FastQCFastQC Report
Sat 31 Dec 2022
SRR5848040.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848040.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137168
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCCTGTATCTCGTATGCCGTCT5070.36961973638166334No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGCCTGTATCTCGTATGC4380.3193164586492476No Hit
TAATATGGGGGGACCTTCTCGGCTGTGCTGGTCACCTATGCAGCCAGCGCCC2200.16038726233523853No Hit
TAGCCCAGGTTCCCAGGGGGAGGGAAAATGGAGAAAATATAAAAAGATGTGA2180.1589291963140091No Hit
AAACCTGGGCAAGTACATTGATACCTTTGTATATTTATTTTTGCAGGTGAAA2120.15455499825032076No Hit
GACAAGGGGAACACACCAAATGAAAATGTGCAGGAGGAAACTAGCCTCAGGG2000.14580660212294413No Hit
TGCTTCGGGGCTGCTCACTCCCAAAGCCTGACAAGTTGATGCCACTGCTGTC1970.14361950309109997No Hit
CACTGCGGGATAGCTAGTATGTGGCAGAATCAAGATTCAAACCCAGGCTGTC1960.14289047008048525No Hit
TTGCTTGGGCCAGCGTCATGCGCTTCTGCTGCTGCGTGGCTGTCATCTACCT1960.14289047008048525No Hit
GCTCGGGTCAGCTTTGTCAAAGATCAGATGGTTGTAGGTACGCATCCTCATT1960.14289047008048525No Hit
TTTTATGGGCCACTGCGCCCAGCCAGATTTCTCAATTCTTTAAACACACACA1880.13705820599556748No Hit
CTCAAAGGGTGATGGCAAGAAAAATGAAAGACACAGACAGTGAAGAAGAAAT1850.13487110696372331No Hit
GCAACGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGG1840.1341420739531086No Hit
CATGTGGGGATTCTTTACATCAGAAAAATCATGAATGTCAGTTTTGCCAGTA1840.1341420739531086No Hit
AATGGTGGGGAGTAGGTGGCCGCTTTGCGCCATCCGGGGCCAAGAGGGGGTG1820.13268400793187915No Hit
AGTCTAGGGTACTCTCTGCCAGTGCAAGAATGGCCACATCTGTTGCTCAGTG1810.13195497492126443No Hit
CTAAAAGGGCCCTAGCCTCTCGCTCACAGTGAGTCTGCATGTCCTAGATTCT1800.1312259419106497No Hit
TGTTGCGGGGCTCATGCAATTTTGTGTGTGAATTGGTCAAAAAGTAGAACCC1800.1312259419106497No Hit
GTTCAGGGGACATCTTAAGATCCTCAGGCAACTTAAACATTGGTTACTTGCC1780.12976787588942026No Hit
GATACTGGGGCAGTCCTTCTTTGCACCTTATTTTAAAGAATAAATGAAACAT1780.12976787588942026No Hit
GGCCATGGGATGGGAACATGCCTCTGCTCACAGCTGGTAGCCACAGATGTAA1780.12976787588942026No Hit
ACTTGGGAGCTTGAGGCAGGAGGATCGCTGAGCCCAGGAGTTTGAGGCTCCA1750.1275807768575761No Hit
GACTTAGGGGTCTAGAAATGCACTTGAGATCACACAGCTATGAAAATGTCTT1740.1268517438469614No Hit
CGACTGGGAAGGAAAGTGAACCTTTAAAGTAAAGGCCTCATCTCCTTTATTG1740.1268517438469614No Hit
TCATGTGGGGGAACAAACAAAGCCTCCAAGAATTATGGGACTATGTGAAAAG1710.12466464481511723No Hit
GAACACGGGATCGGCATCTGAGCCAGCTGGTAGAGGGCATTACCTCCCCCAC1710.12466464481511723No Hit
CGAAAGGGGTGTTTGCATAAGGAATGTTAAAATTCTTTCTACTACAATTTAT1710.12466464481511723No Hit
AAAAATGGGCATAAATACCCTACGTACTGGCATATTTGAAACTCTTTTTCCA1700.1239356118045025No Hit
AAATCCGGGCTACAGAAAATCCAAAGAGGAAATCTACTAGATGCTTTGAGAT1690.12320657879388779No Hit
TATTCTGGGGGCCCCAAAGACTAGCATCGTGACTCACCGGCAGTGACTCGTT1680.12247754578327306No Hit
