FastQCFastQC Report
Sat 31 Dec 2022
SRR5848148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91407
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGCCGTCT4420.4835515879527826No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGC4400.4813635717177021No Hit
ACCTATGGGCTTCCGATCTGTATCTGGGAGGTGGAGATCACCTTATTCCTTT1530.1673832419836555No Hit
TGCTCTAGGGCAGAAAGAAGTGAGAAAGGCTTCTACTACTTCTTAAAATCTC1490.1630072095134946No Hit
CAACAGGGGCACCAAGAAGAATTTACCCAAAGAAGACTACCTCGAGATATCT1430.1564431608082532No Hit
GCAGCGGGGAGAAAATTTTCGCAACCTACTCATCTGACAAAGGGCTAATATC1410.15425514457317274No Hit
CACTACGGGAAGGAATTCAGCTTCGTGCAGCTCTCAGATGCCTACAGCACGG1400.1531611364556325No Hit
CATCATGGGAAACTGTAAATATTAAGAAATCCACATTGTCATTTCTTCTGTG1370.1498791121030118No Hit
TTATGAGGGACTTCCTTCCTGTGATTGGGTTGGTGGATGCTGAAAAGCTAAA1370.1498791121030118No Hit
GCCCTAGGGGTGGCAGGGTCCTATGCATATTCTTATTTTTTCTGTTGGATTG1370.1498791121030118No Hit
ATTCCCGGGTCTGACAGAGGGCTTTGAAAAAGAGGAAGGACACTTCTTTTAT1360.14878510398547157No Hit
TAACAAGGGACTCTGGCTTGCAGTAAGATAATTTCCAGTTTCCTCTGACGTT1360.14878510398547157No Hit
TAGGTGTGGGATAGGACCAAAAGAAACATAAAAGATATATCAATCAACCACA1350.14769109586793133No Hit
CACCACAGGGAATGCTTCAAAGATAAGAAACTTCATCCTAATGATTTCTAAA1350.14769109586793133No Hit
TGCTTAGGGGCTTTCTCCGCTGGCAACGGCGCCGCTCCCCGCTCCTCCTCCC1340.14659708775039113No Hit
TCTCGCGGGATACATATTATGAGAAATATTGACTAGCAAAAAGAAGAACGAG1320.14440907151531066No Hit
AAACTAGGGAAGTAAAAAGTTCATGTTCCTCACTATATCAGTTAGGAGTTAT1320.14440907151531066No Hit
TTTTACCGGGGAAGGAGGGAAAGAAGGAAGGAGGGAAAGAAGGAAGGAGGGA1290.14112704716268995No Hit
TAATGCGGGCGTGCCACTGCACTCCAGTCTGGGTGACAGAGTGAGACTCCGC1280.14003303904514972No Hit
GGGCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1260.13784502281006925No Hit
GAGCCAGGGCATTATTATAATCTAATACAAATTTTACTTTTTATTGTTTCCC1250.13675101469252904No Hit
TCAACCGGGCTGCCATGACTTCTTCTTTAGAAGGTTTCTCATTAAAAACATC1250.13675101469252904No Hit
GATCTTGGGGATGCGGGGGGCAGTGAGTTACCAGCTGGAGCGCTGTATCGTA1250.13675101469252904No Hit
AGACAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1250.13675101469252904No Hit
TGCGTTGGGTTGACTTTTGAATGCGGCAGTCAAGGGTTTAAGTGGGAAGCCC1240.13565700657498878No Hit
GACTCTGGGGCCCCAAGCCCTGGTGGTCTGGCCCTTTCTTTTTCCTCCTATC1230.13456299845744857No Hit
