FastQCFastQC Report
Sat 31 Dec 2022
SRR5848116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46693
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGCCGTCT4630.9915833208403829No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGC4620.989441672199259No Hit
TTCCACCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1110.23772299916475703No Hit
ATTCGAGGGAGTGCAGCTCCACATTAACTCTACAGACCAAACCATTTGTATC1040.22273145867688945No Hit
CGAATGGGGATGTAGTGACACCCATAGGCTCAAAGTAAAGTAATAAAGAAAA990.21202321547126976No Hit
AGTTGGGGGGTATTTAGCATTGTAGAAACTGTGAAGAATCTGCACATACTCC820.17561518857216285No Hit
ACCACAGGGGGTCAAAATACAGTCTTCAGCTAATGCTTTCTTCCAGCTGGTT770.16490694536654316No Hit
CAATCTGGGCAGCTCGGAGGAAGCTTGGAGGAAACTCAGATTACTTGGTATC760.16276529672541923No Hit
GTACTGGGGATAGTCAGGGCATTTGAGAGGAATGATTTCTTTGACGCTGGCA750.1606236480842953No Hit
GTAGTTGGGCGGGCTACTACAACCCTTCGCTGACGCCATAAAACTCTTCACC750.1606236480842953No Hit
TGTTGCGGGTAGACGCGGGACTTCAAGTAGCCATCAACAACAACAGAGGATT750.1606236480842953No Hit
TGTGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA750.1606236480842953No Hit
TCAAAAGGGCATCACTTTCAGATTTCTGGAGGGAGAGGGGTTGGAAGTAATC740.15848199944317135No Hit
AATACCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA730.15634035080204742No Hit
AGTACAGGGGCAACATGGAGAGAATCATGAAAGCACAAGCGTACCAAACGGG720.1541987021609235No Hit
TGTCTCGGGTGTGTGTGAAATTGGAAGCAAGGTATGTGCAAGAGGTGAAATA720.1541987021609235No Hit
TACCACGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA690.14777375623755165No Hit
AGCTCAGGGACTTATGGTTTTATAATATATTTAATTGGATTATAATATTCCT680.14563210759642775No Hit
GTAGCAGGGAAGATGAGAATGTTGTATTAGGTGGACTCTTCTAATCCTGAAT680.14563210759642775No Hit
TCAATAGGGAACATAAAGCAAGTGAGCGACCTTATCTCTGTGCTTCGTGAAG670.1434904589553038No Hit
AAGCTTGGGCCCCCTGAACATCCCCCTGCTTGCTGACGTGACCAGACGCTTG670.1434904589553038No Hit
TCTTGTGGGAGAAGTTGGGTATACCAACCACCACCACCACCGCCCCCTATTC670.1434904589553038No Hit
TCTGTCGGGAATAAATTTCCATTCCAACTCTCAAGTCTGTAATTTGACTCAG670.1434904589553038No Hit
TCGCAGGGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTT670.1434904589553038No Hit
AGCGCTGGGTACTACAACCCTTCGCTGACGCCATAAAACTCTTCACCAAAGA670.1434904589553038No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCC660.14134881031417987No Hit
CGCTTTGGGGCATAACTGAAAGTAGCTAAGGCACCCCAGCCGGAGGAAGTGA660.14134881031417987No Hit
AATGGGGGTGGAGGGAAGATGGCGGACGAGGAGAAGCTGCCGCCCGGCTGGG660.14134881031417987No Hit
GCTTAGGGGATCTTACCATGTACATTATAGATGACTTACAGAATATTTTGGT660.14134881031417987No Hit
CAAAGAGGGGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGA660.14134881031417987No Hit
TAAAAGGGGGGGGCAGCTTCCGGCGGGATGTTGAACTGGTCATTAATGTGTC650.1392071616730559No Hit
ACAATGGGAGAGTTGTGAAATTCAGCAAAGAGATTTCTACAGGAGCTAAGTC650.1392071616730559No Hit
GCGAAAGGGGAACATAGAACCCAGAAATAAATCCACATATTTATAGCCAACT650.1392071616730559No Hit
CAGCCTGGGCGACAGAGCAAGACTCTGTCTCAAGAAAAACAAACAAACAAAC650.1392071616730559No Hit
