FastQCFastQC Report
Thu 19 Jan 2023
SRR5848042.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848042.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138414
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCCTGTATCTCGTATGCCGTCT5000.3612351351741876No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGCCTGTATCTCGTATGC4160.30054763246492405No Hit
CACTGCGGGATAGCTAGTATGTGGCAGAATCAAGATTCAAACCCAGGCTGTC2240.16183334055803603No Hit
ACTTGGGAGCTTGAGGCAGGAGGATCGCTGAGCCCAGGAGTTTGAGGCTCCA2220.16038840001733928No Hit
TAGCCCAGGTTCCCAGGGGGAGGGAAAATGGAGAAAATATAAAAAGATGTGA2060.14882887569176528No Hit
CGAAAGGGGTGTTTGCATAAGGAATGTTAAAATTCTTTCTACTACAATTTAT2040.14738393515106854No Hit
AAACCTGGGCAAGTACATTGATACCTTTGTATATTTATTTTTGCAGGTGAAA2030.14666146488072015No Hit
GCTCGGGTCAGCTTTGTCAAAGATCAGATGGTTGTAGGTACGCATCCTCATT1960.1416041729882815No Hit
CCGCTAGGGTCTCGGCCGTCACCAACCGCAGTGATCTGGGGCCCCCTGCCAG1910.13799182163653964No Hit
GACAAGGGGAACACACCAAATGAAAATGTGCAGGAGGAAACTAGCCTCAGGG1880.13582441082549454No Hit
TTACGAGGGACATTATTCCCTATCAAGACATAGCCAATTAATTTCATTGTCT1870.13510194055514615No Hit
CATGTGGGGATTCTTTACATCAGAAAAATCATGAATGTCAGTTTTGCCAGTA1870.13510194055514615No Hit
CTAAAAGGGCCCTAGCCTCTCGCTCACAGTGAGTCTGCATGTCCTAGATTCT1850.1336570000144494No Hit
GTTCAGGGGACATCTTAAGATCCTCAGGCAACTTAAACATTGGTTACTTGCC1850.1336570000144494No Hit
AGAGGTGGGGTTATTGTGATAAAAACATAACAAAAGTTACCATAGTAACCAT1840.13293452974410103No Hit
CGATAAGGGATGAAAAGAGCATTAATGAAAATGCTTGTCATGAAAAGCTAGA1830.13221205947375267No Hit
TGCTTCGGGGCTGCTCACTCCCAAAGCCTGACAAGTTGATGCCACTGCTGTC1820.13148958920340428No Hit
GACTTAGGGGTCTAGAAATGCACTTGAGATCACACAGCTATGAAAATGTCTT1810.1307671189330559No Hit
GGTTCAGGGCTGGCCCTAAAGTGGACAGTATTTTACAGCCACTTCTTCCTTG1800.1300446486627075No Hit
TTAATGGGGGATGGTAACAAGCATGCATGACATAAAACATTCCTCTTCATTA1800.1300446486627075No Hit
AAAAATGGGCATAAATACCCTACGTACTGGCATATTTGAAACTCTTTTTCCA1790.12932217839235916No Hit
TGTTGCGGGGCTCATGCAATTTTGTGTGTGAATTGGTCAAAAAGTAGAACCC1790.12932217839235916No Hit
ATAAACGGGGGGGCCAGCCTGGAGGCCCAGACGTGGCGCAGCGACTCGGAGG1740.12570982704061728No Hit
ACCATCGGGAAGTTAGGACCTGATTGAAAGGACGTTGAGAGCAAGGCTCGTG1740.12570982704061728No Hit
GACATAGGGATAAAAGAAGTCTCAAAATTCAAAGAACTAATGTTATTAAGGT1720.12426488649992054No Hit
CGTAACGGGAAATTCAGAGCACTCCAGTCAAGCAGTCAGGTGGAGGTTGCTG1710.12354241622957216No Hit
CTTCTAGGGGCTCTCTTCCAAGTCAGTCTAATGAGATACAATGAGTCTCGGG1690.1220974756888754No Hit
