FastQCFastQC Report
Sat 31 Dec 2022
SRR5848041.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848041.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139420
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCCTGTATCTCGTATGCCGTCT5320.38158083488739064No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGCCTGTATCTCGTATGC4540.32563477262946494No Hit
ACTTGGGAGCTTGAGGCAGGAGGATCGCTGAGCCCAGGAGTTTGAGGCTCCA2550.1829005881509109No Hit
GCTCGGGTCAGCTTTGTCAAAGATCAGATGGTTGTAGGTACGCATCCTCATT2330.16712092956534214No Hit
CTAAAAGGGCCCTAGCCTCTCGCTCACAGTGAGTCTGCATGTCCTAGATTCT2270.16281738631473247No Hit
TAGCCCAGGTTCCCAGGGGGAGGGAAAATGGAGAAAATATAAAAAGATGTGA2240.1606656146894276No Hit
CGAAAGGGGTGTTTGCATAAGGAATGTTAAAATTCTTTCTACTACAATTTAT2200.15779658585568784No Hit
GACAAGGGGAACACACCAAATGAAAATGTGCAGGAGGAAACTAGCCTCAGGG2170.155644814230383No Hit
CCGCTAGGGTCTCGGCCGTCACCAACCGCAGTGATCTGGGGCCCCCTGCCAG2090.14990675656290345No Hit
TTACGAGGGACATTATTCCCTATCAAGACATAGCCAATTAATTTCATTGTCT2050.14703772772916368No Hit
TAATATGGGGGGACCTTCTCGGCTGTGCTGGTCACCTATGCAGCCAGCGCCC2000.14345144168698895No Hit
CACTGCGGGATAGCTAGTATGTGGCAGAATCAAGATTCAAACCCAGGCTGTC1970.14129967006168412No Hit
CGACTGGGAAGGAAAGTGAACCTTTAAAGTAAAGGCCTCATCTCCTTTATTG1960.14058241285324916No Hit
GTTCAGGGGACATCTTAAGATCCTCAGGCAACTTAAACATTGGTTACTTGCC1890.13556161239420456No Hit
TATTCTGGGGGCCCCAAAGACTAGCATCGTGACTCACCGGCAGTGACTCGTT1880.13484435518576962No Hit
CAACCCGGGTGCCAGTGGTTCTAGTGGGGAACAAGGCAGATCTCTCTCCAGA1850.1326925835604648No Hit
AAGTCAGGGATATGCTCAGTTTTGTTTTGTTTTGGCAGTTGACAAGAAGTTA1840.13197532635202983No Hit
TAACTAGGGATGTCTTGTCCTGTAACTTAGTTCACAAAAACCTTGCTCCTTC1840.13197532635202983No Hit
TTGCTTGGGCCAGCGTCATGCGCTTCTGCTGCTGCGTGGCTGTCATCTACCT1830.1312580691435949No Hit
CAGTCGGGGACCGCCGAGACCGCGTCCGCCCCGCGAGCACAGAGCCTCGCCT1830.1312580691435949No Hit
TACCATGGGTAAATGCCTTTTTACAGCTTAATCATTTTGGTCTTCTGTTTAG1780.12767178310142016No Hit
TGCTTCGGGGCTGCTCACTCCCAAAGCCTGACAAGTTGATGCCACTGCTGTC1770.12695452589298523No Hit
TTTAGGGGGCTCTTCCTGTCTGTACCAGGGCGGCGCGTGGTCTACGCCGAGT1750.12552001147611536No Hit
GATACTGGGGCAGTCCTTCTTTGCACCTTATTTTAAAGAATAAATGAAACAT1750.12552001147611536No Hit
CTAAGAACGGGATCTGATTCTGTGTCCCACTTCTTACCATTTCTGCCTTCAT1730.12408549705924544No Hit
