FastQCFastQC Report
Sat 31 Dec 2022
SRR5848184.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848184.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50653
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGCCGTCT4680.9239334294118807No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGC3820.7541507906738002No Hit
AATAGCGGGGTATGTGCCCCGCGCATTTCCTTGGCCTCATCTGAGATCTGGT1050.20729275659881943No Hit
CTTGTCGGGTGAAGCATAAGGTGACACGTTTGTCTAGTTTGATTTCTGTACT1010.19939588968076916No Hit
GCATTGGGGATTGCAAGTTTCTTTGGGAGTCAGTGAGTGAATGTGAAAGCTT980.19347323949223147No Hit
TGTACTGGGCTGGGGCTGCTCCGCGCCTCCATCGGCTACTTCCTCTTCCTCT960.18952480603320634No Hit
ACACAGGCACAAAGTAAAGGGATAGGAAATATTTACCATGCATACAGAAAAC920.18162793911515607No Hit
AGCTTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA920.18162793911515607No Hit
CACCAGGGGAATATATAAACATTCAAACATTTTGCTATTCATATTTCATCTA910.17965372238564348No Hit
GACTTTGGGAACAAAAACCATATGATAATAGAAACAGAATAAGCATGTGATA890.17570528892661838No Hit
CTCTCCGGGAACTTGATTGTGGTAATCATTTCACAATATATTTGTATATCAA870.17175685546759323No Hit
AGTAGGGAGATATGGTTCAAATAGGACTACTAGAGTTCATTGAACACTAAAA860.16978263873808067No Hit
TTTCTGGGGGAAATAAGGTAATCTGAATAGTCCTATCACTATTAAGGAGACG860.16978263873808067No Hit
TATGAAGGGGCCAGAAAGGAAGTGGGCATGTCGTAAGAAGACTTCAGTGAAG850.1678084220085681No Hit
TTACATGGGTCAGGTAGAGGAACTCTGCCATGAACACCTCTGGAATCGTGTC820.1618857718200304No Hit
CAGGCTGGGGAACCAATTTTTTTAATGTTCCTGTGCAATTAGTACTTTTTAC820.1618857718200304No Hit
ATGAATGGGCTATTTCCTATGCTGGAGTTTTGATTTTTAACTAAACGCAAAT810.15991155509051783No Hit
CTAAAAGGGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACC780.15398890490198014No Hit
ACACAAGGGAGACTTAAACTCTACACATCAGTTTTGAAACCTAGGTCTCCCT780.15398890490198014No Hit
GTCCACGGGATGAGTGGAAGCTACATGAGTGTTTTAAATATTTCATAATTTT770.15201468817246758No Hit
GCATTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC760.15004047144295501No Hit
ACGTTGGGGAGTGGAAGAGGAGGAATGAAAGATGCAAACTTTAAGGTTTCAG760.15004047144295501No Hit
TTTTATGGGCAGGAACAAAAGCAACCAATTTTTAACTTTCTCTTCTCATTCC750.14806625471344242No Hit
GGATCTGGGCAAATGTTTCCTGAGTTGTATGAAGCTAAGATACAGGTAAAAA730.1441178212544173No Hit
CATGAAGGGCTGCGAGAGTTGGTAGAACCTCTCCATGCCAAATCGGATCCAC720.14214360452490474No Hit
TCATCAGTTTAAAACAATAGGCTATAAGATGTTATTTGCAAGCCTCATGGTA710.14016938779539218No Hit
GGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG710.14016938779539218No Hit
GTGCAAGGGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAAT700.1381951710658796No Hit
