FastQCFastQC Report
Sat 31 Dec 2022
SRR5848157.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848157.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62679
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGCCGTCT3370.5376601413551588No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGC2490.3972622409419423No Hit
CCGTCTGGGAGCCTTCAGCAACCAAGGTTCTCCTGGGACCCAAAGTTTATGG1030.1643293607109239No Hit
AATGAAGGGTCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAG1020.16273393002441008No Hit
GTAAACGGGACATTGGACCAGGAAGGCTCAGAAAGGAGCCCAGGCCACAGAC940.1499704845322995No Hit
AGATTCGGGGTCCTCTAGAAAGAAAGCAAAAAAAGACCAATTTAGGTAGGAT940.1499704845322995No Hit
GAGTTAGGGGAAAAGTAGGTGTGAGGAGAGTAGCAAAACATGTTACTGAATA920.14677962315927184No Hit
CTTTGCGGGGTATTTACTCAAAACACAATTTGCACTGTACACTAGTGAATTG910.14518419247275802No Hit
GTGTCAGGGAGAGTATTATTTCAACATTTTCATTTTCATAAAAATTTTTTTA910.14518419247275802No Hit
TCCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA890.1419933310997304No Hit
AGCTCTGGGTCTTCACTGCTTTTTGCATTTTGAGTTGTGTGCCTATAAAATT890.1419933310997304No Hit
GGAAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC890.1419933310997304No Hit
ATCTTGGGGATTCGAAAACTGAAAAATAAGGCTAGTGTACTACAAAAGAGAA890.1419933310997304No Hit
CCAGCCGGGCACTGAATAAATGATAAATATATATTATCTTCCTTGTGATTTT890.1419933310997304No Hit
GGATGCGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGG880.14039790041321654No Hit
ATATGCGGGAACTTTGCAGAGAAAGAAGAAACAAGCTTGACTGCATTTTGTC880.14039790041321654No Hit
GTCCAAGGGAAATTCCAGGAAGTTGAAAGAATACAGACCACATCCTCAGATC860.13720703904018888No Hit
ACTGGCGGGGGTAAGATCAGAGCTGCAGGAGTCCAGACACGTACTTTGTCGC840.13401617766716126No Hit
AGTACTGGGAGAGCCCGCGCGCCGCCCGCTCCGGGTGCTGAATCCAGGCGTG840.13401617766716126No Hit
TTGGCAGGGAGAATGTACAGACTTTCAACCTCCCCAATACTGCTGTACCATC830.13242074698064743No Hit
GTTTAGGGGGAGGCGTGGCCGGAGCTGCGGCGGCAAGCGGGCTGGGACTGCT820.1308253162941336No Hit
GGAGATGGGATTATTGGCAAATTAATACATCAACACTTAAATCATTGACTAT820.1308253162941336No Hit
ATTGAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC820.1308253162941336No Hit
TTTGCAGGGGAAAAATATGAATAAAAATGAAAGGACAAACAAGAACCTATTA820.1308253162941336No Hit
AGCAAAGGAAATAATTAACAAAGTGAAGAGACAACCTACAGAACAGGAGAAA820.1308253162941336No Hit
GTCTAAGGGATATATGTTAAAGATCAGCAGAAAAATGGACAATGAAAAAGTG820.1308253162941336No Hit
GATCTACGGGAACCAATTTTATCCCCAATTCACAGGTGACAAAACTCAGGCT820.1308253162941336No Hit
TGCTAGGGGCGATCTCAGTATGGGGCTCCTTGGTATGCAATTAAATGACTTG810.12922988560761978No Hit
TCTCTGGGGAAGGGAAAGATAGTCTTTTCCAAAACAAATGCTGAAAGAATTT810.12922988560761978No Hit
