FastQCFastQC Report
Sat 31 Dec 2022
SRR5847994.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847994.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76322
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGC6790.8896517386860932No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGCCGTCT6370.8346217342312832No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGT1650.21618930321532456No Hit
TGTGCTGGGGAAGTTGAAAATTGGTACGAGTTACTTAAAGTCCCTAGGTTTT1580.20701763580618956No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTC1110.1454364403448547No Hit
TTGTACGGGGGTCGCCAGGAGGAGCGCGCGGGCACAGGGTGCCGCTGACCGA1090.14281596394224472No Hit
CATGCCGGGTAAATACTTCTCCCCACCCCGAATAAGCGTATTCTTCTGGGAA1020.13364429653310972No Hit
CGAGTTGGGGAAGCCAAGGAAGTAGTGCAACTATAATTTAAATGTTACTAGA1010.13233405833180473No Hit
GTCCTGGGGATATACACATCATGTTACATAATGGTACCACAGTCTACAGTTC990.12971358192919472No Hit
TGCAAGTTTAACTGAAATCATTCCCCTGCAAGAAGGAAAATGTAAAATGGCT940.12316239092266974No Hit
TCCACAGGGATCCCGAGGTGTGCTTGGACCTACGTCCGGGTACCAACTACAA930.12185215272136475No Hit
CGTGGCGGGCTGAACCTTTTGATCTTGTTTTTTAAAAGTGTAAATAGATAGT920.12054191452005976No Hit
AAAATAGGGCTACTCTAAGGGCACTGAAAATGCCCTGGAAGCAGGAGAGAGG880.11530096171483976No Hit
TCAGTCGGGAAGAAAAGAAAAAGGGAAAGGAAAGGTATAAAGGAATTCGGAG860.11268048531222975No Hit
GCATCAGGGATCCAGCATGTTCCTACTCATTCTGCTAGTGCAGCTGGAGTGA860.11268048531222975No Hit
AATCACGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG850.11137024711092476No Hit
ATACCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC850.11137024711092476No Hit
TGACTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC840.11006000890961976No Hit
GTCAATGGGTGCCGTCGTAGGTATTAGTGCTAGTCTCAAATAGGTGCTTCCC810.10612929430570478No Hit
AAGCATGGGCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAA800.10481905610439977No Hit
ATATGGCGGGATAGAGATTAATACAACTACTTAAAAAATATAGTCAATAGGT800.10481905610439977No Hit
TTCTTGAGGGAGTATAGATTTTATGTGATGCTAAGGAGCCCCCGGAAGCTGC800.10481905610439977No Hit
TCGTTTGGGACCACGGAGGAGGACCACCCCTTTGCTGTGAAGTTGCTTGCGC790.10350881790309478No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCCTGTCTCT790.10350881790309478No Hit
ATGTGTGGGGATCTTCAGTAGGGGAGGCAGGCATTGAGGCAGCCAGCGCAGG790.10350881790309478No Hit
AAACATGGACTGAAAAAGGGTTGTATTTTCTTAGCTGAATATGAATACCTCT780.10219857970178979No Hit
AATGCTGGGAATAGGAAGAGAGGGAGTCAAATTGTCTCTGTTTGCAGATGAC780.10219857970178979No Hit
CTTTAGGGGGTAAGTAAACTTCTGATTTTGTTCTTGCTCAGACTTGACAGAT780.10219857970178979No Hit
GAAACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA770.10088834150048478No Hit
ACTACCGGGATCTCATCAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTT770.10088834150048478No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)