FastQCFastQC Report
Sat 31 Dec 2022
SRR5848123.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848123.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66471
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGC5410.8138887635209339No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGCCGTCT5390.8108799326021874No Hit
GCGCGGGGGACTAATGACAGCTGGTGAGCTTTAAAAGCAAACAAACAAAAAA1460.21964465706849606No Hit
TTGCCCGGGATAAGCAGTTATTTTGGGAAACAGAAGCATTTAAAATCCATTT1240.1865475169622843No Hit
TTCTGCAGGGGGAGACGCGCACGCTCACGCAGTTCCACTTCCTCAGCTGGCC1160.17451219328729822No Hit
GATATAGGGCTGGAGCAGGAAGATAAAATGAGCTTGAGCTCTGGCAAGCCCA1100.16548570053105865No Hit
AAAACTGGGTGAAATTAATGAAATTAGTGCAGTGTCTCTCTCTCTTTTTATG1090.1639812850716854No Hit
AGAATGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1090.1639812850716854No Hit
GAAATAGGGAGATAAATGAAACAAAGAGCTGGTTCTTTGAAAAGATAAACAA1080.16247686961231214No Hit
GGCCCCCCATGGACCTGCAGCCGCAATCCTCAGATGAGCAAGTGCCGCTGCA1070.16097245415293887No Hit
ATGCAGGGGTTCTAGCCAGTGCAGTAAGGCAAGGAAAAACAATAAAAGGCTT1060.1594680386935656No Hit
GAACTGGGGTGATGCACACATTTTCAATGTGCTACTGTATGACATATTTATA1060.1594680386935656No Hit
CCACATGGGTGTGAAGAGTCTGCTTAAACATTTAACAGAGATGGGCTCACTT1050.15796362323419236No Hit
GCAGACGGGCTACAGATGGGAGAAAATTTTTGCAAACTAGGCATCTGACACA1040.1564592077748191No Hit
TTAAAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1030.15495479231544584No Hit
TTCGCTGGGTGGATTCTGAAAGTTAAAAATTTAGTTGTTCTCCCCAATGTGT1010.1519459613966993No Hit
TGTCTCTATTAAAAAAGAAAAAAGAAGAAGAAAATTAGTAAGGAAATAGAAC990.1489371304779528No Hit
AAACATGGGTATCAGTATTAAGTAGACAGCATATTATTCAAGTGTTTAGACT990.1489371304779528No Hit
GTAACTGGGGGTTATGTTCCTCATCCTGGCTCAGTTACTGAGTTTCCTGTGT980.14743271501857952No Hit
AACATAGGGCTGACTTGCTTTGAAGTCTGTTATTTTAATCACTCCAACATGA970.14592829955920628No Hit
GGTAATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA940.1414150531810865No Hit
TCTTCCGGGATGAGAAGAGCTCTCGTTTCATGAAAGTTGGCTATGAGAGAGA920.13840622226233998No Hit
TAGCTAGGGCATCAAAAAATAGAAAGATCACAAGTTAACAAGCTAACAATGT910.1369018068029667No Hit
TCTCAGGGGAGAACAAAAGCAATACTGAATTTCCCTTTCAACTCAAAGGCCA910.1369018068029667No Hit
TGAAGCGGGGTGTACAAATGACATCAAATATAAAAATAATTACAGGATGTTC910.1369018068029667No Hit
GTTACTGGGGCTTTCATTCTAACATTCAGACATAGCAATCCAAACCCTTGTT900.13539739134359344No Hit
GGATTTGGGTCATGGCTGAATAACAGTATCGAAATGTTATCATAATTCTTTG890.1338929758842202No Hit
