FastQCFastQC Report
Sat 31 Dec 2022
SRR5848175.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848175.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74007
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGCCGTCT6240.8431634845352467No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGC6150.8310024727390652No Hit
AAATTGGGAACAACCTTTTACATTGTGACTGTGTTTGCATTGTCTAATGACA1220.164849271014904No Hit
GAATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1170.15809315335035876No Hit
GCGACTGGGATTTATGTAAGCAGTAAAGAACGGGGTGAAGGACATAACAGTG1130.15268825921872256No Hit
AGCTTGGGGGAACTTAAATTAAATTGACTTATGAATGCTTTGCCTGTGGGCC1120.1513370356858135No Hit
GCGACTGGAAGGACAAGATGGAAAGAAAATACAGGGAAAAGTGTTCAGAGCT1100.14863458861999543No Hit
GCTTAGGGGCACACGCATAAATTCACAGATAAAATTCTTAAGCATTTCAGGA1080.1459321415541773No Hit
TCACATGGGGGTGGTATGTAACAAGAGTTACGTATCCAGGGGCTTGTGCCTT1040.1405272474225411No Hit
AGTCCAGGGGCCTTTTCATATCCTCAGAAACAGGAGCCACCAGAAATGGAAC1040.1405272474225411No Hit
ATGTGGGGCACTTGAGTGTCACGAGGCAGAGCCTGGAAACATTAGCTTTGGA1040.1405272474225411No Hit
AAAGCTGGGAGGAAAAAGAAGGTTTGAAAACAAAACCTGTGAGCAGATTGTT1030.13917602388963207No Hit
ATGCTGGGGAGGGTGGGGAACGCGGGAGCGGGGCCAGCTCCCAGGAAAGCTG1030.13917602388963207No Hit
ATTTCCGGGGGCGCTTGGCCTCCCTAGTGCGGGCTGGCAGTGCGGGCAGAGC1010.13647357682381397No Hit
CCTCATGGGGAGAGTCAGCTAAGTGGGGAAAGGAAAAATATGCTTTTCTCTT1010.13647357682381397No Hit
TTGACTGGGAGTGCTATTGATTTGTGTGCGTTGATTTTGTGTCCTGAAATTT1010.13647357682381397No Hit
GATTCCGGGATCTGTAAATTAGATGGATGTTGGATGGAAAATGACATTGCTA990.13377112975799585No Hit
AAAGTAGGGAGCGATTATGAAGTGGCTCCTCCTGAGTACCATCGGAAAGCTG990.13377112975799585No Hit
CTACTTGGGGAAGGGTGCTTTCACTAAGGTTATATTTTAAAGTAGAATAACA980.13241990622508681No Hit
CGTCCAGGGAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCT980.13241990622508681No Hit
ATATTGGGGAAGAAGGGAGATGTGGTCATTGTGCTGACCGGATGGCGCCCTG960.12971745915926872No Hit
GTTACTGGGAGGAGCACCTGAAGCAAATATTTATCCGTATTTATTGAAAATT960.12971745915926872No Hit
TTCGGTTGGGATTAGGAATAAGAAAGTAGACATAGAGAACTGAAATATTATA940.12701501209345062No Hit
TACACCGGGGATTATGAGGCTATGTTTGGAGATTCTCCGAATGTGGTAGAGA930.12566378856054158No Hit
CGCTCTGGGCGATCTAAGGCGGGGTAATTAATGGATGTGGAGAGCATTTTAT930.12566378856054158No Hit
TCAGAAGGGGTTTGAAATGACCCTTGAGTCCCATGCAAAAGTTTCCTGAAGC930.12566378856054158No Hit
CACGCTGGGACAATAAAATTAGCTTTGCTTTATCAACAGATTATTTTCGGGC930.12566378856054158No Hit
