FastQCFastQC Report
Sat 31 Dec 2022
SRR5848082.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848082.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120466
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGCCTGTATCTCGTATGC3950.32789334750053956No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCCTGTATCTCGTATGCCGTCT3920.32540301827901646No Hit
CAACAGGGGTGTCTCACACTGATGATACCTCACATGTTGACCGGCTATGTGA1810.15024986303189283No Hit
TCTACAGGGAAGCTTTTGGCATATTAATTTGCATTTTATTGCTACTCTTTCT1790.14858964355087742No Hit
TGCACTGGGGTGCTGCTGTTCTGCTACTGGAGAGGGTGAAGTCACTTTCAAC1740.14443909484833894No Hit
CAACAAGGGATCTCTGTTTGCTGATGATTTAAGCATATACTTAGATAAACCT1670.13862832666478508No Hit
CTTGCTGGGATTTGGATGGGAAACATGTGGTGTTTGGCCAAGTAATTAAAGG1660.13779821692427738No Hit
TATCGCGGGTCAGGGAGGTTTTGCTCCAGATTACTATGATTGGGGACAACAA1630.1353078877027543No Hit
GATTAAGGGGATGATTATAAAATGCTTAGTATGAGTCTTCATGATCTTGGAT1610.13364766822173893No Hit
GATGGGGATGATAGAAGCAAGACCAAATGATGCTACCTATTGTGATAAATCT1610.13364766822173893No Hit
TCAATCGGGAGTGTCAGAATTTATAGGCCTCTTTAACAGGGAAGAGACAAAT1600.1328175584812312No Hit
AAGACAGGGACTGTAGAGTTATAAAAAATGTGGTTACTCTTTTTTTTTTTTT1580.13115733900021584No Hit
ACCTGCGGGAAACCAAAAACCTTTACTGTTTCTAAATTTCCTAGATTGCTAT1580.13115733900021584No Hit
TGACGTGGGAACTACAACAGCCCAATCTGGTCAGGACTACAAATGACCCAGG1580.13115733900021584No Hit
GTCGTAGGGAACACCAAGCTAGTGTATGTGCAAATATTTCTCTAAATCATTA1570.13032722925970813No Hit
ATAACTGGGAGATGCTTGCGGCAAAAGGAAAGCTGTGTTTATATGGAAGAAA1570.13032722925970813No Hit
GTAACTGGGTGGAAGTAGATAGTGGTTATGGTTGCACAATCTTGAAAATATA1560.12949711951920043No Hit
TTGGCCGGGAGATTCTTCTTCCAAAAACATAAAATGAAAGCTACAGGTTTTT1550.12866700977869275No Hit
CATTAGGGGGGGCAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTC1540.12783690003818504No Hit
TAAGACGGGGTGAGCTTGTAAGTATCTCTCTAAGCCCAGAGTCTGACTATGA1520.12617668055716966No Hit
TAAACGGGTGTATTATTACCTGCAAGAATACATGATAGACAATGTATAAACA1520.12617668055716966No Hit
TCGCTTGGGGTGTTTGCATTGTCTAATGACAGATAAATCCTTAACATTTCTC1510.12534657081666195No Hit
CTCAAGGGGAATAAATTGTTCTAGCATTTGTATAAGCCTTTACAAATGTCAA1510.12534657081666195No Hit
ATTGATGGGTCTCTTGAGAACTATATTTTGGTTCCTAGTAACAGCCTTTCCA1500.12451646107615427No Hit
ATAGTGGGGGACCCGGTGGTGTTCCCGGGAGGAGAGGGCGGAGGGCGGAGGG1490.12368635133564658No Hit
CGCTACGGGGTTCTCTAGATTATTTGACTTCAGTTTCTCTGCCTTCAGGTAA1480.12285624159513886No Hit
GGTTATGGGCTCTTGGAGATTGTTGAATGCAGCCAGTGAAGAAGTAGATTCC1480.12285624159513886No Hit
GGCATGGAGAATCCACCCAAACACCTTTAAAGGATAGGTGTCTGTTCATAGG1470.12202613185463118No Hit
TTTAGGGGGATCACATTCTTAAAGGAATTATTAAGTGCTGTTGTTTTGACCT1430.1187056928926004No Hit
TGGTTCGGGGGGAGAGCACTTAAAAAAGGAGAATACAGAGTTAAAGTATACC1430.1187056928926004No Hit
GTTCAAGGGTAATCTCTAGAGATTAAAGAAAGATCCAGACAAATCTTGCTTG1430.1187056928926004No Hit