TCTTTTGGGGGGAAAATGTATTTCAAAAGAAAGTACTTTTATAGGAATGTCT1670.12065253514817866No Hit
GCTGCTGGGAAAAGGCATTTTGAAGTTGTGACTACACAAACAGATATCTTTG1670.12065253514817866No Hit
TTTAGGGGGCTCTTCCTGTCTGTACCAGGGCGGCGCGTGGTCTACGCCGAGT1660.11993006487783027No Hit
CATTCTGGGTGGTCAATACACAGTAATTCAGATTTTTCAATTATTTCTTTGG1660.11993006487783027No Hit
TAATATGGGGGGACCTTCTCGGCTGTGCTGGTCACCTATGCAGCCAGCGCCC1660.11993006487783027No Hit
TATTCTGGGGGCCCCAAAGACTAGCATCGTGACTCACCGGCAGTGACTCGTT1640.11848512433713353No Hit
ATTCGCGGGAAGTTACAGGCACATATTTGTGCATTATTTTGCATCTGCTACG1640.11848512433713353No Hit
CTAAGAACGGGATCTGATTCTGTGTCCCACTTCTTACCATTTCTGCCTTCAT1630.11776265406678516No Hit
CGCGCTGGGGCGCTCTCTTGGGGCTGAGGGAGCACCATGCAGCTTCACCCCT1630.11776265406678516No Hit
TTACTGGGGACCCTGAAGAGACAAGTTTTAGAAGTATCCTTGCTTTGTGCAT1630.11776265406678516No Hit
AAGTCAGGGATATGCTCAGTTTTGTTTTGTTTTGGCAGTTGACAAGAAGTTA1620.11704018379643677No Hit
AGTCTGGGGGCAGACGGCGGAGAGCAGAGAGGGAGCGCGCCTTGGCTCGCTG1610.1163177135260884No Hit
GAACCGGGGAGCCCAAGAAAACATCCACAAAAATAAAATCCCTTTAATTGGG1600.11559524325574003No Hit
CTCAAAGGGTGATGGCAAGAAAAATGAAAGACACAGACAGTGAAGAAGAAAT1600.11559524325574003No Hit
TTTGCAGGGGAAGGAGAGAAGAGGAAAATCAAAACTAAAATGATAGAGTATC1590.11487277298539164No Hit
TATCATGGGGGTAGCCTGTAACTTTGAACCATATAAACTTTTGTCAATTCTA1580.11415030271504328No Hit
CCGCCAGGGACACATCAATTGGACAACTTAAGACAAAATGCACCGATTCCTT1580.11415030271504328No Hit
AGTCTAGGGTACTCTCTGCCAGTGCAAGAATGGCCACATCTGTTGCTCAGTG1580.11415030271504328No Hit
CTAGACTGGATTGACTTGTTTTCCTTGTGTCTTCAGTGTGGCTTTCTTCCTC1570.1134278324446949No Hit
GCTACAGGGCAATTAACCAGCCTATCTGAAATGACATAATTTCGCACGTATA1560.11270536217434654No Hit
AATGGTGGGGAGTAGGTGGCCGCTTTGCGCCATCCGGGGCCAAGAGGGGGTG1560.11270536217434654No Hit
ACGACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1560.11270536217434654No Hit
ATTATGGGGATCCTTTCTTGCCTCTTCCTGTCTTCTGGTGGCTGCTAGAAGT1550.11198289190399816No Hit
GTGTGCGGGGTAATTCAGGAAACTATTTTCCTGATTTTTAAATAACTTTTAG1550.11198289190399816No Hit
GGTCATGGGATAGAATAAAAAGATATACTAAATACATCCATGAAAGAGATGG1550.11198289190399816No Hit
TAACTAGGGATGTCTTGTCCTGTAACTTAGTTCACAAAAACCTTGCTCCTTC1540.11126042163364977No Hit
GACAAAGGGGTGCTTTGTGTCTGAATTTCTCAGTATAGACATTTTGTTTACT1540.11126042163364977No Hit
ATTAAAGGGGTTTATTGATTTTGTTAAGCAAAGTATTTTGTAGTAAAGATAC1530.1105379513633014No Hit
TACATTGGGATAGATGGCTTTTATTACATTAAGGTATGTCCCCTGTATGCCA1530.1105379513633014No Hit
TGGCAGGGAAGTGAAATTGAAAACAGAGTTTTTAAATTTGTCTGGAGGACAT1520.10981548109295301No Hit