CATTAGGGGAACTCAGTAGAAGGGCTTAAGAACAGATTTGGCCTAGCATGAA880.13238856042484692No Hit
CGATATGGGAGTGCTACAAAGGACAGTCGAAGCTAGGAAGTGCTCAGCAGAG880.13238856042484692No Hit
GCTATAGGGATGGGAGTTCTTTCATGGCCTCTGCTTGGGGTGGGGGAGTCAG880.13238856042484692No Hit
TTAGCTGGGGATTCTGGGTCATCAGAAGTTCATAAAACACTAAATTAAGGTT870.13088414496547365No Hit
CGTCTCGGGGCCTCACTTAAAAGCTAAACCCAGCATTTGTTACAGGCCTTTT870.13088414496547365No Hit
TCTGCGGGGAACTCAATTACAAGAAGATCTCTGAAAAAACTTACAATTACAT870.13088414496547365No Hit
TACGGTGGGTTACTGATGCTTGGTGTATATTTTGATTAATTTAGAGACATTT860.1293797295061004No Hit
TTAAATGGGTAACACAAAGCTGCAGGTTTTAATTTTGCTTTGTCAATATGTT860.1293797295061004No Hit
TCTTAAGGGTCTGCTTTCCTAAAGCTATGCAGGGCTCCCCAAGCAATTGGAA850.12787531404672714No Hit
AGACGCGGGATGAGCACTGAGCCATAAAAGGTTTTCAGCAAGTTGTAACTTA850.12787531404672714No Hit
TATGTGGTAAGGATGATTAGACATCTCACTACTACTACTGACTATCACACAC840.1263708985873539No Hit
CTCACCGGGGAGAAGTCAAGCTTGAAGTAGAAAATGTCATGATGAGAAAAAT820.12336206766860737No Hit
TCTCACGGGTTGATGGAAGTTGGAAGAGAACCATGGGAGTTCACTGTATAGA820.12336206766860737No Hit
GATCTAGGGTCCGATTTAACTGTGGGGCATTCAAATGTGTTCATGTATTGTA820.12336206766860737No Hit
TTGCGCGCGGGTCAGCCTTCTGGCAGACTTGGAAGTACTCTTGAGAAAAGAA810.1218576522092341No Hit
GACGCGGTATGACAGAATTTGGTGCACAGAAATATAGAACACAAAACCAAAT810.1218576522092341No Hit
GTTATAGGGGGAAATGGAACTGAGGGACCGGTGACACGTGCTTCAGACCGGT800.12035323674986084No Hit
AGCAGTGGGGAAAAACTGCAGTATCTAGTCACTTTTCTGAATGTCTGGGCTA800.12035323674986084No Hit
TCATAAGGGCTGCAGATGGGGGCCCGGGAGGCACACACGAGTCTGGGACTCA790.11884882129048757No Hit
GCGTCAGGGAACACCCACTGTTATATTTGGGAAGGAGCCTTGGCAGTAACAT790.11884882129048757No Hit
GTAATTGGGTGGCTAGTTGCATTTGTAAATAAGTATAATGTTGCTTTGATTT790.11884882129048757No Hit
CCTGCTGGGTGGTATTCCACAGGAAACAATTTCTGGAAACAAGAATTATTTG790.11884882129048757No Hit
GCCATGGGTGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATT790.11884882129048757No Hit
ACATAGGGGGAATATCTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.11734440583111433No Hit
GAGCATGGGGAGTGTGCAGGCTTAGAGGGACTAGGCTGGGTGTGGAGCTGCA780.11734440583111433No Hit
ATCGACGGGCTTTCTATGTCAATAAATGTGTCATAAGATATCTATAAATATT780.11734440583111433No Hit
AATCGTGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG780.11734440583111433No Hit
ACTTTAGGGTGTGCTAGGAAGTGTTAAGTGCTTTATAGATATTATTTCCCTC770.11583999037174106No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCC770.11583999037174106No Hit
