FastQCFastQC Report
Sat 31 Dec 2022
SRR5848163.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848163.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90464
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGC4890.5405465157410683No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGCCGTCT3110.3437831623629289No Hit
TTGCTCGGGAAAGAGCTCATTGTCAGAGTTTACGCAACTGTTTGAGTTGCAG2480.2741422002122391No Hit
AATAAGGGATTGGTTTGGTATTTTTTAATTTTCTATTTGTCAGTGCTCTTCA1400.15475769366819953No Hit
GGCATGGGTACAGCCAAATACTTACAACCAAATGATCTTTGACAAAGTATAG1240.13707110010611956No Hit
CTGACAAGGGGAACTTATATATTGAACTAAATTTTTGCCAAATTTGGCTTAT1110.1227007428369296No Hit
TATCCAGGGTGGCCTGGGCAAGAGACTCTATCTCAAAAACAATTTTTTTTAA1110.1227007428369296No Hit
GCTTACGGGAATCACCAAGGGCTTACTATGCATCAAGGTATTCTGGGGCATA1090.12048991864166961No Hit
TTATCCGGGGAGCAAACACAGCTTCCAAACCCAGGGCTTGCGCTTGCCTTTG1080.11938450654403962No Hit
GATATCGGGATGACACTGCTTTGTGCCAGACATAGAGCTTTGCACTGAGGTC1080.11938450654403962No Hit
GTACTGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGA1080.11938450654403962No Hit
GTATATGGGAGTAACAAGCAGCAAGATTGAAATTGTAATTAAAAAGTTACCA1070.11827909444640963No Hit
TCAGTAGGGAAATTCTTCAGAGAATAACATGATACTTCAAACCAGACTATTT1060.11717368234877962No Hit
TTAGATGGGGACTGCGGCACAGAGCCGCGGCTTGCTTTCAGGACACGGGTCG1060.11717368234877962No Hit
TACATCGGGATTCTGTGGAGAAGAAGGGCGGGAAGAACTTCCTTTCAGGAGG1040.11496285815351963No Hit
TATTGCGGGCAAGGTGTGACTGTAGTGATAAGCAATTAAGAGAAGGCTGGTA1030.11385744605588964No Hit
TGTGGTGGGATCAAAATGAAAAAAAGTCTCAAGTTAATGACCTACATTTTTA1030.11385744605588964No Hit
CGGCTAGGGGACACCAGAAGTGAGCAGGAGTATCTATTCATATAGCAGACAA1020.11275203395825964No Hit
AATTATGGGCTGCTCATGGGATTCCTGGTGAAGTTGTGAATGGAGCTAGGAG990.10943579766536965No Hit
TTCGCAGGGGACTACAATAAAGCATTTTAAAATTAAGGTAACATTGGCTTTT990.10943579766536965No Hit
CTGATAGGGATTTGCAGACAGACACAGTGGCATGTGGAGACAACAGTGTGTC990.10943579766536965No Hit
GGGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA990.10943579766536965No Hit
CCACTTGGGAGGGAGGCCACTTGAGTGCAGGAGTTCAAGTTACAGTGAGCTA990.10943579766536965No Hit
TCATTGGGGCAGGGAGCCCCAGACTCTGGTAAATGCTCTGCTTCCTCCTGCC990.10943579766536965No Hit
AGCCGCGGGCGGGTGGATGATCTCTCCGTGGAGTGGGACGCTATGGGCGGGA990.10943579766536965No Hit
AGAAAAGAGAAGAGAATATTGAACACGGCAAATAGTTGGAAAGTCATACGGA980.10833038556773965No Hit
AGCTGCGGGGGCCCCCGATGGCCGCACGTTTGTGTCAGGCGCCTGTGATGCC970.10722497347010966No Hit
TGAACTGGGGTTCAAATTTATAGCAAGGGAGTTGAATTTGAAGCAGGATTGA970.10722497347010966No Hit
TGACTAGGATCTATTAAAAACATTGGAAGCTGGAAAGTTGTGGTCAAGTGGT960.10611956137247967No Hit
ATTGTTGGGGCGTAGCAGGCCTTCAGTAAGCACTTGTGGAGTTAATTTCTCC960.10611956137247967No Hit
ATGCACGGGAAGGGCAATCACTCTTAAAAAATCATTACACTATGTTCTCTCC960.10611956137247967No Hit
AGCCGCGGGCTTGTTTCTGACCCTAATGTTGAAGAACCGTCAAAGTTACTAC960.10611956137247967No Hit
TTCACTGGGGCCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAG960.10611956137247967No Hit
CACAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC950.10501414927484966No Hit
TGGCCTGGGTCTTCCGATCTGCGTGTGGGCCAGATTGGCAATGCCTGCTGGG940.10390873717721967No Hit
GGCAATGGGACTAGCAAAATACCTCCAAAGTGTATTTGATGGGAGGATATTT940.10390873717721967No Hit
CTGTACACGTGCGGGAGGAAGGTGTGGTGCTGGAAGCTGTGGCATGGCTAGC940.10390873717721967No Hit
CTCAGTGGGCTAAGATTATGAAGTAATTTCCCTATTAGGGACTAGAATGACT930.10280332507958967No Hit
GCTCTAGGGGCAAATTAAAACATTATAGCTTATGCTAGTTTCTACATTTTTT930.10280332507958967No Hit
AACGGGGATCAATTGACAGTTTGACACATTCTTAGAGCTCATTGTGAACTCT930.10280332507958967No Hit
TTGTCCGGGAAGAAACTTGGGTTTCACGTTTTTGCTTTTTAAAGAATTAACA920.10169791298195968No Hit
CTTGCTGGGGTGACACTAACTAGGTTTTCATCATTTATGGTATAAGAAGTGC910.10059250088432967No Hit
ATAAATGGGAGATGAAATACATTTCTAGTAGTACCACCAGCATATATTCTAC910.10059250088432967No Hit
ACACTAGGGGGGTGCTATTCCTGGTTCTGTGGTGGTTTACTTTGTCTACTAA910.10059250088432967No Hit
GATCTTGGGCCCTGTGTTGACAGGCAAGTCGAGGGGCTGCTGCTGGCTTCCT910.10059250088432967No Hit
AAAAATGGGTCTAGTGATTGTGAGTGCCTTGTGGGCAGAATCCTTGTCATTT910.10059250088432967No Hit
TTTCCAGGGATCCTCACTTTGACTCTGACAAAGAACAGAAATGCTAGGGGGT910.10059250088432967No Hit
CACTTAGGGATAAAGGGACACAGTATTAGTTCATAGTGCTGGGATCTATGAA910.10059250088432967No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)