TGTTGCGGGGCTCATGCAATTTTGTGTGTGAATTGGTCAAAAAGTAGAACCC1710.12265098264237556No Hit
GAACCGGGGAGCCCAAGAAAACATCCACAAAAATAAAATCCCTTTAATTGGG1700.12193372543394061No Hit
ACTACAGGGCAGTCTGGATTCTGTCAGGAAACAGATGATACACTCAAAAGGA1700.12193372543394061No Hit
GAACACGGGATCGGCATCTGAGCCAGCTGGTAGAGGGCATTACCTCCCCCAC1690.12121646822550566No Hit
CTTCTAGGGGCTCTCTTCCAAGTCAGTCTAATGAGATACAATGAGTCTCGGG1690.12121646822550566No Hit
TTGCATGCGGGGCGCCCGAAGCCGTCGCGGCGGGGACCATGTTGCTTCCGAA1690.12121646822550566No Hit
TGCTATCGGGTGACCTCACATCATATTTGGTTCTCCTACTGACCTTTGATCT1690.12121646822550566No Hit
GAAATTGGGAAGGCGCTTTGGAAAGGCGAAGCCTGGGCGCAGGGAGCCCAGC1690.12121646822550566No Hit
TCATGTGGGGGAACAAACAAAGCCTCCAAGAATTATGGGACTATGTGAAAAG1680.12049921101707071No Hit
TCAGTCGGGGTCAATTAAAGCTATATGTCTAAAATTCGTAGGGCAACTTCTG1680.12049921101707071No Hit
AACTCAGGGATGGGAACTGACATTTCAGAAAAGGATCCTAAAGGAATTTTTA1680.12049921101707071No Hit
GCAACGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGG1680.12049921101707071No Hit
CAGTTCGGGATGGTAGAAATCTTGGTTTTGGCACTTAATGGTATAGTAACCA1670.11978195380863577No Hit
CTCAAAGGGTGATGGCAAGAAAAATGAAAGACACAGACAGTGAAGAAGAAAT1670.11978195380863577No Hit
TTTGCAGGGGAAGGAGAGAAGAGGAAAATCAAAACTAAAATGATAGAGTATC1670.11978195380863577No Hit
ATTCGCGGGAAGTTACAGGCACATATTTGTGCATTATTTTGCATCTGCTACG1670.11978195380863577No Hit
GATTCAGGGATTACCTACGAATCCATGAAGTAAAGGTAGCAACTGTTAAGCT1660.11906469660020083No Hit
AATGGTGGGGAGTAGGTGGCCGCTTTGCGCCATCCGGGGCCAAGAGGGGGTG1660.11906469660020083No Hit
ATACAAGGGGTCTCCTCCATCTGTGATTTTGTTTATTTAGTCTCTCTGTTAT1660.11906469660020083No Hit
GACATAGGGATAAAAGAAGTCTCAAAATTCAAAGAACTAATGTTATTAAGGT1660.11906469660020083No Hit
CGAACTGGGGGAAAGGCCACAGTTATGTTTCCTTTGAATGGAAGAGAGACAA1660.11906469660020083No Hit
GTCGTAGGGGATTTAACCCTCACCTGGTGCAAATGCATCATGTAAATTTTGA1650.11834743939176588No Hit
CGCGCTGGGGCGCTCTCTTGGGGCTGAGGGAGCACCATGCAGCTTCACCCCT1650.11834743939176588No Hit
TTACTGGGGACCCTGAAGAGACAAGTTTTAGAAGTATCCTTGCTTTGTGCAT1630.11691292497489598No Hit
CTACCAGGGATGTTTTTAGATGAACTTGGGAATGTACCTGAACTTCCGGACA1620.11619566776646106No Hit
CGAATCGGGGTTTCATGCGGTGGCCTCTGCTGGCTCTAGTCTTGTCTTCCTT1610.11547841055802611No Hit
AAATGGGGATTAATTCAGGTTACAGTCACGGCAGTCGCAATCACTTTTGACA1610.11547841055802611No Hit
CCATGTTCCGTAATGTGCCCACAGCAAACCCCACGGAGCCGGGAATCAGACG1610.11547841055802611No Hit
