FastQCFastQC Report
Sat 31 Dec 2022
SRR5848159.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848159.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61763
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGCCGTCT3310.535919563492706No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGC2220.35943849877758527No Hit
CCGTCTGGGAGCCTTCAGCAACCAAGGTTCTCCTGGGACCCAAAGTTTATGG1290.2088629114518401No Hit
ATCTTGGGGATTCGAAAACTGAAAAATAAGGCTAGTGTACTACAAAAGAGAA1060.1716237877046128No Hit
TCCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1010.16352832602043293No Hit
TGTAATGGGAGGCGGCCATGGCGGCAGGCGCCGGGGCCGGGTCCGCGCCGCG950.15381377199941712No Hit
CTAACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA920.14895649498890923No Hit
TCTCTGGGGAAGGGAAAGATAGTCTTTTCCAAAACAAATGCTGAAAGAATTT890.1440992179784013No Hit
AAAGCAGGGATGTGATATATTTCCCCTCTTGAAGAGGCAATTCATTAAACTC890.1440992179784013No Hit
GTAAACGGGACATTGGACCAGGAAGGCTCAGAAAGGAGCCCAGGCCACAGAC880.14248012564156534No Hit
AATGAAGGGTCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAG880.14248012564156534No Hit
AAACAGGGGGTTTAAAAATCTGTATGCATAAAGAATGCACCACTCAACTTTT870.14086103330472938No Hit
CGGCTGGGGGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGTCACCGCGCC860.1392419409678934No Hit
TTTGCAGGGGAAAAATATGAATAAAAATGAAAGGACAAACAAGAACCTATTA850.13762284863105742No Hit
GCTATGGGGAGCCACAGGCACATTCACAGGGCCCAAGGCAGGTGCAGCTGCA850.13762284863105742No Hit
GATCTACGGGAACCAATTTTATCCCCAATTCACAGGTGACAAAACTCAGGCT850.13762284863105742No Hit
ACTGGCGGGGGTAAGATCAGAGCTGCAGGAGTCCAGACACGTACTTTGTCGC840.13600375629422146No Hit
TAGCCTGGGAATTGTAGTAAAATCAAGACCCCAAATTGCATAGAAATATTAT830.1343846639573855No Hit
GGTCGTGGGATCCATCCAAATTAAAAATGTCTTCTCTGTGAAGGACCCCATT830.1343846639573855No Hit
AATGCCGGGGTGTGTGTGGTGCACAGGTAAAGCTGGAGCTGGGAACTTTTTA820.13276557162054953No Hit
GGATGCGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGG810.13114647928371356No Hit
GTTGGTGGGCATGAAGGATTTTCATTTCAGTGAAAGTCGCAGCAGAAGAGGG810.13114647928371356No Hit
GCAAATGGGATAATGCATCTACTGTTAATGGTTTTGGTTAGTTTGATATTCA800.12952738694687757No Hit
GGAGATGGGATTATTGGCAAATTAATACATCAACACTTAAATCATTGACTAT790.1279082946100416No Hit
TTTGTTGGGACAGGCCTCTGTCTGCACATTTATTTTTAACCTAACTCAAGTG790.1279082946100416No Hit
AGTACTGGGAGAGCCCGCGCGCCGCCCGCTCCGGGTGCTGAATCCAGGCGTG790.1279082946100416No Hit
GATGCAGGGTATCTTGTCACTGAAATTACTCAAGAATTGCAGGGTGATTCCA780.12628920227320564No Hit
GATACCGGGAAGTCCAGGACCCATTAATTTCCTGAGCCAATCAGAAATATAT780.12628920227320564No Hit
CCAGCCGGGCACTGAATAAATGATAAATATATATTATCTTCCTTGTGATTTT770.12467010993636968No Hit
AACCAAGGGATGTCGTCACAAGGATATCTGGCAAAGGAAACCAAGCTGCTTC770.12467010993636968No Hit
TCTTTAGGGACCCGAGATTTCATTCGAGATTCAGGTGTTGTCTCACTTATTG770.12467010993636968No Hit
GCACTGGGGTGGGAGAATGGAACCAAGTTGGAAAACACTCTGCAGGATAGTA760.1230510175995337No Hit
GATACTGGGATCTTCAGGGCGGACCCTGTAGCCCTATGTGTTGCTTGAATCT760.1230510175995337No Hit