TTTTGTGGGGGAAGTTATGCTTATACAAGAGCAGAAGTTGTTTATGAATGGA1230.13456299845744857No Hit
TCCCCTGGGAGTGGACATGGTGAGATGGTCAAACTACTCTTGTCTAGAGGTG1230.13456299845744857No Hit
CGCCACGGATATCTTTTTCTGTTATTTTTCTTTCAAGCCATCTGTGTGGTTA1210.1323749822223681No Hit
ACTTTAGGGAATCCAACACTTTTTGGACATTATGAATCCAGCAATGGGGTGA1210.1323749822223681No Hit
TCAGATGGGAGAAACTGGCCATGGTATAATGGAAGAACCAGAACTTACTTTA1200.13128097410482786No Hit
AGCAGGGGGACTTAATACATTTTTTTTCAAGCGAAAGTGTCAGCAAAAAAAT1190.13018696598728763No Hit
ACTAGAGGGAAGATTGTTGATAGGAACTGTCTCAAAAGGATGGTGGTTATTT1190.13018696598728763No Hit
GTTGCAGGGAATCTATAAGCTGGAAGAACAGCTGCATAATGAAATGCAGTTA1190.13018696598728763No Hit
AGGAACGGGAAATGCAATATATGAACATTTACTATAAAAACATCTTTTAAAG1180.1290929578697474No Hit
CGGTGTGGGAAGAGGAGAAAGGCGTTGTCACCAATGGCCTAGACTTGAGCCC1180.1290929578697474No Hit
TGCCAAGGGAAAACAGGAAAATACATATTTCTAGATGGACATAAAACATATG1180.1290929578697474No Hit
CGGCACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1180.1290929578697474No Hit
GGTCACGGGATGTGGGGCATTTCCCTGGCCCAGCTCCTGGTGGACCCCAAGC1170.12799894975220716No Hit
CCAATAGGGGAACCTCAGAAACCAGTTTCAGCATATGCTTTATTCTTTCGTG1170.12799894975220716No Hit
GCCTATGGGGGGTATATTTGCTGCCAAGCCACTTGCCAAAGAAGAGGAACCA1160.12690494163466692No Hit
GCTTTAGGGAGTGTCCCCCACCAACGCGATCTGACTTGGGGAATGTTATTTA1150.1258109335171267No Hit
GCAAACGGGAAATAAAGATGGGACCCCGAGAGAAGGGACTAGGACTAAACGG1150.1258109335171267No Hit
ACGCTGGGGGATCTTGAAGTCGGGACTATCATAAATGAATATCCTACCAGTC1150.1258109335171267No Hit
TATTCTGGGCAGCTTTCAGAATAAAAACAATGATAAAAAAGACCAAGCACTA1150.1258109335171267No Hit
TAACATGGGAAGCTTGATTATAGTATTGTCACATTTTACCACAATACTAGAC1150.1258109335171267No Hit
ACGACTGGGAGCTTGTCTTGGCTTTTGACGTGTCCTCCTCACTAAGCTTAAT1140.12471692539958647No Hit
GCCGCTGGGCTCATCTAGGAAAAAAGGGCAGAAGTTAAGGTTTTCCTTAATT1130.12362291728204623No Hit
TCGGCTGGGATAGAAAAGCCTCCTGCTGAACGTCATATGATTTCTTCCTGGG1130.12362291728204623No Hit
CAGCATGGGACTTGATATTGTGCACATGCAGGCTGCGGCAGAGGGGAGTAGC1130.12362291728204623No Hit
TCACCAGGGGCTCCTCCCGCCGCCCAAGATGCCGAAAGGAAAGAAGGCCAAG1130.12362291728204623No Hit
TCAACCGGGTCTTAAGAGGAATAGCTTTTTTACTCAAGCAACCTTTGTTTAG1120.12252890916450601No Hit
AGTTTGGGGCGAGTTAGAAAAACGCAAGTTGTCTGGACTTGATGGTTTGATT1120.12252890916450601No Hit
TATAGTGGGATGTAACAAAGTCACTATTCATCAGAGTTTCATAGAATTATTA1120.12252890916450601No Hit
