FastQCFastQC Report
Sat 31 Dec 2022
SRR5848122.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848122.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66363
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGC5380.8106927052725162No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGCCGTCT5220.7865828850413634No Hit
GCGCGGGGGACTAATGACAGCTGGTGAGCTTTAAAAGCAAACAAACAAAAAA1390.20945406325814084No Hit
GATATAGGGCTGGAGCAGGAAGATAAAATGAGCTTGAGCTCTGGCAAGCCCA1360.20493347196479964No Hit
AAAACTGGGTGAAATTAATGAAATTAGTGCAGTGTCTCTCTCTCTTTTTATG1240.186851106791435No Hit
TAGCTAGGGCATCAAAAAATAGAAAGATCACAAGTTAACAAGCTAACAATGT1190.1793167879691997No Hit
TTGCCCGGGATAAGCAGTTATTTTGGGAAACAGAAGCATTTAAAATCCATTT1180.17780992420475267No Hit
GCAGACGGGCTACAGATGGGAGAAAATTTTTGCAAACTAGGCATCTGACACA1110.16726187785362326No Hit
CCACATGGGTGTGAAGAGTCTGCTTAAACATTTAACAGAGATGGGCTCACTT1080.16274128656028208No Hit
AGTCCAGGGTATTCTACCTATAAAATTGCCTGTCGTTAACAATTCAGTAACA1060.15972755903138797No Hit
ATGCAGGGGTTCTAGCCAGTGCAGTAAGGCAAGGAAAAACAATAAAAGGCTT1050.1582206952669409No Hit
TTCTGCAGGGGGAGACGCGCACGCTCACGCAGTTCCACTTCCTCAGCTGGCC1020.15370010397359976No Hit
GCTAGTGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1010.1521932402091527No Hit
GAAATAGGGAGATAAATGAAACAAAGAGCTGGTTCTTTGAAAAGATAAACAA1010.1521932402091527No Hit
GTTACTGGGGCTTTCATTCTAACATTCAGACATAGCAATCCAAACCCTTGTT1010.1521932402091527No Hit
TCATAGGGGTCTTCAACAACATTGCCTACCGCAGCTCCTCAGCCAACCGCCT1000.15068637644470564No Hit
AAACATGGGTATCAGTATTAAGTAGACAGCATATTATTCAAGTGTTTAGACT1000.15068637644470564No Hit
GTAACTGGGGGTTATGTTCCTCATCCTGGCTCAGTTACTGAGTTTCCTGTGT990.14917951268025859No Hit
TCTTCCGGGATGAGAAGAGCTCTCGTTTCATGAAAGTTGGCTATGAGAGAGA990.14917951268025859No Hit
TACAACGGGTGATTACTGCAGAGCAGCGTTAATGGAGTGCTTACTTTACATG980.14767264891581153No Hit
GAACTGGGGTGATGCACACATTTTCAATGTGCTACTGTATGACATATTTATA970.14616578515136447No Hit
AGCTTGGGGCTGGGGCAATAATCAGGACGGAGTGGTGGCCAGCCAGCCTGCT970.14616578515136447No Hit
TGTCTCTATTAAAAAAGAAAAAAGAAGAAGAAAATTAGTAAGGAAATAGAAC960.1446589213869174No Hit
GAGCATGGGGAGTGTGCAGGCTTAGAGGGACTAGGCTGGGTGTGGAGCTGCA960.1446589213869174No Hit
TCTGCGGGGAACTCAATTACAAGAAGATCTCTGAAAAAACTTACAATTACAT960.1446589213869174No Hit
GGTAATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA950.14315205762247035No Hit
AGAATGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA950.14315205762247035No Hit
GACTAGGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG950.14315205762247035No Hit
TCATAAGGGCTGCAGATGGGGGCCCGGGAGGCACACACGAGTCTGGGACTCA940.1416451938580233No Hit
TCTCAGGGGAGAACAAAAGCAATACTGAATTTCCCTTTCAACTCAAAGGCCA940.1416451938580233No Hit
ACATAGGGGGAATATCTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.14013833009357624No Hit