CAGTTCGGGATGGTAGAAATCTTGGTTTTGGCACTTAATGGTATAGTAACCA1670.12174851277265833No Hit
TATTATGGGGGAGAATTTTTGAGACTTGGGTTTTCTTAATGCCAGTGTGAAT1670.12174851277265833No Hit
ACGCCAGGGAACTTTAGAGGGATTGAAATCTGTTGCCTGTTCCACTAGGAAT1660.12101947976204362No Hit
ACCATCGGGAAGTTAGGACCTGATTGAAAGGACGTTGAGAGCAAGGCTCGTG1660.12101947976204362No Hit
CCGCTAGGGTCTCGGCCGTCACCAACCGCAGTGATCTGGGGCCCCCTGCCAG1650.12029044675142891No Hit
TAAGTCGGGGGAATGATACTGTCTAGACATTTCTAATATGTTAGACTTGATG1640.11956141374081419No Hit
AGCCTGGGGGGGAGAGACTGGTTATCTCCTCCCATATGGTTGTGCTTATCCT1630.11883238073019946No Hit
TCTTTTGGGGGGAAAATGTATTTCAAAAGAAAGTACTTTTATAGGAATGTCT1630.11883238073019946No Hit
TACATTGGGATAGATGGCTTTTATTACATTAAGGTATGTCCCCTGTATGCCA1630.11883238073019946No Hit
CCATGTTCCGTAATGTGCCCACAGCAAACCCCACGGAGCCGGGAATCAGACG1620.11810334771958475No Hit
TACCATGGGTAAATGCCTTTTTACAGCTTAATCATTTTGGTCTTCTGTTTAG1620.11810334771958475No Hit
TTTGCAGGGGAAGGAGAGAAGAGGAAAATCAAAACTAAAATGATAGAGTATC1610.11737431470897003No Hit
GAACCGGGGAGCCCAAGAAAACATCCACAAAAATAAAATCCCTTTAATTGGG1600.1166452816983553No Hit
GACATAGGGATAAAAGAAGTCTCAAAATTCAAAGAACTAATGTTATTAAGGT1600.1166452816983553No Hit
GTATAGGGGCTTTATAAACTTGACTTGCTATCTCAGCAAGATAAATTATATT1580.11518721567712586No Hit
CCGCCAGGGACACATCAATTGGACAACTTAAGACAAAATGCACCGATTCCTT1580.11518721567712586No Hit
ATTCAGGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1570.11445818266651113No Hit
CCAGAGGGTGGCCTCATAAAGGGGTTCCACTGTACTTTCCGCATATTACTGT1570.11445818266651113No Hit
ACCAGGGATCAAAATATAATGTTAATATGATTTGTCACTGAACTATATGATA1560.11372914965589642No Hit
CCGCATGGGATGAGAGGAAGATGATACCTATCTGGAAACAACAGGCGAGACC1560.11372914965589642No Hit
TGCACGGGAACACTTAAGCTATCCATGGACAGCAAAGCTAAAAGGGCACTGT1560.11372914965589642No Hit
CCACCTCCCTGCACTTGTAGTCTAAAAAGTAAAAGGGGCGATCAGGTCCATC1560.11372914965589642No Hit
TCGTAGGGGTATTCAGGTATGGCTACGTAATCTATGGTACAAAATGTAAGTA1560.11372914965589642No Hit
TTTGCAGGGATCCTGTTTAATTCTTCAGTTTAAATTTACCAACAAACATAAA1550.1130001166452817No Hit
ATACAAGGGGTCTCCTCCATCTGTGATTTTGTTTATTTAGTCTCTCTGTTAT1550.1130001166452817No Hit
TATCATGGGGGTAGCCTGTAACTTTGAACCATATAAACTTTTGTCAATTCTA1550.1130001166452817No Hit
GATTCAGGGATTACCTACGAATCCATGAAGTAAAGGTAGCAACTGTTAAGCT1530.11154205062405226No Hit
AAAAATGGGGTTTTTACTTTTAATAAAAACCAGTATATCTTAGCTATTGCTT1530.11154205062405226No Hit
GTGTGCGGGGTAATTCAGGAAACTATTTTCCTGATTTTTAAATAACTTTTAG1530.11154205062405226No Hit
TGCTATCGGGTGACCTCACATCATATTTGGTTCTCCTACTGACCTTTGATCT1530.11154205062405226No Hit
TCAGTCGGGGTCAATTAAAGCTATATGTCTAAAATTCGTAGGGCAACTTCTG1520.11081301761343755No Hit
AAATGGGGATTAATTCAGGTTACAGTCACGGCAGTCGCAATCACTTTTGACA1520.11081301761343755No Hit
GCTCATGGGACTGTGACAGGTTTTGCCCAAGGCTTTGATACCTGTCACCCAG1520.11081301761343755No Hit
