FastQCFastQC Report
Sat 31 Dec 2022
SRR5848158.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848158.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63489
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGCCGTCT3990.6284553229693333No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGC2500.39376899935421883No Hit
TCTCTGGGGAAGGGAAAGATAGTCTTTTCCAAAACAAATGCTGAAAGAATTT1130.17798358770810693No Hit
TGTAATGGGAGGCGGCCATGGCGGCAGGCGCCGGGGCCGGGTCCGCGCCGCG1110.17483343571327317No Hit
ATCTTGGGGATTCGAAAACTGAAAAATAAGGCTAGTGTACTACAAAAGAGAA1060.16695805572618877No Hit
CCGTCTGGGAGCCTTCAGCAACCAAGGTTCTCCTGGGACCCAAAGTTTATGG1010.1590826757391044No Hit
GTAAACGGGACATTGGACCAGGAAGGCTCAGAAAGGAGCCCAGGCCACAGAC980.15435744774685378No Hit
TTTGCAGGGGAAAAATATGAATAAAAATGAAAGGACAAACAAGAACCTATTA950.14963221975460317No Hit
ACAAATGGGGTCTTCCTGGGGGTCACCGTGGACCGATTTGGCCGCCGGGGCA950.14963221975460317No Hit
AGCAAAGGAAATAATTAACAAAGTGAAGAGACAACCTACAGAACAGGAGAAA950.14963221975460317No Hit
AAACAGGGGGTTTAAAAATCTGTATGCATAAAGAATGCACCACTCAACTTTT940.1480571437571863No Hit
AATGAAGGGTCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAG930.1464820677597694No Hit
GAAATAGGGCGATCTTGTTGCGGGATACATTATCAATGCTGAAGCTGACAAC920.14490699176235255No Hit
TGAAAAGGGAGCTTCCAGGTTCCTTATCTATGTTTGCAGTCTGATCTTCCAG910.14333191576493567No Hit
AAAGCAGGGATGTGATATATTTCCCCTCTTGAAGAGGCAATTCATTAAACTC910.14333191576493567No Hit
CGCTTTGGGGATCTGTGGTTGGGGAAAGCGATGGAGGAAAATTTACCAAGCA900.1417568397675188No Hit
AAGTCTGGGATTAATTGCCTATATGAAATTTACCAAATTAGACCAACAGTTA900.1417568397675188No Hit
GTTGGTGGGCATGAAGGATTTTCATTTCAGTGAAAGTCGCAGCAGAAGAGGG890.1401817637701019No Hit
CATGACGGGGAATCTACTTTGCTGACATGTCTTCCAAGAGTGCCAATTACTG890.1401817637701019No Hit
ATTGTGGGGAATACCATGATCGATGATGTCCCATGCTTCGAGGAAAACCAAA880.13860668777268503No Hit
AATGCGGGAATAAAAAAGATGGACTGAGCACAGAAGCTTTCATTCCACAAAC880.13860668777268503No Hit
TTTGTTGGGACAGGCCTCTGTCTGCACATTTATTTTTAACCTAACTCAAGTG870.13703161177526815No Hit
AATTTAGGGCGGCAATGTTGTATTTGCATTCTTTATCCCTAACTCTGAATCT870.13703161177526815No Hit
AATGCCGGGGTGTGTGTGGTGCACAGGTAAAGCTGGAGCTGGGAACTTTTTA870.13703161177526815No Hit
CTAACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA870.13703161177526815No Hit
GCAATAGGGACCAGACCAATGCCTCCACCTATGAATACATCGACATCCCATT870.13703161177526815No Hit
CCAGCCGGGCACTGAATAAATGATAAATATATATTATCTTCCTTGTGATTTT860.13545653577785127No Hit
TGCGCAGGGAGTAAATAAAAAGAAACGAAGTTGAAAGACTTGCTGGAGGAGG850.1338814597804344No Hit
GGTCGTGGGATCCATCCAAATTAAAAATGTCTTCTCTGTGAAGGACCCCATT850.1338814597804344No Hit
TGAAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC850.1338814597804344No Hit
