FastQCFastQC Report
Sat 31 Dec 2022
SRR5847985.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847985.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51607
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGCCGTCT12642.4492801364156027No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGC11952.315577344158738No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTC1920.37204255236692696No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCA1200.23252659522932936No Hit
ATTCATGGGGACTTCATTCATTATAATGATTTCTGTTCAGCATCTCCAGTAT1020.19764760594492997No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCCTGTCTCT960.18602127618346348No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCCTGTCTCTTATAC900.17439494642199702No Hit
GTAATTGGGTGCATCCAATTGCTTGAACCATTTAAGTAGAGTTGACTGATTT870.1685817815412638No Hit
TCGCCTAGGGGCAGTATAGTGTCACCTCAACCTCAAATGATATGGCTCATTG850.16470633828744163No Hit
GGTACTGGGCGATCTTAATCAGGGACGATAGATGTTATTTTATAAATTTCAA820.1588931734067084No Hit
GCTACCGGGGAGAAGCAGTCAGAGGGATTCTTGCATTGTGATGAGTGCATAT820.1588931734067084No Hit
GGCTCAGGGGCTGATACCAAAACCTGGCAGACACAACAACAAAAAAATACAA810.15695545177979733No Hit
AGATAGTGGGATCTGCAGTGGAACTAAAGAGTATCAATCAAAGTGATGAAAA810.15695545177979733No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGT790.15308000852597517No Hit
GCTCGTGGGGATATGATTTTTCTTCAGGGAACAGAGGTCATATTTAAAGTGG790.15308000852597517No Hit
ACTGCTGGGATTACAGGCGTGAGCCATCATGCCCAGCTGATCATCCCCATCT780.1511422868990641No Hit
TTCCTTGGGGCCGCCGTCGCCGCCATGACGGGGCTAGCACTGCTCTACTCCG760.14726684364524192No Hit
TTGCGCAGGGCTCGCTCCGGGGGGAATCATGGTCATTCCATGGAGACCTCCC750.14532912201833084No Hit
CCACCAGGGCCTACCAGAGAGTGGAGGGTGCAAAGAAGGAGGGGATCAGGAA730.14145367876450868No Hit
CTTTAAGGGGAAGGGCACTGAAAATGCCCTGGAAGCAGGAGAGAGGGACTGG720.1395159571375976No Hit
GTTGCAGGGTCTATTATTGGGATGGATTGAAAGATAATATTGTTAAAATGTC710.13757823551068654No Hit
AGGTGGGGGTTCAGGCTGGTCTTGAGCTCCTGGCGTCGGGTGGTCCTCTTGC700.13564051388377546No Hit
ATTTGTGGGCGGGATTGGCTAAACTCCCATAGTATTTATGGTGGCCGCCGGC690.13370279225686438No Hit
TGTATCGGGATTGTTGTAAAATGAGGAATGTATGTGAATATTCAGATAACCG680.1317650706299533No Hit
AAGTGGGCAGTGTCAGCATTAGGGTTTTGGTTTTTGTTTCTTTTGGGTATTT680.1317650706299533No Hit
GAATTAGGGAAGTAGAGGTACTAGTGGAATATCCTTTACGTTGTCTGGTGTT680.1317650706299533No Hit
CAACAAGGGATTGGCCTTACAGTGGGCCATTCATTTCTATAGGCAAAGAAAG670.12982734900304221No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCAACTCGTATGC670.12982734900304221No Hit
CGGCACGGGAGGAGTAAAACCCTATGAATGTAGTGAATGTGGGAAAACCTTC670.12982734900304221No Hit
AGTGGGGGAGGATTGGGTTCAGGCATGAATAACAAAATCCCGAAACAATGGT660.12788962737613113No Hit
TGGTCTGGGAAATTCTGTGAACCAGAATGAGACAAGGCAGCAGGCATACAGA650.12595190574922005No Hit
CGACAGGGATGATAGATAGATAGATTAGATAGATAGATAGATACATACATAC650.12595190574922005No Hit
AGCAACGGGGCCAGAGGCAGCTATTTCTATGGCTCCTTTTGCTGCTGAGGAC650.12595190574922005No Hit
TTGCCGGGGACCGGAGGGCACTGAAGCCTTGCCACTTGCTCAAGTGGGGCTG640.12401418412230898No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCAT640.12401418412230898No Hit
GGCAGCGGGCTTTGATGGGGACTTTAATCCAGTGGCTCCTAACCAGGGGTCA630.1220764624953979No Hit
GGGCACGGGCCCACTTGTTGCTATCTTACCTGTCAAAGAAGCACTGAGTCAC630.1220764624953979No Hit
