FastQCFastQC Report
Sat 31 Dec 2022
SRR5848121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65959
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGCCGTCT5360.8126260252581149No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGC5060.7671432253369518No Hit
GCGCGGGGGACTAATGACAGCTGGTGAGCTTTAAAAGCAAACAAACAAAAAA1480.22438181294440487No Hit
AAAACTGGGTGAAATTAATGAAATTAGTGCAGTGTCTCTCTCTCTTTTTATG1300.19709213299170697No Hit
TTGCCCGGGATAAGCAGTTATTTTGGGAAACAGAAGCATTTAAAATCCATTT1160.17586682636183082No Hit
GGTAATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1130.17131854636971452No Hit
GATATAGGGCTGGAGCAGGAAGATAAAATGAGCTTGAGCTCTGGCAAGCCCA1090.16525417304689277No Hit
AGAATGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1070.16222198638548188No Hit
GCAGACGGGCTACAGATGGGAGAAAATTTTTGCAAACTAGGCATCTGACACA1020.1546415197319547No Hit
TTCGCTGGGTGGATTCTGAAAGTTAAAAATTTAGTTGTTCTCCCCAATGTGT1000.1516093330705438No Hit
AACATAGGGCTGACTTGCTTTGAAGTCTGTTATTTTAATCACTCCAACATGA960.14554495974772205No Hit
TCTCAGGGGAGAACAAAAGCAATACTGAATTTCCCTTTCAACTCAAAGGCCA960.14554495974772205No Hit
AGACGCGGGATGAGCACTGAGCCATAAAAGGTTTTCAGCAAGTTGTAACTTA960.14554495974772205No Hit
TTAAAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC950.14402886641701665No Hit
GACTAGGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG950.14402886641701665No Hit
GCTAGTGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA940.1425127730863112No Hit
GTGTCTGGGAGGGATTCTTGCGCCTCGGCCTCCGAGTAGCTGGGATTACAGG920.13948058642490033No Hit
CCACATGGGTGTGAAGAGTCTGCTTAAACATTTAACAGAGATGGGCTCACTT910.13796449309419487No Hit
GTAATTGGGTGGCTAGTTGCATTTGTAAATAAGTATAATGTTGCTTTGATTT900.13644839976348944No Hit
CGATATGGGAGTGCTACAAAGGACAGTCGAAGCTAGGAAGTGCTCAGCAGAG900.13644839976348944No Hit
TTAGCTGGGGATTCTGGGTCATCAGAAGTTCATAAAACACTAAATTAAGGTT890.134932306432784No Hit
TAGCTAGGGCATCAAAAAATAGAAAGATCACAAGTTAACAAGCTAACAATGT880.13341621310207857No Hit
TGTCTCTATTAAAAAAGAAAAAAGAAGAAGAAAATTAGTAAGGAAATAGAAC870.13190011977137311No Hit
GAAATAGGGAGATAAATGAAACAAAGAGCTGGTTCTTTGAAAAGATAAACAA870.13190011977137311No Hit
ATGCAGGGGTTCTAGCCAGTGCAGTAAGGCAAGGAAAAACAATAAAAGGCTT860.13038402644066768No Hit
GACAATGGGAAGGGTAATGTAGCAGAAGACAGGATGGGTTTCTCTTGCCTGA860.13038402644066768No Hit
AGTCCAGGGTATTCTACCTATAAAATTGCCTGTCGTTAACAATTCAGTAACA850.12886793310996225No Hit
GGATTTGGGTCATGGCTGAATAACAGTATCGAAATGTTATCATAATTCTTTG840.12735183977925682No Hit
TCATAAGGGCTGCAGATGGGGGCCCGGGAGGCACACACGAGTCTGGGACTCA840.12735183977925682No Hit
TCTCTTGGGGGTCTTTTCCAGACATTTCATATAAATGGAAACCTTACAATAT840.12735183977925682No Hit
GTTACTGGGGCTTTCATTCTAACATTCAGACATAGCAATCCAAACCCTTGTT840.12735183977925682No Hit
