FastQCFastQC Report
Sat 31 Dec 2022
SRR5848070.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848070.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85472
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGCCTGTATCTCGTATGC2300.2690939722950206No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCCTGTATCTCGTATGCCGTCT2240.2620741295394983No Hit
TTACTAGGGGATCCGCAAAATGGCGCCTCGTGGTACCGGGTTTCCCGGGAGG1030.12050730063646574No Hit
GGCCTGGGGAAAGTGGGGTGCAGAAGGCATGACCCACGCCACAGCGGGCTCT1010.11816735305129164No Hit
CCCAGCTCTGCATTCTTGGGCAAGGTGGAATTTATAGCCTCTCAATGAAGCG1000.11699737925870461No Hit
TTGCACGGGCCCTAAATATATATACCTGTATTTTCCTAACTAAGCCAGTTAG1000.11699737925870461No Hit
GTCGCGGGATGACCACATGACCTGCAGAGCCCCTGTTATAGAACTCATTTTT990.11582740546611757No Hit
AGATCAGGGCGATCTTGTATAGGGGCACATTTACGATGTGGAGAAGGACTGC980.11465743167353051No Hit
ACTATTGGGATCCTGACACCATTCTCTCTCCATTTACTTCTGGTGGTTACCC970.11348745788094347No Hit
TCTAATGGGGTAGTGCCCTGCTTCTAGGGCTCTGAATACGGGCTTAAAGTCA960.11231748408835641No Hit
ACAAACGGGGATTTAGCAAGTTCTAGAAGGTCTCCAAGACAATAATTACATT950.11114751029576939No Hit
TAAAAGGGGAGAAGAGCAGGGAATGCTTCTTTAAGTACTTATTAGGGAGCAA950.11114751029576939No Hit
ATGTATGGGGTAAATGGACTAAATGCTCCAATTAAAAGACACAGACTGGCAA950.11114751029576939No Hit
TAGGCTGGGATCGTTCTCTGAGATTGCTTCTTTTGGAAACTGCACACATTAT940.10997753650318232No Hit
GTTAGCGGGAACCTAAGTTTCTGTTTGACATATGACACTGCAACTTAAAAAT920.10763758891800823No Hit
TGAAGGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCAAG900.10529764133283413No Hit
GAACAAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA890.1041276675402471No Hit
TAGGCTGGGATATCCAATGAAAATATTCTTCAAGAATGAGAGGGAAATAAAG890.1041276675402471No Hit
GGCTTTGGGATACCTCAATGAAAATCCCTTGAGGGCCTTGTACCTAATAGCA890.1041276675402471No Hit
ACTATAGGGAACTGAGTTGTAATAGAGAAGTAATTTATTTGAAATTATTTTA880.10295769374766006No Hit
TTAGTAGGGAGATGCTGACGGCTTCACACACCCTGGCTGTGGACGCCAAGAA870.101787719955073No Hit
AACCTGGGGCTCTTGGGATCTGTTATTATAACCAGTCATTTGTGATCTTATA870.101787719955073No Hit
CATACTGGGGATGTAAATGTCTGAAATGAGACACATAGAACTCAGAAGATGG860.10061774616248596No Hit
GTCAAAGGGGAAAATGAACAGTTCCAGATGAACAATTTGCACTCATGGGTGT860.10061774616248596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)