FastQCFastQC Report
Sat 31 Dec 2022
SRR5847992.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847992.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74855
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGCCGTCT6550.8750250484269587No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGC6450.8616658873822723No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGT1810.2418008149088237No Hit
TGTGCTGGGGAAGTTGAAAATTGGTACGAGTTACTTAAAGTCCCTAGGTTTT1400.1870282546256095No Hit
TTCTTTGGGCAATAAATTTCCTCAAGTTGTATTTGTATTTTAACAGAATAAT1160.15496626811836217No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTC1150.15363035201389352No Hit
TTGTACGGGGGTCGCCAGGAGGAGCGCGCGGGCACAGGGTGCCGCTGACCGA1120.1496226037004876No Hit
CGAGTTGGGGAAGCCAAGGAAGTAGTGCAACTATAATTTAAATGTTACTAGA1120.1496226037004876No Hit
GTCCTGGGGATATACACATCATGTTACATAATGGTACCACAGTCTACAGTTC950.12691202992452075No Hit
CTTGCTGTGGAGTGTATGCAGGACAGATTTTAGAAACTTAGATTAAAATACA940.1255761138200521No Hit
GCATCAGGGATCCAGCATGTTCCTACTCATTCTGCTAGTGCAGCTGGAGTGA930.12424019771558345No Hit
CGTGGCGGGCTGAACCTTTTGATCTTGTTTTTTAAAAGTGTAAATAGATAGT920.12290428161111482No Hit
GCTCTGGGGCTGCTGCGGGGTTTGCACACGCTCCTGCACCCTCTCCCCGTCC880.11756061719324026No Hit
CTTTAGGGGGTAAGTAAACTTCTGATTTTGTTCTTGCTCAGACTTGACAGAT870.11622470108877163No Hit
TCGTTTGGGACCACGGAGGAGGACCACCCCTTTGCTGTGAAGTTGCTTGCGC860.11488878498430298No Hit
GCGCTAGGGGTTGGCTTCATGTGTTTGGAAATATTTGATGATGTTTCGTCAT840.11221695277536571No Hit
TCAGTCGGGAAGAAAAGAAAAAGGGAAAGGAAAGGTATAAAGGAATTCGGAG830.11088103667089708No Hit
GTAACCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC810.1082092044619598No Hit
TCCACAGGGATCCCGAGGTGTGCTTGGACCTACGTCCGGGTACCAACTACAA800.10687328835749116No Hit
ACTACCGGGATCTCATCAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTT800.10687328835749116No Hit
AAAATAGGGCTACTCTAAGGGCACTGAAAATGCCCTGGAAGCAGGAGAGAGG790.10553737225302252No Hit
AAACATGGACTGAAAAAGGGTTGTATTTTCTTAGCTGAATATGAATACCTCT780.10420145614855388No Hit
GGCTTAGGGGTGGTGGCCATTTGTATGAAATGTCCAGAATTGGAAAGAAAAA780.10420145614855388No Hit
ATCAGTGGGGTTTATTCTGGTAATACAAGGATGGTTCAGTATTTGAAAATCA770.10286554004408524No Hit
TTCAATGGGGCATTGATAAATATTACAAAACTGAATGCAAATGTCCTTTGTG770.10286554004408524No Hit
TAGCTGGGATTACAGGCATGAGCCACCACACCCAGCCACAAATAGAAATTTT750.10019370783514794No Hit
TGCAAGTTTAACTGAAATCATTCCCCTGCAAGAAGGAAAATGTAAAATGGCT750.10019370783514794No Hit
TTTGAGGGGAGAGAAAATTCAGTAATACCTGTCTATTAACATCACTATCACT750.10019370783514794No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)