TAATGGGGGCTTCCAGATTTTAAAGGAGAAGAGGATAATTGTAGATTGGGCT1430.1187056928926004No Hit
GTTTAAGGGGCCCTGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAAC1420.1178755831520927No Hit
TTGCACGGGGCGCCTAAGATTGATGTGATCCTTGACTTTTTAAAGTACCTGG1410.11704547341158501No Hit
ATATACGGGATTGAGCAGGCCATCAAAGAGCATTTGAATCAAGGCAGCTCTC1410.11704547341158501No Hit
TCAACTGGGATCTTTTTGATATGCTGCTTGATTTGATTTGCCACTGTTTTTG1400.11621536367107732No Hit
TCTAGTGGGGAACCAGTGAAACTTGAACCAATGACTTGGCTAGTTCAGCACA1380.11455514419006192No Hit
GTTTCTGGGAGAACACCAAAGACAAAGGGAAGTCCTAAAAGTTGACAGAGGA1370.11372503444955423No Hit
ATTAGTGGGATGCAAGAAAAGGAGTGAGGAATGGGAAAATTGGAAGAACGAG1370.11372503444955423No Hit
TTGCCCGGGTGTCCTGCCTGTGCCCCTTACAGTTCTGGGCGATGGCCGGTCA1370.11372503444955423No Hit
GCACAAGGGAAGTATTGACTATTAGAATGTCTTGAACTTTGGTTACTGTGAG1360.11289492470904655No Hit
ATAAAAGGGTCAGCTGTCCGGGCTGCTTTCGCCTCCGCCTGTGGATGCTGCG1360.11289492470904655No Hit
GTGCTGGGAAGGCGTCTGTAAAAGAAGATATCTTTATTGGAGCAATGTTCAT1360.11289492470904655No Hit
ACACCAGGGGTAATATCAAGAATCTGTAGGGAACTTAGACAAATCAGCAAAC1360.11289492470904655No Hit
TCCGGTGGGATATCTTAGATGTGTAATTTATGAACTACTACTGGATTATTTT1350.11206481496853883No Hit
TTATAGGGATCCTAGGGCCTAATAAGAAGGATGGGCATAACCTCTAACCTAC1350.11206481496853883No Hit
GCTAATGGGATGGAGGATGTTGAGTACCTTTGCATGTGCTCATTGGCCTTTT1350.11206481496853883No Hit
ATATGTGGGATACAGGAGCACCCAGATTCATAAAGCAAGTCCTGAGTGACCT1350.11206481496853883No Hit
ATTTCTGGGGTTCAACAGGAGCTTCAGTCAAAAAGGGCTCGGAGCTCAGGGA1340.11123470522803114No Hit
GTGATGGGGAGAGACTCCATGGTTCATAATATCTTTGTGGTCATACTACATT1340.11123470522803114No Hit
TAATAAGGGTTGGAAGAAAGACTGCTGCCACGCGGTGCAGAAGTTTCTTTCC1340.11123470522803114No Hit
TAAGTAGGGATCATCTACAGATCTTCTGGTCGCTTCCTTTCCTCCACTGATG1340.11123470522803114No Hit
GTATCCGGGAACTAAAGAGCTTCTGCACAGCAAAAGAAACCACCATCAGAGT1340.11123470522803114No Hit
ATAAGAGGGGCGTCGCTTTCAGCCTCTGGTGAAGGGCGGCGCGCTTAGGCAG1340.11123470522803114No Hit
CTGTCAGGGGATGATTTTCTATCCAGCAATTTTACTGAAGTCATTTATCAGT1330.11040459548752346No Hit
GACATTGGGGTAGCTAAAACAGATCAATTCAGTCATAATGTTGGGATGGGGC1330.11040459548752346No Hit
ACAATAGGGAAGGCTGTTGTGATCTGTGACATATGGGTTATATTATTGTGTC1330.11040459548752346No Hit
CGGCCGGGGATTAAAGTTGGTTTTAGCAAGGATTAGGATTACTACAATTATT1330.11040459548752346No Hit
CAATCTGGGACCGGAGGGAGGAGGGAGGATGCGCTGTGGGGTTGTTTTTGCC1330.11040459548752346No Hit
TTAAAAGGGGACACTGATGAACACGCACAAGCATCAAGACCACCAAGGAAAA1320.10957448574701577No Hit
AGCGCAGGGAGAGAAGAACAAAATACTAGAAGCACTTGCCAGCTCAGAGGAT1320.10957448574701577No Hit
ACAAGTGGGTGCTCTCGGTGAGTTATCACAGAAATGATGTTGCAGTTATGGT1320.10957448574701577No Hit
GCCGCAGGGCCCAAAGGGTTTTGGGTATAGCCTGCCCTGGAGGGGAAGGAAG1310.10874437600650805No Hit
AAGCCAGGGGAATTTGTTTACTGACTGGTGTTAAAATGAATGTGCTTATTAC1310.10874437600650805No Hit