GTTAATGGGATCCTGCAAATTTCCATCTGCATTTTATTGCCCAGAGATTTCA1610.11547841055802611No Hit
CGATAAGGGATGAAAAGAGCATTAATGAAAATGCTTGTCATGAAAAGCTAGA1600.11476115334959117No Hit
CATTCTGGGTGGTCAATACACAGTAATTCAGATTTTTCAATTATTTCTTTGG1600.11476115334959117No Hit
CTTTATGGGTGGGGAGGAGGGAAGGTGCTGATACCTTCAATTTGTTACTTTT1600.11476115334959117No Hit
GTGTGCGGGGTAATTCAGGAAACTATTTTCCTGATTTTTAAATAACTTTTAG1590.11404389614115623No Hit
TGCATAGGGTGAAAAACACAGTACAAGAACTTCGTGAAGCATACACAAGTAT1590.11404389614115623No Hit
GCTGCTGGGAAAAGGCATTTTGAAGTTGTGACTACACAAACAGATATCTTTG1590.11404389614115623No Hit
CATGTGGGGATTCTTTACATCAGAAAAATCATGAATGTCAGTTTTGCCAGTA1580.11332663893272128No Hit
GTCAATGGGCAGGGAGGGTGTTTTGGGTGGCATAGAGGTTTTGTATTGAGGG1580.11332663893272128No Hit
GGTGCCGGGCAACCTCACAGCAGAGGAGATCAAGCGGGAGCAGAGGCTCAGG1570.11260938172428633No Hit
TCATGCGGGGTGCTTCTAGCAGCTGTTCGGGAGGACCTTGACCCAATAGTGT1570.11260938172428633No Hit
AAGGTTGGGCCCTGCTGAGCAGCGTGTTTGGTGCGTGTGGCCTGCTCCTGGT1570.11260938172428633No Hit
GCAAAAGGGAAAAAAAAGAAGCGGGAGAATCTGGGCGTCGCTCTAGAGATCG1570.11260938172428633No Hit
GTAATTGGGGCACACATGCAGACTTTGCTCATCCCTCTTGGCTGACAAACTA1560.11189212451585138No Hit
TTTCATGGGAGAAATGGAAATGCCTGGCACAGCCAGGAAGGAGCTGCCCATG1560.11189212451585138No Hit
TATCATGGGGGTAGCCTGTAACTTTGAACCATATAAACTTTTGTCAATTCTA1560.11189212451585138No Hit
TTGATAGGGAGAATCAGAAGACCTACTGCTGTTTGCACCTTAAAAAACTAAA1560.11189212451585138No Hit
TACAGGGGACAGACAGGCCCTGAATGAGCACTTCCAGTCCATTCTGCAGACT1550.11117486730741644No Hit
AGTCTGGGGGCAGACGGCGGAGAGCAGAGAGGGAGCGCGCCTTGGCTCGCTG1550.11117486730741644No Hit
CCGCATGGGATGAGAGGAAGATGATACCTATCTGGAAACAACAGGCGAGACC1540.1104576100989815No Hit
GACTTAGGGGTCTAGAAATGCACTTGAGATCACACAGCTATGAAAATGTCTT1540.1104576100989815No Hit
ACACAAATCAAGAAAAAAGGAGAATACTCAATTTACTAAAATAAAAATGAAA1540.1104576100989815No Hit
TTAACAGGGTGGCATTAGTGCTCAGACCCCTGAGGAGGCTTACCACCTACCT1540.1104576100989815No Hit
GACAAAGGGGTGCTTTGTGTCTGAATTTCTCAGTATAGACATTTTGTTTACT1540.1104576100989815No Hit
GGCCATGGGATGGGAACATGCCTCTGCTCACAGCTGGTAGCCACAGATGTAA1540.1104576100989815No Hit
AGAGGTGGGGTTATTGTGATAAAAACATAACAAAAGTTACCATAGTAACCAT1530.10974035289054655No Hit
AAATCCGGGCTACAGAAAATCCAAAGAGGAAATCTACTAGATGCTTTGAGAT1520.1090230956821116No Hit
