FastQCFastQC Report
Sat 31 Dec 2022
SRR5848117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46177
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGCCGTCT4440.9615176386512766No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGC4340.9398618359789507No Hit
CGAATGGGGATGTAGTGACACCCATAGGCTCAAAGTAAAGTAATAAAGAAAA1120.24254498993005175No Hit
TTCCACCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1110.24037940966281915No Hit
ATTCGAGGGAGTGCAGCTCCACATTAACTCTACAGACCAAACCATTTGTATC940.20356454511986488No Hit
TCCTGGAGACCCGCAAGTCCTGGAGAAAGCCTGAGAAGACAGACTGAGCAGG890.19273664378370184No Hit
GAAATGGGATACTGCCGAGTCTGGGGGCTTGTTTTGGTTTGGGGGCAGCCAT870.18840548324923662No Hit
ACCACAGGGGGTCAAAATACAGTCTTCAGCTAATGCTTTCTTCCAGCTGGTT850.18407432271477142No Hit
AGCTCAGGGACTTATGGTTTTATAATATATTTAATTGGATTATAATATTCCT820.17757758191307363No Hit
GCGAAAGGGGAACATAGAACCCAGAAATAAATCCACATATTTATAGCCAACT790.17108084111137578No Hit
TACCACGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA780.1689152608441432No Hit
AATACCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA780.1689152608441432No Hit
GCTTAGGGGATCTTACCATGTACATTATAGATGACTTACAGAATATTTTGGT750.16241852004244536No Hit
TAAAAGGGGGGGGCAGCTTCCGGCGGGATGTTGAACTGGTCATTAATGTGTC740.16025293977521277No Hit
CAATCTGGGCAGCTCGGAGGAAGCTTGGAGGAAACTCAGATTACTTGGTATC730.15808735950798017No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCC730.15808735950798017No Hit
AGCGCTGGGTACTACAACCCTTCGCTGACGCCATAAAACTCTTCACCAAAGA730.15808735950798017No Hit
CAAAGAGGGGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGA720.15592177924074757No Hit
TCAATAGGGAACATAAAGCAAGTGAGCGACCTTATCTCTGTGCTTCGTGAAG680.14725945817181713No Hit
AGTACAGGGGCAACATGGAGAGAATCATGAAAGCACAAGCGTACCAAACGGG680.14725945817181713No Hit
AAGCTTGGGCCCCCTGAACATCCCCCTGCTTGCTGACGTGACCAGACGCTTG680.14725945817181713No Hit
TTTGCCGGGTCTAGAGGTGAAACAGGCAAGTGAAATGGACACCTCTGCTGTG680.14725945817181713No Hit
TCGCAGGGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTT680.14725945817181713No Hit
ACAATGGGAGAGTTGTGAAATTCAGCAAAGAGATTTCTACAGGAGCTAAGTC670.14509387790458453No Hit
GCAATCAGGGGAAAAGATGGAGTCAGCAGATCAGGCCGTTGCTGAGGTTGGA670.14509387790458453No Hit
ACTTAGGGGTGGTTCTTCTGTTTGCATATATTAATCTGTAATTAGCATGAAT650.14076271737011933No Hit
GATTTAGGGCCATCGCTGGGTCAATAGTACTTGCCGCAGTACTCTTAAAACT650.14076271737011933No Hit
GTAGTTGGGCGGGCTACTACAACCCTTCGCTGACGCCATAAAACTCTTCACC650.14076271737011933No Hit
CATTCGGGGGGGTCTGCGCCGGAAGTGCATGAGCTGCCGATGTGGTGCTTAG650.14076271737011933No Hit