CATACAGGGTATCATCTATGCCGGTGAGAAGGCCCAGTTTGCACAGCCGGAG930.14013833009357624No Hit
GGATGCGGGCAAGGGTGACGTGTTTGTGGCCCCCCGGACAGACCACCCAGCC920.1386314663291292No Hit
TCTCTTGGGGGTCTTTTCCAGACATTTCATATAAATGGAAACCTTACAATAT920.1386314663291292No Hit
GGCCCCCCATGGACCTGCAGCCGCAATCCTCAGATGAGCAAGTGCCGCTGCA900.1356177388002351No Hit
TCTTAAGGGTCTGCTTTCCTAAAGCTATGCAGGGCTCCCCAAGCAATTGGAA900.1356177388002351No Hit
AGACGCGGGATGAGCACTGAGCCATAAAAGGTTTTCAGCAAGTTGTAACTTA900.1356177388002351No Hit
CTGATAGGGATCTCAGCTCACTGCAGCCTCCGCCTCCCAGGTTCAAGCGATT890.134110875035788No Hit
TTCGCTGGGTGGATTCTGAAAGTTAAAAATTTAGTTGTTCTCCCCAATGTGT890.134110875035788No Hit
GTTGTGGGACGGCCTACCTGGAATTTAAAATTCAGTAGACAAATTAATTGGT880.13260401127134097No Hit
TTAGCTGGGGATTCTGGGTCATCAGAAGTTCATAAAACACTAAATTAAGGTT880.13260401127134097No Hit
TACGGTGGGTTACTGATGCTTGGTGTATATTTTGATTAATTTAGAGACATTT880.13260401127134097No Hit
ATGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAA870.1310971475068939No Hit
CCAGGGGATAGAACTAAAACATGAAAAGTAACTACTACAAAAGCACTGTAGA860.12959028374244683No Hit
ATACCGGGGATCAAATATTTAGAAAAAAAGTACAACTTATATATACTTTTTC860.12959028374244683No Hit
GACGTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC860.12959028374244683No Hit
GATCTAGGGTCCGATTTAACTGTGGGGCATTCAAATGTGTTCATGTATTGTA860.12959028374244683No Hit
CGATATGGGAGTGCTACAAAGGACAGTCGAAGCTAGGAAGTGCTCAGCAGAG840.1265765562135527No Hit
CATTAGGGGAACTCAGTAGAAGGGCTTAAGAACAGATTTGGCCTAGCATGAA830.12506969244910568No Hit
CAAAATGGGGAATTTGTGGATCTTGGTATAAAGTCATTGGAACATATCTGTT830.12506969244910568No Hit
TCTAATGGGAGGCTTTGAGAGTGTTTGAAGTAACTAATAGAAAAAGAAATTG830.12506969244910568No Hit
CTGCCAGGGACCGAACCCATCTACATGCTCAATTGGATCATACGGTTGCAGG830.12506969244910568No Hit
GCCATGGGTGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATT830.12506969244910568No Hit
TTCTGCGGGAAGATCAACAAATCCAGAAATCGGTTCTTTGAAAGAAATTAAT820.12356282868465861No Hit
GCTATAGGGATGGGAGTTCTTTCATGGCCTCTGCTTGGGGTGGGGGAGTCAG820.12356282868465861No Hit
TAACGTAGGGTGGAAAAAAGATACTCACACAGAACAAAACAGTTCTTGGTCT810.12205596492021156No Hit
TGAAGCGGGGTGTACAAATGACATCAAATATAAAAATAATTACAGGATGTTC800.1205491011557645No Hit
GCTCAGGGGATCACGAATTACAGATGACATGATCCTTTATGTAGAAAACCCC800.1205491011557645No Hit
GACAATGGGAAGGGTAATGTAGCAGAAGACAGGATGGGTTTCTCTTGCCTGA800.1205491011557645No Hit
ATGAGCGGGTGATGTAAAATCCAGGCTATCAGAGCTGGGTCACAATTTAGTG790.11904223739131745No Hit
AGACGGGAATAAGCTGCAATTTTGTACAGATACCAACTTCTGAAAAGCTGGT790.11904223739131745No Hit
TTAAAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC790.11904223739131745No Hit
TGATGAGGGAGCGAGGCGGCCGCAGGTACCGCAAGATGAAGGAGCGGCTGGG790.11904223739131745No Hit
GTGCAGGGGACAGGCATCTATGGGATGTGGAATCTGTATGTCTTTGCTCTGA790.11904223739131745No Hit