TGCGCAGGGAGTAAATAAAAAGAAACGAAGTTGAAAGACTTGCTGGAGGAGG810.12922988560761978No Hit
CTAACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA810.12922988560761978No Hit
AATTTAGGGCGGCAATGTTGTATTTGCATTCTTTATCCCTAACTCTGAATCT800.12763445492110595No Hit
GCAAATGGGATAATGCATCTACTGTTAATGGTTTTGGTTAGTTTGATATTCA800.12763445492110595No Hit
GCAGTGGGGGTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAG800.12763445492110595No Hit
TATCACGGGATCACTGTGCAATTAAATTAGAACTCAAGATTGAAAGGTCCAC800.12763445492110595No Hit
AAGTCTGGGATTAATTGCCTATATGAAATTTACCAAATTAGACCAACAGTTA790.12603902423459212No Hit
TAGCCTGGGAATTGTAGTAAAATCAAGACCCCAAATTGCATAGAAATATTAT780.1244435935480783No Hit
GCTGTCGGGATGCATTGTGTTTTTTAAAAAGAGTATCTTATACATGTATCCT780.1244435935480783No Hit
TACTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA780.1244435935480783No Hit
GCATACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC780.1244435935480783No Hit
CGCTTTGGGGATCTGTGGTTGGGGAAAGCGATGGAGGAAAATTTACCAAGCA770.12284816286156448No Hit
GTCTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC770.12284816286156448No Hit
ATTGTGGGGAATACCATGATCGATGATGTCCCATGCTTCGAGGAAAACCAAA770.12284816286156448No Hit
AAACAGGGGGTTTAAAAATCTGTATGCATAAAGAATGCACCACTCAACTTTT770.12284816286156448No Hit
GCTCCCTTCCTGACCTCCCCTGACCCCGAAGATCACTACCTCTGTCATTCAG770.12284816286156448No Hit
CGGCTGGGGGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGTCACCGCGCC770.12284816286156448No Hit
TTTTGAGGGATGTGTTCCTTTTGCTTTGTAGTGTCTCCTGAGCAACCGCCTT760.12125273217505066No Hit
ACTAGGGGGGAACTACCCACAGCCTTCCTCCCCTCTTTGGGATGCGTCTGTA760.12125273217505066No Hit
TGATTGGGGACCCATCTCTGTGGAAGGCAGTTCAAAAAAAGTCAAGACTGAT760.12125273217505066No Hit
AATGCCGGGGTGTGTGTGGTGCACAGGTAAAGCTGGAGCTGGGAACTTTTTA760.12125273217505066No Hit
GGCTGCGGAGGTGGAGGTTCAAGACGGCCCCAATGAAGATGGGGAGATGTTC750.11965730148853684No Hit
AGACACGGGACATTCCATCCGTGGAAGAAATTGCAGCCAGCTTTAAGATTTT750.11965730148853684No Hit
TTACGCGGGATGACCTGGATGGTGATGGCAAGATCACCCGAGTGGAGATGCT750.11965730148853684No Hit
AATAATGGGATACATTGTAAATTAGTAACTATGGGTATTTTGTTTTAAATCT740.11806187080202302No Hit
GTTTCCGGGAAGCAACATTAAATAATTAGTTCTTGGACTGGTGTTTTTTCTT740.11806187080202302No Hit
ATTGCTGGGTCTGTGACACATTGATAACATTTTTAGGTCGGCTGGTAGCAGG740.11806187080202302No Hit
GTAAGCGGGATGGACCTCTCAGGAACCAGGAGCATGCAAGATTGCGGTGCTT730.11646644011550918No Hit
GTAAAGGGGGGACAAATTGAAGGTGTACGGCAAATGCACAGCAACGCACCAA730.11646644011550918No Hit
TGCAAAGGGTGGTAGTGGAAAATTCTCTCAGCATTTGTTTGTCTGAAAACGA730.11646644011550918No Hit
AAAGCAGGGATGTGATATATTTCCCCTCTTGAAGAGGCAATTCATTAAACTC730.11646644011550918No Hit