GTCTCAGGGAGGCAGATTACATTAGACTTCTTCCATCACAAAGATGGCACTA640.1385971371028867No Hit
TGTAGTGGGCAGTTCCAGAGGGTTAGCCCCAGCACCCCACTCTCCTGCCACT640.1385971371028867No Hit
TGCTAAGGGGTTTCCAAAGTTCGTGCCTGCGAACCCAAACCTGTGAGTCTCT640.1385971371028867No Hit
TGTTGCGGGTAGACGCGGGACTTCAAGTAGCCATCAACAACAACAGAGGATT630.1364315568356541No Hit
CAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCC630.1364315568356541No Hit
GAAAAGGGAATCCATGTCGTCACAAGGATATCTGGCAAAGGAAACCAAGCTG600.1299348160339563No Hit
TATCTCGGGGCCTGCGGGGCGGGGGCGGGGTGGCGCCCAGGACGACTCCCCG600.1299348160339563No Hit
GTAGAGGGGCAATTTCATCACAACAATTATCAATATAAAACCCCCTGCCATA600.1299348160339563No Hit
ACTAGCGGGAATGTACTGATCTATATGGTTTTATAACTCACATCTATATGGT600.1299348160339563No Hit
TGGCTCGCAGGGTCGAGATAAGACCATCATCATGTGGAAACTGACCAGGGAT600.1299348160339563No Hit
TGTACGGGGCAATGGGCTTTGGACCATGTGGAGGTCGTCCTAGTAAGCACTC590.1277692357667237No Hit
GTACTGGGGATAGTCAGGGCATTTGAGAGGAATGATTTCTTTGACGCTGGCA590.1277692357667237No Hit
AACACTGGGGGACTTTGAAGGACCAAAGGTTTTAAAAGTCAATTGGTATCAC590.1277692357667237No Hit
AATGTAGGGACAATGTTATAGAAAAATGCCTTGAATTCTAGTGTTGTTGTCT590.1277692357667237No Hit
GCTCATGGGTATTCAGAAAGGTTAAGCAGTGCAGGGCGTCATGTGCTTACAG580.12560365549949107No Hit
TCGAAAGGGAAATAAAGGGTGCCAGACAGTCTGCTATCTGCATTTATCTCTA580.12560365549949107No Hit
TAAGCGGGGGGGCGTTTTCATGCTGTACAGTGACCTAAAGTTGGTAAGATGT570.12343807523225848No Hit
CCAACTGGGGTCACAGAATCTCCATTTCTGCTCAGATTCCCCCCATCTCCAT570.12343807523225848No Hit
GTGTGTGTCTTTGGAGCTATATATTGTTTTTGGAAGAGCAAAGTTTTGTTGT570.12343807523225848No Hit
TGTCTCGGGTGTGTGTGAAATTGGAAGCAAGGTATGTGCAAGAGGTGAAATA570.12343807523225848No Hit
TGTGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA570.12343807523225848No Hit
GCATGTGGGAACACTCTGAACCCCTATTACAACGAAGCTTTCAGCTTCGAGG560.12127249496502587No Hit
GCTCTTGGGCACCACAGATCAAGAAATAGAACATTACTAGACTGGGGTATAT550.11910691469779328No Hit
GCACGAGGGTCCAACTGACCTGGGACCAGGTGTCGCGCAGAGATGTGCCTCA550.11910691469779328No Hit
ATCGTGGGGCCGATCTCGGTGAGGGATTCACCAAAGTATTGTTAATTAGCAG550.11910691469779328No Hit
AAATGAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC550.11910691469779328No Hit
TTTAAAGGGGGAGGCGAAGAACGCAAAGCTGAGAACATGGACGTTAATATCG550.11910691469779328No Hit
CAGCCTGGGCGACAGAGCAAGACTCTGTCTCAAGAAAAACAAACAAACAAAC550.11910691469779328No Hit
ACACAAGGGGCTCTTCCGATCTCTATGTGGGACCTCACCTTTAGTATTATGT540.11694133443056066No Hit
TGCACAGGGGCGTTGTCGATGGTGGGGGTTATTTGCGGGCTGATTGAACACC540.11694133443056066No Hit
GACAGTGGGATTTCAAGAAGGACAAAGGTATGTGTGCATATTTAAATAACGA540.11694133443056066No Hit
AATGGGGGTGGAGGGAAGATGGCGGACGAGGAGAAGCTGCCGCCCGGCTGGG540.11694133443056066No Hit
