FastQCFastQC Report
Sat 31 Dec 2022
SRR5848025.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848025.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211518
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCCTGTATCTCGTATGCCGTCT7390.3493792490473624No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATGCCTGTATCTCGTATGC6050.28602766667612217No Hit
TACAGTGGGACTGGGAGCTGTCCTTTCAGCACCACAAAATTACTGCTTGAAG2390.11299274766213749No Hit
ACAGATGGGAATACTAGAATTGTCTGCAAAATATAGTAAGAAGAATTGGAGC2290.10826501763443301No Hit
GTCGTGGGGCTGTACTTAGTCACGGGGATGGTGGAAGAGGGAGAAGAGGAAG2280.10779224463166256No Hit
GCTAGTGGGCGACGGGGGGACAAAACACATAGCCTACCCCTTCCTTGTACTA2260.10684669862612164No Hit
TAGTGCGGGATGATTTAACAAGTGGAAAGAACATCTCTTTCCTTCCATTTTC2250.1063739256233512No Hit
CAAGTTGGGCGCAAGTCCCATGAAGCTGAGGTCTTGAAGCAGCTGGCTGAGA2230.1054283796178103No Hit
TGCTACGGGCAGTTTTGATTTTTCAAACACTTGGGTGAGATCCAATTTATCT2230.1054283796178103No Hit
TGTGCTGGGGCAGAGTGGGAGAAGTGTCTGAATTTGCCTCTGGATGTGCAAG2220.10495560661503986No Hit
GTAGTTGGGATGAATGGAGATCAACTATACTTGGTCCACCGGGTTCTGTATA2200.10401006060949895No Hit
AGCGTGGGGGAAGTCAACGCTAATCATACACGAACGGACACACTCCGGTGAG2180.10306451460395806No Hit
TCACCAGGGATAAGGTGAATTTTAAAAAATGATTAAGCCCGAGTTTTAAGCT2180.10306451460395806No Hit
AGGTAAGGGAGCCAAGTTTTTTCTAAAACTCTTACGGGAAGGCGTGTGGATG2180.10306451460395806No Hit
GCAAACGGGGCTCAGTTGCTTCCACAGCTCCTGGCTCAGTGCTGCACACACA2120.10022787658733535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)