TAAGCGGGGGGGCGTTTTCATGCTGTACAGTGACCTAAAGTTGGTAAGATGT640.13706551303193198No Hit
CACATTCGGGGAAATTTAAAAGATTTTTAATAGCAGTCAGGTATTTTCAGAG640.13706551303193198No Hit
GTTCGCGGGGGATGAAAAAAGGTATACCATGTTAATACAAACCGAGAGAAAG640.13706551303193198No Hit
AGTTTAGGGCAGCCCAGATGGTCATCCATATGAGAAAATCCACACTGCAGAG640.13706551303193198No Hit
TGGCTCGCAGGGTCGAGATAAGACCATCATCATGTGGAAACTGACCAGGGAT620.1327822157496841No Hit
AACGCAGGGATGTGCATTTGGTATCATGGGCCGTGGAACAGAATATATGTTG610.13064056710856017No Hit
ACGTAGGGATTTGGGTGAGAACTGAGAGGTGGGAGATGATTGGAGGCCATGG600.12849891846743622No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGT600.12849891846743622No Hit
ACTATAGGGTATGGTATCTCTCTAGATTTCAAGGAAACACACTGGATACTGC600.12849891846743622No Hit
ACGGCTGGGGCTTACTGGGGGCTGAAGGTTCTGGACATGAACAAGGGTCAGG600.12849891846743622No Hit
ACCATCACCCTCTACATCACCGCCCCGACCTTAGCTCTCACCATCGCTCTTC590.1263572698263123No Hit
GCTAACGGCGAGTGGACAAAGCTGAGCATGTTATTTTAAGACATATGTCCTT590.1263572698263123No Hit
GGTACTGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA580.12421562118518836No Hit
GTCTCAGGGAGGCAGATTACATTAGACTTCTTCCATCACAAAGATGGCACTA580.12421562118518836No Hit
TGAATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA580.12421562118518836No Hit
AGCAATGGGAGAGTCCAGCACCTGAGCCCTCCACCCTCCCCATTCTGGTTGG580.12421562118518836No Hit
TCCTGGAGACCCGCAAGTCCTGGAGAAAGCCTGAGAAGACAGACTGAGCAGG580.12421562118518836No Hit
TATAGCGGGAGATGTAAATGAATTAACTGCTTCTATCAAAAAGCAGATTGTC580.12421562118518836No Hit
CAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCC580.12421562118518836No Hit
ACTTGTGGGTACCGTGGGGCAGGAGTGCAGGGCTCCACAGGCGAGGGTTTCC580.12421562118518836No Hit
TCGAAAGGGAAATAAAGGGTGCCAGACAGTCTGCTATCTGCATTTATCTCTA580.12421562118518836No Hit
GAAATGGGATACTGCCGAGTCTGGGGGCTTGTTTTGGTTTGGGGGCAGCCAT580.12421562118518836No Hit
GCCAAAGGGCAAATACCAATGAAATGTAACTTTTAAATATTCCTCACTGTGT570.12207397254406442No Hit
TTCGATGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG570.12207397254406442No Hit
TTTGCAGGGATTGACACAAGTTATCAAAATCTCTGGAATATAGTAAAAGCAG570.12207397254406442No Hit
TTAGGTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC570.12207397254406442No Hit
CCAACGGGGATGTTAAAATTATTATTTTTAAGTCAAAAATATGGTATTTACT570.12207397254406442No Hit
ACTAGCGGGAATGTACTGATCTATATGGTTTTATAACTCACATCTATATGGT570.12207397254406442No Hit
ACACAGGGCATTGTCTATGAATTGGACAAGAACTTGAAGCCTATCAAGCCCA560.11993232390294048No Hit
AGAGCAGGGATGAGACAGGTTGGACAGTTTTAAAATCTCTTCTAACAAAGAA560.11993232390294048No Hit
AACACTGGGGGACTTTGAAGGACCAAAGGTTTTAAAAGTCAATTGGTATCAC560.11993232390294048No Hit
AACGCAGGGGCTGCAAATGTTGCTTAAAGAGCCTGTCACTATCTGTGGTTTT560.11993232390294048No Hit
TGCTTGGGGATCCAAGAAGACATTTCGAGCCTTGCCAAAGTGTGGTCTGAGG560.11993232390294048No Hit
TTTGCCGGGTCTAGAGGTGAAACAGGCAAGTGAAATGGACACCTCTGCTGTG560.11993232390294048No Hit
AGCCATGGGGGTGGAGAGAGCGCGGAGACAAGCGCAGAGCGCAGCGCACGGC560.11993232390294048No Hit
CTCGAGGGGAAATTCTGACTTTATATTTCTTTTCTCTCTCTAGGTCTGATAG560.11993232390294048No Hit