ATCGAAGGGGATTTAGGACAGAGGTAAGCAAATGGGCCAAATCTGGCCCACC720.11487100942899535No Hit
CTAGCTGGGAATGAGTGGTGTGGGTTTGCTCCTAGGGTTTCTTGTATGGCAG720.11487100942899535No Hit
TACTCAGGGTCGAGGAAGGAAGTAAGTACTTCTTAATTATTTTAAAAGAATA720.11487100942899535No Hit
ATGAATGGGGAAATGAGAATCTAGAATTTGTGAAAGGGCTGAATGTTCCTAT710.11327557874248152No Hit
TTCGATGGGTCTGGAGCTGGAGGCACCCCTCAAGATCTGCGGTGACATACAC710.11327557874248152No Hit
GAAATAGGGCGATCTTGTTGCGGGATACATTATCAATGCTGAAGCTGACAAC710.11327557874248152No Hit
GTTGGTGGGCATGAAGGATTTTCATTTCAGTGAAAGTCGCAGCAGAAGAGGG710.11327557874248152No Hit
ACCTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC710.11327557874248152No Hit
GTATTAGGGAGTCTGACTCCTATTCTCTTTACATTATTCCATGTGGCCTCTT710.11327557874248152No Hit
TGCAGGGACCTTATTAAAAGAAATAGTAATGAATCCACTTAATTATATTTCT710.11327557874248152No Hit
AGATCAGGGATGTACTATTTAACTTAAATATATTTTGTTTAATAGGAAATAT710.11327557874248152No Hit
CTGATAGGGAAGCTACATATCTCAGGGACCGAAATCAAGCCCAAAACTGTAC710.11327557874248152No Hit
AGCCAGGGGAGTGCGCAACCGTTCGCTAACTGAAATGATGGCGACTGGAACG710.11327557874248152No Hit
GATACTGGGGACTCAACACCCAAAAGTAATACTGAAAAACATGGACACATTT710.11327557874248152No Hit
AATTGCGGGGCATAGACACATGCTGATACCTGTGAGTTGCTTTCATCAATTA700.11168014805596771No Hit
TGAGAGGGGGTAGATCAGAGCTCCAAATTTTGGATTATCGGGCCTGTGCAAG700.11168014805596771No Hit
GTTCCCGGGACAGGAACGCTTGGTAACTGTGGATGAAATTGGAGAAGTGGAA700.11168014805596771No Hit
ATTTGAGGGGTGTCTGGGCATATTCGTCTGCTGATGGGGTTTGGGGAAGCCT700.11168014805596771No Hit
AAAGCAGGGTCTGGTATCGGGAGGAGAAATACTTTTCCATTGTTTAACTGCA700.11168014805596771No Hit
AAGTAAGGGTATATTAACTCATTTATTTTAGTTGCTGCTTTTTAGAACTTTC700.11168014805596771No Hit
ATTTTAGGGATAGTCATTGTAGGAAATGTCTTTTATAAGGAAATATTTCTTC690.11008471736945388No Hit
CACAAAGGGCTATGGTGCGTGCGGGCGCCGTGGGGGCTCATCTCCCCGCGTC690.11008471736945388No Hit
GTTGCTGGGAGTTTGAAAGCTTCTTTTAAAAAGAATTCCTCTAATATGACTT690.11008471736945388No Hit
GTCAGTGGGCAGGATGCTGATAGTCTCCAGCGGGCCTGGACTGTGGGCTGGT690.11008471736945388No Hit
GCTCCAGGGGAAACAGGCTGTCCTTCAAGCAGTCCGGTTATGTTACCCATCA680.10848928668294007No Hit
TGGCCCGGGCTTTGAGCAGAGAACAGATCCACCTTCAGGAGTTAGTGGTATG680.10848928668294007No Hit
TGAAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC680.10848928668294007No Hit
ACTAAGGGGCACCCAGAAACAAGACTTTACAGCCTTCAATCCAATCAAGTTG680.10848928668294007No Hit
CTGCAAGGGAGTTAAATCCACTTTCAGACACTTTAAAGATACCACCAAGAAT680.10848928668294007No Hit
CTGCAAGGGCTGGGTATTCCATGCAGCTAAAGGTCGAGGGCCAAGGTGGCTG680.10848928668294007No Hit
TATGTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA670.10689385599642624No Hit
GATCGAGGGGAGAGGAAGAGGAAGCTGCTGAGGACTGGGGAGAAGCTGAGCC670.10689385599642624No Hit