TGCACTGGGACGTGGTCCGTAAAGTTTAACTTTCTGATCCTTAATAGGAGGA1520.10981548109295301No Hit
TTTTGAGGGACAGCATTAAGCAAATAGACCTAAGTATTTGCCTTTATGAAGC1520.10981548109295301No Hit
CGACTGGGAAGGAAAGTGAACCTTTAAAGTAAAGGCCTCATCTCCTTTATTG1510.10909301082260466No Hit
TATTATGGGGGAGAATTTTTGAGACTTGGGTTTTCTTAATGCCAGTGTGAAT1510.10909301082260466No Hit
TTCTGGGGGGTGCTCTTTGAACCCAAGTCTGTGTGTGACATTCATACTGGGA1510.10909301082260466No Hit
ACTACAGGGCAGTCTGGATTCTGTCAGGAAACAGATGATACACTCAAAAGGA1510.10909301082260466No Hit
GGCCATGGGATGGGAACATGCCTCTGCTCACAGCTGGTAGCCACAGATGTAA1510.10909301082260466No Hit
TACCATGGGTAAATGCCTTTTTACAGCTTAATCATTTTGGTCTTCTGTTTAG1510.10909301082260466No Hit
TAATATGGGGGGGAAGGCTGGGCCCCCGGGAGAAGGCTCGCGGCTCTACTTC1500.10837054055225627No Hit
GTACCGGGGGTTACCCACAGCAGAATCAAATGAGTCTGTTAAAAAAATTTTT1500.10837054055225627No Hit
CGAATCGGGGTTTCATGCGGTGGCCTCTGCTGGCTCTAGTCTTGTCTTCCTT1500.10837054055225627No Hit
GATACTGGGGCAGTCCTTCTTTGCACCTTATTTTAAAGAATAAATGAAACAT1500.10837054055225627No Hit
GGTGCCGGGCAACCTCACAGCAGAGGAGATCAAGCGGGAGCAGAGGCTCAGG1490.10764807028190791No Hit
TGCTGTGGGGCGATCTTAGACGGGCTGTATTTGCAAATCCAACATTGAGCTT1490.10764807028190791No Hit
TATACTGGGACTTGAAGAGTTTATACTCAAACGGCGGTGTAGCCAGACTAGG1490.10764807028190791No Hit
GCAATAGGGATTTCAACTTTGTGTTATAGTGAAGTATTTAATATGACACTTT1490.10764807028190791No Hit
GTCAATGGGCAGGGAGGGTGTTTTGGGTGGCATAGAGGTTTTGTATTGAGGG1480.10692560001155953No Hit
AGCCTGGGGGGGAGAGACTGGTTATCTCCTCCCATATGGTTGTGCTTATCCT1470.10620312974121114No Hit
AACTCAGGGATGGGAACTGACATTTCAGAAAAGGATCCTAAAGGAATTTTTA1470.10620312974121114No Hit
GCAATAGGGGGTTGAGTTAGAATAGCAGACATCCATCGAGACTTTCCAGGGC1470.10620312974121114No Hit
TCATGTGGGGGAACAAACAAAGCCTCCAAGAATTATGGGACTATGTGAAAAG1460.10548065947086277No Hit
GAACACGGGATCGGCATCTGAGCCAGCTGGTAGAGGGCATTACCTCCCCCAC1460.10548065947086277No Hit
TGGCTAAGGGGGATGGAAGATGTTACATCTGATTTTGAAAAATATACTTCTC1460.10548065947086277No Hit
GTAATGGGGATGAAAATGCTAGTTCCTTTAAGCACTTACTGACTGTAAAGTT1460.10548065947086277No Hit
CTACCAGGGATGTTTTTAGATGAACTTGGGAATGTACCTGAACTTCCGGACA1460.10548065947086277No Hit
TACAGGGGACAGACAGGCCCTGAATGAGCACTTCCAGTCCATTCTGCAGACT1450.10475818920051438No Hit
TTGCTTGGGCCAGCGTCATGCGCTTCTGCTGCTGCGTGGCTGTCATCTACCT1450.10475818920051438No Hit
GTAATTGGGGCACACATGCAGACTTTGCTCATCCCTCTTGGCTGACAAACTA1450.10475818920051438No Hit
AGCACTGGATTCTAAAGAAAAAAATGGGATTTGTTTTCTCGGCAGATCTGCA1450.10475818920051438No Hit
CTGCCTCGGGGTATTTGGCAAAGGCACTTGAGAGCAAAATCTTCCTTAGCAA1450.10475818920051438No Hit