TGCACTGGGACGTGGTCCGTAAAGTTTAACTTTCTGATCCTTAATAGGAGGA1520.11081301761343755No Hit
TTTGCGCGGGATGAGATATCATCTCACACCAGTTAGAATGGCAGTCATTAAA1520.11081301761343755No Hit
CTACCAGGGATGTTTTTAGATGAACTTGGGAATGTACCTGAACTTCCGGACA1520.11081301761343755No Hit
CGTTGCGGGCTTCAATGATAAATTAACCTTAGCTTACTGTAATGGCTTGTTT1510.11008398460282282No Hit
TTAATGGGGGATGGTAACAAGCATGCATGACATAAAACATTCCTCTTCATTA1510.11008398460282282No Hit
TAACTAGGGATGTCTTGTCCTGTAACTTAGTTCACAAAAACCTTGCTCCTTC1510.11008398460282282No Hit
TATATGGGGACATTTATTGATACCTACTGTGTGCTGGGTGTTACAGCTACTA1500.1093549515922081No Hit
ATTATGGGGATCCTTTCTTGCCTCTTCCTGTCTTCTGGTGGCTGCTAGAAGT1500.1093549515922081No Hit
ACTCCAGGGATAATCAATGATGTTAAACTTTCTATACTACCCAAAGAATTCT1500.1093549515922081No Hit
CAGTAAGGGAAGAAAACTTAGTACTACTAGTATCTGCCTTATTTTCTTAAGT1500.1093549515922081No Hit
CATTCTGGGTGGTCAATACACAGTAATTCAGATTTTTCAATTATTTCTTTGG1500.1093549515922081No Hit
GCGCTCCGGGCCTAGCACCTCACACAGCGACTGGCAAATAATGGAATGCTCA1500.1093549515922081No Hit
TTACTGGGGACCCTGAAGAGACAAGTTTTAGAAGTATCCTTGCTTTGTGCAT1500.1093549515922081No Hit
TTTTGAGGGACAGCATTAAGCAAATAGACCTAAGTATTTGCCTTTATGAAGC1500.1093549515922081No Hit
CTATAAGGGAAGCAGAAAACAGTGCCCCAAATGGAAAAAAGATACTCACACA1490.10862591858159339No Hit
TAACTTGGGATCGACGGAGGAGTCACCAAGCTCCAGGCAGTGCTCCCACCAG1490.10862591858159339No Hit
TGCCTTGAGTAACTAATAAAATCTCTTACCCAAGAAGCAAGGAGTTTAGAGC1490.10862591858159339No Hit
AGAGGTGGGGTTATTGTGATAAAAACATAACAAAAGTTACCATAGTAACCAT1480.10789688557097865No Hit
ATAAACGGGGGGGCCAGCCTGGAGGCCCAGACGTGGCGCAGCGACTCGGAGG1480.10789688557097865No Hit
TCTGTGGGGACATTCAACTTATATTGAATATCTTAGTGTACCATTCTAAATT1480.10789688557097865No Hit
GTTAATGGGATCCTGCAAATTTCCATCTGCATTTTATTGCCCAGAGATTTCA1480.10789688557097865No Hit
GACCTAGGGATTTATTACAACCTCTTATATGGAAACATCAGTGCTTCACCTG1480.10789688557097865No Hit
TTTGCTGGGCTAACGCAGGGGTTTCTAAGTGAGCTCCCTGAGGTGCCAAGGC1470.10716785256036393No Hit
CGCGCTGGGGCGCTCTCTTGGGGCTGAGGGAGCACCATGCAGCTTCACCCCT1470.10716785256036393No Hit
TATACTGGGACTTGAAGAGTTTATACTCAAACGGCGGTGTAGCCAGACTAGG1470.10716785256036393No Hit
TTAGTAGGGAAGTTAACAAACATATAAGAAATGTATGCACTGTTTGAAATGT1460.10643881954974922No Hit
TGCTGTGGGGCGATCTTAGACGGGCTGTATTTGCAAATCCAACATTGAGCTT1460.10643881954974922No Hit
TTGATAGGGAGAATCAGAAGACCTACTGCTGTTTGCACCTTAAAAAACTAAA1460.10643881954974922No Hit
GCTGCTGGGAAAAGGCATTTTGAAGTTGTGACTACACAAACAGATATCTTTG1460.10643881954974922No Hit
GTATGGGGTTGGTATTAAGACTTCATCTACTGTCTATAGAGTTACAAGCATG1450.10570978653913449No Hit
AAGTCAGGGATATGCTCAGTTTTGTTTTGTTTTGGCAGTTGACAAGAAGTTA1450.10570978653913449No Hit
GTGACAGGGGTGTTGTTCAAGACCCTGTTCAAGTCATTTGGACAAATTTAAT1450.10570978653913449No Hit