TCTCACGGGTTGATGGAAGTTGGAAGAGAACCATGGGAGTTCACTGTATAGA840.12735183977925682No Hit
TCTTCCGGGATGAGAAGAGCTCTCGTTTCATGAAAGTTGGCTATGAGAGAGA830.12583574644855136No Hit
TACAACGGGTGATTACTGCAGAGCAGCGTTAATGGAGTGCTTACTTTACATG830.12583574644855136No Hit
GTAATGGGGTAGTACCAACAAGTACCATTCGTATTATGATTCACAATTTACA830.12583574644855136No Hit
CATTAGGGGAACTCAGTAGAAGGGCTTAAGAACAGATTTGGCCTAGCATGAA820.12431965311784593No Hit
ACACATGGGAATGTGAGCCTGAGAGAGAGTGGGAAAAGTATAGAGTGAGTAT820.12431965311784593No Hit
AAACATGGGTATCAGTATTAAGTAGACAGCATATTATTCAAGTGTTTAGACT820.12431965311784593No Hit
TTAACCGGGAGTTTTTGAGGAGGAGAGACAACTAAGTTCAATTAACACCACT820.12431965311784593No Hit
CCAATTGGGGATACAGGAAGGCACTGGCTTACTCGGGTGCTCTTACCTTCTA820.12431965311784593No Hit
ACATAGGGGGAATATCTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.12280355978714048No Hit
TGAAGCGGGGTGTACAAATGACATCAAATATAAAAATAATTACAGGATGTTC810.12280355978714048No Hit
GAAAAAGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT810.12280355978714048No Hit
TCATAGGGGTCTTCAACAACATTGCCTACCGCAGCTCCTCAGCCAACCGCCT800.12128746645643507No Hit
CGCGCGGGGATTTGGTGAAACATTATTCTGCGTGTTTCTGTGAATGTGTCTT790.11977137312572962No Hit
GCCATGGGTGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATT790.11977137312572962No Hit
TCACAGGGGCATGAGGACAGAATTACTTTGTTTGTTTAAATTATCTTGCTAG780.11825527979502418No Hit
TTCTGCAGGGGGAGACGCGCACGCTCACGCAGTTCCACTTCCTCAGCTGGCC780.11825527979502418No Hit
TACGGTGGGTTACTGATGCTTGGTGTATATTTTGATTAATTTAGAGACATTT780.11825527979502418No Hit
GAGCATGGGGAGTGTGCAGGCTTAGAGGGACTAGGCTGGGTGTGGAGCTGCA780.11825527979502418No Hit
CGTCTGGGGAGGCCTCTGAAGAGAAACTGCCTTTTCCCTCTTTCAGCCAAAA780.11825527979502418No Hit
TACAGTGGGAGACCATAATGAACTGCCAAAGAAGCTGAAACCTAAAATAAAA770.11673918646431874No Hit
TGTTACGGGAATTCCCTACCATATGTGCTTGTTACATTTTCAATATGAAAAG770.11673918646431874No Hit
GTAACTGGGGGTTATGTTCCTCATCCTGGCTCAGTTACTGAGTTTCCTGTGT770.11673918646431874No Hit
AGCAGTGGGGAAAAACTGCAGTATCTAGTCACTTTTCTGAATGTCTGGGCTA770.11673918646431874No Hit
TGGATTGGGTAATTACATGCTATTAGCATTGAGAAATGTTGACAAATTAATT770.11673918646431874No Hit
GTTAGGGTGAATGGAGTAGTAACATGTATAGATAGGTGACTTTTTTAGGATA770.11673918646431874No Hit
CGCGCGGGGTATCCCACTGATTTTTTTAGTATTTCGTTATTTTCTGACAAGA770.11673918646431874No Hit
GTTCCCGGGAGATGAAAAGAACCCTTTAAAAGAGTGCCTTCCACATAGCCAT770.11673918646431874No Hit
ATACCGGGGATCAAATATTTAGAAAAAAAGTACAACTTATATATACTTTTTC760.1152230931336133No Hit
CATCTAGGGATAAAACACTGAGTGCTGTTTTCACAGTATCAATTAGAGAATT760.1152230931336133No Hit
GGATGCGGGCAAGGGTGACGTGTTTGTGGCCCCCCGGACAGACCACCCAGCC760.1152230931336133No Hit
GATGAGGGGATGGACGCTGATAGACGTCCTGCTCATTGGAGTTGGCTATGTT760.1152230931336133No Hit
ATGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAA760.1152230931336133No Hit