ACGCTAGGGGTGGAATTTTAAGTACACTTGACTCTGAATAATGTGGTAGTTG540.11694133443056066No Hit
GCGACGGGGAGGCTAAGAGTTAAGAAGTTGAACAGAACTCAGTAGTCAAAGC540.11694133443056066No Hit
AACGCAGGGATCCTTGGGGTCCTGAGGTCTTATGCAGTGGTTGGGGTCCCAC530.11477575416332807No Hit
ACTGATGGGATAGAAGGGCTGTAATAGTAGTATCAAGCTCACAGGATTGTTC530.11477575416332807No Hit
ACGTAGGGATTTGGGTGAGAACTGAGAGGTGGGAGATGATTGGAGGCCATGG530.11477575416332807No Hit
ATCACTGGGCTGTGCATTGTAAGTTATAGGAGCAGGACATATTTTTTGTACA530.11477575416332807No Hit
CACATTCGGGGAAATTTAAAAGATTTTTAATAGCAGTCAGGTATTTTCAGAG530.11477575416332807No Hit
CCAACGGGGATGTTAAAATTATTATTTTTAAGTCAAAAATATGGTATTTACT530.11477575416332807No Hit
TCTGTCGGGAATAAATTTCCATTCCAACTCTCAAGTCTGTAATTTGACTCAG530.11477575416332807No Hit
GGGCTAGGGAAAGAGAAACCAAATGAGCTGATTACTGACTATAAGTTCTCAG530.11477575416332807No Hit
ACCATCACCCTCTACATCACCGCCCCGACCTTAGCTCTCACCATCGCTCTTC530.11477575416332807No Hit
CACAGCTAGAAATGCAATGACTATGTCTGCTATCACCACTGTTACTACTGAG530.11477575416332807No Hit
TCTTGTGGGAGAAGTTGGGTATACCAACCACCACCACCACCGCCCCCTATTC520.11261017389609546No Hit
ATTGGAGGGTGCTTTCGAAGTAACTGTGGTCTTAGGTTCGGCTGAGTAAAGA520.11261017389609546No Hit
CAACTAGGGACCAATCTGGGGGCTTGTTTTTCTTTTCTTCTTTCAAATAAAT520.11261017389609546No Hit
GTTCGCGGGGGATGAAAAAAGGTATACCATGTTAATACAAACCGAGAGAAAG520.11261017389609546No Hit
GGTATAGGGAGACTGGCGCGCGCGGACGGTCATGGGACTTCAGCATGGCGGT520.11261017389609546No Hit
TGCTGAGGGGGTAACTGTCAGTGATGAAATAAGACTAGTATTCGTTCAAGGT510.11044459362886286No Hit
CCGACTGGGGTAGAGAAGTTGTAGTCGAAGGGAATGTTGGGACTCCTCACTG510.11044459362886286No Hit
AGTTGGGGGGTATTTAGCATTGTAGAAACTGTGAAGAATCTGCACATACTCC510.11044459362886286No Hit
TCAACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA510.11044459362886286No Hit
GTAGCAGGGAAGATGAGAATGTTGTATTAGGTGGACTCTTCTAATCCTGAAT510.11044459362886286No Hit
AATCCAGGGGGAAGAAAAGTCATGGATATTTTAAAAAGAGAGTGCAAGTAGC510.11044459362886286No Hit
GGTTCCGGGAAACATCTTTGGAATTGTGAGTGAAGGATATAGTGGCAGGCTT510.11044459362886286No Hit
TCAAAAGGGCATCACTTTCAGATTTCTGGAGGGAGAGGGGTTGGAAGTAATC510.11044459362886286No Hit
CAAATCGGGGAGTTATAGAGCAAAGGGATGGGTCCTTGAGGCCTGCCAGTGT510.11044459362886286No Hit
TGCAAGGGGTTCTTTTCTAAATCATAATTTTAGACCTTTTGCTATGTTCGCA510.11044459362886286No Hit
GCCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA500.10827901336163025No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGT500.10827901336163025No Hit
GTGTTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC500.10827901336163025No Hit
CTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCA500.10827901336163025No Hit
