FastQCFastQC Report
Sat 31 Dec 2022
SRR5848164.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848164.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93130
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGC5120.5497691399119511No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGCCGTCT3210.3446794802963599No Hit
TTGCTCGGGAAAGAGCTCATTGTCAGAGTTTACGCAACTGTTTGAGTTGCAG2970.3189090518629872No Hit
AGAAAAGAGAAGAGAATATTGAACACGGCAAATAGTTGGAAAGTCATACGGA1360.146032427789112No Hit
TGTGGTGGGATCAAAATGAAAAAAAGTCTCAAGTTAATGACCTACATTTTTA1290.1385160528293783No Hit
AGCTGCGGGGGCCCCCGATGGCCGCACGTTTGTGTCAGGCGCCTGTGATGCC1280.13744228497798777No Hit
AATAAGGGATTGGTTTGGTATTTTTTAATTTTCTATTTGTCAGTGCTCTTCA1270.13636851712659723No Hit
AATTATGGGCTGCTCATGGGATTCCTGGTGAAGTTGTGAATGGAGCTAGGAG1250.13422098142381617No Hit
GCTTACGGGAATCACCAAGGGCTTACTATGCATCAAGGTATTCTGGGGCATA1250.13422098142381617No Hit
CGGCTAGGGGACACCAGAAGTGAGCAGGAGTATCTATTCATATAGCAGACAA1180.12670460646408246No Hit
TACATCGGGATTCTGTGGAGAAGAAGGGCGGGAAGAACTTCCTTTCAGGAGG1110.11918823150434876No Hit
CACAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1110.11918823150434876No Hit
GGCACTGGGTCTCAGAACCCAGGGAGAAAACCCAATGCCACCTTTCTAAGGG1100.11811446365295823No Hit
TATCCAGGGTGGCCTGGGCAAGAGACTCTATCTCAAAAACAATTTTTTTTAA1100.11811446365295823No Hit
GCCAATGGGTTTTTATGGGCTGTTTAAGAACCAGTACTAAGGATACATTCTT1100.11811446365295823No Hit
GGGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1090.11704069580156769No Hit
GCTCTAGGGGCAAATTAAAACATTATAGCTTATGCTAGTTTCTACATTTTTT1090.11704069580156769No Hit
TATTGCGGGCAAGGTGTGACTGTAGTGATAAGCAATTAAGAGAAGGCTGGTA1080.11596692795017717No Hit
TCTTCTGGGAGAAATCGAAACACCAAAGGGACACAAATAGGGAAGAAGAGAG1080.11596692795017717No Hit
GGCAATGGGACTAGCAAAATACCTCCAAAGTGTATTTGATGGGAGGATATTT1070.11489316009878664No Hit
ACCGGCGGGATGTTCAGAGATTGCAGTAAAGACAGGCATAAGAAATTATAAA1050.11274562439600559No Hit
TAGTATGGGAACTTGAAGTAAAAATAAATGATTGGCATTGCTTTCTTCCCCC1040.11167185654461505No Hit
TTATCCGGGGAGCAAACACAGCTTCCAAACCCAGGGCTTGCGCTTGCCTTTG1030.11059808869322452No Hit
CTGTACACGTGCGGGAGGAAGGTGTGGTGCTGGAAGCTGTGGCATGGCTAGC1030.11059808869322452No Hit
TTTCCAGGGATCCTCACTTTGACTCTGACAAAGAACAGAAATGCTAGGGGGT1030.11059808869322452No Hit
ATGCACGGGAAGGGCAATCACTCTTAAAAAATCATTACACTATGTTCTCTCC1030.11059808869322452No Hit
AATAGAGGGTCCTAAGTGGGATGTGAAATCATTCTGGCTTTAGAATTCCATA1020.109524320841834No Hit
AACATAGGGGTAGAAAGTTTATTCAAAGAGATAACAGATAGCTTCCCAAACA1010.10845055299044347No Hit
CTGATAGGGATTTGCAGACAGACACAGTGGCATGTGGAGACAACAGTGTGTC1000.10737678513905295No Hit
GCCCTTGGACCTGAGTTTAGTACTAGTTCACAAATGCCAGCAACACAGAATC1000.10737678513905295No Hit
GGCATGGGTACAGCCAAATACTTACAACCAAATGATCTTTGACAAAGTATAG1000.10737678513905295No Hit
TTGCCTTGGGGAATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATG990.1063030172876624No Hit
GTACTGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGA990.1063030172876624No Hit
GCGAGCGGGAAAATTAAGTATGTGAATGTTTCTTTGTATTCAGAATTAAACA990.1063030172876624No Hit
GCGAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA980.10522924943627188No Hit
GCTAAGGGGAGATTAATTCAATTATGTTTTATTCATGTAAGCTATTAATAAA970.10415548158488135No Hit
TGACTAGGATCTATTAAAAACATTGGAAGCTGGAAAGTTGTGGTCAAGTGGT960.10308171373349083No Hit
TAATAAGGGAAGATAGAGATTAATACAACTACTTAAAAAATATAGTCAATAG960.10308171373349083No Hit
TTGTCCGGGAAGAAACTTGGGTTTCACGTTTTTGCTTTTTAAAGAATTAACA950.10200794588210028No Hit
CGACAAGGGATCATAATTCTGTTACTGTGTACACATATTGGGAATGCTTTTG950.10200794588210028No Hit
GATAACGGGAGGAAATGGTGAATGCTGTTGTTTCAGCAACATTTGCTATTTA950.10200794588210028No Hit
ATCGTAGGGAGGTATTGGAACCCACGGCCTGGGTGTTAGTCCAGCCGCTTAG950.10200794588210028No Hit
ACGATAGGGCGGATCCAGGATTGGTATAGCCAAAATGGAACCTGTTTTCTAG940.10093417803070975No Hit
TTCACTGGGGCCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAG940.10093417803070975No Hit
CTCTGCGGGTCAGACAGAGACTTCGAAATAGCCATTTTGAGGAAACTCAAAG940.10093417803070975No Hit
GGCCGCGGGAACATTTGCCCAAGGGAATGGTGCCTACCTTGCAACTCAGCGT940.10093417803070975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)