FastQCFastQC Report
Sat 31 Dec 2022
SRR5848014.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848014.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126353
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGC14591.1547015108465963No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGCCGTCT14551.1515357767524317No Hit
CATGTTGGGGAACCCAGAATGAGAGGCAGAATGCGACAAAAGGATCTAAATG2090.16540960642010874No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTC1990.15749527118469683No Hit
CTGCCAGGGTGGACGGCGACCTGGCGGGCCGATACTACGCGCTCAAGAGCAT1640.1297950978607552No Hit
ACGCACGGGTGCTGCCTGCCATGTGTGAGTCACTTCTACGCATAAACTAGAT1560.12346362967242566No Hit
CCACACAGGTACTGTTTCTCATTTACAGTGAGACCTGGCAAGGATGATGTTG1500.11871502853117852No Hit
TTCAACGGGCACCTTCAAGGAAACATTTTCAAAGAAAATTCCCAGAGTTGTT1490.11792359500763733No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCA1480.11713216148409615No Hit
GCTAGGGGGGCTTCTTTTCCGACAAAACACCAAATGGCGGATGACGCCGGTG1470.11634072796055496No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGT1450.11475786091347258No Hit
TCGCTAGGGTACCTGTAGTATACTATATCATATTGCTTTTTCAGTTTACTTT1400.11080069329576661No Hit
TATTTAGGGGAGCTGTTTTTCGTCTTCCCTAGGCTATTTCTGCCGGGCGCTC1390.11000925977222543No Hit
ATTGGCGGGGACCTGCTCAAGGTCAGTGTCATCTTTGTGTTTAGCTCATCCA1390.11000925977222543No Hit
CTCCACGGGGGCGAGGAGGCGGAAGGAGCAGAGGACCGGCAGCCGGCGTCGA1380.10921782624868424No Hit
AATGCAGGGGCAAAATAAACAGCTCATATTTTACTATGATGATTTAAAAACA1370.10842639272514307No Hit
ACGCTAGGGGTGCGTACGTGCGTCGTCTCTATGGTGGCGGCGGATTTGGAGG1360.10763495920160185No Hit
TGTTAGGGGACTTTAACCTGGATATGGAAAAACCCTGTTGAAGTCTAATTCT1350.10684352567806067No Hit
CACACTGGGGTAGGGAACAAATGTGATCTGACCACAAAGAAAGTAGTAGACT1350.10684352567806067No Hit
AGTGAGGGCTTGTAAGCAGAAAACAAGCCCTCTCTCTTGTGAAGTATCTTCA1310.10367779158389591No Hit
GGCATGGAGGTGCCACACACTTTTCAACAACCAGATCTCATGAGAACTCATT1280.10130349101327234No Hit
TGCTTAGGGGTTTGAAATTAATGTCTAAGCAATTGTGAGAGTGCCAGTAGAA1280.10130349101327234No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)