AACTCGGGATCGAGGTGCAGGGGGACCAGCAGGCATGGTTTGGTTTCGCTGG700.1381951710658796No Hit
GAATCTGGGGACTTAAAAAACAACATGTAATAGAATGTGAGCTAATTGGGTA700.1381951710658796No Hit
TTTACTGGGGTTTTAGATTTCCAGAGATGTTTAATAATGATTTCCATTATTC700.1381951710658796No Hit
TGTTAGGGGATATAAAAGCTTTCTGAGAGATTATTTACTATATGAATATATT690.13622095433636705No Hit
CGACATGGGAACTATGCTGGTGCCAAATTTAGTGAGCCGCCATCACCAAGTG690.13622095433636705No Hit
TTACTTGGGGAAACAGGTAATGAATAAGGAAAAATGGATAAAAACCAGTGGA680.1342467376068545No Hit
CACACAGGGTGGAAAATCTCAAAGAATCAACGTAAACTCATGGAAATAATAA680.1342467376068545No Hit
ATCTGCGGGCCCTGCCTCCCACCCAGGTCCACCTGCCACCTGGTGACCACCT680.1342467376068545No Hit
ATATACGGGATGTGTGCATTGTTACAATGTATGTTGGATGTCTTTTGACCCT680.1342467376068545No Hit
TGAACCGGGATTGTCTTGCTCATTATAGAGAGTTAAGACTGTGCATAAAATA670.13227252087734193No Hit
CTGTTAGGGGATTCCAGGACCTTAACCTCTGCCTGCCTTCGCGGCTCTCAGC670.13227252087734193No Hit
TATTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC650.12832408741831677No Hit
TTTTCCGGGGGAGCTGGCAGTGAACCGAGATCGCGCCACTGCACTCCATCCT650.12832408741831677No Hit
TATCATGGGTACAGTTTGTATTGTATTGCAAAAACTGCTTGGATCATGTAAT650.12832408741831677No Hit
GTTTGCGGGATGTATGTTTATCTTACCCTACAGCATATAGAGAAGAAAATGG650.12832408741831677No Hit
CAATCTGGGATATAAATGAATATTTGAATTTTTCTTTTTAGAATTCAAATTC640.1263498706888042No Hit
GCTGCAGGGAGCAAGAGCCCATCTCTAAAACAAAACAAAACAAAACAAAACA640.1263498706888042No Hit
AGCCGGGGTGCGAGAATTCATGTTTTACCAGTCATTGTTATATTACAAACAG640.1263498706888042No Hit
GTCAATGGGTAAGGAGGTCTACTACTACTGCCTTTATTCACCATTTTTCTGT640.1263498706888042No Hit
TAGGGGGGTAAAAAATGAAATCAAGATGAAAATTTAAAAATTCTTTAAAGTG640.1263498706888042No Hit
ATGCCTGGGATAGTATGATTCTAAGCCTATTCATTAAGGAACATTAGAGTTT630.12437565395929165No Hit
AACGACGGGCTGCTATACTGTCAGAGGACTATACGGCTTTCTGTGGATTCAG630.12437565395929165No Hit
GTATGAGGGAGGGCATGGGGCACACCTGCTGCTCAGGGTTGCCCCTTTCCAG630.12437565395929165No Hit
ATCACTGGGTGGCTGAATTCTTTTAAGAGGTAGGTTTACAACCTAAGCTTAA630.12437565395929165No Hit
TCGACAGGGGAGACGCCTCTGCGGCAGCTGGTGGCGCAGGTGGCTTGCGTGG620.1224014372297791No Hit
TGTCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC620.1224014372297791No Hit
TTAGCAGGGTAGAGGCGGGATGACCCCAATACGCAAAACTAACCCCCTAATA620.1224014372297791No Hit
ATTACAGGGGGAGAATAATGAAAAGTCCGTGTTTTGAAGTTTTCAGATTATT620.1224014372297791No Hit
ATTTATGGGGAGAAAGTGTGTGTTTGTGGTGGGTGGTTTTTAAACTATATAT620.1224014372297791No Hit
AAAAATGGGGATGCTCTTCAGCATTTGCCAGGGAGTCATCATTCTGAAAACC620.1224014372297791No Hit
