FastQCFastQC Report
Sat 31 Dec 2022
SRR5848103.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848103.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156761
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGCCGTCT11250.7176529876691269No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGTCCCATCTCGTATGC8820.5626399423325955No Hit
CATCAGGGGCAGAGCGAGACTCCATCTCAAAAAAGTAAAATACAATAAAATG2270.14480642506745936No Hit
TAACGCGGGTGCCTTTCTCAGAATCACATCCTCATCAGCCACTTGACAACAC2180.13906520116610638No Hit
TCTGAGGGGCACACAGATAAGTGGCTCTTACCCAGCTCTCAGTGACTCCCCC2020.12885858089703434No Hit
TTTAAAGGGGATGTGAAAAAGGAAAAAGGAAAGGTAATAAACAAACAACCAA1990.12694483959658334No Hit
TAGACGGGGATTACAGAAAAAAATCTGAACTGAGAAAGGAGCTCTGAATTTA1930.12311735699568133No Hit
CGTTGTGGGTTGACTAGCAAGACATCCTACACATACTGCTTTTCATGAGGTA1920.12247944322886432No Hit
AACGCTGGGATGTGTTGAAAATATATGTTGTGAGAATTTATCATTGTACAGC1880.11992778816159631No Hit
TCTAATGGGGTACTGAAAGCCTCTTCAAATTTTCTGTCTGTCGAGTTATTTT1860.11865196062796231No Hit
TGTAACGGGTACCACATTTAAAAAAAACTAGTCTTCTATTGATGGACCTTTA1850.1180140468611453No Hit
TTTGGTGGGCCGATCTGTCTTGGGGATTGACGTTTTCAGTTTTCATGAATGT1840.11737613309432832No Hit
TTTGCCGGGGAGAGTGGAGCGGCCGCCGGAGATGCCTGACGCATCTGTCTGA1840.11737613309432832No Hit
GACTAGGGCGATCTGCGTAGGGACTGTTGGATGTGTCCATTGTCGCCAGTTT1820.1161003055606943No Hit
ATAGTCGGGTAAGGAAGAAGGAAAGAATGTCCTGTCTCACCACTCCTATTCA1800.1148244780270603No Hit
TGTTCTGGGATAGAAGATTGTAATTGTAGAGAAATATTTCTCTTAGAAATAA1780.1135486504934263No Hit
GTTTCCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1760.11227282295979231No Hit
CAGTACGGGACCCTACTGTGTCCTACTTAAGTGGATGCCATTTTTAGTCTCT1750.11163490919297528No Hit
AAAAACGGGAGCAAAAGAAACTATCAACAGAGTAAACTGACCACCTACAGAA1730.11035908165934129No Hit
GGCTAAGGGAGTAAGATTTGGATGATATTTGTCACTTTCTGCCACCTCAGAG1730.11035908165934129No Hit
CGCTCTGGGACCAGTTGCCTCTCCAGCAGCCCTTGGAAGCACTGCTACAGCA1720.10972116789252429No Hit
TGAATAGGGAAGCAAAACTATCTTTAGTGACTTAAAAAATAATATCCTCTAA1720.10972116789252429No Hit
ACGCTTGGGGAATTGTATTTACAAATATATTTTGTTGAGTGGCTTGGGTGCA1710.1090832541257073No Hit
CTTTTAGGGCACTAAAACTGCACAACAACATGGAAACTGAACAACCTGCTCC1700.1084453403588903No Hit
CTCTTGGGGATCCCATGCTCATGGATGGGTAGAATCAATATTGTGAAAATGA1700.1084453403588903No Hit
CCTAATGGGCGATCTATAGGCGGGTACTAACTACCTGACTCCTACCCCTCAC1680.10716951282525627No Hit
CCATGAGGGCTTGCTTAAAATCAGTTGACCATATATGTGAGGGTTTATTTCT1680.10716951282525627No Hit
CACTTTGGGGCAGCACATGCTCCTTGCGTAAAACCACATTAAATAATTCCAG1680.10716951282525627No Hit
TCACCAGGGTATAAAAGGAACCTCATCAGGCTAACGACAGACTTCTCAAAAG1660.10589368529162228No Hit
ACTCATGGGATCTCTGTTTTTCTTAACTCAGAACTCAGGCTAAAAGAGTGAT1660.10589368529162228No Hit
AGCACAGGGCCTGGCCTCCAACTCCAGAATTGGAGCCCTCCAGACATTACAA1660.10589368529162228No Hit
AAAGAGGGGTCCGGGGCCGTGGCCTGGGCCTGATCATGGCTCTAGGGGCGCT1640.10461785775798828No Hit
ACGCTTGGGCTTTTGAGAATAGTCAAGGACATAGAAGCAGTACATTCTGAAC1630.10397994399117126No Hit
AGTTGCGGGTTGACATGTCTTCCGTGGTGGGGGCAGGGGAAGGCAGAGCCCA1620.10334203022435426No Hit
GTCAGGGGGATCTCTTAAGCTGGATCCTAGCAGATCTGAGAAAGAAAGGGAA1620.10334203022435426No Hit
GCGCGAGGGAAGTAGGATAAAGTTTTTTTTTCTTTTTACAAACGTACAGTAA1620.10334203022435426No Hit
GCGGCGGGGATTGGATTCATGACTGTGCAGCATTTTTAGTTATGTGGTGTTT1610.10270411645753727No Hit
AATGAGGGGGATTACATAAATATGTAGGAAAAAACATATTGAGTTCAAAATT1610.10270411645753727No Hit
GCCTTGGTCTCCCAGGGTGCTGGGAGTGCAGGCGTGAGCCACTGCGCCTGGC1600.10206620269072027No Hit
GTTTGCGGGGTTAAAAATGTGGATAGATACTGTGAAGCTGCTCGCTCAGAAA1600.10206620269072027No Hit
GATCGTGGGCCTGAGGAAGTTTACTGTCATGAGTCTCGGCTGGTTGGCGCAT1590.10142828892390326No Hit
CAAAAAGGGGTATTATGCATTGGAAAGGTATTTTTTTTAAGTTCTGTTGGCT1590.10142828892390326No Hit
CTTACTGGGTGAAAAATTTAGGAGTAAATTTGACAAAATATGTGTAAACCTG1570.10015246139026927No Hit
TAATCAGGGTACCAAGAGTTTGGCCAGTGTGTGGGAGACTTGAACACCCCCC1570.10015246139026927No Hit
AGGTGAGGGGTTCCGACCCCTTTCCCAGCCCACAGGGCTTGAAGTGAAGGAA1570.10015246139026927No Hit
GGCGAGGGGGGCGGCCGCGGAGTATCCTGGAGCTGCAGACAGTGCGGGCCTG1570.10015246139026927No Hit
GTCTTTGGGAAGATTCTATCAACTTTTTATTCTACTAATGTAGCTCCATGTA1570.10015246139026927No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)