TCTGCCGGGATGACACTGTATTAAAACTGTTCACATCTAAAATAAGAACTTC1310.10874437600650805No Hit
AGAAGAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1310.10874437600650805No Hit
GCGCATGGGAAGCTTTTATCTTTTAACCAACTGAACAATACACCAAAAGCAG1310.10874437600650805No Hit
TGTCCAGGGACTGTAAGGAAGAGCTTTTCCATCACCCCCACTTTTTGGTCTG1300.10791426626600037No Hit
TCACTCGGGGCATGAGAGAATCCACACTGGTCAAAAGCCTTTTGAGTGCACC1290.10708415652549268No Hit
TGGCCCGGGGATAAACTCAAGAGAATAATTTTTATAAACCCTGTTTTCCAAT1290.10708415652549268No Hit
ACAACAGGGACACCAAAAGCAGCCTAGGGATGAGCATTTCTTTGAAAGCAAT1290.10708415652549268No Hit
AGTTATGGGGGGAATGATGACGGAGCGAGAAAGCGAGACAATTTAACACGGA1290.10708415652549268No Hit
GCTTAGGGGGCATACTGTTGAAACCCGCAGTGTTGCATTTAGAAAACAGTTG1290.10708415652549268No Hit
CGCCTAGGGATTGGCGTTTGAGTGAGCCAAGAAAATGCGTATAAATACACAT1290.10708415652549268No Hit
ATGTATGGGGATCTAGTTATGGGAGGAATATTACAGCTGTAAATTATATGAG1290.10708415652549268No Hit
AAAACAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1280.10625404678498498No Hit
GTTTCAGGGATTGATAGTGGAGATATATTAATTTTTAAAACTGTAAAGTAAA1280.10625404678498498No Hit
AAATAAGGGTCCATATGCTTCTATGTTTTTCACACAAATAGTATGCTACTAT1280.10625404678498498No Hit
GTGCTCGGGTAACCACTCCGGGGTGCCCAGAGGGAGGGAGAAAGGTTCACTT1280.10625404678498498No Hit
CACATAGGGTGGCAGCAAAAATAAACTGTCAAGTAGTAAGAAGAACTTGGAT1280.10625404678498498No Hit
TTCCCGGGGCCCGGACCCACGTGGAAGCGGCACTCAAGATGGTAGGAGAATG1280.10625404678498498No Hit
TGCATGCGGGTCCGATCTATAGTGGGGATTGTGGACAAAGTTATTATTTTGC1270.10542393704447728No Hit
GTTAAGGGGTCCTGATCTGTGATAGTTCACCCCCTGAGATCCCCTGAGCCCC1270.10542393704447728No Hit
CCTAGTGGGGACCTCTTGCTGTCTCTGCTCGCCCTCCTTTCTGTGCCCTACT1270.10542393704447728No Hit
ACCTATGGGCAATGATTTCCTTATACTTATCCTGGATTTTAAATTTGGAAAT1270.10542393704447728No Hit
CATGTCGGGGTAGAATATAAGTGGTGGATGCAGAAGTGATTGCTGTAAAATT1270.10542393704447728No Hit
TATACTGGGGCCTCATCTTGCCATCTCCCGGCCAGGCTGGGAACGGGCACCA1270.10542393704447728No Hit
TAACACGGGGGTGACCGGCTTGGCGGTTGGAGCGGCTGTCGCCATGTTGTCG1260.10459382730396959No Hit
AATCGTGGGAGGAGGTTCCTGCTGTGCCAGAAACCCTTAAGAAAAAGCGAAG1260.10459382730396959No Hit
CACTAGGGGAACACAGAGTCACCTGTGACAGGCTCTAGGTTTGAGCTAGACT1260.10459382730396959No Hit
AAGCCTGGGCGAGGAGAGCAAGACTCTGTCTCAAAAATAAATACATAAAATA1260.10459382730396959No Hit
TCGTAGGGTATAACCTATTTTCTTGTGGAAGCAATGTGAGAAATAGGACTTG1260.10459382730396959No Hit
GCCATAGGGGATCTTATCGTGGGACTAAAAATCCATCCATTACATTGGCAAA1260.10459382730396959No Hit
CAGTAGGGAGTTCTGTGACTTCATGTTTTAACTAAGAAGGAAAATTCATAGA1250.1037637175634619No Hit
GCTTACGGGATACTATTTAAGTCTTTTATGGTTATTTCAAGTATACAATTTC1250.1037637175634619No Hit
GAAAGAGGGAATCTCATGAATAAATAAGTTGATCCATTAGTTAGATTTTCAT1250.1037637175634619No Hit
ACAGAATGGGTATGGTGGGGTGAGGGTGGTTACACCCTGAAATTTAAATGAT1250.1037637175634619No Hit