AATTGTGGGTGAATGTTCTTAATATGATTTGGTACCTAAAGTAATTGAAAAA780.11753537362687039No Hit
AGCAGTGGGGAAAAACTGCAGTATCTAGTCACTTTTCTGAATGTCTGGGCTA780.11753537362687039No Hit
CTCACCGGGGAGAAGTCAAGCTTGAAGTAGAAAATGTCATGATGAGAAAAAT780.11753537362687039No Hit
GGATTTGGGTCATGGCTGAATAACAGTATCGAAATGTTATCATAATTCTTTG770.11602850986242334No Hit
GATTATGGGGTATATTAATTATTCTAAAAGGGCTTTGTTCCTTTCCATTTTC770.11602850986242334No Hit
ACACATGGGAATGTGAGCCTGAGAGAGAGTGGGAAAAGTATAGAGTGAGTAT770.11602850986242334No Hit
CGAAATGGGTGTGATCCTTCTTCTGAAAATTCAGTGGCTGGCCTCCAGACCT770.11602850986242334No Hit
TGATAGGGAAGCAAACCAGAAGCCAGTGGATTTCTAGACAGAACCTACCTTA770.11602850986242334No Hit
TTCGCGGGGTTCCAGGGCTCTCTGTCTGCCTCACTACAGCTACATTGAGGAT760.11452164609797627No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCC760.11452164609797627No Hit
GTTAATGGGTTACTAATTGACTTTAAAAGTTAAAAAGTAAATAATGAGTAAT760.11452164609797627No Hit
AACATAGGGCTGACTTGCTTTGAAGTCTGTTATTTTAATCACTCCAACATGA760.11452164609797627No Hit
TTGCGCGCGGGTCAGCCTTCTGGCAGACTTGGAAGTACTCTTGAGAAAAGAA750.11301478233352923No Hit
TTTGCAGGGCATGCGGTGACTGAACAGGCAATACAGTACGGTACTGAGGATA750.11301478233352923No Hit
ATTGACGGGTGCAACCTCCGGGTTCAAGAGTTTCTCTTGCCTCAGCCTCCCA750.11301478233352923No Hit
AGTGCCGGGTCTATCTGCCATGATGTGATATGATTTATAATGATTTAAACAG750.11301478233352923No Hit
CAAAGCGGGTTCTTTTCCCAGGGCTGTTCCTTGCTGAGAAAAATAATTCAGT750.11301478233352923No Hit
GAAAAAGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT750.11301478233352923No Hit
GCTCTGGGGTATCTAAATGAGAAGCAGAGAAATAAAGTCAAGAAAATTTACC740.11150791856908215No Hit
ACAACCGGGGAGAAGGTGAGCCAATGTAAATAGACACACACATAAAATCAAG740.11150791856908215No Hit
CAAGCGGGTATTGGGATGGCATCACCTTCTGTACTATGCAGCAGAAGGAGAC740.11150791856908215No Hit
CATCTAGGGATAAAACACTGAGTGCTGTTTTCACAGTATCAATTAGAGAATT730.11000105480463511No Hit
ATGTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA730.11000105480463511No Hit
ACCAGCGGGCTGAGTCCCTTATGATACAGCCTGAATGCATCATGACAGATCC730.11000105480463511No Hit
GCTACAGGGAATTACTGTTTAGCAGTGCTTTTTTATTTGAGTATTTTGAAAT730.11000105480463511No Hit
TTAACCGGGAGTTTTTGAGGAGGAGAGACAACTAAGTTCAATTAACACCACT730.11000105480463511No Hit
TTGACTGGGTACTGCGTATTGAAGAAAAAAATCTGACACCACCGAGCCAAAG730.11000105480463511No Hit
CTAAGTGGGTAATTTGTTTTTAAAACAACTAGATCAGCTTGTTGTTTATTAG730.11000105480463511No Hit
ACTGCATGGGAATAAAGGAATGGAAAAAGATATTTCATGCTAAAAGAAACCA730.11000105480463511No Hit
TGCGTTCGGGTGAAAGGAGGGTAAAAAGACTAAAATGAGGGGGAACAGAGTT730.11000105480463511No Hit
TGCTCCGGGGTCTACTTGCAAGGGGGCCTGGAAAATAAGGTTTCCAGCTGGT720.10849419104018805No Hit
ACTTTAGGGTGTGCTAGGAAGTGTTAAGTGCTTTATAGATATTATTTCCCTC710.106987327275741No Hit
TTGCTGGGGGGGATCCCGCTCCCGCACCGCCATCATGATCTGCCTGGTGCTG710.106987327275741No Hit