ATTCTTGGGTGAGACCCTGTCTCAGTAATTAAAAAAATATAAAATTTAAGGG760.1152230931336133No Hit
GAAACAGGGAGAGCAATAAAAGCTCAAATTCCAAGTGTAACTTCTGTATTTG760.1152230931336133No Hit
GAACTGGGGTGATGCACACATTTTCAATGTGCTACTGTATGACATATTTATA760.1152230931336133No Hit
ATGCGAAGGGTCATAGATAGTCATTTTGCCTCCTGGACAGTTGGCTGACGAA760.1152230931336133No Hit
ACTTTAGGGTGTGCTAGGAAGTGTTAAGTGCTTTATAGATATTATTTCCCTC750.11370699980290788No Hit
GCCACAGGGAGTATAAGTGCATATTTCTTGTCCTTTTTCTCCTAATTAATTT750.11370699980290788No Hit
TGATGAGGGAGCGAGGCGGCCGCAGGTACCGCAAGATGAAGGAGCGGCTGGG750.11370699980290788No Hit
ATAACAGGGTGATATCTGAGCATGATATTTGGAGAGGCCATATATCCAAACT750.11370699980290788No Hit
CTTACGGGGGCTCTATCTTCATGTACTCATCCTGAATCCTTATTTTTCTAAA750.11370699980290788No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCC740.11219090647220244No Hit
AGACGGGAATAAGCTGCAATTTTGTACAGATACCAACTTCTGAAAAGCTGGT740.11219090647220244No Hit
CGCTGCGGGTCACCTTGTAACCTGAGTTACAAATAGACCTTTTCTTTCAGAA740.11219090647220244No Hit
CTTAATGGGATGCATTCTCAGATTATGCCAACTTCAAGTAAGCGGCAACAAG740.11219090647220244No Hit
AAAGGACGGGGATCTGACATGGGGTAATGAGAGAATGAATGAGCCTTTGGAG740.11219090647220244No Hit
GACGTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC740.11219090647220244No Hit
TCCTGTGGGTTCACCTTTTTGGTTACATTCATTTCTAAGTATAGTTACTGCT730.11067481314149699No Hit
CATTATGGGTATCTTATAAATGATATATTTTTATATCCCATGCAGTAAGAAA730.11067481314149699No Hit
CTCACCGGGGAGAAGTCAAGCTTGAAGTAGAAAATGTCATGATGAGAAAAAT730.11067481314149699No Hit
TCTTAAGGGTCTGCTTTCCTAAAGCTATGCAGGGCTCCCCAAGCAATTGGAA730.11067481314149699No Hit
GCTCAGGGGATCACGAATTACAGATGACATGATCCTTTATGTAGAAAACCCC730.11067481314149699No Hit
TGCAGGGGTGCTGTTGCATACTTATTTATACTAGATGCTTCATATGCTTGAA730.11067481314149699No Hit
CCGCTTGGGTGACTGGCATTTGAGATGCTGCTGGCATTTTGAATTCAACATC720.10915871981079155No Hit
TTTTAAGGGTGTATTTCTTGTATATTGTGATTTGCATTTTTAGTCTCTTAAA720.10915871981079155No Hit
TCTAATGGGAGGCTTTGAGAGTGTTTGAAGTAACTAATAGAAAAAGAAATTG720.10915871981079155No Hit
GCTACGGGGAGAAGTGAGGCATGTCTTACATGGTGGCAGGTGAGAAAGAGGA720.10915871981079155No Hit
GCTATAGGGATGGGAGTTCTTTCATGGCCTCTGCTTGGGGTGGGGGAGTCAG720.10915871981079155No Hit
CGGTATGGGTTACTGAATTTTGAACTTAAAAAATTCTAAGTAGTAGGATTTT710.1076426264800861No Hit
GGACCTGGGAATATAGCCTCCATAAATGTTTTTAATGACAATATTCTGTTGA710.1076426264800861No Hit
CTAAGTGGGTAATTTGTTTTTAAAACAACTAGATCAGCTTGTTGTTTATTAG710.1076426264800861No Hit
ATGAGCGGGTGATGTAAAATCCAGGCTATCAGAGCTGGGTCACAATTTAGTG700.10612653314938068No Hit
ACTACTGGGACCCTTGAACAACACGGGTTTGAACTGTGTGGATCCACTTATA700.10612653314938068No Hit
TCAAGCGGGTGCTATCTGCAGATAGAGTGTGAGAATTGACTTAAGAGGATGC700.10612653314938068No Hit
GGCACAGGGATATGATTTAAGTGCATGAATAAGCATTTTCCCACACAATGAA700.10612653314938068No Hit
