FastQCFastQC Report
Thu 19 Jan 2023
SRR5847997.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847997.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208017
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGC12660.6086041044722306No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGCCGTCT12120.5826446876937942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGT253.398298E-546.0209241
TATGGCG253.398298E-546.0209241
CTATACG351.01055775E-746.0209241
TTACTCG253.398298E-546.0209241
TCGCTAG253.398298E-546.0209241
CATACCG253.398298E-546.0209241
CATTTCG253.398298E-546.0209241
GCGTAGG253.398298E-546.0209241
TGCCACG206.285374E-446.020921
GCAAACG206.285374E-446.020921
AAACGTG206.285374E-446.020921
AAACGCG206.285374E-446.020921
CGCTAAG301.848206E-646.020921
TCGTACG405.5515557E-946.020921
CCGACAG206.285374E-446.020921
TATAGCG206.285374E-446.020921
ATAACCG206.285374E-446.020921
ATCGCTG206.285374E-446.020921
ATCGCAG206.285374E-446.020921
AACGGGT453.074092E-1046.0098574
Produced by FastQC (version 0.11.5)