CATGACGGGGAATCTACTTTGCTGACATGTCTTCCAAGAGTGCCAATTACTG760.1230510175995337No Hit
GTTTAGGGGGAGGCGTGGCCGGAGCTGCGGCGGCAAGCGGGCTGGGACTGCT750.12143192526269772No Hit
GAAATAGGGCGATCTTGTTGCGGGATACATTATCAATGCTGAAGCTGACAAC750.12143192526269772No Hit
ATCAATGGGACGACCCACTACGCCAGAAGGCAATGTTTCTGCAGAGCCTGGT750.12143192526269772No Hit
GTATTAGGGAGTCTGACTCCTATTCTCTTTACATTATTCCATGTGGCCTCTT750.12143192526269772No Hit
GAGTTAGGGGAAAAGTAGGTGTGAGGAGAGTAGCAAAACATGTTACTGAATA750.12143192526269772No Hit
GAACCAGGGCGAGCAGGGGGAGGGTGGGCTCCGCGGTCGCCGGTTTCTCTTC750.12143192526269772No Hit
TACAATGGGGAAAATTTAGAAAACTGTTTTTCTCCTCAAGGAAGCACCAAAA750.12143192526269772No Hit
AGATTCGGGGTCCTCTAGAAAGAAAGCAAAAAAAGACCAATTTAGGTAGGAT750.12143192526269772No Hit
TGAATATGGGGTAGCCATCTGTCGCCCTCTGCACTACCCAGTCATCGTGAAT750.12143192526269772No Hit
GCCACAGGGGACCTACCATGGGGTCTCCCCATGTAAGGTGACTGCAGAGAAA740.11981283292586177No Hit
TGATTGGGGACCCATCTCTGTGGAAGGCAGTTCAAAAAAAGTCAAGACTGAT740.11981283292586177No Hit
GGCTGCGGAGGTGGAGGTTCAAGACGGCCCCAATGAAGATGGGGAGATGTTC730.11819374058902579No Hit
TACTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA730.11819374058902579No Hit
ATTGTGGGGAATACCATGATCGATGATGTCCCATGCTTCGAGGAAAACCAAA730.11819374058902579No Hit
AGCTCTGGGTCTTCACTGCTTTTTGCATTTTGAGTTGTGTGCCTATAAAATT730.11819374058902579No Hit
CTCGCGGGGTGGGGATGGAGGTGGAAGCAGGTTGTCAAGTGCCACTCTTCAT730.11819374058902579No Hit
ACCCACGGTATAGGTGTGTGTGTGTAATGGACCTTATGCAAATCCAGAACCC730.11819374058902579No Hit
ATGTGTGGGACTAGCTTAGATGTAGAGCCTGATGCTCTTTGAAGCTGATCAT720.11657464825218981No Hit
TGGATTAGGGTGGCGGCAGCTGGGCTCACCGTGACTGTCACCCACAGCAATG720.11657464825218981No Hit
GTGTCAGGGAGAGTATTATTTCAACATTTTCATTTTCATAAAAATTTTTTTA720.11657464825218981No Hit
TGCAAAGGGTGGTAGTGGAAAATTCTCTCAGCATTTGTTTGTCTGAAAACGA720.11657464825218981No Hit
GCTCGCCGGGATCTGCCTCCCGGGTTCAAATGATTCTCCTGCCTCAGTCTCC720.11657464825218981No Hit
ATTGAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC710.11495555591535386No Hit
TGCGCAGGGAGTAAATAAAAAGAAACGAAGTTGAAAGACTTGCTGGAGGAGG710.11495555591535386No Hit
TTACGCGGGATGACCTGGATGGTGATGGCAAGATCACCCGAGTGGAGATGCT710.11495555591535386No Hit
TATCACGGGATCACTGTGCAATTAAATTAGAACTCAAGATTGAAAGGTCCAC710.11495555591535386No Hit
AACGATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA710.11495555591535386No Hit
ACAAATGGGGTCTTCCTGGGGGTCACCGTGGACCGATTTGGCCGCCGGGGCA710.11495555591535386No Hit
GATTGGGAGGGAAAGGAGAGAGAAGGAAAGGAGTGAGGTATTTCCCGTCTAA710.11495555591535386No Hit
GGAAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC710.11495555591535386No Hit
TTCGACGGGATCAGCTTCAGTGACCCAGGGTCTGAACTGCCTCCATCCTAGG700.11333646357851788No Hit
CGCTTTGGGGATCTGTGGTTGGGGAAAGCGATGGAGGAAAATTTACCAAGCA700.11333646357851788No Hit
GCTGTCGGGATGCATTGTGTTTTTTAAAAAGAGTATCTTATACATGTATCCT700.11333646357851788No Hit
GTCTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC700.11333646357851788No Hit