CGAGCCGGGCATGGGCATAACGCAGAATACGCACTTAAGTAGGTGTGTGCAC770.11583999037174106No Hit
GTTCCCGGGAGATGAAAAGAACCCTTTAAAAGAGTGCCTTCCACATAGCCAT770.11583999037174106No Hit
AGTGCCGGGTCTATCTGCCATGATGTGATATGATTTATAATGATTTAAACAG770.11583999037174106No Hit
TTCGCGGGGTTCCAGGGCTCTCTGTCTGCCTCACTACAGCTACATTGAGGAT760.1143355749123678No Hit
CTGTGCCGGGAACCAGTTTTTATGTTCAGCTGATTCTAAATGAATCTATTCC760.1143355749123678No Hit
GCTACGGGGAGAAGTGAGGCATGTCTTACATGGTGGCAGGTGAGAAAGAGGA760.1143355749123678No Hit
GCGCCCGGGTTGCTAATATATGAGGTAATGCATATGTTAACTAACTTAATGA760.1143355749123678No Hit
GCTTCAGGGGAGATACAGAAACCGACAGGGGCCAGGCGCCCGGTGGCTCCGA750.11283115945299453No Hit
TAGTCTCGGGAAGGCCAAATAAGCATACATGCATCCATACTGATAACATAGG750.11283115945299453No Hit
TACTTGGGGTGTCTTATCCACCTTTAGGATTGGAGAGGGAGAAAGAAAAACT750.11283115945299453No Hit
ATTTTTGGGTCATAAATTCACTGAAGTTATCTTTGAAGTTTGTTTCCAACTT750.11283115945299453No Hit
TTCTGCGGGAAGATCAACAAATCCAGAAATCGGTTCTTTGAAAGAAATTAAT740.11132674399362127No Hit
GTTAGGGTGAATGGAGTAGTAACATGTATAGATAGGTGACTTTTTTAGGATA740.11132674399362127No Hit
GCAAACGGGGTGTAGGTAAGTGGGGCCCTAGCTAGGTCTGGGAAGATGGTCA730.10982232853424803No Hit
TCATAGGGGTCTTCAACAACATTGCCTACCGCAGCTCCTCAGCCAACCGCCT730.10982232853424803No Hit
ACACATGGGAATGTGAGCCTGAGAGAGAGTGGGAAAAGTATAGAGTGAGTAT730.10982232853424803No Hit
AGCTTGGGGCTGGGGCAATAATCAGGACGGAGTGGTGGCCAGCCAGCCTGCT730.10982232853424803No Hit
CTAAATGGGTGCCTCCCGGGTTCAAGCAATTCTCTGCCTCAGCCTCCCAAGT730.10982232853424803No Hit
CGTCTGGGGAGGCCTCTGAAGAGAAACTGCCTTTTCCCTCTTTCAGCCAAAA730.10982232853424803No Hit
GCTAGTGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA720.10831791307487476No Hit
CTGATAGGGATCTCAGCTCACTGCAGCCTCCGCCTCCCAGGTTCAAGCGATT720.10831791307487476No Hit
ACTACTGGGACCCTTGAACAACACGGGTTTGAACTGTGTGGATCCACTTATA720.10831791307487476No Hit
CAAAATGGGGAATTTGTGGATCTTGGTATAAAGTCATTGGAACATATCTGTT720.10831791307487476No Hit
ACAGCGGGGGTTTTGACTGTCTTTTTGCTCTTTCCTATATAATGAATAGATG720.10831791307487476No Hit
AGTCCAGGGTATTCTACCTATAAAATTGCCTGTCGTTAACAATTCAGTAACA710.1068134976155015No Hit
TACGCCGGGTATTACTTCTGCAGTGCGACATCCAGAAATGAGCTGATGTCAA710.1068134976155015No Hit
TTAACCGGGAGTTTTTGAGGAGGAGAGACAACTAAGTTCAATTAACACCACT710.1068134976155015No Hit
CAAGCGGGTATTGGGATGGCATCACCTTCTGTACTATGCAGCAGAAGGAGAC710.1068134976155015No Hit
TAACGTAGGGTGGAAAAAAGATACTCACACAGAACAAAACAGTTCTTGGTCT710.1068134976155015No Hit
ATTGCTGGGTCTGTGAAGGGGTGCTTTGAACAAAAACCAGCCTAGTGAATTG710.1068134976155015No Hit