CATCCTGGCCCTCCACAGGAAGATCAAGAATGAGCCCGTGGTGTTTCCTGAG610.12042722050026652No Hit
AGTAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA610.12042722050026652No Hit
CTGGCTGCCCTGCAGAAGATGTGATGAATTATCCTGTAGTACAAGATTATCT610.12042722050026652No Hit
TATATAGGGAGTTAACAGAAAGGAACTGATCGTTTGTACCAGTAAAAGAGAG610.12042722050026652No Hit
TACAATGGGATGTAAGAATTGAAGTATTACTAAGGACAACCTCATGATGGTG610.12042722050026652No Hit
CTATCAGGGAAGCACACTGGAGATTTTTCTCTGCCAGTTTGGCAGGTGAAAA610.12042722050026652No Hit
TCATGAGGGCAAAACCACTACCTAATTTCTCTATAGCATGTGGAATTTAACA610.12042722050026652No Hit
TTTACAGGGGTCTGATTGCATGTCTTCTAAAGTATGCAGTACAGCTAACAAC610.12042722050026652No Hit
GCAAATGGGGAGGATAATGAAAAGTCCGTGTTTTGAAGTTTTCAGATTATTT600.11845300377075396No Hit
GTCTTTGGGGCATATGCCCAAATTGTTCAGTTTGGCATGAATGAAAAGGTTG600.11845300377075396No Hit
TCGCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC600.11845300377075396No Hit
TTGCTAGGGACAACCGTCATCTAAGGTTGTCTCTCATTTCCATCCCCCTCAA590.11647878704124139No Hit
CTAACAGTGGGCAATTGCAAATGATAAGCATTTTTGTGAGTGACCACCTTTG590.11647878704124139No Hit
GGGACTGGGAAGCAGTGTCAGGAGAGCACTTCAGGATTCATCCTTTCCTTGT590.11647878704124139No Hit
GCTCAGGGGATTCAAACATTTGTATGCGTATTTGTGAAATTGTTTGCATCAG590.11647878704124139No Hit
ACTGATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC590.11647878704124139No Hit
CTTAAAGGGGCTCTTCCGACAGTTGTGTTGTGCCAATGGTGGAGAAGAAAAC580.11450457031172882No Hit
AGCTGCGGGAGACACAAAGCCAGAGAAAACAAATTCATTGTTCGTGACTTCC580.11450457031172882No Hit
GTGATAGGGATGCGGATTCAGATCAACTGGTGTTGGAAGTTATGTAGAGATG580.11450457031172882No Hit
TCCCATGGGCATGTCTTCTGACATTCCTGGTGTCCCAAAGAATAGCAAAAAG580.11450457031172882No Hit
TGTTAGAGGGCTTGTAACAGTTAATGACGCACTGACGTTGCTCTTTTCCACC580.11450457031172882No Hit
ATTCTTGGGGTCTTTCTGGCTTGTTAGATCTTCTCATTTGGCGTCCTTTCTC570.11253035358221625No Hit
GTTTCCGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC570.11253035358221625No Hit
GATCACGGGACTTCAGACCTTGAATGATTGACTGAAAATGGAAGCTCCCATG570.11253035358221625No Hit
TAAAGGGCAGAAATATGACATGATGATTCATTATTGAATCATAATGCCTTGT570.11253035358221625No Hit
AGCGCGGGGCTCTTGGGCAGAATCCCACAGAAGCAGAGTTACAGGACATGAT570.11253035358221625No Hit
AATACTGGGATAGCGTGGATTCCCCGGAACAGCTGTCTGGAATAAGGATGAT570.11253035358221625No Hit
CATAGAGGGCATTATTAGAATCAGATTTGCATGTTGCATTCATTGTTCTCAC570.11253035358221625No Hit
GTAGCCGGGTGCTAGTTTTGGTTCGGTTTTGCAATGGTTCTGTTGTCAGGAA570.11253035358221625No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCC560.1105561368527037No Hit
GTTATGGGGAGTTCTATTTCAACATGTGGGTTCACAGATTTATTCTAACCTT560.1105561368527037No Hit
