FastQCFastQC Report
Sat 31 Dec 2022
SRR5848185.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848185.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51526
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGCCGTCT4280.830648604587975No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGC3820.741373287272445No Hit
GCATTGGGGATTGCAAGTTTCTTTGGGAGTCAGTGAGTGAATGTGAAAGCTT1210.23483289989519857No Hit
CTCTCCGGGAACTTGATTGTGGTAATCATTTCACAATATATTTGTATATCAA1070.20766215114699374No Hit
GCATTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1020.19795831230834918No Hit
CTTGTCGGGTGAAGCATAAGGTGACACGTTTGTCTAGTTTGATTTCTGTACT1000.19407677677289137No Hit
AATAGCGGGGTATGTGCCCCGCGCATTTCCTTGGCCTCATCTGAGATCTGGT920.17855063463106005No Hit
CACCAGGGGAATATATAAACATTCAAACATTTTGCTATTCATATTTCATCTA910.17660986686333113No Hit
ACACAGGCACAAAGTAAAGGGATAGGAAATATTTACCATGCATACAGAAAAC910.17660986686333113No Hit
TATGAAGGGGCCAGAAAGGAAGTGGGCATGTCGTAAGAAGACTTCAGTGAAG900.17466909909560221No Hit
AGCTTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA890.1727283313278733No Hit
AACGACGGGCTGCTATACTGTCAGAGGACTATACGGCTTTCTGTGGATTCAG830.16108372472149982No Hit
CAGGCTGGGGAACCAATTTTTTTAATGTTCCTGTGCAATTAGTACTTTTTAC830.16108372472149982No Hit
TTGAAAGGGGTTGCATTAAACCTCACTGAAACCTGCTCCGTGCCCGGATGTT830.16108372472149982No Hit
TAGGGGGGTAAAAAATGAAATCAAGATGAAAATTTAAAAATTCTTTAAAGTG820.15914295695377093No Hit
ATTACGGGGGGCCTGGAGCCGCCGGGAGTGGACGCCGCCGAGGCCCGGAGTC810.157202189186042No Hit
TTGGCCGGGAACCATAATAACAAGTCTTGTCTTACCCTAAAGTCAAATGCTG810.157202189186042No Hit
GGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG800.1552614214183131No Hit
ACACAAGGGAGACTTAAACTCTACACATCAGTTTTGAAACCTAGGTCTCCCT800.1552614214183131No Hit
GTTCATGGGACAGAGGTGAGGTGGCTTTCTCGAGGTAAAGTTCTTGTAAGAC790.15332065365058417No Hit
ATGAGTGGGCGGATGGTCAAGTTATCTGACCATCTCCCCTCCATAAACCTTT790.15332065365058417No Hit
TCGACAGGGGAGACGCCTCTGCGGCAGCTGGTGGCGCAGGTGGCTTGCGTGG780.15137988588285525No Hit
GAATCTGGGGACTTAAAAAACAACATGTAATAGAATGTGAGCTAATTGGGTA760.14749835034739744No Hit
TGTACTGGGCTGGGGCTGCTCCGCGCCTCCATCGGCTACTTCCTCTTCCTCT760.14749835034739744No Hit
ATGAATGGGGGCTACCTAACTAGTATAGCATTATAGAATTTATCATTTCTGA760.14749835034739744No Hit
CTAAAAGGGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACC760.14749835034739744No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCC750.1455575825796685No Hit
GTCCACGGGATGAGTGGAAGCTACATGAGTGTTTTAAATATTTCATAATTTT730.1416760470442107No Hit
TTACATGGGTCAGGTAGAGGAACTCTGCCATGAACACCTCTGGAATCGTGTC730.1416760470442107No Hit
TGTTAGAGGGCTTGTAACAGTTAATGACGCACTGACGTTGCTCTTTTCCACC730.1416760470442107No Hit
GTATGAGGGAGGGCATGGGGCACACCTGCTGCTCAGGGTTGCCCCTTTCCAG720.13973527927648177No Hit
GACTTTGGGAACAAAAACCATATGATAATAGAAACAGAATAAGCATGTGATA720.13973527927648177No Hit
CGACATGGGAACTATGCTGGTGCCAAATTTAGTGAGCCGCCATCACCAAGTG720.13973527927648177No Hit