AGCCAGAGGAGATTGATCCCAACTATTAATACATTAAAATGGTATTGCATAT700.11333646357851788No Hit
AATCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA700.11333646357851788No Hit
AGACACGGGACATTCCATCCGTGGAAGAAATTGCAGCCAGCTTTAAGATTTT690.1117173712416819No Hit
TCCTCTGGGTCTATCTAATTGTTTTTGTAGCAGCTGAAACTAAAATAATTTG690.1117173712416819No Hit
CTAGCTGGGAATGAGTGGTGTGGGTTTGCTCCTAGGGTTTCTTGTATGGCAG690.1117173712416819No Hit
ACAGTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC690.1117173712416819No Hit
TTGGCAGGGAGAATGTACAGACTTTCAACCTCCCCAATACTGCTGTACCATC690.1117173712416819No Hit
TAATGAGGGACGGGGTCAAAATGACCCACACGCTGCAGTGACAAGAAGGGCA680.11009827890484596No Hit
TGCTAGGGGCGATCTCAGTATGGGGCTCCTTGGTATGCAATTAAATGACTTG680.11009827890484596No Hit
AATTTAGGGCGGCAATGTTGTATTTGCATTCTTTATCCCTAACTCTGAATCT680.11009827890484596No Hit
GTAAGCGGGATGGACCTCTCAGGAACCAGGAGCATGCAAGATTGCGGTGCTT680.11009827890484596No Hit
GGAGCAGGGCAAGAGCAGGATCCAAATATTTCCAATGGAGAAGAGGAAGAAG680.11009827890484596No Hit
GCAGTGGGGGTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAG680.11009827890484596No Hit
TAATTGGGGCTACCTGGCTGTGGACAAAAGGTTATCTGCTCTTGGCCATCTG680.11009827890484596No Hit
GTTGCTGGGAGTTTGAAAGCTTCTTTTAAAAAGAATTCCTCTAATATGACTT680.11009827890484596No Hit
ACTACAGGGACACAGTGGGATGTTAAAGGTTGAATGTCTTTGGTATCTATAA680.11009827890484596No Hit
TGATGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA680.11009827890484596No Hit
GATCGAGGGGAGAGGAAGAGGAAGCTGCTGAGGACTGGGGAGAAGCTGAGCC680.11009827890484596No Hit
GCAATAGGGACCAGACCAATGCCTCCACCTATGAATACATCGACATCCCATT680.11009827890484596No Hit
TCACATGGGATTATTGCAGCCCTAGCAGCACTCCACCTCCTATTCTTGCACG670.10847918656800998No Hit
GCGATCGGGACATGGACTTGTGGACTTGATATTCATGTTGTATGTGATTAGT670.10847918656800998No Hit
ATATGCGGGAACTTTGCAGAGAAAGAAGAAACAAGCTTGACTGCATTTTGTC670.10847918656800998No Hit
ACAGAAGGGAATTCAAACCAAGTGAAGCGAGTTCCTGGTCAAGGCTCATCTG660.106860094231174No Hit
ATCGAAGGGGATTTAGGACAGAGGTAAGCAAATGGGCCAAATCTGGCCCACC660.106860094231174No Hit
TCGTCAGGGAATTTAGAAGACAGTATTTCTGAAAAAGTATGAAACGACCCCA660.106860094231174No Hit
TCCAAGTAGCTGGGTCTACAGGTGCCCACCACCATGCCCGGCTACTTTTTTT660.106860094231174No Hit
GATGCGGGGACTGATCACAGGGTCCCTAGAATGAAATAGATGAGAAGCATAC660.106860094231174No Hit
GTCTAAGGGATATATGTTAAAGATCAGCAGAAAAATGGACAATGAAAAAGTG660.106860094231174No Hit
GGCTCAGGGAAGGGCAGCAGAGTTGGGTGCTAAGATCCTGAGGAGCTCGAGG660.106860094231174No Hit
TGCTCCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC650.10524100189433805No Hit
TACCAAGGGTTGCACAGGCCGGTCTCAAACTAGGATCCTCTTTTTATCCCTA650.10524100189433805No Hit
AAGCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC650.10524100189433805No Hit
TTTGCTGGGAGGGGGTAGTAAACTGAAACATAAAGTAAACATGCATACAAAT650.10524100189433805No Hit
TCCAGCGGGAGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTG650.10524100189433805No Hit