CGTTGCGGGCTTCAATGATAAATTAACCTTAGCTTACTGTAATGGCTTGTTT1520.1090230956821116No Hit
ACGCCAGGGAACTTTAGAGGGATTGAAATCTGTTGCCTGTTCCACTAGGAAT1520.1090230956821116No Hit
AAACCTGGGCAAGTACATTGATACCTTTGTATATTTATTTTTGCAGGTGAAA1520.1090230956821116No Hit
TTTGCAGGGATCCTGTTTAATTCTTCAGTTTAAATTTACCAACAAACATAAA1510.10830583847367665No Hit
TGGCTAAGGGGGATGGAAGATGTTACATCTGATTTTGAAAAATATACTTCTC1510.10830583847367665No Hit
TATCCTTGGGGAGAACTTTTTTTTAGTTTTTACCTTTTCTTAATTACCCTTA1510.10830583847367665No Hit
CCCATCCCATCTATCTTGTAAACCAAACAACCAAACTGCATCAGTCGGCTAA1510.10830583847367665No Hit
TAAGTCGGGGGAATGATACTGTCTAGACATTTCTAATATGTTAGACTTGATG1510.10830583847367665No Hit
AAAAATGGGCATAAATACCCTACGTACTGGCATATTTGAAACTCTTTTTCCA1510.10830583847367665No Hit
ATTATGGGGATCCTTTCTTGCCTCTTCCTGTCTTCTGGTGGCTGCTAGAAGT1500.1075885812652417No Hit
TTTTGTGGGCTAGACTTGCCCGTTTATCTCAAAAGTTATTCTTCAATCTGAT1500.1075885812652417No Hit
ATAGGGGATTTTGTTCAACAAATATTTATTACATGCCTCCTCTACATCAGGC1500.1075885812652417No Hit
TCTTTTGGGGGGAAAATGTATTTCAAAAGAAAGTACTTTTATAGGAATGTCT1500.1075885812652417No Hit
GAATAGGGGATGTCATGTAAAAGTGGAATGCATGCTTGTGTATTCTTCTGTT1500.1075885812652417No Hit
TATTATGGGGGAGAATTTTTGAGACTTGGGTTTTCTTAATGCCAGTGTGAAT1490.10687132405680676No Hit
ATTAAAGGGGTTTATTGATTTTGTTAAGCAAAGTATTTTGTAGTAAAGATAC1490.10687132405680676No Hit
TGCACGGGAACACTTAAGCTATCCATGGACAGCAAAGCTAAAAGGGCACTGT1490.10687132405680676No Hit
ACGACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1490.10687132405680676No Hit
ATGCGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1490.10687132405680676No Hit
AGCCTGGGGGGGAGAGACTGGTTATCTCCTCCCATATGGTTGTGCTTATCCT1480.10615406684837182No Hit
TTAATGGGGGATGGTAACAAGCATGCATGACATAAAACATTCCTCTTCATTA1480.10615406684837182No Hit
TTTTGAGGGACAGCATTAAGCAAATAGACCTAAGTATTTGCCTTTATGAAGC1480.10615406684837182No Hit
CTTAAAGGGAGACTTTCCGGGCAAGGGAGAACTGGGAACTTTCATCTTAAAA1470.10543680963993687No Hit
ATTCAGGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1470.10543680963993687No Hit
GCACAGCGGGCTGACACCAAAATGCATTTCACCATTGGCTGCTGTCGGTAGA1470.10543680963993687No Hit
TTTGCTGGGCTAACGCAGGGGTTTCTAAGTGAGCTCCCTGAGGTGCCAAGGC1460.10471955243150195No Hit
TTAGTAGGGAAGTTAACAAACATATAAGAAATGTATGCACTGTTTGAAATGT1460.10471955243150195No Hit
TATATGGGGACATTTATTGATACCTACTGTGTGCTGGGTGTTACAGCTACTA1460.10471955243150195No Hit