GCAGCAGGGATACCATGTTCTTCCTTTTGGCTTAGTTTTGCCATGCATCCTG630.1220764624953979No Hit
GGACTAGGGAAATTGAATAAATTTGCTTATTAGTTTTAAAATATTTAAGATT630.1220764624953979No Hit
TGTAAGGGCAAATTGAAGTCCCGGAAAAAGCAAAATTTTTTTAAACTTTTTA630.1220764624953979No Hit
CATCAAGGGGAGCTCCCCTTCCTCCAGGCTCATGATGGTGTTGCTAAGGAAT620.12013874086848685No Hit
GGGAAGGAACTCGTTTATTGATTTATTGAAGATCTTTGGCTGTTTTTGATAA620.12013874086848685No Hit
CGCACTGGGCACACGTTACAGAAGCTACGCATGGATCTGATCCGTTTACAGC610.11820101924157576No Hit
GGAACTGGGATCCCCATGCATTTGAGAACCTTTGCATAGACTGTACTGCAGC610.11820101924157576No Hit
TCCGCCGGGTATCAACAGCAGAATAAAGAAAGATTACATATGTAAAAGTACC600.11626329761466468No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCACTGTCTCTTATACAC600.11626329761466468No Hit
GTCTATGGGATATATCACCCTGTCGACTATATTTATGGATTTATTACTGGGT590.1143255759877536No Hit
ACTGTTGGGGAACCAAGGATATTGGAGGCTAGTCAAAGGCTACAAGGACATC590.1143255759877536No Hit
ACTCGCGGGATATTTTTGTGGAGAGAAAGACACCAAAGAGTACATACTATAT590.1143255759877536No Hit
CTAGCTGCGGGGCCCTTCCTGGGCATCGTGGCTGAGGTGCTGGTGCTGGTCA580.11238785436084252No Hit
TCAATCGGGACTAAATATAAATATATGAAAAAGATGTCTCACTTTATTACTG580.11238785436084252No Hit
AAGCTAGGGATTGTGATAGTGTCCAGCAACTGATACAGCAAAACTCCCTCCT580.11238785436084252No Hit
GATGTAGGGCACCAATTAAAAATACAAAATTCAACTTTATATCGCTTATAAG580.11238785436084252No Hit
GTGAATGGGAAGGGGCAGAAGTTGCTATTTAGATTTAGTAAGTGATCCAACT580.11238785436084252No Hit
AGCAGGGCAAGAATTTCTGCAGGTACTACTGCTATTCATAATATACTATAGC580.11238785436084252No Hit
AGCACAGGGACATACCTCTGCAGTACCTCACCAGGGAGCATTCCAGTATCAT570.11045013273393145No Hit
GAGTATGGGATTTCCTATGATGTATACTGCCACTAACCTTCCAAAAATTACT560.10851241110702037No Hit
TAACAGGGACATGTATGATTTGTGCCACTGATCTTAAACCTATGATTCAGTA560.10851241110702037No Hit
AATCTAGGGAGCATTTTTGTAGAGCTCTGGAAACTCAACATTAATTTTTAGT560.10851241110702037No Hit
ATGCCAGGGTAAAAATTTAGGTCTTCTAATATCTCCAACAACCCATCTTCAA560.10851241110702037No Hit
TGTGGCTGGGGGATTTTAAAAACAAGATCTAATTATATGTTGTTTGTAAGAG550.10657468948010929No Hit
ATTTGAGGGAACAAAGTAAATGTTCACCAACAGGAGACTGGATAAACAAAGT550.10657468948010929No Hit
TAACAAGGGGGAATCAACCCAAATGCCCATAAATGATAGACTGGATAAAGAA550.10657468948010929No Hit
CCAGACGGGATGCACTGGAAAGTCTCAACAACAGAATCAAACAAGTAGAAGA540.1046369678531982No Hit
TGCTTAGGGTCCAATTTCTTATACAGCCACAGAATATGTTTTGCACAACCAA540.1046369678531982No Hit
GATCACGGGCCTACTCAAATGGCCCGCTTGAAGAAAATATTATAATGTCTTC540.1046369678531982No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGT540.1046369678531982No Hit
AACAAAGGGGCTATGGTATAATACGCCTCACACTCATTCTCAACCCCCTGAC540.1046369678531982No Hit
CATGAAGGGACAAGCACTCTGAAATATTATTGAGTATTACATATTAAAGTAG540.1046369678531982No Hit
ATTATGGGGGAATCCAGATTGCTCCTTCCTCTGCAGGAGCAGAATTTTTCCA530.10269924622628712No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTAT530.10269924622628712No Hit
ATCACTGGCGGGATCTGGTTGCGGGATGAAACTGTAGTTAGGGATACATTTC530.10269924622628712No Hit
CGCTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA530.10269924622628712No Hit
TAACCTGGGTGACAAACCGAGACTCCGTCTCAAAAAAGAAATAAATAAATAA530.10269924622628712No Hit
CAAACAGGGACCAGGATAATAAAAGTGGCAATAACAGGCCTTGTCTGATTCC520.10076152459937607No Hit
GGGCACGGGACATTACAAAGATTTACTATTTCTAAGTGATTTATAATTTTAC520.10076152459937607No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)