FastQCFastQC Report
Sat 31 Dec 2022
SRR5847998.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847998.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81749
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGCCGTCT12231.4960427650491137No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGC10681.306437999241581No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTC1530.18715825270033884No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCA1330.16269312162839913No Hit
GTTCAGGGGATTAGCAAGTATGCACAAAAGCCAACTGTTTTAAAGCTCAAGG1140.13945124711005638No Hit
TATGGTGGGATGTCCAAAGCTGAGAAGAATGGTGTGAAGATTACCTTGCCTG1080.13211170778847448No Hit
TTAGACGGGAATTACTTCTGTTAGCAAATGACATGATCTTTTATGTTGAAAA1050.1284419381276835No Hit
AATTAAGGGTACAGAACAAACCAACAGGACAGTGATATCGAAGCGTTGGCCA1030.12599542502048955No Hit
GTTCCAGGGAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAAC1030.12599542502048955No Hit
AACTTGGGGATGCAGCTTCTCTTGCAGTGCAGGCCTTTTTGCAAATGTGTAA990.12110239880610162No Hit
TTGCTAGGGGTTGCTGATGAATCTAATAGTTCATTCCTTTTTAAGACCAAGT980.11987914225250462No Hit
TGACATGGGACTTCTGAGTTTGACTTATTTAGGTTCTACATATAAGTGGGGT970.11865588569890764No Hit
GTCGCTGGGTGTCCTACTGAATGCTGCTAAGTGCTTAGTGTTTCACAAAAGC970.11865588569890764No Hit
TTTTAGGGGACCTGAATCACCTGTTGCTCTCTTTCTGTTTTGTACTTTTTTT960.11743262914531065No Hit
GCTCATGGGAGTGGAATTGACTAAGAAACTTGCCAGGTCTTTACCTTTTAGT940.11498611603811668No Hit
TTGCGAGGGACACGCATGCATGCACAGCCAGATGGCCACTCAGCACAGATGT930.11376285948451968No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCCTGTCTCTTATAC920.1125396029309227No Hit
CTATCTGGGGTCCTTGATCACACTAAAATAAAATTGCTTATTTACTTTTCTG910.11131634637732571No Hit
TACCAGGGAGACAAAAAAGAAGAAAACTACCTTCTGATCTTGTATTTTGAAA900.11009308982372873No Hit
CACGTCGGGAGAGACCTTACAATAAATGGAATGACTTAAAATAAATGGAATA890.10886983327013174No Hit
AACAGCGGGATAATTAATTTGATTGCATGAACTAAGCAATTTTACTAATGAA890.10886983327013174No Hit
GTTAGAGGGAACTTGACATTGATAACACAGTTTGATGCTTTTAATTTTCTGT890.10886983327013174No Hit
GTGAATGGGATTTGTGGTTAGAGTTATTCATTGTTTTCATTTTTTAAACTTG890.10886983327013174No Hit
TTTCAAGGGAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCC880.10764657671653476No Hit
TGCATAGGGGACTTTTAAAATTGTGCTTACTTCAGTGCTTATTGTTTGTTTC870.10642332016293776No Hit
TGTGTAGGGTGTGAAATTGCTCTTTGAAAATTTCTTTTTACACGTGTAAGCC870.10642332016293776No Hit
TCGAACGGGTGTAAGAGAAATAATAAATAAGATTCATTAAAATGCTAGAAAC870.10642332016293776No Hit
CGACTAGGGTATAGTATCATACAGAATGGTTTTACTGCCGTGAAGATTCTCT870.10642332016293776No Hit
GCAAGGGTGATAATCAGCTGAAAGACTCTGAATCTACGCCTGTGGATGATCG870.10642332016293776No Hit
CTCTCAGCGGGTGGCCAAGCATGCAGCTTCTACCGTTCAGATTCTTGGAGCT870.10642332016293776No Hit
TTGCTTGGGGACCATGGGCTGAGTTCTTTCTCTGGGTCCCCTAATGTGGGAA860.1052000636093408No Hit
CTCTGCGGGAGTGGCGGGGTGGCATTTGGAAAGAAAATCCTAGAAAGCCTAT860.1052000636093408No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGT860.1052000636093408No Hit
GTTACTGGGTGGTTTTTCACTCTCCAACACACCAGATGTACAGCTTCCACAT860.1052000636093408No Hit
TTTGGCGGGACTGGCCAATGCTTCACCAGTTAGTCATGCTCCAAGGATTGAG860.1052000636093408No Hit
ATGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA860.1052000636093408No Hit
AATCATGGGGAAAACAATTCAGTGGTCTTATTTTTGGGTGATTTTTCAAAAA860.1052000636093408No Hit
GTCAATGGGTGAATTTCAGTGACATAAATGGTAATAGAAAGAGGGTCAACTA850.1039768070557438No Hit
TTAGCCGGGCACCCACATCCATAACACACCAGGGTGCATTCTTGATGTAAGG850.1039768070557438No Hit
GTGCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC840.10275355050214682No Hit
TGGCTGGGCAGTATTCTAAGCACTTTACACTACTTTAGAAAACTCGTTTAAT840.10275355050214682No Hit
TCTGTGGGATTTCACTGTCTAGGCTACAACAGGATTCTAGGTGGAGGTTGTG830.10153029394854983No Hit
GTAGCGGGGATGATTTAATGAGTAATGTTAAGTACAGAAAAGCTGCCATATC830.10153029394854983No Hit
CTGTAGGGGGAGAGCAGCTGTTGGAAGCTTTGGAGCTTTTGGGCAACTTCAA830.10153029394854983No Hit
ACTGTAGGGGATCTGTGTATGGGCATTCTGAGGTAGGTTTAGTGAGATTATT830.10153029394854983No Hit
ACTGCTGGGTCTGGTTCGGGGGTGTTTTATTTGGTGAAAATTGGAATCCAAA820.10030703739495285No Hit
AAGCTTGGGTCTGCTGTAGGGCTTTTGAGAGCTGACATCTGATCACTGAGCT820.10030703739495285No Hit
TCTATTCGGGATAAGGTAAAATTTTCCTGTTTTTCCCCCTTTGAAAAACTCA820.10030703739495285No Hit
GGGCTGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA820.10030703739495285No Hit
TTAAAAGGGATTCAACGGGAATAAAACTGGCATGGTAATTTTTTTTTTTTTT820.10030703739495285No Hit
ATACTCGGGGGACACAGGTCAAGGGTCAGGGCACTGTCAAGGGGAAGTTATT820.10030703739495285No Hit
GCAGCGGGCAAGAGGCTTAATTTACCAATTTAAATAGCCACAGTCCTTAAGC820.10030703739495285No Hit
ATAACAGGGCTTGGCCTCAGGTGATCCTCCCACCATGGCCTCCCCAGATGCT820.10030703739495285No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)