TTTGCTGGGAGGGGGTAGTAAACTGAAACATAAAGTAAACATGCATACAAAT840.1323063837830175No Hit
ACTGGCGGGGGTAAGATCAGAGCTGCAGGAGTCCAGACACGTACTTTGTCGC830.13073130778560066No Hit
ACTAGGGGGGAACTACCCACAGCCTTCCTCCCCTCTTTGGGATGCGTCTGTA820.12915623178818378No Hit
TCCAGCGGGAGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTG820.12915623178818378No Hit
TTAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATA810.1275811557907669No Hit
TTACGCGGGATGACCTGGATGGTGATGGCAAGATCACCCGAGTGGAGATGCT810.1275811557907669No Hit
TCCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA800.12600607979335002No Hit
CTGATAGGGAAGCTACATATCTCAGGGACCGAAATCAAGCCCAAAACTGTAC800.12600607979335002No Hit
ATGTGTGGGACTAGCTTAGATGTAGAGCCTGATGCTCTTTGAAGCTGATCAT790.12443100379593316No Hit
GAGTTAGGGGAAAAGTAGGTGTGAGGAGAGTAGCAAAACATGTTACTGAATA790.12443100379593316No Hit
AGTACTGGGAGAGCCCGCGCGCCGCCCGCTCCGGGTGCTGAATCCAGGCGTG790.12443100379593316No Hit
GTTTAGGGGGAGGCGTGGCCGGAGCTGCGGCGGCAAGCGGGCTGGGACTGCT780.12285592779851628No Hit
TACTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA780.12285592779851628No Hit
GGAATGGGAAGGAAAAGCACCCCCCATGGCAGAGACACTGCACATGATTGTG780.12285592779851628No Hit
CGGCTGGGGGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGTCACCGCGCC780.12285592779851628No Hit
AGCCAGAGGAGATTGATCCCAACTATTAATACATTAAAATGGTATTGCATAT780.12285592779851628No Hit
AATGTGGGATCTCTTGAATACTTGAAATGTTTAATTAGATGCCACCAATAGG770.1212808518010994No Hit
ATGTTAGGGGAAAAAGCCTAAAGCAAAACCAAAAGGCAATTCTGGAGCTGTG760.11970577580368252No Hit
GTCTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC760.11970577580368252No Hit
GTTTCCGGGAAGCAACATTAAATAATTAGTTCTTGGACTGGTGTTTTTTCTT760.11970577580368252No Hit
GCATGAGGGCTGTACACCCCAGGACAGTTTATCCTGTCTGAGGAGTAAGTGT760.11970577580368252No Hit
GATGCGGGGACTGATCACAGGGTCCCTAGAATGAAATAGATGAGAAGCATAC760.11970577580368252No Hit
GATTGGGAGGGAAAGGAGAGAGAAGGAAAGGAGTGAGGTATTTCCCGTCTAA760.11970577580368252No Hit
GTGGAGGGTGTCCCTCCCACCTACTCTGCCATTGCTTGAAGTGCCGTTTCCA760.11970577580368252No Hit
TGTTTAGGGTCTTGAGATTTGATTCTAAATATGGGAAGATGGTGGTTAGATT750.11813069980626564No Hit
ACATATGGGTATGATTACATCCAATATTTTGAAACACATTTGTTGTCATCTA750.11813069980626564No Hit
TGCCCTGGGGAATGACAGAAAGAATGAATGGACACATGAACGACTGAATTAG750.11813069980626564No Hit
ATTTGAGGGGTGTCTGGGCATATTCGTCTGCTGATGGGGTTTGGGGAAGCCT750.11813069980626564No Hit
GTAAAGGGGGGACAAATTGAAGGTGTACGGCAAATGCACAGCAACGCACCAA750.11813069980626564No Hit
TACAATGGGGAAAATTTAGAAAACTGTTTTTCTCCTCAAGGAAGCACCAAAA750.11813069980626564No Hit
CTGATAGGGACTGCTTGTCTCACTAGAAACATTTATTTCTGACCTACCAAGT750.11813069980626564No Hit
CTGCAAGGGCTGGGTATTCCATGCAGCTAAAGGTCGAGGGCCAAGGTGGCTG750.11813069980626564No Hit