CATACAGGGTATCATCTATGCCGGTGAGAAGGCCCAGTTTGCACAGCCGGAG710.1068134976155015No Hit
TCTCGTGGGTCGTAAGCTGGTGAAAAAAAGAATAAACTTTGCCTCTCTGATA700.10530908215612823No Hit
ATGAGCGGGTGATGTAAAATCCAGGCTATCAGAGCTGGGTCACAATTTAGTG700.10530908215612823No Hit
GGTTTTGGGGTGAGAACATCTTCTGTCCAGCTGTACATGCTGAGGTGACTGT700.10530908215612823No Hit
TATGGTGGGTACTGATGGATTAAGCAGATAATTTTTATTCCTGCCTTACTTT700.10530908215612823No Hit
AGCCAAGGGACCAGTTTCTAATGGAAATTAGAATTCTAATTGAATATTGTTT700.10530908215612823No Hit
TGTACTGGGTTATCTAAAACAATTTCTTCAAAGGAAACAAAAAATGAGATTT700.10530908215612823No Hit
GACAATGGGAAGGGTAATGTAGCAGAAGACAGGATGGGTTTCTCTTGCCTGA700.10530908215612823No Hit
ACTGCATGGGAATAAAGGAATGGAAAAAGATATTTCATGCTAAAAGAAACCA700.10530908215612823No Hit
TGCGTTCGGGTGAAAGGAGGGTAAAAAGACTAAAATGAGGGGGAACAGAGTT700.10530908215612823No Hit
TGAGTTGGGCACAGTGAACGTAACTGTAACATATATGAAAGGCTTCTGGGAC690.10380466669675498No Hit
GTTAATGGGTTACTAATTGACTTTAAAAGTTAAAAAGTAAATAATGAGTAAT690.10380466669675498No Hit
TGTTACGGGAATTCCCTACCATATGTGCTTGTTACATTTTCAATATGAAAAG690.10380466669675498No Hit
ATAACAGGGTGATATCTGAGCATGATATTTGGAGAGGCCATATATCCAAACT690.10380466669675498No Hit
TTGACTGGGTACTGCGTATTGAAGAAAAAAATCTGACACCACCGAGCCAAAG690.10380466669675498No Hit
ACTGCAGGAAGGAATTGTTATATTGTAGAAAAGTTTATCTTAAAAAAATCTT680.1023002512373817No Hit
AAGCATGGGAATGAAGCCAGGCATGAAAAGACAAAGGAGTTTATACAGAGAA680.1023002512373817No Hit
TCTAGTAGGGTAGAACATAAAACATGTTTTTATTCAAGTTAGTTAATATGAT680.1023002512373817No Hit
CGCTGCGGGTCACCTTGTAACCTGAGTTACAAATAGACCTTTTCTTTCAGAA680.1023002512373817No Hit
AGGCTGGGGCAAAATACTCCAGGAGAGAAGCATTGCTTATATCAACTCGGAT680.1023002512373817No Hit
GGCTATGGGAACTCCCTGGATTCAAGTAGCACATTGGTAATAGGTATCAGAG680.1023002512373817No Hit
AGTAGAGGGGCTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCA680.1023002512373817No Hit
GCTACAGGGAATTACTGTTTAGCAGTGCTTTTTTATTTGAGTATTTTGAAAT680.1023002512373817No Hit
TATCATGGGTGAAACAACCTTGCCAGTGGCAGAGCATTTCTATAACACTGGT670.10079583577800846No Hit
TTGCACGGGGTCTGATGGCGGGGAACACAAAGAGGGACAGAAAAAAAAGAAG670.10079583577800846No Hit
ATTGACGGGTGCAACCTCCGGGTTCAAGAGTTTCTCTTGCCTCAGCCTCCCA670.10079583577800846No Hit
ACAACCGGGGAGAAGGTGAGCCAATGTAAATAGACACACACATAAAATCAAG670.10079583577800846No Hit
GTTACAGGGTGGTGTTAGTTCTTCTTTAAATGTTTGGTAGAATTCATCAGTG670.10079583577800846No Hit
CCAATTGGGGATACAGGAAGGCACTGGCTTACTCGGGTGCTCTTACCTTCTA670.10079583577800846No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)