GGTTCAGGGGTAGATTTAAATGCTCAAAAATAAATGTAACTGAGAAGAGTTA1250.1037637175634619No Hit
GCTGTCCTTTTTATTGTTTTTAATTAATATTTTTGTTTTAATTGATAGCAAA1240.1029336078229542No Hit
GCCTCGGGGTGCCGCAGTAGTTCTTACTCCTATGGAACCAATGATAGAAATG1240.1029336078229542No Hit
AACGCTGGGTAGCGAATGTCTTTTAAGAGGACCAGCCAGACTAACATGGTGA1240.1029336078229542No Hit
TTCCCAGGGAATCACAAATTTTTGGCTAATTTTTAACCTGAGGTTTTGTTTT1240.1029336078229542No Hit
CATCATAGGGGGACCTGAAAAGAAGCTTTGCCTGGAGGAAACCTTACCTGAC1240.1029336078229542No Hit
ACACTGGGTGACGATAGTGTTTCTAATATTTTGTATTTTTATTCCCCTCCCC1240.1029336078229542No Hit
TCAAATAGGAAGGGAAATATTTTTGTAAATGTAATGGTTTTGAAAATCTGAG1240.1029336078229542No Hit
TTTACGGGGGAGAGCCCCAGTTCAAAAACCTATTACATAGACCACATTCAGG1240.1029336078229542No Hit
ACTTTTGGGTGAATTTTACTTTAGAATTGAGCCCCTGTGCAAAATGTAATTC1230.1021034980824465No Hit
TGTAGAGGGAGTGACATAAGTAGCAGCCGTTTTTCAACTTCAGTTTCATTCA1230.1021034980824465No Hit
GTACATGGGGATTTAAAAACAAGCAAAGAAACAACACCTCAGCAGCTGCCCG1230.1021034980824465No Hit
AACACAGGGGCAAACATGGAATGCCTGTAAGAGTTATTGTAAGTTTTCCATT1230.1021034980824465No Hit
GCATTGGGGATATATCTAGTGACTACAGTCTTTGACATCACAGATGAAGAAC1220.1012733883419388No Hit
TCTCATGGGGAAAACAATTCACTTATAAACTTATAAAACCTGTTAGGGATAA1220.1012733883419388No Hit
ACACAAGGGAGAGCGACGCAGCCTTCGGTGCAGTCGTCACTCGCGTCTGGCT1220.1012733883419388No Hit
CGCACTGGGCGTACAACCCACTCTGCCTAGAAGGTGTGGAGGACTCACCACG1220.1012733883419388No Hit
TAAATCGGGAACTTTCCTTTTTTATAGGAGTTGCCTGCCTTAACTTTTCAGC1220.1012733883419388No Hit
GTACCTGGGAGTGAGAAGAGAGAGACTCATCCCAAAAGAGGCCGCCCTGGAA1220.1012733883419388No Hit
GGGTTAGGGGGACACGGCGGAGCAGAGAGAGATGCTCGGCCGCTCCTCCTCC1220.1012733883419388No Hit
GACTTAGGGCAAACTCCTGACCTAAAGAGATCCACCTGCCTCGGCCTCCCAA1220.1012733883419388No Hit
TGTGTAGGGCAAGCGGAAGCATGAGACCCGCCCACACTAGCCTCTGTGTTCC1210.10044327860143111No Hit
TTCATAGGGAGCCACTGGACCCCGTGCTGTGCACCCTGCGGCCCCGCGGCTG1210.10044327860143111No Hit
TCACGGGAAGCACACTGTATGTTCAGTAGGTTGAACTATGAACACTGTCATC1210.10044327860143111No Hit
TAGACAGGGGTTCTTTTTCTGTTCTACAAAGTGTGTGTATATGTGACTATTA1210.10044327860143111No Hit
AATTCAGGGTGTGTGGATACATGTAATACAATCCGTTCAAAACAAATGTAAA1210.10044327860143111No Hit
TTGCACGGGGTCATGTTTTTCGGAAGGCAACTCCATACACTTTTCCTGGTGG1210.10044327860143111No Hit
CTCTCCGGGACTCTTGAATTTTTCAGATAATTGAGTAACATAATAGAAGAGA1210.10044327860143111No Hit
AGCCTGGGAAACAGAGCGAAACCTTGTCTCAAAAAAAATCAAAAAAACGGGA1210.10044327860143111No Hit
GCACGCTGGGGATTTTTTGAGGATTTTAGTCAACTAATGTTTAAGGCTACTT1210.10044327860143111No Hit
GCCTATTTTCTACTCTCCCTGAGCAGAAATTTAAGTGTCACATTTTGCCAAG1210.10044327860143111No Hit
GTAAAGGAGGAAGCTGTGGCAGAGGTGGTCACCATCACCAAATCGGTAAAGG1210.10044327860143111No Hit
TTTGTGGGGCCACACTAATGGCAGGTTCTCTTAGTCCCTCTAGCTCGTCCTC1210.10044327860143111No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)