FastQCFastQC Report
Sat 31 Dec 2022
SRR5848166.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848166.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61330
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGCCGTCT5790.9440730474482308No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGC4480.7304744823088212No Hit
CGAACAGGGCAATAAATATTAAATTTAATTCAAATTGGATCCCAGTTATAAA1640.2674058372737649No Hit
TAAACAGGGGGGGAAATACACAAAAGGACTGAAAACGGGCTCAAACAGATGT1520.24783955649763573No Hit
ATCTTTGGGTCTTGCGGTGGGGATTACACACACACAGACACACACACATACA1150.18751019077123757No Hit
TAAGCTGGGTATAAGAAGAAAAAAACTCGTTATACTTTCCATTTCCAAAATA970.15816076960704387No Hit
AATTGCGGGAAGTCATGAATGGAGCCAGAATCACATAGGATTCCGATATTGG880.143486059024947No Hit
GATGACGGGACTCTCTGAAATCACATAAATGACAACCAGTTTACTATTTACA860.14022501222892547No Hit
AAAAAAGGGGATCTATATCTGGGTGTGTATACTGGCTTACTTGTTGAGTTGG860.14022501222892547No Hit
AGCTAAGGGATTGCAGTATTATCCATAGAAATTAACTAAGACGCAGCAAAAA850.1385944888309147No Hit
TTTACGGGGTCAGTGAGATTGTGTGCCTAGCACAGTGCCTGACTCCCAGTAG820.13370291863688244No Hit
CTCAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC780.1271808250448394No Hit
CATGAGGGACCTTCTCAGAAGGCACCTCTCTCTGAGCCCTCCGCACTCCTGT770.12555030164682865No Hit
GAGGTGGGTGGTTAATGAGTACAAAAATATAGTTAGATAGAATGAATAAATC770.12555030164682865No Hit
AATGCGGGAGGGATCGGGTTTACTCAGAAGTTCGATGTCTTACTCCAACAGA760.12391977824881786No Hit
AGCTCTGGGGTTCCAGCTACTGAGGAGGCTGAAGCAGGAGGATCCCTCGAGC760.12391977824881786No Hit
TATATGGGGAGGAGTTCGAGACCAGCCTGAGCAACATGGTGAAACCCCGTCT760.12391977824881786No Hit
TTCCATGGGAGTCCTAAAGTGTACTTCATGAAGCAGACCATTGGGAATTCCT750.12228925485080712No Hit
GCGATGGGGTAGTTTGCAAAAATTTTCTCCCATTCTGTAGGTTGTCTGTTTA750.12228925485080712No Hit
TATGCTGGGATTGCAGGCGTGAGCCACTGCGCCTGGCCAAGGCATCATCTGT740.12065873145279635No Hit
GCTTCGGGGAATACAGAAATATCTATTCTTGCCTATCCATGATGTACAGCCA740.12065873145279635No Hit
TTGTCCGGGTACCTCTTCAATCTCATACCTGGAACATGTATGTATTTTCAAG740.12065873145279635No Hit
CGTCAAGGGGTGGCTACCAAATGGAACAGTGCAGGCTAGCCTAATCAGATAA720.11739768465677482No Hit
TCCAGGGAGCCTTGTCTATCTTCAGACACAAAAGGTAAATTAAATATTTTTA720.11739768465677482No Hit
TAACAAGGGGCAGTTTAACTTTTAATCCTTACCAAACATATGTTATTCCTTT720.11739768465677482No Hit
TGTCGCGGGGACCAATGATGAGTATTCTGGGGTGTCTGAATCAATGATTTTG720.11739768465677482No Hit
GTCCAAGGGGCCCAGACACCTCATTGTACTTGAAAACTGGAACAGCTTGGGA710.11576716125876406No Hit
GCGTTTGGGGAAACTGAATCAGTACAATGGAACATGAATTTTCAATAAAGGA710.11576716125876406No Hit