CGCGCGGGCGAGACAGCTACCAAGTGACTCACAGGTGGGTAGCATGGATAGT930.12566378856054158No Hit
AAATGTGGGATTCCAGCCTGTGAAATAAAGGAGACCCCATCTCTAAGAAAAA930.12566378856054158No Hit
AATCCTGGGAGGAAAAAAGGAAGATTGAACTAATAAAATTTTATTTCTTAAA930.12566378856054158No Hit
GGCTCAGGGATTGCAATAAAGTATAATGTTTATTAAATGCTTCATTTGTATG920.12431256502763252No Hit
ATAGCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA910.12296134149472346No Hit
GAGCAGGGGGAGGAGGCCTACAGCAACTACTGCAAGAGGCAGGCCCAGCAGT910.12296134149472346No Hit
CTGGCTGGGTATGGAATTTTTTCCTACTGGGTAGCATTGTTGGCACACCTTG910.12296134149472346No Hit
ACACCTGGGTAAAGGGTAATTCCCCAACCCTTCCTTATGAGCATTTTTAGAA910.12296134149472346No Hit
AGCCTGGGGGTGTATACATATGCATACACACACATTTAAATGGAGTATGTTT900.12161011796181442No Hit
CAGCACGGGATAGCTTGCCAGGTTTACAAACTCAGACCCTTTTAAAAGTTGT900.12161011796181442No Hit
AAAGCTGGGATGGTGGCTGTTTGCATTATATATCTTTTTGTATCCTTTAATT900.12161011796181442No Hit
TTCATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC900.12161011796181442No Hit
CAGTAAGGGCAGCAGATGCACTTGAACGTGGGGCTCAGGAAGATCAAAGAGA890.12025889442890537No Hit
CAGCAAGGGGCGGACGGGAACCAACCCCACAAAAGTGCAAATAATCCTCAGA890.12025889442890537No Hit
GAAACCGGGAAGCAGGTTGAGAGGCATATACAACATCAGCTGGGTGGATTTT890.12025889442890537No Hit
AGTCTTGGGATTCAACAAGTAGACGTCTATTGAAAAGAAATTTTCCTAAGTT890.12025889442890537No Hit
ATTCTAGGGACGACTAGCAAAACTTCTTCGGAAAAGAGCAAAGAAAAATCCA880.11890767089599633No Hit
TTGCCAGGGAAGACCACAAGGTCAATGGCATGTCTGGAATTGTACATTAAGA880.11890767089599633No Hit
AGCGTGGGGGGACTTGGGCCTGGTGGAAGGGCTCTCAGCCCAGGGATCAGGG870.11755644736308729No Hit
CGCGCTGGGATCTTATTAACATCATGATGAATAAATTAGAATAGAAATTAGA870.11755644736308729No Hit
CACTAGGGGATACTCATTAGGAGCAATAATTACAGATTCAGTCATTTTGAAA860.11620522383017821No Hit
ACGTCCGGGATTCATTATCTGCAAATTAAGACTCACCTTCACTTTCCAAAAT860.11620522383017821No Hit
CGAACCGGGAGATAAATAAAATTATTCAAAGAAACAAAATTTTCTTTCTTAC860.11620522383017821No Hit
TCAGCACGGGTGGGCAGGAAGGCTGGGAGGAGCATGCCACCTTTGCAGTGAT860.11620522383017821No Hit
ACATATGGGATCTGACACCACTTTGGACTCAAGAGACTCAGTAACGTATTAT860.11620522383017821No Hit
ATGCACGGGCAGAGAAACGGCAGGAAGACCCTTACTACTGTCCAAGGGATCG850.11485400029726918No Hit
CCGCCGGGGGTACTCAAGATGGCGGCTCCGGGCGGGCGTGGCCAGTGACTAG850.11485400029726918No Hit
AATTTAGGGAATACTGTGATCATATGGATCTAAATACATAAGCACATAAGCC850.11485400029726918No Hit
ACGTTGGGGGATTCCTTTGGTATGGTGGGGAGAAGAGGCATGAGAAAGCTAC850.11485400029726918No Hit
