FastQCFastQC Report
Sat 31 Dec 2022
SRR5847999.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847999.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82384
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGCCGTCT11581.4056127403379297No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGC10181.235676830452515No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTC1460.1772188774519324No Hit
TTAGACGGGAATTACTTCTGTTAGCAAATGACATGATCTTTTATGTTGAAAA1110.13473489998057875No Hit
ATAGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1080.13109341619731987No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCCTGTCTCTTATAC1070.12987958826956691No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCA1030.12502427655855505No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGT1030.12502427655855505No Hit
GGGCTGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1030.12502427655855505No Hit
GTCAATGGGTGAATTTCAGTGACATAAATGGTAATAGAAAGAGGGTCAACTA1010.12259662070304912No Hit
GAAGTTGGGTGGTAAGATGTCATAATGGACCAGTCCATGTGATTTCAGTATA990.12016896484754322No Hit
ACTGCTGGGTCTGGTTCGGGGGTGTTTTATTTGGTGAAAATTGGAATCCAAA980.11895513691979026No Hit
GTTCAGGGGATTAGCAAGTATGCACAAAAGCCAACTGTTTTAAAGCTCAAGG980.11895513691979026No Hit
GTTCCAGGGAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAAC970.11774130899203727No Hit
GTCAGGGGACATGCTATATTGATAGTTATTTGACGAGGTCATTTTGACGTTG950.11531365313653136No Hit
ATCCATGGGCAAGGCAGGAGAGATGGAAGCCGCCCAGAGAACTGCCACTCCA940.11409982520877841No Hit
GTGCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC930.11288599728102545No Hit
TTTCAGGGGTCACCCCTGGGAGATACATGCCTGCTTACCAGGACCAATTTTC920.11167216935327247No Hit
TAGCCAGGGGAAAGAATGGAGCCTATCACTTTTGGCAGTACTTCATGAGCAT910.11045834142551951No Hit
TTGCTAGGGGTTGCTGATGAATCTAATAGTTCATTCCTTTTTAAGACCAAGT900.10924451349776657No Hit
ACATTAGGGTGACATAAGTAGCAGCCGTTTTTCAACTTCAGTTTCATTCACT900.10924451349776657No Hit
AAAGTGGGGAGAGTACATATGAGTCAAATACCAAACAGCTATCATATGATGA900.10924451349776657No Hit
GTTCTAGGGACCGCGAAGGGAGGAGTGGCAACATGGCGTCTTCGGGAGCTGG900.10924451349776657No Hit
ATTGCAGGGGTTAGTGAGGAGTTCTGATATTAAGCACACACACACATGCACA900.10924451349776657No Hit
GCAAGTGGGCAGTAGCTGCATGGTGTCAACCAGTGCTTTTCTCCTTTGCTTT890.10803068557001361No Hit
TCGAACGGGTGTAAGAGAAATAATAAATAAGATTCATTAAAATGCTAGAAAC890.10803068557001361No Hit
ATAATCGGGCAGAAGAAGGAGGAGGAGTCGGCGGGCGGCACCAAGGGCAGCA890.10803068557001361No Hit
CTACATGGGAGCCTCCAAGACAGACCATAGGATAGGCCACAAAAACAAATCT890.10803068557001361No Hit
GAAGTGGGGGCTTTCTCACGTACTATGACCCAGCAGCCTTAGCATAACAGTA880.10681685764226062No Hit
TTGTTGGGGCAGTTAGACATTCTAGAATATTTCACAAAATAAGAATTTTGCA880.10681685764226062No Hit
CTCGTAGGGACACTATGCCAGTGCCAAGGTATGCGAGGAGAAGCTCCGCTAT880.10681685764226062No Hit
TATGGTGGGATGTCCAAAGCTGAGAAGAATGGTGTGAAGATTACCTTGCCTG880.10681685764226062No Hit
TATACAAGGGAATTTTAATGGAAATAACTACTGTAGACTATTGAAGAATGAT880.10681685764226062No Hit
GTCCAAGGGGAGGGAGTTAGCATCACCTAAAACCTGCACGTGAACAAGGGTT870.10560302971450766No Hit
TTATGGGGAAGCCGCCCTTGAAGAAAACGCAGCAAAATATTTTAAAATGAAG870.10560302971450766No Hit
CTAATAGGGGGAGCTCCTCACTGGACTCGCCTCTCTCCTCTCCTCCCCGCAG860.1043892017867547No Hit
GCTTGTGGGTGGAACTCACTAGGTTCCTTTTAGATACGGCATAGAATTCTAT850.10317537385900176No Hit
GTCATTGGGTAATGATATTTTGCTATAAGCAAGAGCAGAAATTAATAAAGAA850.10317537385900176No Hit
TCTGTGGGATTTCACTGTCTAGGCTACAACAGGATTCTAGGTGGAGGTTGTG850.10317537385900176No Hit
ACACATGTCCCTGGCATCTGTTCAGAGCAGTGTGCAGAATGTAATGCTCTTT850.10317537385900176No Hit
GCAGCGGGCAAGAGGCTTAATTTACCAATTTAAATAGCCACAGTCCTTAAGC850.10317537385900176No Hit
TTGCGAGGGACACGCATGCATGCACAGCCAGATGGCCACTCAGCACAGATGT850.10317537385900176No Hit
GAACACGGGTACGCCAGGAATTTTGAGACTTCTCATCTTTAAATTCAACCAA840.10196154593124877No Hit
CACGTCGGGAGAGACCTTACAATAAATGGAATGACTTAAAATAAATGGAATA840.10196154593124877No Hit
TGTGTAGGGTGTGAAATTGCTCTTTGAAAATTTCTTTTTACACGTGTAAGCC840.10196154593124877No Hit
CGAAAAGGGACTCTTTCACAATTAAGGAAATGGGACAGAGTAGAGTCAAGAG840.10196154593124877No Hit
ATTAACGGGATTGCAGGGAATGTTTTTACTTAGCTTTATAAACAGCACATTT830.10074771800349581No Hit
CATCAAGGGAGCTGTGGTGATCACGTTTTGCCTGCCTGTGCCCATGTCTCTT830.10074771800349581No Hit
TAAGCGGGATAATACATTTTCATAAAATCCTTCTTTCGATGCCCTTTTTTTT830.10074771800349581No Hit
TTTCAAGGGAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCC830.10074771800349581No Hit
GTCCAAGGGATGTCTTTCCGATGGTGTCTCCCAAGTGTTGGTGCTTTGGGTT830.10074771800349581No Hit
TCAAAAGGGGAAAAAATGCAATTTTACCAGTGATCAAAGAAATGCAAGCTAA830.10074771800349581No Hit
GCATATGGGATTGTGATAATAAGAAAGAAGAAAAAGAAAGAAAAGTGGTAGC830.10074771800349581No Hit
GTTAGAGGGAACTTGACATTGATAACACAGTTTGATGCTTTTAATTTTCTGT830.10074771800349581No Hit
ATAACAGGGCTTGGCCTCAGGTGATCCTCCCACCATGGCCTCCCCAGATGCT830.10074771800349581No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)