AGATCTGGGGAGATCGCTGATGGCTTGCAGTTGGCACGTGAAGGAAAGTGAG1110.12143490104696576No Hit
GGAATTTTTGCCATGTTGGAATAAATATCCAATATTAAATCTTTTAATTTCC1100.12034089292942553No Hit
GTTGCCGGGGGATTATGAGTATGCTGACTTTTCACGATTGGCTGGTGCATGT1100.12034089292942553No Hit
TTCCTTGGGACAGACTGGGTGGCTTCACTATGGAAATTTACTTTCTCACAGT1100.12034089292942553No Hit
TGACTAGGGAAGGTATTTTATAGTTTTAATGCAGTATTTTAAAGGTCATCTT1090.1192468848118853No Hit
TGATTGGGGACACTGGGGCTAGGGAAGTAGGTGATGCCAGCCCTCAAGTCTG1080.11815287669434506No Hit
CTTGCCGGGGACAAACACTTATATGATTCTCCTCTTTCCTGTTTACTGCAAA1080.11815287669434506No Hit
GAGCACGGGGGCGCGGCCCTTCGGGCGCCCGAGCCCGCAATGTCGGGCCCCA1080.11815287669434506No Hit
AAATTTGGGGGGAGGAATGAAAAGAAACATGGCAAATGAGGAACAGAAAATA1070.11705886857680484No Hit
AATCCAGGGGTATTAATGCAGTATACTGGGTAGACTTTTTCTTTCTGTATGG1070.11705886857680484No Hit
GCCAAGAGGATGTTTTGTTATGTATATGTGTCATTCTTCCCTTTTCTCTAAC1060.1159648604592646No Hit
TGAACTGGGAGTCATCTTGATGACAAGTGAATGCAAGGCAGCTGGTACCTTT1060.1159648604592646No Hit
GTCAGTGGGCATGGATGAAACTATAACTTAAAGAATAATACTTAGAAGGGTT1060.1159648604592646No Hit
GTGCCTGGGAACATCAACAAAAAGGATGACTACACAAAAACCCCACCCAAAG1050.11487085234172438No Hit
TGGTATGGGGGAGTACAGTTTACATCTTCACGGAAGTTTTTCACAATTTCTC1050.11487085234172438No Hit
GCATATGGGGAAAAACCCACAGCTAACATCATACTTAACTGTGAAAGACTGG1050.11487085234172438No Hit
TACTAAGGGCTTTAGAAAGTATTAGAAGCATGGTGATGGCTGCACGGCCTCC1050.11487085234172438No Hit
GTTACTGGGAAGGTATGTAACTTGAATAGGAAAAATGTCCATCAAATGCACC1050.11487085234172438No Hit
TAATTTGGGTCCTAGGGCAACCTGGGGCAGACAGGCCTGGTGGGGGGTGGGG1050.11487085234172438No Hit
TTGTTAGGGACCCTCTAACAGATTTTTAAGTGTATAATACAGTATTGCTGTC1040.11377684422418415No Hit
AGACAAGGGATTATGAATCACAATATCACAGTCACATATTTTAGCCTTCTGA1040.11377684422418415No Hit
ATGCAAGGGAAACACTCATCACATTTTTGTCATTGAATTATTTGCAATCAAG1040.11377684422418415No Hit
AAGGCTGGGATTAAAAAGCCAACCACTTATATTTTCCAAAGCCATTTGCATT1040.11377684422418415No Hit
TGTCTTGGGATCTGCCCCATTTTTAAAACGAGGTGGAATACCCAAGGTCATG1040.11377684422418415No Hit
CAGTCCGGGGACGGCAACCCCTGCAGCTCCTCTGCCTTTGTGCTCTGTTCCT1040.11377684422418415No Hit
GACAGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1030.11268283610664392No Hit
TACTGCGGGATCCTTGAGAGCTTTTAAGAAACCTTTAAAATTTAGTTGCTAT1030.11268283610664392No Hit
AACATCGGGCACAGAACTCCACCAGCAACTCCACCCGACCCCAGCAGTGGTG1020.11158882798910368No Hit