TCACATGGGATTATTGCAGCCCTAGCAGCACTCCACCTCCTATTCTTGCACG740.11655562380884878No Hit
TACTTTGGGCACCCATCTCATCTTTACAAATTATATGACTGTATTAAATTAT740.11655562380884878No Hit
CTACATGGGTTCAGTTCTTCATCATATCTCCCTGAACTATTGCAGTCAACTC740.11655562380884878No Hit
CTTTGCGGGGTATTTACTCAAAACACAATTTGCACTGTACACTAGTGAATTG740.11655562380884878No Hit
GTTTAGGGGCGGGCTGAGGGAGGTGCAGGGCCTTCTCTGACCCCTGCCTGAG740.11655562380884878No Hit
TAGCCTGGGAATTGTAGTAAAATCAAGACCCCAAATTGCATAGAAATATTAT730.1149805478114319No Hit
TAAGTAGGGGGAGAAGCCGCAGCGCCGCCTCTTCTCTCGCGCCCTCGCCTCT730.1149805478114319No Hit
AAAAGTGGGTGGGAAAGGAAGATGCCTGTCCTCTCCGTGGAGGACCCTGGGC730.1149805478114319No Hit
AGCTCTGGGTCTTCACTGCTTTTTGCATTTTGAGTTGTGTGCCTATAAAATT730.1149805478114319No Hit
CGTATCGGGGTCAGACTTTTCTTTTTAAAAATTTAACATGATTGCTTTTCTC730.1149805478114319No Hit
AACGATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA730.1149805478114319No Hit
GCACTAGGGAAGGCATTGAATCTCTAAAATGCTGTTCTCAATCATTGTCAGA730.1149805478114319No Hit
AAAGCTGGGGTCCTGACTTACCTGGCACCCTGGTCAGAGCTCTTTCTTGGTT730.1149805478114319No Hit
GCTCCCTTCCTGACCTCCCCTGACCCCGAAGATCACTACCTCTGTCATTCAG730.1149805478114319No Hit
TAATGAGGGACGGGGTCAAAATGACCCACACGCTGCAGTGACAAGAAGGGCA720.11340547181401502No Hit
TGCTAGGGGCGATCTCAGTATGGGGCTCCTTGGTATGCAATTAAATGACTTG720.11340547181401502No Hit
GCACTGGGGTGGGAGAATGGAACCAAGTTGGAAAACACTCTGCAGGATAGTA720.11340547181401502No Hit
GATACTGGGATCTTCAGGGCGGACCCTGTAGCCCTATGTGTTGCTTGAATCT720.11340547181401502No Hit
AATAGTGGGAGAGATGGGACTAGAGAAGTCACCTCAAGATTGGTGACAGATC720.11340547181401502No Hit
CGTTAAGGGACGTGTACCCTCTGTATCCAAAATAAAAGCTGAAAAAAAAGGA720.11340547181401502No Hit
GCAGTGGGGGTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAG720.11340547181401502No Hit
CAAAGAGGGGTCAAAGCGATTGATATTTATCTGATGGGTTGCTTTGTGTTTG720.11340547181401502No Hit
ACAATTGGGCAGACTACTTAGATTATTCTAATCTAATCTATTAAATGCTAGA720.11340547181401502No Hit
GCGTATGGGCTGGGACAGCTGAGCCAGGGGTCCTACCTAAGGGTCAGGCCTG720.11340547181401502No Hit
GATACCGGGAAGTCCAGGACCCATTAATTTCCTGAGCCAATCAGAAATATAT720.11340547181401502No Hit
GGCTGCGGAGGTGGAGGTTCAAGACGGCCCCAATGAAGATGGGGAGATGTTC710.11183039581659814No Hit
AAAATGGGGATTCAGTAGGAGAGGATAAGGAAACATGTATTGCCCTCAGGCC710.11183039581659814No Hit
TACCAAGGGTTGCACAGGCCGGTCTCAAACTAGGATCCTCTTTTTATCCCTA710.11183039581659814No Hit
AGTAATGGGGTGTCTACAGCTAAATTTTGTTTACAATTAGGCTATTTTTTAA710.11183039581659814No Hit
TCCAAGTAGCTGGGTCTACAGGTGCCCACCACCATGCCCGGCTACTTTTTTT710.11183039581659814No Hit
AGACGTGGGGAAATGGCAGCAGTAATGAGGAAAGCCATTGAAATTGACGAGC710.11183039581659814No Hit
GATCTACGGGAACCAATTTTATCCCCAATTCACAGGTGACAAAACTCAGGCT710.11183039581659814No Hit
AATTAAGGGGTGACATTTGAAAATGTGTCTAAACAATATTTGTAAGTAAAAG700.11025531981918128No Hit