GATCTAGGGTCCGATTTAACTGTGGGGCATTCAAATGTGTTCATGTATTGTA700.10612653314938068No Hit
TCTGCGGGGAACTCAATTACAAGAAGATCTCTGAAAAAACTTACAATTACAT700.10612653314938068No Hit
ACAAGAGGGTGCACTTGATTTTTTGAAAGTTAAGGCTAGTCACTTTCACTTT700.10612653314938068No Hit
ATGACTGGGTGGACTTGCACCTCATGTTTAATGTGAATATTTATTTCCATGT690.10461043981867524No Hit
TGAACAGGGATTCTTCAGATTGATATCTTTAAAGTGCCACCATTTCTCTTTT690.10461043981867524No Hit
GCGCATGGGTGTTCCTCCATTTTCAGCAACACAGCTAAAACCTTAAACCATC690.10461043981867524No Hit
TTGACTGGGTACTGCGTATTGAAGAAAAAAATCTGACACCACCGAGCCAAAG690.10461043981867524No Hit
GTCTCGGGGTAAAAACAAGTTAATCATCATTCATATCCCTTCTCTTGCATAA690.10461043981867524No Hit
GACGCGGTATGACAGAATTTGGTGCACAGAAATATAGAACACAAAACCAAAT680.10309434648796979No Hit
GGCTATGGGAACTCCCTGGATTCAAGTAGCACATTGGTAATAGGTATCAGAG680.10309434648796979No Hit
ACAAAAGGGTCTGATACTGGGGAGATCAAAAATGGCGGTAGTTGGTGTGTCC680.10309434648796979No Hit
TTTGGCCGGGGTGGCATCTAGAACCTACTCTTAGATTAATTTCTTGGACTGG680.10309434648796979No Hit
CCTGCTGGGTGGTATTCCACAGGAAACAATTTCTGGAAACAAGAATTATTTG680.10309434648796979No Hit
CGAAATGGGTGTGATCCTTCTTCTGAAAATTCAGTGGCTGGCCTCCAGACCT680.10309434648796979No Hit
ACTGCATGGGAATAAAGGAATGGAAAAAGATATTTCATGCTAAAAGAAACCA680.10309434648796979No Hit
CCAGGGGATAGAACTAAAACATGAAAAGTAACTACTACAAAAGCACTGTAGA670.10157825315726436No Hit
GGCCCCCCATGGACCTGCAGCCGCAATCCTCAGATGAGCAAGTGCCGCTGCA670.10157825315726436No Hit
ATGCATGGGAGAGGGCAGAGTTCTTTCTTGGGTTTGAACTGGCTTTTTTGGG670.10157825315726436No Hit
GTCCTTGGGTATATAACATAAAACTCACCAGTATCAAGTGCACGATTTGAAG670.10157825315726436No Hit
GCTTCAGGGGAGATACAGAAACCGACAGGGGCCAGGCGCCCGGTGGCTCCGA670.10157825315726436No Hit
AAAGCAGGGTCTAGCTCTCAAGCTTTGGGTGAATATTCCTGTGAAATATATC670.10157825315726436No Hit
ACACTAGGGATTTAATGTAAAACCAAAGTGACCTGGAAGCAAGATTATCTTA670.10157825315726436No Hit
CGTCTCGGGGCCTCACTTAAAAGCTAAACCCAGCATTTGTTACAGGCCTTTT670.10157825315726436No Hit
GTGCAGGGGACAGGCATCTATGGGATGTGGAATCTGTATGTCTTTGCTCTGA670.10157825315726436No Hit
CATACAGGGTATCATCTATGCCGGTGAGAAGGCCCAGTTTGCACAGCCGGAG670.10157825315726436No Hit
ATCGCTGGGGGGGCAGCAGACCGTCCAAACCGACACGCGTGGTATCCTCGCG670.10157825315726436No Hit
GTTGTGGGACGGCCTACCTGGAATTTAAAATTCAGTAGACAAATTAATTGGT660.10006215982655892No Hit
TCCAGTGGGTATCAGGACTCCATAGTTCTCCAACAGAGAAATCTTGAAAAGG660.10006215982655892No Hit
ATTTGAGGGAGACCCCACTGGACTGTTGGGTGGAGAAGAGAAGGGGAGCCTC660.10006215982655892No Hit
ACCAGCGGGCTGAGTCCCTTATGATACAGCCTGAATGCATCATGACAGATCC660.10006215982655892No Hit
GTTTCTGGGACCTATCACACTTTGTTCTCCGCAATGAATTCATCTTCTGTCT660.10006215982655892No Hit
AGTGCCGGGTCTATCTGCCATGATGTGATATGATTTATAATGATTTAAACAG660.10006215982655892No Hit
GAAATCGGGTACTTGGAGAAACTAGGTGAAGATGCAACCAGAAAGGAGAATA660.10006215982655892No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)