GTCAATGGGTAAGGAGGTCTACTACTACTGCCTTTATTCACCATTTTTCTGT720.13973527927648177No Hit
CCTTTTGGGGTGAGCAAGATATGTCAAAAACCAAAATAGGCAAAAGCTAGAC720.13973527927648177No Hit
CACACAGGGTGGAAAATCTCAAAGAATCAACGTAAACTCATGGAAATAATAA710.13779451150875288No Hit
TTTACTGGGGTTTTAGATTTCCAGAGATGTTTAATAATGATTTCCATTATTC700.13585374374102394No Hit
TGCAGGGGTGGAAGATTCTCAGTCATATTCTAAAGAGTCTCTCTCTTTCCCA700.13585374374102394No Hit
ACGTTGGGGAGTGGAAGAGGAGGAATGAAAGATGCAAACTTTAAGGTTTCAG700.13585374374102394No Hit
ATTTATGGGGAGAAAGTGTGTGTTTGTGGTGGGTGGTTTTTAAACTATATAT690.13391297597329502No Hit
TCATGAGGGCAAAACCACTACCTAATTTCTCTATAGCATGTGGAATTTAACA690.13391297597329502No Hit
ATATACGGGATGTGTGCATTGTTACAATGTATGTTGGATGTCTTTTGACCCT680.13197220820556613No Hit
AACCTGGGCCTTGTAGCTTTATTCCTTGCATCTTTGGGTCTACTGAGCAGTG680.13197220820556613No Hit
AAAAATGGGGATGCTCTTCAGCATTTGCCAGGGAGTCATCATTCTGAAAACC680.13197220820556613No Hit
GTTCCCGGGAAGACAGACCAAGTGTATGCTCACGGAGCTGAAATGCATTAGT680.13197220820556613No Hit
TCATCAGTTTAAAACAATAGGCTATAAGATGTTATTTGCAAGCCTCATGGTA680.13197220820556613No Hit
GTGCAAGGGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAAT670.1300314404378372No Hit
ATGAATGGGCTATTTCCTATGCTGGAGTTTTGATTTTTAACTAAACGCAAAT660.12809067267010832No Hit
GAGGCAGGGAATAGATGGATGGCAGACATTAACTGCGTGCCTGGCCCTATGA660.12809067267010832No Hit
CGGCACGGGTGCTGAGTCTGCAGGAGTTCTCCAACATGGACCTTCGGGACTT660.12809067267010832No Hit
TATTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC650.12614990490237937No Hit
AACTCGGGATCGAGGTGCAGGGGGACCAGCAGGCATGGTTTGGTTTCGCTGG650.12614990490237937No Hit
ACCGTGGGGTGAATGCCTGGCCCTAAACCTGCACTGCTTGGGGGGACAGCAG650.12614990490237937No Hit
TTAGCAGGGTAGAGGCGGGATGACCCCAATACGCAAAACTAACCCCCTAATA650.12614990490237937No Hit
AGCGCAGGGCAATACCATCCTCACTAGAAGACTGTCAGCAGGTATTTTCATC650.12614990490237937No Hit
ACACATGGGTGAGTGATTTTAAAATGTTTTGGTGAATGTGAAAACTAAAGTT650.12614990490237937No Hit
ACTGATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC650.12614990490237937No Hit
CGCGTGGGGTCAGTTATAGAACAAGTTCATTTTGCATGAACTTCTCTTATTG640.12420913713465045No Hit
TTACTTGGGGAAACAGGTAATGAATAAGGAAAAATGGATAAAAACCAGTGGA640.12420913713465045No Hit
GTTCAGGGGAAGAATGGAAAGAAGTAGATGCATTTGAATGATACTCAAAAGG640.12420913713465045No Hit
ATTACAGGGGGAGAATAATGAAAAGTCCGTGTTTTGAAGTTTTCAGATTATT640.12420913713465045No Hit
TATATAGGGAGTTAACAGAAAGGAACTGATCGTTTGTACCAGTAAAAGAGAG640.12420913713465045No Hit
AACCAGGGGATGACGCTCAATTTTAATTCCTTCTGTTACTCATGACATTCAC630.12226836936692156No Hit
TCTGGTGGGACCACAGGATGCATATTAAAGAAAGTCATAGTTTTTTTCTTTT630.12226836936692156No Hit
TACAATGGGATGTAAGAATTGAAGTATTACTAAGGACAACCTCATGATGGTG630.12226836936692156No Hit
CACGCTGGGATTAAATTCAGCTGATCATATTGATCAGTAGATAAACGTAAAT630.12226836936692156No Hit
TCTCCAGGGGAAGAGAACCAACTAAAAGAGCTTCCCAGATGTCCAATAGATG630.12226836936692156No Hit
GCGCGGGGATGAATTCTCATGCATAATTTGATTTTAATTAAATCATAGATAA630.12226836936692156No Hit
AGTAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA620.12032760159919265No Hit