CAAAATGGGGCAGCTGCAGCTCATTACCAGTCTGAAGGAGCAAGCAATAGGC700.11025531981918128No Hit
GTCCAAGGGAAATTCCAGGAAGTTGAAAGAATACAGACCACATCCTCAGATC700.11025531981918128No Hit
TTCGATGGGTCTGGAGCTGGAGGCACCCCTCAAGATCTGCGGTGACATACAC700.11025531981918128No Hit
ATAATGGGAGTCAGGAAGGACGTTGGTGTTGAGGTTAGCATACGTATCAAGG700.11025531981918128No Hit
GTAAGCGGGATGGACCTCTCAGGAACCAGGAGCATGCAAGATTGCGGTGCTT700.11025531981918128No Hit
TGATTGGGGACCCATCTCTGTGGAAGGCAGTTCAAAAAAAGTCAAGACTGAT700.11025531981918128No Hit
TATCACGGGATCACTGTGCAATTAAATTAGAACTCAAGATTGAAAGGTCCAC700.11025531981918128No Hit
TACTCAGGGTCGAGGAAGGAAGTAAGTACTTCTTAATTATTTTAAAAGAATA700.11025531981918128No Hit
AATAATGGGATTTTTTTAAAATGGTACACCAGAAAAGGACTGGCAGTCTACT700.11025531981918128No Hit
TACAGGGGCAACACTCCTGTGTAATCAGGTTCTCTGTCTTCTGGAATGATTT700.11025531981918128No Hit
TCTTTAGGGACCCGAGATTTCATTCGAGATTCAGGTGTTGTCTCACTTATTG700.11025531981918128No Hit
GCATTAGGGGGGGCCAGCAGCACCCCGGGGTCTGCCCAGGGGAGTCAAGGCC700.11025531981918128No Hit
GGCTCAGGGAAGGGCAGCAGAGTTGGGTGCTAAGATCCTGAGGAGCTCGAGG700.11025531981918128No Hit
GCCACACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG690.1086802438217644No Hit
CTCGAGCGGGATCCATTTCTTTGGTGATGCCAAGCAAATGAAACCCTGCCAC690.1086802438217644No Hit
GGTAGCGGGGTTATTCTAAGGAATCTTCATGCACTCTTTATCTTCCCCCATC690.1086802438217644No Hit
GTTGCTGGGAGTTTGAAAGCTTCTTTTAAAAAGAATTCCTCTAATATGACTT690.1086802438217644No Hit
TAACGGGGACCTAGCACTACACTTCTATTTATTTCCATTATTTTTCAGCTCA690.1086802438217644No Hit
AATTGCGGGGCATAGACACATGCTGATACCTGTGAGTTGCTTTCATCAATTA680.10710516782434752No Hit
GAACCAGGGCGAGCAGGGGGAGGGTGGGCTCCGCGGTCGCCGGTTTCTCTTC680.10710516782434752No Hit
CGAATAGGGCTTCTGACCTGCAGGACAGTTTTCCTGGAGCCATTTGGTATGA680.10710516782434752No Hit
AACCAAGGGATGTCGTCACAAGGATATCTGGCAAAGGAAACCAAGCTGCTTC680.10710516782434752No Hit
GATCATGGGAAGCCCACATATCAGGACCGAGTGGCCCCGCCAGGATTAACAC680.10710516782434752No Hit
GTCATGGGAGTGCTAACTGTTGAATTTAACAATGGATCCCATGACGGAAAAG670.10553009182693064No Hit
GCTGTCGGGATGCATTGTGTTTTTTAAAAAGAGTATCTTATACATGTATCCT670.10553009182693064No Hit
CTAGCTGGGAATGAGTGGTGTGGGTTTGCTCCTAGGGTTTCTTGTATGGCAG670.10553009182693064No Hit
GCGATCGGGACATGGACTTGTGGACTTGATATTCATGTTGTATGTGATTAGT670.10553009182693064No Hit
GCTATGGGGAGCCACAGGCACATTCACAGGGCCCAAGGCAGGTGCAGCTGCA670.10553009182693064No Hit
AGCCAGGGGAGTGCGCAACCGTTCGCTAACTGAAATGATGGCGACTGGAACG670.10553009182693064No Hit
GTGTCAGGGAGAGTATTATTTCAACATTTTCATTTTCATAAAAATTTTTTTA670.10553009182693064No Hit
TCTCGCGGGTTCTTGGGTGTACAGTAATTATATCTGTGTGATTTGAATGTAT670.10553009182693064No Hit
TGCAAAGGGTGGTAGTGGAAAATTCTCTCAGCATTTGTTTGTCTGAAAACGA670.10553009182693064No Hit