GGTACTGGGACGCTCTTCCGATCTGCTTCTGGGATTGCTGAAACACACAACC1020.11158882798910368No Hit
TTTGTTGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1020.11158882798910368No Hit
CGGGCGGGGAGACAAGATTTTCAGCAAGATGGATAAGAACAAAGATGACCAG1010.11049481987156345No Hit
TAGCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1010.11049481987156345No Hit
GCTGAAGGGACCCTCCCCCGCCACTGAAGGAAAGGGAGGCAAAGTGGGAATG1010.11049481987156345No Hit
CGCAATGGGCTATCTGTGCCATTTCCATGAGAAAAACATGTCTCTGCTAGCT1010.11049481987156345No Hit
TTTACTGGGACATATGCAAAGACATAGAAAATGATTTCTTTTCATCCAAGTG1000.10940081175402322No Hit
CGGCCTGGGCAATGTGTAATGCTGGGTAAAATAATTGTCTAATGCTGCAATG1000.10940081175402322No Hit
CTATGAAAGGGTGGAAAAGACAGACTTTTAAATTCTCAGGCGATGTATCTAC1000.10940081175402322No Hit
GGGATTGGGATGGAGAAGAAAAATGTCTTTTGTAATGCACTAGGCATGAGTA990.10830680363648297No Hit
CGGTCCGGGTAATTATATTGGGACTAGACCATAGCATTTTAAATTTCCAACT990.10830680363648297No Hit
TGAACAGGGCTATAATCAATGTCAACAATAACTGTGCTACTGGTTCTACTGC990.10830680363648297No Hit
CATACAGGGTGAATATTTCATGATTGTGTATCTCAAGTCTTTTCATTCATGG990.10830680363648297No Hit
GACACCGGGGCCCGCATGGTCATGCAGACCATGGAACCCAAGCAGCGGCCAG990.10830680363648297No Hit
CCAGGAGGGACCAAGAAAGAATGGTGGTTTGGGCCACATGAACATCGCACTC990.10830680363648297No Hit
TTTGCAGGGGAACTTGGATAGGTGATTAAATAGGGGGTGAGATCCCAGTTCC990.10830680363648297No Hit
ATGTTAGGGACAAAATTTTTCTGGAAAATAGACTCCTAACAGACCTACCAGA980.10721279551894275No Hit
TCATCAGGGATTCTTATTTTTACTTTAAAAGTCAGCACCTGAATGAAACCCA980.10721279551894275No Hit
GCAACTGGCCAGAAAGAGGCAATTATTTCCTCTCAGCAGCCTGGCCCCAGGA980.10721279551894275No Hit
TTTGCTGGGCGATCTAGTGCTCGGGATGGGGAATTCCTGAAAATTTTCTTCC980.10721279551894275No Hit
TCGTTAGGGCACAAAAGTGAAGCCAGTCCAAGAAGTGTCTCTTTTGTCCATC980.10721279551894275No Hit
TATCATGGGTAGATATTCTGTTTTATTTTGGTCATCTTTAGAAGTTATCAGG970.10611878740140251No Hit
GTGTGCGGGAGGAATCTCCATTTTTTAAACCATGTACAGGCAGTCTGTTTTC970.10611878740140251No Hit
ATAATGGGATACTTAGAGCTAAAAAATTTACAGAGGCTATGTAGTTCAGCTT970.10611878740140251No Hit
TAGCCAGGGGAGAAGAGGTTTTCTATGTCTTCAAGCACTATATCATAGTCCG970.10611878740140251No Hit
AGTCCGGGGAGCAGACACTTTTAAATGCTGTTTTCTCATTTAATACAGCAAA970.10611878740140251No Hit
GAGCAAGGGACTATCAGTTGGAAGGAGTAGCTATCTCTGCCAGAGTTTAAAA970.10611878740140251No Hit
TTGTTTGGGATTTGAATTACCAAGACGTCTGCTTTTGTGACAGTCAGAAAAC970.10611878740140251No Hit