TTTGCCAGGGAAGCCATCCTGGGCTGCATGTGGCCCGCAGGCTGTGGGTTGG840.11350277676436013No Hit
GGCCATGGGAAGTGAATTTTCTTTTCTCCAAATAGTGTGAGTTAAATCATGT840.11350277676436013No Hit
GTTTACGGGATTGCTGGCTTTTGTGGTGAGACGGAGGAAGATCACGTCCAGA840.11350277676436013No Hit
AACAGAAGGGGGCGCGGGTGCCGACGAGTTCCTGCCTCTGCTGAGCCTCGTC830.11215155323145108No Hit
CAAAGCGGGATTCAAAAGTTGAACTTCTACATTGTTTGCAATAGCTAAGAAC830.11215155323145108No Hit
GCCATTGGGGTTCTGATGGCTTTTCTTGTGGGTTCTATCTCCTTTTCCCTTC830.11215155323145108No Hit
GACCATGGGAAACCATCAGTCTCCTGAGACTCACTCATTGTCTCAAGAACAT830.11215155323145108No Hit
ACAGCTGGGATTTGCATTACAAAGGAATTCTTAATAATTCCTGTAAGCCTAG830.11215155323145108No Hit
CAGTACGGGATCTGAAGATGGTTGTTTCCACTTTGGAAGAAACCTATGGACA830.11215155323145108No Hit
TAGAGAGGGAGCCTCGTTACTGCATTTAGTTGCTAATGTGGATTAAGTTTGC830.11215155323145108No Hit
CGTAGTGGGAACACATCTGATGATACAAGGAGATTCGCAAAATTCTTCTTTG830.11215155323145108No Hit
CTACCTGGGGTCTTCTCAATGAATAATGATTGTCAAGCCACTCTAAGTTTTT820.11080032969854203No Hit
AAGGCCGGTGCACTCCCATATTCACTGCAACATTACTCACAATAGCCAAGAT820.11080032969854203No Hit
CCTTCTGGGGTGCCCTAGCTGGGCAAACCCTTCTCTTCTCCCCCCAACACAA820.11080032969854203No Hit
GTCTGCGGGATAAACAGTTTTACTTGTGTCATATTTTAAAATTACTAACTGC810.10944910616563297No Hit
AGTGCTGGGGACTGAGCCGCTGAGGGTGCGGGCTGACCCTGTAAGTGGCTGC810.10944910616563297No Hit
ACACTTGGGTCGAATTCTCATATATTACTATTGGGAATCTAAAATGATGTAG800.10809788263272394No Hit
GGCGATGGGATTTTTGAGCATTTGTCTATATTTGGTGAGAGAGAATAGATAC800.10809788263272394No Hit
AACTGAGGGGAGGCAGTGGCACAGTTCAGCAAATCTATTGAAGTCATTGAGA800.10809788263272394No Hit
GCGTCTGGGAAGGACTGATTCTTATTTTCCTTCTTAGTCTTCCATTTACAAA790.10674665909981489No Hit
CGCACTGGATATAAATTTATTATTCCTCTTTCATTTTTTTGAAACATGCCTA790.10674665909981489No Hit
AATTAGGGGGTCGTCCAGGTGTGCGAAGGAAAGAAGAAGCAGACCAACTACT790.10674665909981489No Hit
CTGATAGGGAGGAGTATGGAGCCAAGATCTAAGACCAACACTTGAGCAGGGG790.10674665909981489No Hit
TTGCTGGGGTATGAATGCTAAGAAGGCATAACCTACATCCTTCTCTGATTTC790.10674665909981489No Hit
GTAAGTGGGATAAGCACTTATATTCAAATAGAAAAACTGTCTTTGGACCTCT790.10674665909981489No Hit
TACAGCGGGATTGATATTGATTTGAAAATATTTTGGAATTTTTTCTACTTGA790.10674665909981489No Hit
ACAAGAGGGACATGGACCAAAAGATGTGCCATATAAAAATACAAAGCCTCTT790.10674665909981489No Hit
TTCTGCCGGGAAGTTTGAAAAGTGTCATGGCCCTGCTGACACATTTATTTTG790.10674665909981489No Hit
ATCTTGGGATGCACTCTGAATCAGGGTTTTTCTATAAGAAATCTTTCAGAAT790.10674665909981489No Hit
TGTTGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGC780.10539543556690584No Hit
TAGCGAGGGATACTAAACAACTGAAGATAGATAGTTTAGAAAGATAAGGACC780.10539543556690584No Hit
ATTTCAGGGATTGAATGTGTCTTAAAGGGACTATCAACCAATAATTCTATAT780.10539543556690584No Hit
TCCCGGGGTCACAAGGATATCTGGCAAAGGAAACCAAGCTGCTTCTTGACAT780.10539543556690584No Hit
TAGGTAGGGAGATTGGCGGTACCGGGATGCAGCAGTGATGGCTTTTGGTTGT780.10539543556690584No Hit
CGATTAGGGGTTAATTGAATAATCTCTATTTAATTCCACCCTTTTGAGGTTT780.10539543556690584No Hit
GTTAGTGGGCACCTCTGTGCCCTGGGCAGGCTCCAAAGGAAAGCTCTGGCTG770.10404421203399677No Hit
AAAGAGGGGAGTGCAGTAACATGCTGTACAGGTTTGTCACATAGGAACTGTA770.10404421203399677No Hit
CCGTTGGGTGGCTTGGGACTGTCTAATGAGATTGGAGCTATTGAAAAGGCTG770.10404421203399677No Hit
AAAGAGGGGTGACTCCTTGTAGTTACACTTGTGTTTCAATCAGATCAGATTG770.10404421203399677No Hit
GAATTAGGGGACAAGCAGACAAAATCGTTACAGATTTTTCAAGAGTTTAGGT770.10404421203399677No Hit
AATGCTGGGTCTCTTCCGGTTCTAGGCGCTTCGGGAGCCGCGGCTTATGGTG770.10404421203399677No Hit
CACATAGGGCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCC760.10269298850108773No Hit
CCACAAGGGATGGGGAGAAGCAGTTGAAGCATACTAGGGAGGCATGAGGTTC760.10269298850108773No Hit
CATAGCGGGAGCTTTAAGATGTTACCCTTCCAGAGGTACAGAAGAAACCTAT760.10269298850108773No Hit
TTCATCGGGTATATATACACACTAAATATTTGGGACACAAAGTTAAGCAGGT760.10269298850108773No Hit
TAGAAAGGGGCAGTTGCTATATTAAAATTAGGGCATAGAGCACAGAAAAATC760.10269298850108773No Hit
GTTTAAGGGAGTGTTTGAAACAAGTCAGCAGAATTCCATGCCTGCCCTGATC760.10269298850108773No Hit
ACATGGGGTCATCTTCAGGAAGGAAAAGGCATCCTGGAGACAGGAGTCCATT760.10269298850108773No Hit
GAAGATGGGACAATGTTAAAATGTTAAATATTTGGATATAGCAACTTCTTTT760.10269298850108773No Hit
CTATTAGGGCAACAAAAAGTAATATGAATTGATGGAAACAGAGAGATAGGTA760.10269298850108773No Hit
ATCAAGGGGATACATGCGTATTTTAGGGTATAAGGCTAAATTCAAATCTGTG760.10269298850108773No Hit
ATCTTTGGGTGACGATGAAGAAGCCTTGGATGAAGAAACAAAGAGGAGAGAT760.10269298850108773No Hit
TTGCATGGGAGGAAGATATGCTAGGAAACAGATTATTACTTCCAACACCAAC750.10134176496817869No Hit
CAAAACGGGGACCTGATTTAACGTTTATTACGTCCTTATACACCTTTCTAAA750.10134176496817869No Hit
TTCAATGGGGTCAGTTGGTGTAGGTAACATCCACAAATGTTTTGAGTTATAG750.10134176496817869No Hit
CGTCTTGGGGTCTGCAGCCTGTGGACCCCAGCCCTAGGGGCCTGCCATTCTG750.10134176496817869No Hit
ACGGGGGAAACAGAGGCAGGGACCACACTGTATCTACACAGTTTTAAACTGA750.10134176496817869No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)