FastQCFastQC Report
Sat 31 Dec 2022
SRR5848115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45112
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGCCGTCT4380.9709168292250399No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGC4370.968700124135485No Hit
ATTCGAGGGAGTGCAGCTCCACATTAACTCTACAGACCAAACCATTTGTATC1100.24383755985103742No Hit
CGAATGGGGATGTAGTGACACCCATAGGCTCAAAGTAAAGTAATAAAGAAAA970.2150203936868239No Hit
TCGCAGGGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTT950.21058698350771413No Hit
AAGCTTGGGCCCCCTGAACATCCCCCTGCTTGCTGACGTGACCAGACGCTTG880.19507004788082993No Hit
TTCCACCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG870.19285334279127506No Hit
TCAATAGGGAACATAAAGCAAGTGAGCGACCTTATCTCTGTGCTTCGTGAAG820.18176981734350062No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCC810.1795531122539457No Hit
AGCTCAGGGACTTATGGTTTTATAATATATTTAATTGGATTATAATATTCCT790.17511970207483596No Hit
AGCGCTGGGTACTACAACCCTTCGCTGACGCCATAAAACTCTTCACCAAAGA770.1706862918957262No Hit
TCCTGGAGACCCGCAAGTCCTGGAGAAAGCCTGAGAAGACAGACTGAGCAGG700.15516935626884198No Hit
ACCACAGGGGGTCAAAATACAGTCTTCAGCTAATGCTTTCTTCCAGCTGGTT690.1529526511792871No Hit
GTAGCAGGGAAGATGAGAATGTTGTATTAGGTGGACTCTTCTAATCCTGAAT690.1529526511792871No Hit
CAATCTGGGCAGCTCGGAGGAAGCTTGGAGGAAACTCAGATTACTTGGTATC680.15073594608973223No Hit
AGTACAGGGGCAACATGGAGAGAATCATGAAAGCACAAGCGTACCAAACGGG680.15073594608973223No Hit
AATACCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA680.15073594608973223No Hit
GAAAAGGGAATCCATGTCGTCACAAGGATATCTGGCAAAGGAAACCAAGCTG660.14630253591062245No Hit
GTAGTTGGGCGGGCTACTACAACCCTTCGCTGACGCCATAAAACTCTTCACC660.14630253591062245No Hit
GCGAAAGGGGAACATAGAACCCAGAAATAAATCCACATATTTATAGCCAACT660.14630253591062245No Hit
CGATATGGGGATAAAGACATTAAGTTAACAAACATATAAGAAATGTATGCAC630.1396524206419578No Hit
GAAGGGGTGAGAAACTTAACTCCTTGTGCATGGCCTGGCTTGTGGATCATGT620.13743571555240292No Hit
ATCTCAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC620.13743571555240292No Hit
GCTTAGGGGATCTTACCATGTACATTATAGATGACTTACAGAATATTTTGGT620.13743571555240292No Hit
ACTAGCGGGAATGTACTGATCTATATGGTTTTATAACTCACATCTATATGGT620.13743571555240292No Hit
AGTTTAGGGCAGCCCAGATGGTCATCCATATGAGAAAATCCACACTGCAGAG610.135219010462848No Hit
GAAATGGGATACTGCCGAGTCTGGGGGCTTGTTTTGGTTTGGGGGCAGCCAT610.135219010462848No Hit
GGTACTGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA600.13300230537329313No Hit
AACGCAGGGGCTGCAAATGTTGCTTAAAGAGCCTGTCACTATCTGTGGTTTT600.13300230537329313No Hit
TGTGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA600.13300230537329313No Hit
CGCTTTGGGGCATAACTGAAAGTAGCTAAGGCACCCCAGCCGGAGGAAGTGA590.13078560028373826No Hit
TGTTGCGGGTAGACGCGGGACTTCAAGTAGCCATCAACAACAACAGAGGATT590.13078560028373826No Hit
GCGACGGGGAGGCTAAGAGTTAAGAAGTTGAACAGAACTCAGTAGTCAAAGC590.13078560028373826No Hit
AACGCAGGGATCCTTGGGGTCCTGAGGTCTTATGCAGTGGTTGGGGTCCCAC580.12856889519418335No Hit
TAAGCGGGGGGGCGTTTTCATGCTGTACAGTGACCTAAAGTTGGTAAGATGT580.12856889519418335No Hit
TTTGCAGGGAACCATGTGCATGGACAGCCATATACAGGCCCAGCAGCACATC580.12856889519418335No Hit
GCCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA580.12856889519418335No Hit
GTGTTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC580.12856889519418335No Hit
ACAATGGGAGAGTTGTGAAATTCAGCAAAGAGATTTCTACAGGAGCTAAGTC580.12856889519418335No Hit