ACATAAGGGGAAGTGGAGCCCATCATCCTCTCCGTGGGCGAGGAAGATGAGG620.12032760159919265No Hit
CTACTAGGGGAGTTCCTTATATATTCTGGTTATGAATCCCTTGTTAGATGGT610.11838683383146371No Hit
CACAAAGGGATATGTTTACTGGTGACAGCGTGAAAGAGCAAGTAGAGAAGAT610.11838683383146371No Hit
TATCATGGGTACAGTTTGTATTGTATTGCAAAAACTGCTTGGATCATGTAAT610.11838683383146371No Hit
AGATCAGGGGTGAAACCTTTTTTAAGATTTTTCTCAAAGTTTTGAAAAGCTA610.11838683383146371No Hit
TTTACAGGGGTCTGATTGCATGTCTTCTAAAGTATGCAGTACAGCTAACAAC610.11838683383146371No Hit
TCCCATGGGCATGTCTTCTGACATTCCTGGTGTCCCAAAGAATAGCAAAAAG610.11838683383146371No Hit
AACAGTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC600.11644606606373482No Hit
AGAACAGGGCCCAAATGCATTTATTGAATGCCTTAGACATATGTATTTATTA600.11644606606373482No Hit
CTAACAGTGGGCAATTGCAAATGATAAGCATTTTTGTGAGTGACCACCTTTG600.11644606606373482No Hit
GTCCAGGGAAGGATTTAAAGGGGTATTTGTAAACTTTGCCCTCCTTGAGAAA600.11644606606373482No Hit
CAGATGGGGACTATCTCTGATGTCAATGTGAATTTTTTTCTTCTTTTAAATT600.11644606606373482No Hit
CTCAATGGGAGAGAAGGGAAGGCAAAGCGCATGGGGGTAGTGTGGGATGGTC590.11450529829600589No Hit
CCATCTGGGGAAATAAATTCTGAGCTGCTGCTTTTGATATAGTCAGACTTAA590.11450529829600589No Hit
TTTCTGGGGGAAATAAGGTAATCTGAATAGTCCTATCACTATTAAGGAGACG590.11450529829600589No Hit
TGAACCGGGATTGTCTTGCTCATTATAGAGAGTTAAGACTGTGCATAAAATA590.11450529829600589No Hit
GCTGCAGGGAGCAAGAGCCCATCTCTAAAACAAAACAAAACAAAACAAAACA590.11450529829600589No Hit
AGCCGGGGTGCGAGAATTCATGTTTTACCAGTCATTGTTATATTACAAACAG590.11450529829600589No Hit
AATACTGGGATAGCGTGGATTCCCCGGAACAGCTGTCTGGAATAAGGATGAT590.11450529829600589No Hit
ACTTGCGGGGCTCTTCCGGTCGCGGGACACCGGGTGTAGAGGGCGGTCGCGG590.11450529829600589No Hit
ATTCAGGGACTCTGCTTGGATTCCCCTCTTATACAATGGTCTACCAAGTGAC580.112564530528277No Hit
CACTGCGGGATGCCCTAGAGTCCTTCACTGGGTTGACCATGCCATTGATCAT580.112564530528277No Hit
TTAAAGGGGGTGCTGCTGGTGCCTCTTTCCAAAGGACGTTGAATTGCTTGAC580.112564530528277No Hit
GAAATGGGGCTGGGTAGTAGGAGTAAAAGGAGGAAACGATCTGAAGAGACCT580.112564530528277No Hit
GTTTGCGGGATGTATGTTTATCTTACCCTACAGCATATAGAGAAGAAAATGG580.112564530528277No Hit
GGGGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC570.11062376276054807No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGT570.11062376276054807No Hit
CGTTCAGGGCATACATTCTACGAAAAGTGCCAGACTAAATATAAAATTACAT570.11062376276054807No Hit
TGCGGCCGGGTGATGAGGCTTATGGCCAAATTGGGCAGTTTCTGCTTTTTCT570.11062376276054807No Hit
TAGCGCGGGGCTACCTTATAAGCTATGGGGAGTTTGGTAACGTAACTAGATG570.11062376276054807No Hit
GGGCAAGGGTCTGGGAGTATAGACTTAGGGTTTATCTATTTTTTTTTTTTTT570.11062376276054807No Hit
ATCTGCGGGCCCTGCCTCCCACCCAGGTCCACCTGCCACCTGGTGACCACCT560.10868299499281915No Hit
CCTGGAGGGATCTTGCCGTGGGACTGAGTCCGGAGGTCATCCCTGAGCTCAT560.10868299499281915No Hit
GTCTTTGGGGCATATGCCCAAATTGTTCAGTTTGGCATGAATGAAAAGGTTG560.10868299499281915No Hit
TGTCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC550.10674222722509025No Hit