GCTAATGGGGTGATATTACTTATGCAGGTTAAACCTAAACATCTGTTGCATT700.11413663786075331No Hit
ACCCATGGGGCAAGAAAGATGAAGAGGTAAAGGGCATTGAAGCAGAAATGTA700.11413663786075331No Hit
TTACGGGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTG690.11250611446274254No Hit
TGAACGGGATTTTGGAAAGAGTTGATTGACCCAAGACTGATTATTGAATCTC680.11087559106473178No Hit
TATAACGGGGATCTTACCGAGGGTGACAAATTTTTAAAATATTTTCTTTTAA680.11087559106473178No Hit
GGACCATGATGATGTGAACGACCAGAGTGTCTCAAGTGCCCAGACCTTCCAA670.10924506766672101No Hit
AATGCTGGAACCTATAAGAAAGTTAATTGGAAGGATCTATGATACACACAGA670.10924506766672101No Hit
ATCGGGGATCATCACTTCCAGCACTACAAAATTATGCCATGCATGTTATTTA670.10924506766672101No Hit
TTAGGCGGGGTTGCTCAGGCACGAAGTACACACAAGAACTTTTTCTTAAGAC660.10761454426871025No Hit
CATGTTGGGAGTGTTGATTCTAAAAATATTATTCATTAGTATATATCAATGC660.10761454426871025No Hit
ATAGCCGGGCAGGGAAAACAGAGCGGGCTGACATTATCGATTACATCTTTTC660.10761454426871025No Hit
CAAAGTAGGATAGCTAATGCATGCTGGGCTTAAAACCTAGGTGATGGGTTGA660.10761454426871025No Hit
TAGCCTGGGGACGCTCTTCCGATCTTGAACAGGGTTTCTCATTATAGTTAAT660.10761454426871025No Hit
AATTGTGGGTCCTGGTGGTAGATGAGTTGCTCATCTTATGGTCTATTGGTCT650.10598402087069948No Hit
GGATTTGGGGACTCTCCTAAAATTTGTGTAAAAGAAATGCCAGAGGTACATT650.10598402087069948No Hit
AGAGATGGGTAGATTGAAAGACAAAGGGAATTCACTTTTCCTTGGTGTCTTA650.10598402087069948No Hit
AGTTATTGGGGATCTTTGGCAGGGATTAAAAAATACAAAAAAATACAAAAAA650.10598402087069948No Hit
ATTTAAGGGGATGGTGATGTCAATTTCCAAAGCAGCTTCTCCAGCCAAAATC650.10598402087069948No Hit
TCGCGCGGGGGAAATGCTGTGGGCTTTCCATGAATCCGTTGTAATAAAACAT650.10598402087069948No Hit
ATACGGGGACAGCTGAGAGTGAAGCAAAAGTAAAAACCAAAGTTCGCTTTGA640.10435349747268874No Hit
TCGTGGGGAACTAGGTGCCGATGAGACTGCTGTAGCCAGTATTTTGGAACGC640.10435349747268874No Hit
CACGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA640.10435349747268874No Hit
GCGCATGGGCATAGAATTTCGTTTTTAGATTGCTGTACTGAAGTTAATATTT640.10435349747268874No Hit
TAGTTAGGGTGGTAGTGTGCGCCTGTAACCCCAGCTACTCAAGAGGCTGAGG640.10435349747268874No Hit
CGCTCTGGGCTAGGTAAGGATGGAGCATTTGATCATACTGGTTTCATTATAT640.10435349747268874No Hit
TGGCCTGGGTAGAGCTGAATGGTTGGTGTGGTGTGACAGAGGAACATCAGGT630.10272297407467797No Hit
ACTAGGGGGATTGTAAAGGCACTCATCATGTGATTTCGGGCATGCTGGGCAA630.10272297407467797No Hit
GCGCGACGGGCTAGAAAAAAGGAATTGGTTGTTTAGGGCACACTGTTATATG630.10272297407467797No Hit
TGTGTCGGGATAAGGCAACAGGTTGCTTAATCTTTCTTGCCTACACACATTC620.10109245067666721No Hit
ATAACTGGGAGGGGCCATCACCCTGAACAACCTTCTCCTGCGGAAGGACATG620.10109245067666721No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)