TAAAAGGGGGGGGCAGCTTCCGGCGGGATGTTGAACTGGTCATTAATGTGTC570.12635219010462848No Hit
TATAGCGGGAGATGTAAATGAATTAACTGCTTCTATCAAAAAGCAGATTGTC570.12635219010462848No Hit
TGCTAAGGGGTTTCCAAAGTTCGTGCCTGCGAACCCAAACCTGTGAGTCTCT570.12635219010462848No Hit
TGGCTCGCAGGGTCGAGATAAGACCATCATCATGTGGAAACTGACCAGGGAT570.12635219010462848No Hit
TGTCTCGGGTGTGTGTGAAATTGGAAGCAAGGTATGTGCAAGAGGTGAAATA570.12635219010462848No Hit
GCTCTTGGGCACCACAGATCAAGAAATAGAACATTACTAGACTGGGGTATAT560.12413548501507359No Hit
TCACAGGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCAA560.12413548501507359No Hit
TCTTGTGGGAGAAGTTGGGTATACCAACCACCACCACCACCGCCCCCTATTC550.12191877992551871No Hit
GACAGTGGGATTTCAAGAAGGACAAAGGTATGTGTGCATATTTAAATAACGA550.12191877992551871No Hit
GCAAAAGGGCTGTGAAAGTGCTGCCAGCATTGATTTTTGGGGCTACAAACAC550.12191877992551871No Hit
GATTTAGGGCCATCGCTGGGTCAATAGTACTTGCCGCAGTACTCTTAAAACT540.11970207483596382No Hit
GGTTCCGGGAAACATCTTTGGAATTGTGAGTGAAGGATATAGTGGCAGGCTT540.11970207483596382No Hit
CAATTAGGGAGTCATCCAAGAGGATGGAATGATCATGTACATATTTTAGATA540.11970207483596382No Hit
TACTTCGGGAGAAATTTTCACTTACAAATGTGTATTTCCAAACATGCTAAGT540.11970207483596382No Hit
TTCGATGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG530.11748536974640893No Hit
GCGTAGGGATTGCAAGAGGCGAGTTTAAAATTCTGCAGATGGCCTTATTTGT530.11748536974640893No Hit
TAGCCTGGGGATAGAGATTAATACAACTACTTAAAAAATATAGTCAATAGGT530.11748536974640893No Hit
TACATTGGGAACTTGAAAAATACTGGTGGCGTCGATGGTGAGTGATTTTTAT530.11748536974640893No Hit
TTCTCAGGGATGCCTCATGCCCCCAGCTTCGACATTACCAAGTTCCGTTCCA530.11748536974640893No Hit
GTGTGTGTCTTTGGAGCTATATATTGTTTTTGGAAGAGCAAAGTTTTGTTGT530.11748536974640893No Hit
TTTAAAGGGGGAGGCGAAGAACGCAAAGCTGAGAACATGGACGTTAATATCG530.11748536974640893No Hit
ACTTAGGGGTGGTTCTTCTGTTTGCATATATTAATCTGTAATTAGCATGAAT520.11526866465685405No Hit
ACACAAGGGGCTCTTCCGATCTCTATGTGGGACCTCACCTTTAGTATTATGT520.11526866465685405No Hit
CTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCA520.11526866465685405No Hit
GTACTGGGGATAGTCAGGGCATTTGAGAGGAATGATTTCTTTGACGCTGGCA520.11526866465685405No Hit
ATTAGAGGGAAAGCATGTTTTGAGTAAAATAGCTAGATTTATACTTTACTTG520.11526866465685405No Hit
TGGCACAGGGAAATATGAAACTCCTACACAGGAAATCCCCAATTTTGTGATT520.11526866465685405No Hit
CAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCC520.11526866465685405No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGT510.11305195956729916No Hit
CACATTCGGGGAAATTTAAAAGATTTTTAATAGCAGTCAGGTATTTTCAGAG510.11305195956729916No Hit
TTAGGTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC510.11305195956729916No Hit
TCTGTCGGGAATAAATTTCCATTCCAACTCTCAAGTCTGTAATTTGACTCAG510.11305195956729916No Hit
GTTCGCGGGGGATGAAAAAAGGTATACCATGTTAATACAAACCGAGAGAAAG510.11305195956729916No Hit
ACCATCACCCTCTACATCACCGCCCCGACCTTAGCTCTCACCATCGCTCTTC510.11305195956729916No Hit
ACTCGGGCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTA500.11083525447774427No Hit
TTGGAGGGGCACTAGTACAGTTAAACTGACATCTTAAAGGACAACTTAAACC500.11083525447774427No Hit
ATCTGTGGGGGCAGCTGCTGATGTCGGCGCTCGCCTTCGGCTGCTTCGCTTA500.11083525447774427No Hit
GCACGAGGGTCCAACTGACCTGGGACCAGGTGTCGCGCAGAGATGTGCCTCA500.11083525447774427No Hit
GTTGCCGGGCATTAAGAATATAAAATCTCCTCTATATAGAAACAAGAATTTT500.11083525447774427No Hit
ACTTGGGGGAATATCATGGCTATGGAACAGTAGAGAGAGACAGGCCTGATGC500.11083525447774427No Hit
