FastQCFastQC Report
Sat 31 Dec 2022
SRR5847993.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847993.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76393
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGC6680.8744256672731795No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGCCGTCT6480.8482452580733837No Hit
TGTGCTGGGGAAGTTGAAAATTGGTACGAGTTACTTAAAGTCCCTAGGTTTT1530.2002801303784378No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGT1460.19111698715850928No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTC1440.1884989462385297No Hit
CGAGTTGGGGAAGCCAAGGAAGTAGTGCAACTATAATTTAAATGTTACTAGA1160.1518463733588156No Hit
TGATTTGGGCGCTGCTCTGCACCGAGCTGGTGGGCTTGGGTTTTGTGGAGCG1060.1387561687589177No Hit
GTCCTGGGGATATACACATCATGTTACATAATGGTACCACAGTCTACAGTTC1010.13221106645896877No Hit
TGCAAGTTTAACTGAAATCATTCCCCTGCAAGAAGGAAAATGTAAAATGGCT970.1269749846190096No Hit
GCATCAGGGATCCAGCATGTTCCTACTCATTCTGCTAGTGCAGCTGGAGTGA960.12566596415901982No Hit
TCAGTCGGGAAGAAAAGAAAAAGGGAAAGGAAAGGTATAAAGGAATTCGGAG940.12304792323904024No Hit
GCTCTGGGGCTGCTGCGGGGTTTGCACACGCTCCTGCACCCTCTCCCCGTCC910.11912086185907086No Hit
AATCACGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG900.11781184139908107No Hit
TCCACAGGGATCCCGAGGTGTGCTTGGACCTACGTCCGGGTACCAACTACAA900.11781184139908107No Hit
CTTTGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC900.11781184139908107No Hit
TTGTACGGGGGTCGCCAGGAGGAGCGCGCGGGCACAGGGTGCCGCTGACCGA870.1138847800191117No Hit
CGTAATGGGCCTTCCTTTCCTTTCAGCGGAGCGCGGCGGCAAGATGGCAGTG870.1138847800191117No Hit
TGACTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC860.11257575955912191No Hit
CATGCCGGGTAAATACTTCTCCCCACCCCGAATAAGCGTATTCTTCTGGGAA860.11257575955912191No Hit
ATGTGTGGGGATCTTCAGTAGGGGAGGCAGGCATTGAGGCAGCCAGCGCAGG850.11126673909913212No Hit
CGTGGCGGGCTGAACCTTTTGATCTTGTTTTTTAAAAGTGTAAATAGATAGT840.10995771863914233No Hit
GATTATGGGATGGTTGATGCAATATTTTAACACTTGCTGATGTGATTCTCAA840.10995771863914233No Hit
TTCAATGGGGCATTGATAAATATTACAAAACTGAATGCAAATGTCCTTTGTG830.10864869817915254No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCA820.10733967771916275No Hit
TAGCTGGGATTACAGGCATGAGCCACCACACCCAGCCACAAATAGAAATTTT820.10733967771916275No Hit
TCGTTTGGGACCACGGAGGAGGACCACCCCTTTGCTGTGAAGTTGCTTGCGC810.10603065725917296No Hit
GCAAAGGGGTCTGATTAGTGACATAAGTAGCAGCCGTTTTTCAACTTCAGTT800.10472163679918318No Hit
TATCCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC800.10472163679918318No Hit
GCGCTAGGGGTTGGCTTCATGTGTTTGGAAATATTTGATGATGTTTCGTCAT800.10472163679918318No Hit
AGGCCTGGGCCGCGAAGGATCTTCTGCCACAGCTGCAACATGGGCGGCAAGA800.10472163679918318No Hit
GCACATGGGGTGCGGCTCTACACGGAGCCGCTGCTGGTGAACCTGCCGACAC800.10472163679918318No Hit
AAACAGGGAACAGAACCTTCTTACGAACTTGTGAAGAGTCAGCAGTGGGATG800.10472163679918318No Hit
GAAACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA790.1034126163391934No Hit
AATTGTGGGAGACCACAATGCTGATATATCTTATTTAATGCAGACTGTATTC780.1021035958792036No Hit
AATGCAGGTCTGTTTCTTTGTTATATACACAGCATGTTACAGAGATGTGATT780.1021035958792036No Hit
AAGCATGGGCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAA770.10079457541921381No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCCTGTCTCTTATAC770.10079457541921381No Hit
GGCCTAGGGACCGTGGGTCTGAACATGACTTGTTGCTGTTGCTTTGGAGCTT770.10079457541921381No Hit
GTAATCGGGTATAGAGAAATGTTTTGAGATTCAGTGGTAAAGTCATTTCATG770.10079457541921381No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)