FastQCFastQC Report
Sat 31 Dec 2022
SRR5848086.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848086.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140335
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGCCTGTATCTCGTATGC3620.2579541810667332No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCCTGTATCTCGTATGCCGTCT3580.25510385862400686No Hit
GTGACGGGGAGGCAGCCATGGTGCGGTTCAAGCACAGGTACCTGCTCTGCGA1770.12612676809064027No Hit
GAACCTGGGCTCGATCTCCTGACCTTGTGATCCGCCCGCCTCGGCCTCCCAA1720.12256386503723234No Hit
AAAAAAGGGCATCCATGCCAACTACCTAACTCGTTATCAGAGCTGATAGAGC1670.11900096198382443No Hit
TTCGCCGGGGGAATGCTTACTAAATAAGACAATGGGAGCTTTGCTAAAGCAA1660.11828838137314283No Hit
GCAGCAGGGAGAGTGTGCCTTACAAGGAAACAAGGCAAATACAGGAGGAAAA1650.11757580076246126No Hit
CTCTGTGGGGGCCTCTGTTTGGCGCTTTTGTGCGCGCCCGGGTCTGTTGGTG1620.11543805893041652No Hit
GTTTTTGGGCACCTATCATAATGGTCTTAGTCATTTCACACAAATCAGAACT1610.11472547831973491No Hit
GACTAGGGGGTGGGGACGTAGAGCCAAACCATGTCAATATTCATTTGAAAAT1600.11401289770905333No Hit
TCTTACGGGGTAGACCTATGTTTTTATTTCTCTTGAGTAAATACCTAGAGGT1600.11401289770905333No Hit
CACAGTGGGCTACAGCAGAAAGCTTTCAGTCTAGTGAGTAAAGAGGCAGTTT1580.11258773648769016No Hit
TTCTACGGGCTCTGATAAAGATGGACATTCACAAAAAAGTAACTGACCCAAG1580.11258773648769016No Hit
GCTACAGGGCTCCTTATGAACGATTTGGCATTTCTCTCACCTGGATAATTTT1570.11187515587700858No Hit
GAAAGGGGTCCCTGGCTGGACCAAGCCCATCACCATTGGCAGGCACGCCCAT1560.111162575266327No Hit
CGTCCAGGGACCACTGCTGGTGGTTCACACATCATCCCATCCTTAATATGCC1540.10973741404496384No Hit
ATACAAGGGAGGATAAATCAAGATTCTGTTACTTCATCTTGGCTAGAAGCAG1540.10973741404496384No Hit
TAAGTGCGGGACATGGAACGCTTTCATAAACTTCAGGGATCCCGTGTTGCCA1530.10902483343428225No Hit
TGACAAGGAGGGCTATGGCCGGAGCTTGAGATACGCCGCTCTGAATCTTGCC1520.10831225282360067No Hit
GACTAGGGGCAAGTTTCATAAATCTTTCTTCCTTTTAGTATTATAAGCCCAT1510.10759967221291909No Hit
TGTGCCGGGGGGCTGTGTTGCTTCTATGCCCAGCAGTATGATATGACTCTGA1500.10688709160223751No Hit
CGGATGGGGTTCTTTCGTTTGTACAGGGTCAAGTTATCTTCCATCTAGTCAA1490.10617451099155593No Hit
AGCATGGGTTACAACTCCAAAGTGAAATTAATCAATTACATAGCAAACTTCT1480.10546193038087433No Hit
ACAGGTAGGGTGAAGTGGCATGTTGGCCTTTTCTTTCTTCCTGCTCCCTGCA1470.10474934977019275No Hit
GGATTGGGGGCTCCTCTCCCAGTCGGCACCACAGCGGTGGCTGCCGGGCGTG1460.10403676915951117No Hit
AGGTCTGGGGACACAGAGAGCATAGTGTGTTTTATGAAACTCCTAAGCAATT1460.10403676915951117No Hit
AATTAGGGGGAACAAAGCCCGTTTAACAACTGCAAAGCACACAGTCTGCAGA1460.10403676915951117No Hit
CTTAGTCTGGGGGCGTGGACGGTGTATGAAGAGATGATGGCCAAGATGGGCC1460.10403676915951117No Hit
CAACTAGGGAACTCTGCAGCCTTCAAAGCTCGAACCAAAGCTAGAAGCAAAT1460.10403676915951117No Hit
TCGCTTGGGAATGAATATTGATGCGAAAATCCTCAGCAAACTACTAGCAAAG1450.10332418854882958No Hit
CACGAGGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACA1420.10118644671678484No Hit
TGCCGGGATATTAAGCATTCTAACAATGCAAATGTTCCTGCTAATATAGCCA1420.10118644671678484No Hit
TGAAAGGGGGAGTAACTTTTTACAGGAATGTTTATTATCTTTGTCATCCGAA1410.10047386610610326No Hit
GCGCACGGGTATCTTTAAAAGGTTAGAGGCAACTTCACTGAACTTGGGAGGT1410.10047386610610326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)