GTTCCTGGGGGTGGAGAGAGCGCGGAGACAAGCGCAGAGCGCAGCGCACGGC550.11779067526181655No Hit
ATCACTGGGCTGTGCATTGTAAGTTATAGGAGCAGGACATATTTTTTGTACA550.11779067526181655No Hit
TGTAGTGGGCAGTTCCAGAGGGTTAGCCCCAGCACCCCACTCTCCTGCCACT550.11779067526181655No Hit
CTGATGAAGGGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCA550.11779067526181655No Hit
GTAGAGGGGCAATTTCATCACAACAATTATCAATATAAAACCCCCTGCCATA550.11779067526181655No Hit
TACATTGGGAACTTGAAAAATACTGGTGGCGTCGATGGTGAGTGATTTTTAT550.11779067526181655No Hit
CAAATCGGGGAGTTATAGAGCAAAGGGATGGGTCCTTGAGGCCTGCCAGTGT550.11779067526181655No Hit
TTCTCAGGGATGCCTCATGCCCCCAGCTTCGACATTACCAAGTTCCGTTCCA550.11779067526181655No Hit
TGGGGTGGCTCCAGGAAGCCTCATCCAGTGATATATTTCTCATAGCATTCAT550.11779067526181655No Hit
ACATATGGGACAGTGGTCTTTAAAATGGAGCAATTCTTGTTTTTTTTCAAGT550.11779067526181655No Hit
GAAAAGGGAATCCATGTCGTCACAAGGATATCTGGCAAAGGAAACCAAGCTG540.11564902662069261No Hit
TCAACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA540.11564902662069261No Hit
GTTGTGGGATACTGTGAATTACTGAAATGTTGAACAAACCTGTGAAAGACAT540.11564902662069261No Hit
AGCTTTGGGACCTAATGAAAGGATTTGGTAATAAAGGTTATAATCACGATTT540.11564902662069261No Hit
CTTACTGGGACGGTGAACTCCAATGGGACCAGCGACCGCACAACCTCCCCTG530.11350737797956867No Hit
GCCAGAGGGGATTTGCGTCTTCTCAGTGTCTCCAGTAAAACCCTGAAAGCTG530.11350737797956867No Hit
TCGCTGGGGGTTGGTGGCTGACTTTTCAGAAGGAATTATTCACCTCACATGA530.11350737797956867No Hit
GCGACGGGGAGGCTAAGAGTTAAGAAGTTGAACAGAACTCAGTAGTCAAAGC530.11350737797956867No Hit
CATTCGGGGGGGTCTGCGCCGGAAGTGCATGAGCTGCCGATGTGGTGCTTAG530.11350737797956867No Hit
ACAGCCGGGATTGTACTTTTTGATGTGCCTCTGCACTTGGCTTGGTGAGTCA520.11136572933844473No Hit
TGTACGGGGCAATGGGCTTTGGACCATGTGGAGGTCGTCCTAGTAAGCACTC520.11136572933844473No Hit
TCACAGGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCAA520.11136572933844473No Hit
ACTGCAGGGCGATCTCAGGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCT520.11136572933844473No Hit
CGATATGGGGATAAAGACATTAAGTTAACAAACATATAAGAAATGTATGCAC520.11136572933844473No Hit
GCAATCAGGGGAAAAGATGGAGTCAGCAGATCAGGCCGTTGCTGAGGTTGGA520.11136572933844473No Hit
AAATGAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC520.11136572933844473No Hit
AGTATAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC510.1092240806973208No Hit
GCCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA510.1092240806973208No Hit
AAGATGGGGCTGTTAAAGTATATGTGAGTCTGGTTACTACCAATTATCCACT510.1092240806973208No Hit
CCAACTGGGGTCACAGAATCTCCATTTCTGCTCAGATTCCCCCCATCTCCAT510.1092240806973208No Hit
ACTTGGGGGAATATCATGGCTATGGAACAGTAGAGAGAGACAGGCCTGATGC510.1092240806973208No Hit
AATGTAGGGACAATGTTATAGAAAAATGCCTTGAATTCTAGTGTTGTTGTCT510.1092240806973208No Hit
TAACACGGGGCTCTGCACCAAGCGGACCTAATAGACATCTACAGAACTCTCC510.1092240806973208No Hit
ACTTAGGGGTGGTTCTTCTGTTTGCATATATTAATCTGTAATTAGCATGAAT500.10708243205619686No Hit
AGCAATGGGCTTGGTCACACTGATTAAAACTAAAGATTTGTAATCTTTTTTT500.10708243205619686No Hit
ACTCGGGCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTA500.10708243205619686No Hit
GTGACTGGGCATCCACATTAAGGTGGACCCCCTCTTCACAGAGCTGCTCAAT500.10708243205619686No Hit
ATCGCTGGGCTGTACCAGGGACCCACATGACACGTGATTTGGAAAGAGACTA500.10708243205619686No Hit
GCACGAGGGTCCAACTGACCTGGGACCAGGTGTCGCGCAGAGATGTGCCTCA500.10708243205619686No Hit
ACGTAGGGGCTTCACCTCTCACATACTAAAGCATTCATTAAAAATACAACTA500.10708243205619686No Hit
TTGTGCGGGTATTGAGAGAATCGTATGATTTTTGTCTTTGACTTTGTTCATG500.10708243205619686No Hit
CTACAAGGCCTTTTGACAATAATTGCACCCCATTCCTGAAGCCTCAGCATAG500.10708243205619686No Hit
ATTAGAGGGAAAGCATGTTTTGAGTAAAATAGCTAGATTTATACTTTACTTG500.10708243205619686No Hit
CAACTAGGGCTGGCAAGCATAGGTTTTCCCAGACCACAATGCTGATATATCT500.10708243205619686No Hit
TCTCGGGGGCAGCCAAAAACCCAGAACGCTGGACAAACATTCCTTTGCTGGT500.10708243205619686No Hit
AAAATAGGGACACCAAATGTAAATCATATGGAAGTCATCTATTTGATTATGC490.10494078341507292No Hit
TTTGCAGGGAACCATGTGCATGGACAGCCATATACAGGCCCAGCAGCACATC490.10494078341507292No Hit
TAACTTTGGGATGATATGGAAAATAGAGACAGATTTGTCCTTTACAGAAATT490.10494078341507292No Hit
GATTTAGGGCCATCGCTGGGTCAATAGTACTTGCCGCAGTACTCTTAAAACT490.10494078341507292No Hit
AATCCAGGGGGAAGAAAAGTCATGGATATTTTAAAAAGAGAGTGCAAGTAGC490.10494078341507292No Hit
TAGCCTGGGGATAGAGATTAATACAACTACTTAAAAAATATAGTCAATAGGT490.10494078341507292No Hit
TAGTCGGGAAGGAAGAATCTGTGAAGCTATTCCGAGGGGTGAAGAAGATGGG490.10494078341507292No Hit
TGCAAGGGGTTCTTTTCTAAATCATAATTTTAGACCTTTTGCTATGTTCGCA490.10494078341507292No Hit
GTCCGGGAGACGAGCAGTGAAAACCTTTGTGAAAACTGAATTCTAAGGAAAT480.10279913477394897No Hit
ACTGCTGGGAGCCAGAAATAAATCCAAACATGTATGGTTAATGAATTTTTGA480.10279913477394897No Hit
TCGTACGGGGGTTTTTCTGTGTGCTTTCAACCAATCGTAGATGTTTTTGCTG480.10279913477394897No Hit
TTTTCTGGGTGTTGCCAAGGGCAGTGAGATGTTCGTAGCTTTATTTCCCAGT480.10279913477394897No Hit
CACTCTGGACTACCGGGGCGCTATGGGCTTCATCCTCATGTATGACATCACC480.10279913477394897No Hit
GCGGTTGGGAACTTGGAAATTTGTACTGAAATCCACAATAATCAGTTTTTGC480.10279913477394897No Hit
TGCAAAGGGTAGAAATTAGTTAATTATGAAAATGTGTGAAAATATAGTCACA480.10279913477394897No Hit
ACTGCTGGGTAATTTGAGGAAAATAAAGAGCAACAGAAAAGAGAACACTTGG470.10065748613282506No Hit
CTAGTTGGGTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATA470.10065748613282506No Hit
GCAAAAGGGCTGTGAAAGTGCTGCCAGCATTGATTTTTGGGGCTACAAACAC470.10065748613282506No Hit
ACTGCTGGGACTTGTGCCCGTATTGCTAATAATACCTCTTTCATAGTGCAAC470.10065748613282506No Hit
ATCTCAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC470.10065748613282506No Hit
TCAAAAGGGCAAGTGAAGCGAGTTCCTGGTCAAGGCTCATCTGAAGATGACC470.10065748613282506No Hit
CGCCTGGGGAGAAGGAGTCTAAGAACAGGCCTCACATCTGGGAGGAGCTGTG470.10065748613282506No Hit
TAACAGGGATGGTTGTTTTTCTTGCCCACTCTGGTAGGGGAAAAATCTTTTA470.10065748613282506No Hit
ACTCAAGGGAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTAC470.10065748613282506No Hit
GTGTGTGTCTTTGGAGCTATATATTGTTTTTGGAAGAGCAAAGTTTTGTTGT470.10065748613282506No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)