCCTTGTTAGATGGT540.10582010582010583No Hit
ATTCTTGGGGTCTTTCTGGCTTGTTAGATCTTCTCATTTGGCGTCCTTTCTC540.10582010582010583No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGT540.10582010582010583No Hit
ATTACAGGGGGAGAATAATGAAAAGTCCGTGTTTTGAAGTTTTCAGATTATT540.10582010582010583No Hit
TGTGTTGGGGGAAGGAGACTTGGGGGCACCCGCGTCGTGCCTCCTGGGTTGT540.10582010582010583No Hit
TCAGCAGGGATTGGGGAGCATTCTGATGCAGCAGAGCTTGGAAGGATGCTTC540.10582010582010583No Hit
AAAACGGGGAACCGGGCCGGCTTCAGTGTCGCCGCCATCTTTGTTGATGTGT540.10582010582010583No Hit
AAAAAAGGGAGAAATAGCCACTGCTAACATGGTGATTGGTATCCTTTGGGTC540.10582010582010583No Hit
AGGGCTGGGGCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGG540.10582010582010583No Hit
TTGGCCGGGAACCATAATAACAAGTCTTGTCTTACCCTAAAGTCAAATGCTG540.10582010582010583No Hit
GTCTTTGGGGCATATGCCCAAATTGTTCAGTTTGGCATGAATGAAAAGGTTG540.10582010582010583No Hit
GGCAACGGGGAAGAGGAAGCCAAAAAAGTATATCAAAATGTGATTAAAAGAT530.10386047423084462No Hit
AACAGTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC530.10386047423084462No Hit
CATCCTGGCCCTCCACAGGAAGATCAAGAATGAGCCCGTGGTGTTTCCTGAG530.10386047423084462No Hit
AAGTAGGGGACTGAGTGCCTCTCTGCCAGAAAGACCCAGCAATCCCCAAACG530.10386047423084462No Hit
GATCGAGGGAATGTTACAAAAGAAATTGAGAAAGCAAAGTCTCAGTTTGAAG530.10386047423084462No Hit
ACACTTGGGAAGTCCTTGGGAGAGGCTGGGAGCAGTCACTGCGCGGCTTGCG530.10386047423084462No Hit
TAATGGGGGAGTGTCAAACTTTGGGAGTAAAATCTTTCATGTTTTTCTGAAG530.10386047423084462No Hit
TCGCATGGGAAGGAAACTTACAAACTGCAACGCAGAGAAACTATTAAAGGTA530.10386047423084462No Hit
CTTTCAGGGCGATCTCTTGACCTCATGATCCACCCGCCTCGGCCTCCAAAAG530.10386047423084462No Hit
ATATCAGGGAAGCTACACTAGCAGAAAAGTGAGTGAAAATAAAATGTAGAAA530.10386047423084462No Hit
AAGCAAGGGAGAGCATGGGTGGCAGTCTTACACGATGAGACAACGGGTGTCT530.10386047423084462No Hit
CGTCAGGGGGAAACCAAGTACCATAGACAGGGTGGCTTATAAACAACATAAA520.10190084264158339No Hit
TAATTGGGGTGGCTATTTGGGGTTCCTTGAGATTGCATTTGAATTTTATGGG520.10190084264158339No Hit
GCATAGGGGATTTTGGTGGCTAATCCTTCATAGACATTGTTGGCAAACTAAT520.10190084264158339No Hit
TATGACGGGGACTTTTTTTAATGAAAAAATGTTTTTTGGTTTTAATGGTGTT520.10190084264158339No Hit
AGAAATGGGCTGTCTTTTCTTGGCCTAAATTATTTCAGAGCTAGCACAAAAA520.10190084264158339No Hit
TGAACCGGGATTGTCTTGCTCATTATAGAGAGTTAAGACTGTGCATAAAATA520.10190084264158339No Hit
TAGCAGGGGAGTCTGAGGTGCGGGGTCCTGGGGCCCGGCGCGGGTGGCCGCC520.10190084264158339No Hit
CAACCAGGGTGGATGTCCCCACCTTCTTAGAGTTTGCAATTGTCATATTTAA520.10190084264158339No Hit
TCCCATGGGCATGTCTTCTGACATTCCTGGTGTCCCAAAGAATAGCAAAAAG520.10190084264158339No Hit
AATACTGGGATAGCGTGGATTCCCCGGAACAGCTGTCTGGAATAAGGATGAT520.10190084264158339No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)