GATCACGGGTAAATTTCTTTGTTTCATGTATGACTCATCTTTTCTTTAGCTC500.11083525447774427No Hit
TCGCTGGGGGTTGGTGGCTGACTTTTCAGAAGGAATTATTCACCTCACATGA500.11083525447774427No Hit
ACTGCCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC500.11083525447774427No Hit
GCCAAAGGGCAAATACCAATGAAATGTAACTTTTAAATATTCCTCACTGTGT490.10861854938818939No Hit
GCTCATGGGTATTCAGAAAGGTTAAGCAGTGCAGGGCGTCATGTGCTTACAG490.10861854938818939No Hit
TGTACGGGGCAATGGGCTTTGGACCATGTGGAGGTCGTCCTAGTAAGCACTC490.10861854938818939No Hit
TTTGCAGGGATTGACACAAGTTATCAAAATCTCTGGAATATAGTAAAAGCAG490.10861854938818939No Hit
TCCACAGGGCACTTTTGACATTGATGCCAATGGTATACTCAATGTCTCTGCT490.10861854938818939No Hit
TGCCAAGGGATTCGAGACCTCCAACATACTTGTCTGAAGGTGGTGATTCTGG490.10861854938818939No Hit
GCAGCAGGGCCTGCCCACTCCCTACGTGACTTTGATCAGGTCACTAGTGTCT490.10861854938818939No Hit
ATCACAGGGGGGTGCAGTCGTCACTCGCGTCTGGCTACCAGCTCCCCGCTGC490.10861854938818939No Hit
TTTTCTGGGTGTTGCCAAGGGCAGTGAGATGTTCGTAGCTTTATTTCCCAGT490.10861854938818939No Hit
CTTAGAAGGGATATACTCTTTTTGGCATCTTTCATTCAGCATAATTGTTCTA490.10861854938818939No Hit
TAGTCGGGAAGGAAGAATCTGTGAAGCTATTCCGAGGGGTGAAGAAGATGGG490.10861854938818939No Hit
AGCAATGGGCTTGGTCACACTGATTAAAACTAAAGATTTGTAATCTTTTTTT480.1064018442986345No Hit
ACACAGGGCATTGTCTATGAATTGGACAAGAACTTGAAGCCTATCAAGCCCA480.1064018442986345No Hit
CCGACTGGGGTAGAGAAGTTGTAGTCGAAGGGAATGTTGGGACTCCTCACTG480.1064018442986345No Hit
GGTATAGGGAGACTGGCGCGCGCGGACGGTCATGGGACTTCAGCATGGCGGT480.1064018442986345No Hit
AATGTAGGGACAATGTTATAGAAAAATGCCTTGAATTCTAGTGTTGTTGTCT480.1064018442986345No Hit
ACTCAAGGGAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTAC480.1064018442986345No Hit
AGTTGGGGGGTATTTAGCATTGTAGAAACTGTGAAGAATCTGCACATACTCC470.10418513920907961No Hit
CATAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC470.10418513920907961No Hit
TGAATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA470.10418513920907961No Hit
ATTGCTGGGTTACTACTCCGGAAAAAAAGAACCATTTGGATACATAGGTATG470.10418513920907961No Hit
CATTCGGGGGGGTCTGCGCCGGAAGTGCATGAGCTGCCGATGTGGTGCTTAG470.10418513920907961No Hit
ACGGCTGGGGCTTACTGGGGGCTGAAGGTTCTGGACATGAACAAGGGTCAGG470.10418513920907961No Hit
TGGTAGGGGAGTCCAGGGAACCATTTACTTCTTTTGGAGTAGATAGTTATCC470.10418513920907961No Hit
GTGCTTGGGTCTAGTGACTTTTTAAAGCCCTAACGTTTACATAAAGAAGCTC460.10196843411952475No Hit
CCAACTGGGGTCACAGAATCTCCATTTCTGCTCAGATTCCCCCCATCTCCAT460.10196843411952475No Hit
AACGCAGGGATGTGCATTTGGTATCATGGGCCGTGGAACAGAATATATGTTG460.10196843411952475No Hit
ACTGCAGGGCGATCTCAGGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCT460.10196843411952475No Hit
TCAACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA460.10196843411952475No Hit
ACTATAGGGTATGGTATCTCTCTAGATTTCAAGGAAACACACTGGATACTGC460.10196843411952475No Hit
TTGTGCGGGTATTGAGAGAATCGTATGATTTTTGTCTTTGACTTTGTTCATG460.10196843411952475No Hit
CAAAGAGGGGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGA460.10196843411952475No Hit
GATCATGGGGTCTGAAAGAACAGAAGGCATCAGAGTTTCCAGTCACTGAAGA460.10196843411952475No Hit
GCGCTAGGGGTTCAGGCTACTGAAACATTAAAATGTGAATTCCCAAACTTTT460.10196843411952475No Hit
TCGGCCGGGGGTCAAACGACGAGATTGTGAAGAATCTAATATGGATATATTT460.10196843411952475No Hit
AAATGAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC460.10196843411952475No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)