CGTACAGGGGGCCGCCGCCGGCGGCTAGCGGGCGTCCGCGCCATGGAGCGCT500.10827901336163025No Hit
TGAATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA500.10827901336163025No Hit
TAGCCTGGGGATAGAGATTAATACAACTACTTAAAAAATATAGTCAATAGGT500.10827901336163025No Hit
GCTAACGGCGAGTGGACAAAGCTGAGCATGTTATTTTAAGACATATGTCCTT500.10827901336163025No Hit
TTCTCAGGGATGCCTCATGCCCCCAGCTTCGACATTACCAAGTTCCGTTCCA500.10827901336163025No Hit
ACATATGGGACAGTGGTCTTTAAAATGGAGCAATTCTTGTTTTTTTTCAAGT500.10827901336163025No Hit
ACACAGGGCATTGTCTATGAATTGGACAAGAACTTGAAGCCTATCAAGCCCA490.10611343309439765No Hit
TATAGCGGGAGATGTAAATGAATTAACTGCTTCTATCAAAAAGCAGATTGTC490.10611343309439765No Hit
TAACACGGGGCTCTGCACCAAGCGGACCTAATAGACATCTACAGAACTCTCC490.10611343309439765No Hit
CACGCAGGGAAGCTGAATCCTCACTAATTCACTTTTGAAAAGTTCTTAATGA480.10394785282716504No Hit
ACGTAGGGGCTTCACCTCTCACATACTAAAGCATTCATTAAAAATACAACTA480.10394785282716504No Hit
AAAGGTGGGGTGGAGGGCGTGTCCTGCGGCGCGATGGCCGTAGTGTTGCCGG480.10394785282716504No Hit
ACTTGGGGGAATATCATGGCTATGGAACAGTAGAGAGAGACAGGCCTGATGC480.10394785282716504No Hit
TCGCTGGGGGTTGGTGGCTGACTTTTCAGAAGGAATTATTCACCTCACATGA480.10394785282716504No Hit
TGGGGTGGCTCCAGGAAGCCTCATCCAGTGATATATTTCTCATAGCATTCAT480.10394785282716504No Hit
TCTCGGGGGCAGCCAAAAACCCAGAACGCTGGACAAACATTCCTTTGCTGGT480.10394785282716504No Hit
TTTGCAGGGAACCATGTGCATGGACAGCCATATACAGGCCCAGCAGCACATC470.10178227255993244No Hit
AGCAATGGGCTTGGTCACACTGATTAAAACTAAAGATTTGTAATCTTTTTTT470.10178227255993244No Hit
GCAGTGGGCAACACTCACATCCCACTGGCTGCTGTTCTGAAACCATCTGATT470.10178227255993244No Hit
ATCTACGGGGGTGGCCGCAAGCCCTTCCCTGGGGAGTACCTGATGGCTGCCC470.10178227255993244No Hit
AAACAAGGGGATTCCTATGATTGGGAAGCTGGAACCACGAGAAGATGCTATC470.10178227255993244No Hit
TTCGATGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG470.10178227255993244No Hit
TTTGCAGGGATTGACACAAGTTATCAAAATCTCTGGAATATAGTAAAAGCAG470.10178227255993244No Hit
AACGCAGGGATGTGCATTTGGTATCATGGGCCGTGGAACAGAATATATGTTG470.10178227255993244No Hit
TCACAGGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCAA470.10178227255993244No Hit
CTGATGAAGGGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCA470.10178227255993244No Hit
GCGGTTGGGAACTTGGAAATTTGTACTGAAATCCACAATAATCAGTTTTTGC470.10178227255993244No Hit
GACATAGGGTCTGACGCAGGGTTGTGGTATATGCAATGTTTTAAGGGAAAGA470.10178227255993244No Hit
AGCCATGGGGGTGGAGAGAGCGCGGAGACAAGCGCAGAGCGCAGCGCACGGC470.10178227255993244No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCA470.10178227255993244No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)