FastQCFastQC Report
Sat 31 Dec 2022
SRR5848000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81863
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGCCGTCT12171.486630101511061No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGC10991.342486837765535No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTC1620.19789159937945103No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCA1220.14902947607588288No Hit
TTAGACGGGAATTACTTCTGTTAGCAAATGACATGATCTTTTATGTTGAAAA1080.13192773291963403No Hit
ACTGCTGGGTCTGGTTCGGGGGTGTTTTATTTGGTGAAAATTGGAATCCAAA1050.1282630736718664No Hit
TGCATAGGGGACTTTTAAAATTGTGCTTACTTCAGTGCTTATTGTTTGTTTC1010.12337686134150959No Hit
ACAGTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1000.12215530825892039No Hit
CTACATGGGAGCCTCCAAGACAGACCATAGGATAGGCCACAAAAACAAATCT1000.12215530825892039No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCCTGTCTCTTATAC950.11604754284597438No Hit
GTGCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC950.11604754284597438No Hit
GGGCTGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA950.11604754284597438No Hit
AATCATGGGGAAAACAATTCAGTGGTCTTATTTTTGGGTGATTTTTCAAAAA950.11604754284597438No Hit
AACAGCGGGATAATTAATTTGATTGCATGAACTAAGCAATTTTACTAATGAA940.11482598976338516No Hit
GACTATGGGGGACCAATGGCAAATAATGAACTCAGGAGAAACGTACTTAATG940.11482598976338516No Hit
ATAGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA940.11482598976338516No Hit
TATCAGGGGGAGCGGGCAGGTGTAAGACTTGACACTGAGCACAATGTTGTAC940.11482598976338516No Hit
CATGCAGGGCAAACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAAT940.11482598976338516No Hit
CTTCTAGGGAAACCAATATTGTGGTAATCTTATTCTACTCTCACTAATAGAA930.11360443668079595No Hit
TTGCTAGGGGTTGCTGATGAATCTAATAGTTCATTCCTTTTTAAGACCAAGT930.11360443668079595No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGT920.11238288359820675No Hit
TATGGTGGGATGTCCAAAGCTGAGAAGAATGGTGTGAAGATTACCTTGCCTG920.11238288359820675No Hit
GCTTGTGGGTGGAACTCACTAGGTTCCTTTTAGATACGGCATAGAATTCTAT910.11116133051561755No Hit
TCGAACGGGTGTAAGAGAAATAATAAATAAGATTCATTAAAATGCTAGAAAC910.11116133051561755No Hit
GCTCATGGGAGTGGAATTGACTAAGAAACTTGCCAGGTCTTTACCTTTTAGT900.10993977743302835No Hit
CTATCTGGGGTCCTTGATCACACTAAAATAAAATTGCTTATTTACTTTTCTG900.10993977743302835No Hit
CATCAAGGGAGCTGTGGTGATCACGTTTTGCCTGCCTGTGCCCATGTCTCTT880.10749667126784994No Hit
GTCAACGGGCTTTGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTT880.10749667126784994No Hit
TTGTTGGGGCAGTTAGACATTCTAGAATATTTCACAAAATAAGAATTTTGCA880.10749667126784994No Hit
ATAACAGGGCTTGGCCTCAGGTGATCCTCCCACCATGGCCTCCCCAGATGCT880.10749667126784994No Hit
GTAGCGGGGATGATTTAATGAGTAATGTTAAGTACAGAAAAGCTGCCATATC870.10627511818526074No Hit
GTCAATGGGTGAATTTCAGTGACATAAATGGTAATAGAAAGAGGGTCAACTA870.10627511818526074No Hit
GTTCCAGGGAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAAC870.10627511818526074No Hit
GAACACGGGTACGCCAGGAATTTTGAGACTTCTCATCTTTAAATTCAACCAA860.10505356510267154No Hit
GCCAATGGGTAAACAGAAAAATATCATAGTTCTTGATTTCCCGCGCATATGC860.10505356510267154No Hit
TTTTAGGGGACCTGAATCACCTGTTGCTCTCTTTCTGTTTTGTACTTTTTTT860.10505356510267154No Hit
AATCTAGGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCA860.10505356510267154No Hit
CGTTAGGGGACCAGCAAAAGCACATTAGACAGGGGTAAGGTTTGTTATACAG850.10383201202008234No Hit
GTTCAGGGGATTAGCAAGTATGCACAAAAGCCAACTGTTTTAAAGCTCAAGG850.10383201202008234No Hit
CGACTAGGGTATAGTATCATACAGAATGGTTTTACTGCCGTGAAGATTCTCT840.10261045893749314No Hit
CTCGCTGGGAGGTGACCACCATGGATGAGGCCTGTCAGGAGGGCAACATCTT840.10261045893749314No Hit
TACAACGGGGGATCAGACACGGCACCTTCAGTTTTGTACTATTGGCTTTGAA840.10261045893749314No Hit
TTTGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC840.10261045893749314No Hit
TGTGTAGGGTGTGAAATTGCTCTTTGAAAATTTCTTTTTACACGTGTAAGCC830.10138890585490393No Hit
ATAACCGGGCTATCTGTATTGGCAGCACAGCTACTCAGATCTGCACTCCTGT830.10138890585490393No Hit
TTATTGGGGCTGCAACCCTGGATGTTGAATTAGTGAATAAACAAACTAGGTC830.10138890585490393No Hit
TATACAAGGGAATTTTAATGGAAATAACTACTGTAGACTATTGAAGAATGAT830.10138890585490393No Hit
GCCCTAGGGGAAAAAGGTAAGATTTAGAAGGAAGGAGGATTTTATAGGCAGA830.10138890585490393No Hit
CTCTCAGCGGGTGGCCAAGCATGCAGCTTCTACCGTTCAGATTCTTGGAGCT830.10138890585490393No Hit
CACGTCGGGAGAGACCTTACAATAAATGGAATGACTTAAAATAAATGGAATA820.10016735277231473No Hit
AACCTAGGGTACACTATGCTGTACAACTGCCTGGACTTCCCTGCAGGGGTGG820.10016735277231473No Hit
TCTGTGGGATTTCACTGTCTAGGCTACAACAGGATTCTAGGTGGAGGTTGTG820.10016735277231473No Hit
TGACATGGGACTTCTGAGTTTGACTTATTTAGGTTCTACATATAAGTGGGGT820.10016735277231473No Hit
TCAAAAGGGGAAAAAATGCAATTTTACCAGTGATCAAAGAAATGCAAGCTAA820.10016735277231473No Hit
TCATTTGGGCTTTCAGGAAATCACACAGGAGCCCAGGGCTTCCCACCAGGGA820.10016735277231473No Hit
TTTACAGGGATGGTTTAAAGATAGGGGTTTTGTTCTACTTTTTTATTTTATT820.10016735277231473No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)