GGTCGTGGGATCCATCCAAATTAAAAATGTCTTCTCTGTGAAGGACCCCATT660.10529842530991242No Hit
CGAATAGGGCTTCTGACCTGCAGGACAGTTTTCCTGGAGCCATTTGGTATGA660.10529842530991242No Hit
CAGCCATGTTCCCGGGACCTCGGGAGCGTCCCCCACCGCCCGGTGCCATTCG660.10529842530991242No Hit
ACAGTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC660.10529842530991242No Hit
CAAAATGGGGCAGCTGCAGCTCATTACCAGTCTGAAGGAGCAAGCAATAGGC650.10370299462339858No Hit
TGCTTGGGGGGCTGGGGCTGGACGAGAGCGGCTTGGCCAAGCAGGCGGCCTC650.10370299462339858No Hit
CTAATCGGGGGATGTGCTGTGTTTTATGGATTATGTGTTTTATGTGCTGAAT650.10370299462339858No Hit
CACGTAGGGTATTATATGGCTATAGTATGAAAGACTGTGTAACATTAACAGA650.10370299462339858No Hit
TTACCAGGGGCACTTGGTATTTTGCAAAGTGCTTTTAAAACATTCTTACTTA650.10370299462339858No Hit
GGTCTAGGGAGAAAATATAGATGAGCAAAAAGTTGAAGATATACCAAATCCT650.10370299462339858No Hit
CGAGGTGGGGAGACCAAGGATAAAAGGATCGTTTTGTAAGACATGCCATGGC650.10370299462339858No Hit
GCTATGGGGAGCCACAGGCACATTCACAGGGCCCAAGGCAGGTGCAGCTGCA650.10370299462339858No Hit
CTGAGCGGGTGTTTTTTTCTGCTCAGAGATGCCTTGATCATCTTCCACTGCA650.10370299462339858No Hit
TTCCACGGGACCATTTGACTATAGTGTAAAAGTTTTTCCTTTACTTACTTGC640.10210756393688475No Hit
TCCTCTGGGTCTATCTAATTGTTTTTGTAGCAGCTGAAACTAAAATAATTTG640.10210756393688475No Hit
GTTTACGGGTAAAGAGAATGGGTTTCCTCAGACTGTAGAGATAATAGTGGCA640.10210756393688475No Hit
GGTTATGGGATAGATCCTTTTTTGTTCTCTGAGGTCATAATTTCCTCGGAGA640.10210756393688475No Hit
TACAATGGGGAAAATTTAGAAAACTGTTTTTCTCCTCAAGGAAGCACCAAAA640.10210756393688475No Hit
TACAGGGGCAACACTCCTGTGTAATCAGGTTCTCTGTCTTCTGGAATGATTT640.10210756393688475No Hit
GCATTAGGGGGGGCCAGCAGCACCCCGGGGTCTGCCCAGGGGAGTCAAGGCC640.10210756393688475No Hit
TGTTTAGGGTCTTGAGATTTGATTCTAAATATGGGAAGATGGTGGTTAGATT630.10051213325037094No Hit
GTCACGGGGGAATTCTAAGATTGCCTCATCTCAGCAGGTCACAGCTGAGATA630.10051213325037094No Hit
GCACAGGGGTATTTCTATGCATTAATTCTACATCTATGCAATGGGGATAATG630.10051213325037094No Hit
CGTATCGGGGTCAGACTTTTCTTTTTAAAAATTTAACATGATTGCTTTTCTC630.10051213325037094No Hit
TTTGCTGGGAGGGGGTAGTAAACTGAAACATAAAGTAAACATGCATACAAAT630.10051213325037094No Hit
GACCCAGGGGGACTCAACACCCAAAAGTAATACTGAAAAACATGGACACATT630.10051213325037094No Hit
TAAACAGGGTCAGAGAAAACAAGAAAGACTATTTGGGTTTATCACAAATCTT630.10051213325037094No Hit
ACATCCGGGACACAATTGAGGACTTCTGGCGAATGATCTGGGAGTGGAAATC630.10051213325037094No Hit
CATGACGGGGAATCTACTTTGCTGACATGTCTTCCAAGAGTGCCAATTACTG630.10051213325037094No Hit
ATATTAGGGCTCCAGGATAGAGTATATGTTAGGCTATAAAACTGTACTCAAT630.10051213325037094No Hit
GAACTAGGGAGGCTGCCATGACTGGGAGATGGTGGCACCTTCACAGAGACAG630.10051213325037094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)