ATTGCTGGGTCTGTGACACATTGATAACATTTTTAGGTCGGCTGGTAGCAGG650.10524100189433805No Hit
TGCTACGGGAACAACAGCTGGTGCCCTGCTTTCAGATACTGGATTGTACTTT650.10524100189433805No Hit
GCGTATGGGCTGGGACAGCTGAGCCAGGGGTCCTACCTAAGGGTCAGGCCTG650.10524100189433805No Hit
AATGTGGGATCTCTTGAATACTTGAAATGTTTAATTAGATGCCACCAATAGG650.10524100189433805No Hit
ATAATGGGAGTCAGGAAGGACGTTGGTGTTGAGGTTAGCATACGTATCAAGG640.10362190955750207No Hit
GTTTCCGGGAAGCAACATTAAATAATTAGTTCTTGGACTGGTGTTTTTTCTT640.10362190955750207No Hit
AGGCCCTGGTGTCTGTTTTCTCTTCTTTGTGTTTCCTTTTGTTGTTGTTGTT640.10362190955750207No Hit
TAAACAGGGTCAGAGAAAACAAGAAAGACTATTTGGGTTTATCACAAATCTT640.10362190955750207No Hit
TATGTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA640.10362190955750207No Hit
TACTCAGGGTCGAGGAAGGAAGTAAGTACTTCTTAATTATTTTAAAAGAATA640.10362190955750207No Hit
AGCAAAGGAAATAATTAACAAAGTGAAGAGACAACCTACAGAACAGGAGAAA640.10362190955750207No Hit
CTGCAAGGGCTGGGTATTCCATGCAGCTAAAGGTCGAGGGCCAAGGTGGCTG640.10362190955750207No Hit
TACAGGGGCAACACTCCTGTGTAATCAGGTTCTCTGTCTTCTGGAATGATTT640.10362190955750207No Hit
AAAATGGGGATTCAGTAGGAGAGGATAAGGAAACATGTATTGCCCTCAGGCC630.10200281722066609No Hit
AAGTAGGGTAAGATATAGAATTTCACCAAAGACCTGGAATCTGTAAGAAAGA630.10200281722066609No Hit
GTTCCCGGGACAGGAACGCTTGGTAACTGTGGATGAAATTGGAGAAGTGGAA630.10200281722066609No Hit
CGCTTAGGGGTTACCTTATCAAATGAAATATTACAGCACCTAGAAAATAATT630.10200281722066609No Hit
GCACTAGGGAAGGCATTGAATCTCTAAAATGCTGTTCTCAATCATTGTCAGA630.10200281722066609No Hit
GCTCCCTTCCTGACCTCCCCTGACCCCGAAGATCACTACCTCTGTCATTCAG630.10200281722066609No Hit
ACAATTGGGCAGACTACTTAGATTATTCTAATCTAATCTATTAAATGCTAGA630.10200281722066609No Hit
TGGTGTGGGGTGATGGTAGCCGGGAAGTTGATTCACTCTAAGAAGAAAGGCG630.10200281722066609No Hit
TAGTCTGGGGAGCTCCAGTCCTCTCTCGCAGAGGAGCTGGTATCCGAGTCTT630.10200281722066609No Hit
TTCGATGGGTCTGGAGCTGGAGGCACCCCTCAAGATCTGCGGTGACATACAC620.10038372488383014No Hit
AAAAGTGGGTGGGAAAGGAAGATGCCTGTCCTCTCCGTGGAGGACCCTGGGC620.10038372488383014No Hit
GACTTGGGGTATGTTCCAGCAAGCAGTACAAAATTGGGCAGGTGAGTCATAT620.10038372488383014No Hit
GAATACGGGCTTCTATTTAGGGAAGAAAAGGGGGAAGTGTGTTTTTGTTGCT620.10038372488383014No Hit
CTACATGGGTTCAGTTCTTCATCATATCTCCCTGAACTATTGCAGTCAACTC620.10038372488383014No Hit
TAGATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA620.10038372488383014No Hit
CCCTGTGGGCTGTTTGCTTCCATTGTAGCTCTTTGACATGCTTTAAAATGAA620.10038372488383014No Hit
AGATCAGGGATGTACTATTTAACTTAAATATATTTTGTTTAATAGGAAATAT620.10038372488383014No Hit
CAGCCATGTTCCCGGGACCTCGGGAGCGTCCCCCACCGCCCGGTGCCATTCG620.10038372488383014No Hit
CTGATAGGGAAGCTACATATCTCAGGGACCGAAATCAAGCCCAAAACTGTAC620.10038372488383014No Hit
CTACGCGGGAGACCCTCTGTACTTCCCATGGTGATTTTGAGGAGCACATGCA620.10038372488383014No Hit
TTTACGGGCTGTACGCCAACACGGTGCTGTCGGGCGGCACCACCATGTATCC620.10038372488383014No Hit
CCTGGAGGGACCACTGTGTTTTCCTTCTATTAAACCAGAAGAAGTAAACAGC620.10038372488383014No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)