CGCATGGGGATTATGGGGATTATAGGGATTGCAATTCAAGGTGAGATTTGGG560.1105561368527037No Hit
TGCAGGGGTGGAAGATTCTCAGTCATATTCTAAAGAGTCTCTCTCTTTCCCA560.1105561368527037No Hit
CGGCACGGGTGCTGAGTCTGCAGGAGTTCTCCAACATGGACCTTCGGGACTT560.1105561368527037No Hit
TTGAAAGGGGTTGCATTAAACCTCACTGAAACCTGCTCCGTGCCCGGATGTT560.1105561368527037No Hit
AGTCAAGGAACTCTCTTCCCAAAAATATAAATAATATCTGAAAATATTTAAG560.1105561368527037No Hit
CCTAGAGGGGTGGCAATGGAAGGAGCAGAGCAGGAAGAACTTTTAAGTCTGA560.1105561368527037No Hit
GGCTACTGGGTGTGTACATATTATCTGCATACTTGTTATGAAAGACGTAAAG550.10858192012319111No Hit
AAGCTTGGGTAAGAGTTTTGAACATGCTATTACACATTGTGAATTTATAAAA550.10858192012319111No Hit
ACATAAGGGGAAGTGGAGCCCATCATCCTCTCCGTGGGCGAGGAAGATGAGG550.10858192012319111No Hit
AACCTGGGCCTTGTAGCTTTATTCCTTGCATCTTTGGGTCTACTGAGCAGTG550.10858192012319111No Hit
AAAACGGGGAACCGGGCCGGCTTCAGTGTCGCCGCCATCTTTGTTGATGTGT550.10858192012319111No Hit
TTTTAGGGGAGCATTACTCAGTTTTGGTGGTAGTTGCTTAATCTTCACCCCC550.10858192012319111No Hit
AACCAGGGGATGACGCTCAATTTTAATTCCTTCTGTTACTCATGACATTCAC540.10660770339367856No Hit
GACAAAGGGGTGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC540.10660770339367856No Hit
TCAGCAGGGATTGGGGAGCATTCTGATGCAGCAGAGCTTGGAAGGATGCTTC540.10660770339367856No Hit
TTGCAAGGGTTCCTACTGTGTAGGAGAATTTGCAGTCAGCCATAGGTATGTA540.10660770339367856No Hit
TGCCAGGGGCTGACATTAGTGCACATAATGCAGAGTTTAACCTTGATTCTTC540.10660770339367856No Hit
GCTTTGGGGTAAGAATAATGACAAATGTTTTTTTAAAAACTACATGTTGGAA530.104633486664166No Hit
GTTAAAGGGATCAAGTGATAGTAAAATGACTAATAACAGTAAAGGAGTTTTG530.104633486664166No Hit
CGCGTGGGGTCAGTTATAGAACAAGTTCATTTTGCATGAACTTCTCTTATTG530.104633486664166No Hit
CAATCAGGGAAGCATTTCCTGAGAAGCATCTTCACAGAAATCATTGAAGTGC530.104633486664166No Hit
GTTCAGGGGAAGAATGGAAAGAAGTAGATGCATTTGAATGATACTCAAAAGG530.104633486664166No Hit
AAGCGGGGGAACCTTTTTTCCTTTCATTTTAGATGGAAAACAGCCGAAAAAT530.104633486664166No Hit
CCATCTGGGGAAATAAATTCTGAGCTGCTGCTTTTGATATAGTCAGACTTAA530.104633486664166No Hit
ACTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA530.104633486664166No Hit
AAGCTGGAGGATTACTTGAGCTCAGGAGTTTGAGACCAGCCTGGGCAACAGA530.104633486664166No Hit
TTTTACGGGGGGATGGGAATCTTTATCTTTTGGCTCCTGTCTTCCATTGGTC530.104633486664166No Hit
CACAAAGGGATATGTTTACTGGTGACAGCGTGAAAGAGCAAGTAGAGAAGAT530.104633486664166No Hit
AGAAATGGGCTGTCTTTTCTTGGCCTAAATTATTTCAGAGCTAGCACAAAAA530.104633486664166No Hit
CAGAATGGGGATCTCATTGCGGGGTCTTTACATATTTTCACCATTATTAGAA530.104633486664166No Hit