ATTAAAGGGACTGGGAGCCAGAGATGGAACTTCAGGTCTTAAGTGCAAATCA960.1050247792838623No Hit
TAGTCCGGGGCAAGGCTGGTTCAACATACACAAATCAATAAACGTAATCCAT960.1050247792838623No Hit
TTAATGGGAGACATTACTATTCTCTATTCCAGTAAAAAGCCAACACTAAAAT960.1050247792838623No Hit
CATCATCGGGACTGTACTAGGAGTTATTATTCAGATTTATTTACCCACAAAA960.1050247792838623No Hit
CAAGAAGGGCATCGAAAGAGAACAGAAAGACCAAAAACCCTGAGGCTGGTTT960.1050247792838623No Hit
TAGCTAGGGCCTCCAGTCTACTGAAAATTGGCCAGGAGACAGATAAAACTAC950.10393077116632204No Hit
ACATGGGGGTCCAACTCATGAGTGGATTTTATATAGGATGGAACAGGAAGGT950.10393077116632204No Hit
CGACATGGAGAGTCATACTAATAAATGGCAGAACCAGGGATCAAATGAGACA950.10393077116632204No Hit
AATTGAGGGGAGTCAGGTGGAATCTTCCCCAACCTGGGCTTTGGTTTCATTT950.10393077116632204No Hit
AGTTCAGGGACACCAGCCTGTAGATGAGTTAGAAATGGGACTTTGTATCTTT950.10393077116632204No Hit
TCGACTCGGGATACATACTTTGATTCTTTAGTTACATAATTGATAACAAAGT940.10283676304878182No Hit
GGCTCTGGGGGGACGGGGCGCTCGCCCCACAGGCGCCAGGCGCTCCGGCCGC940.10283676304878182No Hit
GCGCAGGGAACTGCACCTTGGTTGTTGATGTCAAGTAAAAAAAAGATTGTTT940.10283676304878182No Hit
TGATGCTGGGATCATAAAAGGTTTATTAGACTAGACTTTAAAATGATGAATT940.10283676304878182No Hit
GTCTATGGGATAAAACATATGTATATATATTAGATAAACAGCAAACTTTGTT940.10283676304878182No Hit
GGAAACGGGATATTTCTTCCATTAAAATAAAAAATGGTCTCCCAATTCCCAA940.10283676304878182No Hit
CATGGTGGGACTTCCTATTATGTGTGCATAATATTCTCACACATTGCCTGAC930.10174275493124159No Hit
ATGAATGGGACTAATATGAGTATATACAAACTTGTAACCAGAAGAGGTAGAT930.10174275493124159No Hit
TAGATCGGGAGCTCGCTGCAACCTTCACCTCCTGGGTTCAAGCAATTCTCCT930.10174275493124159No Hit
GTACTAGGGCTACATTATCTTCTTTTTACTATTCTAGAAGATTCACTTTATA930.10174275493124159No Hit
GAAATGGGGAAACGATGAGATGAAATGAAAGGAAGAGATGAAATGTTGAGAT930.10174275493124159No Hit
CTATCTAGGGCTGAATTACAGTAGCAAACAGTTTGCAGAATCCTACCATTTC920.10064874681370137No Hit
CTTAATCAGGGGATGTCCAAAAACTGCTGTCATGGACTATTGATTGTATTGA920.10064874681370137No Hit
TTGTCGTGGGACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAA920.10064874681370137No Hit
CTGGACGGGAGATAGAGGCAACTCCCAGGCCAGTGAGCTTTACTTGCCGTGT920.10064874681370137No Hit
TTATACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA920.10064874681370137No Hit
CATACTGGGCATCCTCACAACTTGTGAGATGTGTATTCTGCATTTTTCAGAT920.10064874681370137No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)