TTCAGCGGGCAGCCTCACAGAGCTTCCCTGAGCCTCACCTCTGAAAGAGATG710.106987327275741No Hit
ACTACTGGGACCCTTGAACAACACGGGTTTGAACTGTGTGGATCCACTTATA710.106987327275741No Hit
TGAACAGGGATTCTTCAGATTGATATCTTTAAAGTGCCACCATTTCTCTTTT710.106987327275741No Hit
ACAGTAGGGAGGTCAGAGAGTGGTTTGCAGAAAGACAGAGAAGATCAGAATT710.106987327275741No Hit
GAAACAGGGAGAGCAATAAAAGCTCAAATTCCAAGTGTAACTTCTGTATTTG710.106987327275741No Hit
GCTACGGGGAGAAGTGAGGCATGTCTTACATGGTGGCAGGTGAGAAAGAGGA710.106987327275741No Hit
ATTGCTGGGTCTGTGAAGGGGTGCTTTGAACAAAAACCAGCCTAGTGAATTG710.106987327275741No Hit
ATGCGAAGGGTCATAGATAGTCATTTTGCCTCCTGGACAGTTGGCTGACGAA710.106987327275741No Hit
TCGATAGGGTACATGGGGCAGCCTAAACGGGGAGGAGGAGCTGTTTCCTTTG700.10548046351129393No Hit
GTCCTTGGGTATATAACATAAAACTCACCAGTATCAAGTGCACGATTTGAAG700.10548046351129393No Hit
GTACAAGGGTGATGCCACTTTCACTGTAGGTGGAAATAATTCTCCCTCCTTC700.10548046351129393No Hit
CGCTGCGGGTCACCTTGTAACCTGAGTTACAAATAGACCTTTTCTTTCAGAA700.10548046351129393No Hit
CTTAATGGGATGCATTCTCAGATTATGCCAACTTCAAGTAAGCGGCAACAAG700.10548046351129393No Hit
TAGTCTCGGGAAGGCCAAATAAGCATACATGCATCCATACTGATAACATAGG700.10548046351129393No Hit
CGTCTGGGGAGGCCTCTGAAGAGAAACTGCCTTTTCCCTCTTTCAGCCAAAA700.10548046351129393No Hit
TCTCACGGGTTGATGGAAGTTGGAAGAGAACCATGGGAGTTCACTGTATAGA700.10548046351129393No Hit
AAAACTGGGAAGGCTCAACTTTATATGCATACAATGACATATTATACAGTCT700.10548046351129393No Hit
GAAATCGGGTACTTGGAGAAACTAGGTGAAGATGCAACCAGAAAGGAGAATA700.10548046351129393No Hit
TTAAATGGGTAACACAAAGCTGCAGGTTTTAATTTTGCTTTGTCAATATGTT700.10548046351129393No Hit
TCTCGTGGGTCGTAAGCTGGTGAAAAAAAGAATAAACTTTGCCTCTCTGATA690.10397359974684689No Hit
GACGCGGTATGACAGAATTTGGTGCACAGAAATATAGAACACAAAACCAAAT690.10397359974684689No Hit
ACCCGATGGGTCAATCACATCTTGATTCAAACCTCACAAGGCAATATTCTGA690.10397359974684689No Hit
GTGTCTGGGAGGGATTCTTGCGCCTCGGCCTCCGAGTAGCTGGGATTACAGG690.10397359974684689No Hit
GGCTATGGGAACTCCCTGGATTCAAGTAGCACATTGGTAATAGGTATCAGAG680.10246673598239982No Hit
GTATATGGGGTGAAGGACAGCAAGGGTTGCCTCCTCCACTGCAAGAGGGTCT680.10246673598239982No Hit
GGACCTGGGAATATAGCCTCCATAAATGTTTTTAATGACAATATTCTGTTGA680.10246673598239982No Hit
ACAAAAGGGTCTGATACTGGGGAGATCAAAAATGGCGGTAGTTGGTGTGTCC680.10246673598239982No Hit
GCGCCCGGGTTGCTAATATATGAGGTAATGCATATGTTAACTAACTTAATGA680.10246673598239982No Hit
TACTTTGGGTGTATTGTCTCTGCTAGGAAAAATAAGTATAATAAGTTAAGAC670.10095987221795277No Hit
AAGCATGGGAATGAAGCCAGGCATGAAAAGACAAAGGAGTTTATACAGAGAA670.10095987221795277No Hit
GGCACAGGGATAACCATGCACACTACTATAACCACCCTAACCCTGACTTCCC670.10095987221795277No Hit
TGGATTGGGTAATTACATGCTATTAGCATTGAGAAATGTTGACAAATTAATT670.10095987221795277No Hit
ATCGCTGGGGGGGCAGCAGACCGTCCAAACCGACACGCGTGGTATCCTCGCG670.10095987221795277No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)