TTGCTAGGGACAACCGTCATCTAAGGTTGTCTCTCATTTCCATCCCCCTCAA550.10674222722509025No Hit
CTGAACGGGGTGGGTTCACCATGATACTTAGGGAGAGACTTGATTAAATGAA550.10674222722509025No Hit
CTATGCGGGAACTATATTTGTGTGTGTGTGTGTTTCTGTGAGGGATAGTTTT550.10674222722509025No Hit
GTACTTGGGATGCAGAGGTCAAATTAGTATATTGTCAACCTCATTTTTTTTT550.10674222722509025No Hit
CAGTAGGGGAGTCATTCCTTATGAGAAAAAAAACGGACCACCGTCTGTTGAA550.10674222722509025No Hit
CATGAAGGGCTGCGAGAGTTGGTAGAACCTCTCCATGCCAAATCGGATCCAC550.10674222722509025No Hit
ATCGTAGGGACAGTTTACCTAATTATTCCTGTTAGGCAACGTCTGATATTTT550.10674222722509025No Hit
CTTGTTGGGGCACCCACAAATAAATGACAAGTGAGACAAAAAGCACTAGTAT550.10674222722509025No Hit
GTAGCCGGGTGCTAGTTTTGGTTCGGTTTTGCAATGGTTCTGTTGTCAGGAA550.10674222722509025No Hit
CGTCAGGGGGAAACCAAGTACCATAGACAGGGTGGCTTATAAACAACATAAA540.10480145945736133No Hit
TTTTCCGGGGGAGCTGGCAGTGAACCGAGATCGCGCCACTGCACTCCATCCT540.10480145945736133No Hit
AAGCTTGGGTAAGAGTTTTGAACATGCTATTACACATTGTGAATTTATAAAA540.10480145945736133No Hit
CTGGCTGCCCTGCAGAAGATGTGATGAATTATCCTGTAGTACAAGATTATCT540.10480145945736133No Hit
TTAGCTGGGATTGCTAAATAGAGAACCACACTTTATATAAAGTAGTTTTTGC540.10480145945736133No Hit
CGTAAGGGTGAGTGCAGACAAATAAAAGCCGGCTTTGTGGGTGTTGATTGAA540.10480145945736133No Hit
CAACCAGGGTGGATGTCCCCACCTTCTTAGAGTTTGCAATTGTCATATTTAA540.10480145945736133No Hit
TATTTCTGGGTTGCTGCATCAAATGTAAACGACGAACAAGATGATGAAGCCG540.10480145945736133No Hit
GCCACTGCATTTCATATCACACTGTTAAAAGTTGTGTTTTGAAATTTTATGT540.10480145945736133No Hit
TTTTATGGGCAGGAACAAAAGCAACCAATTTTTAACTTTCTCTTCTCATTCC540.10480145945736133No Hit
GGATCTGGGCAAATGTTTCCTGAGTTGTATGAAGCTAAGATACAGGTAAAAA530.10286069168963241No Hit
GTTATGGGGAGTTCTATTTCAACATGTGGGTTCACAGATTTATTCTAACCTT530.10286069168963241No Hit
GTCAATGGGAATGAAATAGTGACCAATTTCTGAAAATGTTTTTCTGTAATAT530.10286069168963241No Hit
TGTTAGGGGATATAAAAGCTTTCTGAGAGATTATTTACTATATGAATATATT530.10286069168963241No Hit
CTGTTAGGGGATTCCAGGACCTTAACCTCTGCCTGCCTTCGCGGCTCTCAGC530.10286069168963241No Hit
TTTAAAGGGATGTCACTTACGGAAGCGAGGCATTGGGTATATGAGTAGGCAC530.10286069168963241No Hit
AGCGCGGGGCTCTTGGGCAGAATCCCACAGAAGCAGAGTTACAGGACATGAT530.10286069168963241No Hit
AATCTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA530.10286069168963241No Hit
GCTAGGGTACTCCCATCATGATTTCTTGTGAATATTTGTTATATGTCTTCTG530.10286069168963241No Hit
TGACCCGGGTAAATGAGTTCATTTTGTTCACTTTCCAATTTTCCCGAGTATC520.1009199239219035No Hit
GACAAAGGGGTGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC520.1009199239219035No Hit
ACTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA520.1009199239219035No Hit
CAATCTGGGATATAAATGAATATTTGAATTTTTCTTTTTAGAATTCAAATTC520.1009199239219035No Hit
CTAGCGATGGGATAGTATTCTCTTCACTAAAGGTAATATGTTGTCATGTTTA520.1009199239219035No Hit
TTAGCGCCGGGCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACA520.1009199239219035No Hit
TAGCAGGGGAGTCTGAGGTGCGGGGTCCTGGGGCCCGGCGCGGGTGGCCGCC520.1009199239219035No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)