CACATCGGGGTTGTGTTTTAAATGCTAAAAGACATGCTGTGTTTTAAAATTT1460.10471955243150195No Hit
AGGTTCGGGTTAGTTAGATTCTCATAATGAGCGCAACTTAGACCCCTCACAT1460.10471955243150195No Hit
CCGCCAGGGACACATCAATTGGACAACTTAAGACAAAATGCACCGATTCCTT1460.10471955243150195No Hit
CACCTGGGGCATCTCAGATCCCGGTTCCTCTCGGCGAATGCTGCTTGCGAAT1450.104002295223067No Hit
TTGAATGGGGTATTTTGGCAAAGGCTTAAAAGGATCATTGAAAGTCCTGACT1450.104002295223067No Hit
TCAGCCCGTACCAAAAAGTAGAGTGGAGCCTCTTTGCACTACTACTATCAAT1440.10328503801463206No Hit
TTGCAGTGGGGTATGTGAATAAAATACGTATGTTAAATCAAAGATTTAGTAA1440.10328503801463206No Hit
AGTCTAGGGTACTCTCTGCCAGTGCAAGAATGGCCACATCTGTTGCTCAGTG1440.10328503801463206No Hit
GTACCGGGGGTTACCCACAGCAGAATCAAATGAGTCTGTTAAAAAAATTTTT1430.1025677808061971No Hit
TAATATGGGAGCTGTATTGTCATATATCAAGTACTGAGTGAAGTACCTAAAA1430.1025677808061971No Hit
GCATTTGGGGTCCCTATCTCTGACTGTGCAGTGAGTGTGGCCTAGGAACAGG1430.1025677808061971No Hit
ATAAACGGGGGGGCCAGCCTGGAGGCCCAGACGTGGCGCAGCGACTCGGAGG1430.1025677808061971No Hit
GGTATTGGGATTAATATCAAATTCACTTATGAATAAACTTGATAATGGAAAC1430.1025677808061971No Hit
TTTTAGGGGGGAGTGTAGCTACTTTAACAGATGGAAAGAACCTCATTGGCCA1430.1025677808061971No Hit
TTTTATGGGCCACTGCGCCCAGCCAGATTTCTCAATTCTTTAAACACACACA1430.1025677808061971No Hit
TTGAATGGGACCTTAGTCCTGGGCCAACCAGACGGGCTGGTAGTACCAAAAG1430.1025677808061971No Hit
GCAGTAGGGATGCCCAGGTCTAGGCTGAGAAGTCCTTTTTCTTCTTCCCACC1420.10185052359776216No Hit
TGCTGTGGGGCGATCTTAGACGGGCTGTATTTGCAAATCCAACATTGAGCTT1420.10185052359776216No Hit
CTATAAGGGAAGCAGAAAACAGTGCCCCAAATGGAAAAAAGATACTCACACA1420.10185052359776216No Hit
GAGAAAGGGAGGCACATTTCACTGGCATTTTCAATATTCTTTACTTGAGAAG1410.10113326638932721No Hit
AACTGCGGGATGGCAGTGCCAAGGGAGCTGTGCAAGTATAATCAAAGCTGGA1410.10113326638932721No Hit
AGCACTGGATTCTAAAGAAAAAAATGGGATTTGTTTTCTCGGCAGATCTGCA1410.10113326638932721No Hit
AAAAATGGGGTTTTTACTTTTAATAAAAACCAGTATATCTTAGCTATTGCTT1400.10041600918089227No Hit
CTGCCTCGGGGTATTTGGCAAAGGCACTTGAGAGCAAAATCTTCCTTAGCAA1400.10041600918089227No Hit
CATTATTGGGAATGAATAGATGAGTGAAAGGAGTTTTCTTTTTCTCTTTAGT1400.10041600918089227No Hit
TTTTGAGGGTGTGGCTATTGTAAATAAGACTGCATTCTTTATTTAATTCTCA1400.10041600918089227No Hit
TACATTGGGATAGATGGCTTTTATTACATTAAGGTATGTCCCCTGTATGCCA1400.10041600918089227No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)