TTGGCAGGGAGAATGTACAGACTTTCAACCTCCCCAATACTGCTGTACCATC670.10553009182693064No Hit
TAAGCAGGGTACAACAAAGTCAAACTGCTGTTAGACTCAGGTGACAGTCAAA660.10395501582951379No Hit
ACGCAAGGGCGCTCTTCCGATCTGTCAGACGGGCAAGAGGCTGCCAGGAGCG660.10395501582951379No Hit
TCCTCTGGGTCTATCTAATTGTTTTTGTAGCAGCTGAAACTAAAATAATTTG660.10395501582951379No Hit
CGAGGTGGGGAGACCAAGGATAAAAGGATCGTTTTGTAAGACATGCCATGGC660.10395501582951379No Hit
ACATCCGGGACACAATTGAGGACTTCTGGCGAATGATCTGGGAGTGGAAATC660.10395501582951379No Hit
CAGCCATGTTCCCGGGACCTCGGGAGCGTCCCCCACCGCCCGGTGCCATTCG660.10395501582951379No Hit
ATATGCGGGAACTTTGCAGAGAAAGAAGAAACAAGCTTGACTGCATTTTGTC660.10395501582951379No Hit
ACAGTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC660.10395501582951379No Hit
GCGCAGGGACAGTGAAAGAGTAAATTAGAAAGTGAATGGATGACTGTTCATG660.10395501582951379No Hit
GGAGATGGGATTATTGGCAAATTAATACATCAACACTTAAATCATTGACTAT650.10237993983209691No Hit
AATAATGGGATACATTGTAAATTAGTAACTATGGGTATTTTGTTTTAAATCT650.10237993983209691No Hit
AGCTGAGGGCTGCACGGACACATTCCAAACCAACAGGGTCAGCCTGGCAGCA650.10237993983209691No Hit
GCAAATGGGATAATGCATCTACTGTTAATGGTTTTGGTTAGTTTGATATTCA650.10237993983209691No Hit
TGACCCGGGTCTACATTCTCACGTTCTAGTGATTTGGGGCATGGGCTTAATA650.10237993983209691No Hit
GTATAGGGGAATCAGTCATCTCTCAGTTCAGTTTACCACCTCTGTTCATTTC650.10237993983209691No Hit
GGAAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC650.10237993983209691No Hit
ATTGCTGGGTCTGTGACACATTGATAACATTTTTAGGTCGGCTGGTAGCAGG650.10237993983209691No Hit
ACTACCGGGAAGATCTCAGTGGTGACAGGTCTAGCTTATTTCAAGAGCTGCA650.10237993983209691No Hit
ATCCGAGGGACCACTAATACTCTAGGAAAATGCTCTTAAAAAATTAATTTTT650.10237993983209691No Hit
AGCTATGGGAGTATTTAGAGGGTGAGAAGTGAATGTAACAGGTATAAATAAC650.10237993983209691No Hit
GCACCAGGAGTCTGTTGCCAGTAGTACACAGAATGAGCACTCAAATTATAAC650.10237993983209691No Hit
TGATGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA650.10237993983209691No Hit
GCCAAGGGAATTTAAGCTTTGGTCTCATCGTCTATAATGTGAGAGGAATTTA650.10237993983209691No Hit
AATCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA650.10237993983209691No Hit
TGCTCCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC640.10080486383468003No Hit
AACTGTGGGAGCCACTTTTTCCTTGGTCGTAGAGAGGTGTTACTCATATTAC640.10080486383468003No Hit
TGAGAGGGGGTAGATCAGAGCTCCAAATTTTGGATTATCGGGCCTGTGCAAG640.10080486383468003No Hit
GTTTACGGGTAAAGAGAATGGGTTTCCTCAGACTGTAGAGATAATAGTGGCA640.10080486383468003No Hit
ACTAAGGGGCACCCAGAAACAAGACTTTACAGCCTTCAATCCAATCAAGTTG640.10080486383468003No Hit
GCACTGGGGGAAAGAAATTTGCTAAATAAGACATGAAAACAAGACTGGGTAG640.10080486383468003No Hit
TTTGCAGGGTAGGTCAATTAATGTTAATTTTCATCTTCCATTAAATTCTTTT640.10080486383468003No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)