TTTTGAGGGTGTGGCTATTGTAAATAAGACTGCATTCTTTATTTAATTCTCA1450.10570978653913449No Hit
GCAATAGGGATTTCAACTTTGTGTTATAGTGAAGTATTTAATATGACACTTT1450.10570978653913449No Hit
TTGAATGGGGTATTTTGGCAAAGGCTTAAAAGGATCATTGAAAGTCCTGACT1450.10570978653913449No Hit
TCGTAGGGGGATCTTTGCTAGGGACCATTAAGATTTAAGTTTTCACGTTTCT1440.10498075352851977No Hit
ACTGCTGGGACCTGGGCAACGTCTGCATCAACTGCCGCCAGCCCTTCATCTT1440.10498075352851977No Hit
ACATCTGGGATTCCATTTAGGCCAGAAATAGATGTTACATATAATTGTAAGT1430.10425172051790506No Hit
GCCCGCCTCGGCCTCCCAAAGTACTGAGATTACAGGCAGGAGCCATCGTGCC1430.10425172051790506No Hit
ATAGGGGATTTTGTTCAACAAATATTTATTACATGCCTCCTCTACATCAGGC1430.10425172051790506No Hit
CTAAGAACGGGATCTGATTCTGTGTCCCACTTCTTACCATTTCTGCCTTCAT1430.10425172051790506No Hit
CTTGAGGGGCCTGCATAGATTCTGAACAATTGGGCCACGAGAAGATTTTGGA1430.10425172051790506No Hit
AGGTTCGGGTTAGTTAGATTCTCATAATGAGCGCAACTTAGACCCCTCACAT1430.10425172051790506No Hit
GCTACAGGGCAATTAACCAGCCTATCTGAAATGACATAATTTCGCACGTATA1420.10352268750729032No Hit
AAAATGGGGGGTAATCAAAACAGCATGATATTGGTATAAAAATAGACACATA1420.10352268750729032No Hit
CACATCGGGGTTGTGTTTTAAATGCTAAAAGACATGCTGTGTTTTAAAATTT1420.10352268750729032No Hit
AACTGCGGGATGGCAGTGCCAAGGGAGCTGTGCAAGTATAATCAAAGCTGGA1420.10352268750729032No Hit
AGCACTGGATTCTAAAGAAAAAAATGGGATTTGTTTTCTCGGCAGATCTGCA1410.10279365449667562No Hit
CATTGCAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAG1410.10279365449667562No Hit
GGTGCCGGGCAACCTCACAGCAGAGGAGATCAAGCGGGAGCAGAGGCTCAGG1400.1020646214860609No Hit
CGATAAGGGATGAAAAGAGCATTAATGAAAATGCTTGTCATGAAAAGCTAGA1400.1020646214860609No Hit
CCCATCCCATCTATCTTGTAAACCAAACAACCAAACTGCATCAGTCGGCTAA1400.1020646214860609No Hit
AAAAGAGGGTAGCTGAATGGTTTTTTTTTAAATGAGTACAACTCTAGCATTT1400.1020646214860609No Hit
ACTACAGGGCAGTCTGGATTCTGTCAGGAAACAGATGATACACTCAAAAGGA1400.1020646214860609No Hit
GGACAAGGGTCTCACAAGGGGATATGAGACCATGAGATTTTTTTTTAAAGTA1400.1020646214860609No Hit
GAGAAAGGGAGGCACATTTCACTGGCATTTTCAATATTCTTTACTTGAGAAG1390.10133558847544616No Hit
ATTAAAGGGGTTTATTGATTTTGTTAAGCAAAGTATTTTGTAGTAAAGATAC1390.10133558847544616No Hit
TGGCTAAGGGGGATGGAAGATGTTACATCTGATTTTGAAAAATATACTTCTC1390.10133558847544616No Hit
GAAATTGGGAAGGCGCTTTGGAAAGGCGAAGCCTGGGCGCAGGGAGCCCAGC1390.10133558847544616No Hit
TTTCATGGGAGAAATGGAAATGCCTGGCACAGCCAGGAAGGAGCTGCCCATG1380.10060655546483145No Hit
CTGCCTCGGGGTATTTGGCAAAGGCACTTGAGAGCAAAATCTTCCTTAGCAA1380.10060655546483145No Hit
TTGCATGCGGGGCGCCCGAAGCCGTCGCGGCGGGGACCATGTTGCTTCCGAA1380.10060655546483145No Hit
CATTATTGGGAATGAATAGATGAGTGAAAGGAGTTTTCTTTTTCTCTTTAGT1380.10060655546483145No Hit
GACAAAGGGGTGCTTTGTGTCTGAATTTCTCAGTATAGACATTTTGTTTACT1380.10060655546483145No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)