FastQCFastQC Report
Sat 31 Dec 2022
SRR5848081.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848081.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124635
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGCCTGTATCTCGTATGC3540.2840293657479841No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCCTGTATCTCGTATGCCGTCT3400.2727965659726401No Hit
GGATCCGGGACTTGCAGATGGTTGAAAGATAGCTTGAATTTAATGAAATAGA1610.12917719741645606No Hit
TAACCGGGGTCAAGAAATGTTTGCTTAGTCCACTGTCCAAGAGAGTTTTCCC1510.12115376900549604No Hit
ACAATTGGGCAAGCACAACTGACATTCACTGTTTTAAAAAACAAAAAAGAGA1500.12035142616440005No Hit
AACGAAGGGAGCTGTTGGTGGCTTTAGTCCCAATGTGAATCATGGAGAGCTC1480.11874674048220805No Hit
TAAGTGCGGGAAATCAGCTTTGGAAAGTAATTTAAATGTACCTTATTTTTTT1430.11473502627672805No Hit
GACTTAGGGGAATAATAATATTATGATTGTAAAAGATTAAGAAAGCCATTTC1420.11393268343563205No Hit
GCTGGGATTACAGGTGTGAGCCACCGCGCCCTGCGTAGTCTTTTGCTTTAAA1420.11393268343563205No Hit
CGAAAAGGGATATTAGATTGCTCCAGAACAAGGATTTTTATGGGGCCTATTG1400.11232799775344005No Hit
ATCATCGGGCGCCTCACTGGGCGCTCTGCTCTTCACCTGCTTCCTCGCAGTG1390.11152565491234405No Hit
ATAGCTGGGAAAATTCCCCATGTTCACATACCAAAGGGTTACTTATGAGCTG1360.10911862638905605No Hit
CTCAGTGGGTGTTAGCAAGGCTATGTATTTGTAAATGTCTCCTACTGCCAGA1360.10911862638905605No Hit
AATCATGGGAGGACATTTTGGCGCAAGTTTTACAGATTTTATTAAAGTCGAA1350.10831628354796004No Hit
GACGCTGGGGGGAGGTGGGGCCTTCAGAAAAGTGATTAGATGGTAGGGGCGG1350.10831628354796004No Hit
AAAGGGGAACTCCACCAGGTTCCCACAGTAAATATGAGAGAAAAGTTCCTTC1350.10831628354796004No Hit
CGTACAGGGGTGTTGTAAAAAAGTATTTTAAATCACATTCAAATTTCAGTGG1320.10590925502467204No Hit
TCACTGGGATTTCTTTTTTAGTTCAAAGGTGAAATTTTGACCTTTGTATTAA1320.10590925502467204No Hit
GCAGGCGGGACACAGGAGAGGCACTCAAACTAGGTTGGAAGGTCAGGGAAGG1320.10590925502467204No Hit
TCCAGTGGGAAATCAGGCTTGTTTGTTTTCCTAGAGTATTAGTTTCCTATTT1320.10590925502467204No Hit
TACGCAGGGATTCTGAAATCCTTTAACAATTCTGATGTGTTTTATTATTTTA1310.10510691218357604No Hit
TCGAGGGGTAAAGGCAACCCTGGCCCACACCCGCATGCGCCCGGTGCAGCCT1300.10430456934248004No Hit
CTTTATGGGACAAGCGATAAGCACAGGCACCTGACTTTTAAGTTTTTGTTTG1290.10350222650138403No Hit
TGCATGGGAAGCCGTGCAGAGCGAGCACAGCGACGCCAGCCCCATGTCCATC1290.10350222650138403No Hit
CTGATCGGGGTCAGAAGTTAATATTATGTAAAAGAGAAAAACAACAGAGAAA1290.10350222650138403No Hit
TTTGCAGGGAGAACACAAAACAACCCAAAAACAAATAACAAAATAGCAGGAG1290.10350222650138403No Hit
CTGCTTGGGACTGAAAATAAAATGAGTGTTATTTCCCTTGAGGGAAATGTAA1270.10189754081919204No Hit
GCAGCTGGGTAGCTAATTTCTGGATGAAGATAGGTAAATGCCTAGAATTTAC1270.10189754081919204No Hit
GCAGCAGGGTACAGTCGGGAGGCCTTATGAATGCCAAGAGTGTGGACGAAAG1260.10109519797809603No Hit
GCAGCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1260.10109519797809603No Hit
AGTTGGGGGACTTGGTATGGTTAGTCTTTTAAATTTTTGTGATTCTAATAAG1260.10109519797809603No Hit
ATACAGGGGTCAGGAATTTACATGGAATTCTTTGGAAAGAGACTCGATTTCC1250.10029285513700004No Hit
GTAGTAGGGCAGTTTCAGACTCAGCTTTTCTTGCTTGTGATCAAGTCCTGGT1250.10029285513700004No Hit
GCAATAGGAGGTTCCTGGTGGGGAGGGCTGGTGGGTGGCACTCACTGTACTC1250.10029285513700004No Hit
TTTTTAGGGGAGATGCGCCACGGTTTCGGTAGCGACGGTATCTCTAGCCGGG1250.10029285513700004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)