CAGTAGGGGAGTCATTCCTTATGAGAAAAAAAACGGACCACCGTCTGTTGAA530.104633486664166No Hit
ACACATGGGTGAGTGATTTTAAAATGTTTTGGTGAATGTGAAAACTAAAGTT530.104633486664166No Hit
CATCTTGGGAGTGCAATCAAACTAGAACTCAGGATTAAGAATCTCACTCAGA530.104633486664166No Hit
TCGCACGGGCGCTCCCAGGCAGGCTGATTAGATACCTGAAATACGAGAACAC530.104633486664166No Hit
TAGCGGGGATTAATCCTATCTCCAAAGTTAGTGGCTGTGATGCAAGCAAAGT530.104633486664166No Hit
AACGATGGGGGGCTTCTTAGGCCTGAAACGTTGGAAGCACAGGACCAAAGCA530.104633486664166No Hit
CGTTCCGGGAGGTGGCAGAGCTGCCATGCTGCGAAGCACCCTACGCGGCAAG530.104633486664166No Hit
TTTGTGGGGTCTGGTTTAAAAAATAAAAAGGTTTCTCTTAACAGTATTTCCA530.104633486664166No Hit
GCGCGGGGATGAATTCTCATGCATAATTTGATTTTAATTAAATCATAGATAA530.104633486664166No Hit
GTCTATGGGGTTTCTTTATTCCTAATGCAGTTAGAAAGACCTTTCTCCTTGA520.10265926993465342No Hit
CTAGCGATGGGATAGTATTCTCTTCACTAAAGGTAATATGTTGTCATGTTTA520.10265926993465342No Hit
CAACTGGGGGGAGACAGGTGGGGTACTCGGGAAGCTGGAGCGGGCCGGCGGT520.10265926993465342No Hit
ATTACGGGGGGCCTGGAGCCGCCGGGAGTGGACGCCGCCGAGGCCCGGAGTC520.10265926993465342No Hit
GTTCCCGGGAAGACAGACCAAGTGTATGCTCACGGAGCTGAAATGCATTAGT520.10265926993465342No Hit
TTAGCGCCGGGCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACA520.10265926993465342No Hit
ATGAGTGGGCGGATGGTCAAGTTATCTGACCATCTCCCCTCCATAAACCTTT520.10265926993465342No Hit
GCTTCTGGGTCACGGAAAACATTGGTTGCAAAGCCCCTCAAAGCCCTCAAGT510.10068505320514087No Hit
AACAGTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC510.10068505320514087No Hit
CTAGATGGGATGTAGAAGTAAAGAGCAAATAGGAAATGCTGGTGGAATTTCA510.10068505320514087No Hit
AAGAACGGGGTCCTGAGATGGATTTAATTCTTGATGCTGGTGCTAGAAGTAG510.10068505320514087No Hit
GCTTGCGGGGACTTCCAGAATTATGGCGAAGTCCGGGCTGAGGCAGGACCCG510.10068505320514087No Hit
TATTTCTGGGTTGCTGCATCAAATGTAAACGACGAACAAGATGATGAAGCCG510.10068505320514087No Hit
GTGGAAGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC510.10068505320514087No Hit
AGGGCTGGGGCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGG510.10068505320514087No Hit
TTGGCCGGGAACCATAATAACAAGTCTTGTCTTACCCTAAAGTCAAATGCTG510.10068505320514087No Hit
GAAATGGGGCTGGGTAGTAGGAGTAAAAGGAGGAAACGATCTGAAGAGACCT510.10068505320514087No Hit
TGGCATGGCTTGTTAGGGCTTGTACCTTGGGCAAGTTACTAATCTTTTTTCT510.10068505320514087No Hit
CTGGCCAGGCTGTGCCAGTGTGTCCCGGACGCATCACTAAGGAAGAGAGAGT510.10068505320514087No Hit
GCAGCAGGGCGTCATCCAGCCCCACCAAGTGACTTTGGTTGACTTGTGGGCT510.10068505320514087No Hit
GTAAGGGGATACGGAAGAAAATAAAAGAGTCTTTGAGAAAGCATTGGGAAAC510.10068505320514087No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)