GAAATTGGGAAGGCGCTTTGGAAAGGCGAAGCCTGGGCGCAGGGAGCCCAGC1450.10475818920051438No Hit
TTAATGGGGTCCGATCTTATCCGGGGGGCGCCGTGTGGCACTCGGCGGTCGA1440.10403571893016603No Hit
CCGCATGGGATGAGAGGAAGATGATACCTATCTGGAAACAACAGGCGAGACC1440.10403571893016603No Hit
CTTTATGGGTGGGGAGGAGGGAAGGTGCTGATACCTTCAATTTGTTACTTTT1440.10403571893016603No Hit
ATACAAGGGGTCTCCTCCATCTGTGATTTTGTTTATTTAGTCTCTCTGTTAT1430.10331324865981764No Hit
CTATAAGGGAAGCAGAAAACAGTGCCCCAAATGGAAAAAAGATACTCACACA1430.10331324865981764No Hit
TCTGTGGGGACATTCAACTTATATTGAATATCTTAGTGTACCATTCTAAATT1430.10331324865981764No Hit
CAGTCGGGGACCGCCGAGACCGCGTCCGCCCCGCGAGCACAGAGCCTCGCCT1430.10331324865981764No Hit
CTATTGGGGTGACCTCCAGAGTGGGCACAATCAGGGTCCAACTTTGTTTTGT1430.10331324865981764No Hit
CGTTGCGGGCTTCAATGATAAATTAACCTTAGCTTACTGTAATGGCTTGTTT1420.10259077838946928No Hit
GTGACAGGGGTGTTGTTCAAGACCCTGTTCAAGTCATTTGGACAAATTTAAT1420.10259077838946928No Hit
ACTGCTGGGACCTGGGCAACGTCTGCATCAACTGCCGCCAGCCCTTCATCTT1420.10259077838946928No Hit
GATTCAGGGATTACCTACGAATCCATGAAGTAAAGGTAGCAACTGTTAAGCT1410.1018683081191209No Hit
CAACCCGGGTGCCAGTGGTTCTAGTGGGGAACAAGGCAGATCTCTCTCCAGA1410.1018683081191209No Hit
TAAGTCGGGGGAATGATACTGTCTAGACATTTCTAATATGTTAGACTTGATG1410.1018683081191209No Hit
TGCTATCGGGTGACCTCACATCATATTTGGTTCTCCTACTGACCTTTGATCT1410.1018683081191209No Hit
GCAGTAGGGATGCCCAGGTCTAGGCTGAGAAGTCCTTTTTCTTCTTCCCACC1400.10114583784877254No Hit
CGAACTGGGGGAAAGGCCACAGTTATGTTTCCTTTGAATGGAAGAGAGACAA1400.10114583784877254No Hit
CGTTGGCGGGGTTCATGCTGCGGTTGAGGCGCCTTGGTACCCTGTAGTGGTC1390.10042336757842416No Hit
ATTCAGGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1390.10042336757842416No Hit
CTTACTGGGTCTTCACAGGGGACAGCTGACATATAGTGTATTACCTTTGCAG1390.10042336757842416No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCG206.270232E-446.032541
CATATTG206.270232E-446.032541
TTCAGCG206.270232E-446.032541
GTTTACG206.270232E-446.032541
CAGTTCG206.270232E-446.032541
AACCACG206.270232E-446.032541
ATTCGCG405.515176E-946.032541
CCCGTGG206.270232E-446.032541
AGTGCCG206.270232E-446.032541
TATTCCG206.270232E-446.032541
GTCGTAG351.0052645E-746.032541
GGTGCCG351.0052645E-746.032541
TATACCG206.270232E-446.032541
CTAATGG206.270232E-446.032541
ATACACG206.270232E-446.032541
ATACAAG453.0377123E-1046.032541
TCGCCTG253.387302E-546.0325361
CGAACTG253.387302E-546.0325361
TGCTTCG253.387302E-546.0325361
CACATCG253.387302E-546.0325361
Produced by FastQC (version 0.11.5)