FastQCFastQC Report
Sat 31 Dec 2022
SRR5847983.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847983.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50445
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGCCGTCT12212.4204579244721978No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGC11452.2697987907622164No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTC1990.39448904747745067No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCA1260.24977698483496877No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCCTGTCTCTTATAC1000.19823570224997522No Hit
ATTCATGGGGACTTCATTCATTATAATGATTTCTGTTCAGCATCTCCAGTAT860.1704827039349787No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCAACTCGTATGC830.16453563286747944No Hit
GCTACCGGGGAGAAGCAGTCAGAGGGATTCTTGCATTGTGATGAGTGCATAT820.16255327584497967No Hit
TCAATCGGGACTAAATATAAATATATGAAAAAGATGTCTCACTTTATTACTG810.16057091882247992No Hit
TCGCCTAGGGGCAGTATAGTGTCACCTCAACCTCAAATGATATGGCTCATTG790.15660620477748044No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGT780.15462384775498067No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCCTGTCTCT760.15065913370998116No Hit
CCATTAGGGATGGGAGAGATAAAAAGAATTTGAAAGAGTTAGTGGAGTATAG750.14867677668748142No Hit
GATCACGGGCCTACTCAAATGGCCCGCTTGAAGAAAATATTATAATGTCTTC750.14867677668748142No Hit
CCACCAGGGCCTACCAGAGAGTGGAGGGTGCAAAGAAGGAGGGGATCAGGAA730.1447120626424819No Hit
GGAACTGGGATCCCCATGCATTTGAGAACCTTTGCATAGACTGTACTGCAGC720.14272970561998216No Hit
AGGTGGGGGTTCAGGCTGGTCTTGAGCTCCTGGCGTCGGGTGGTCCTCTTGC680.13480027752998316No Hit
AAGCTAGGGATTGTGATAGTGTCCAGCAACTGATACAGCAAAACTCCCTCCT680.13480027752998316No Hit
TGTATCGGGATTGTTGTAAAATGAGGAATGTATGTGAATATTCAGATAACCG670.1328179205074834No Hit
ACCTGAGGGCCAACTGGATTATAAATAAATTGAAAATAACTCATTATACCAA670.1328179205074834No Hit
GCTCGTGGGGATATGATTTTTCTTCAGGGAACAGAGGTCATATTTAAAGTGG670.1328179205074834No Hit
CTTCTCGCGGGATGAATATCACTAATATAGATATAAACTTTTACTGTACTTG660.13083556348498365No Hit
TTGCGCAGGGCTCGCTCCGGGGGGAATCATGGTCATTCCATGGAGACCTCCC650.12885320646248388No Hit
CAACAAGGGATTGGCCTTACAGTGGGCCATTCATTTCTATAGGCAAAGAAAG650.12885320646248388No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCAT650.12885320646248388No Hit
CAAACAGGGACCAGGATAATAAAAGTGGCAATAACAGGCCTTGTCTGATTCC650.12885320646248388No Hit
AGCAACGGGGCCAGAGGCAGCTATTTCTATGGCTCCTTTTGCTGCTGAGGAC650.12885320646248388No Hit
GGCTCAGGGGCTGATACCAAAACCTGGCAGACACAACAACAAAAAAATACAA640.12687084943998414No Hit
ATTTGAGGGAACAAAGTAAATGTTCACCAACAGGAGACTGGATAAACAAAGT640.12687084943998414No Hit
AATGCCGGATTTGTATGATATTTTAAAATCTCCTAAATGCTGGGCAATGGCT630.12488849241748438No Hit
GCAGCAGGGATACCATGTTCTTCCTTTTGGCTTAGTTTTGCCATGCATCCTG620.12290613539498464No Hit
TGCGCAGGGCAAGGAACTCAAGCAACAACAAAAAATCAAATAACCCCGTTAA620.12290613539498464No Hit
CTTTAAGGGGAAGGGCACTGAAAATGCCCTGGAAGCAGGAGAGAGGGACTGG610.12092377837248489No Hit
GTAATTGGGTGCATCCAATTGCTTGAACCATTTAAGTAGAGTTGACTGATTT610.12092377837248489No Hit
AGCTGGGATCGCTCAAGAAATTACAAGGATTTTAAAAGCTCTGTGCCAGGAA610.12092377837248489No Hit
GGGCACGGGACATTACAAAGATTTACTATTTCTAAGTGATTTATAATTTTAC600.11894142134998512No Hit
GTCTATGGGATATATCACCCTGTCGACTATATTTATGGATTTATTACTGGGT590.11695906432748539No Hit
ATCACTGGCGGGATCTGGTTGCGGGATGAAACTGTAGTTAGGGATACATTTC590.11695906432748539No Hit
TCCGCCGGGTATCAACAGCAGAATAAAGAAAGATTACATATGTAAAAGTACC590.11695906432748539No Hit
GGACTAGGGAAATTGAATAAATTTGCTTATTAGTTTTAAAATATTTAAGATT590.11695906432748539No Hit
AGATAGTGGGATCTGCAGTGGAACTAAAGAGTATCAATCAAAGTGATGAAAA590.11695906432748539No Hit
TTGACCTGGGACCCTGACTCTGACCAGTCTTTCAGTTCTCCAAAAGCCCAAT590.11695906432748539No Hit
TAACAAGGGGGAATCAACCCAAATGCCCATAAATGATAGACTGGATAAAGAA590.11695906432748539No Hit
CTAGCTGCGGGGCCCTTCCTGGGCATCGTGGCTGAGGTGCTGGTGCTGGTCA570.11299435028248588No Hit
TTGCCGGGGACCGGAGGGCACTGAAGCCTTGCCACTTGCTCAAGTGGGGCTG570.11299435028248588No Hit
GAGCAGGGGCATGGAAAAGAATGAGGCAGAGGCAGTATTTGAAGAGATAATG570.11299435028248588No Hit
TAAATGGGAGCAACTTAAATCTGCCTTATATTTCATCTTTTTGCTTCCTCTT570.11299435028248588No Hit
CGGCACGGGAGGAGTAAAACCCTATGAATGTAGTGAATGTGGGAAAACCTTC570.11299435028248588No Hit
GTTGCAGGGTCTATTATTGGGATGGATTGAAAGATAATATTGTTAAAATGTC560.11101199325998612No Hit
AGTGGGGGAGGATTGGGTTCAGGCATGAATAACAAAATCCCGAAACAATGGT560.11101199325998612No Hit
CCAGCTGAGGAACAGCATGGGCAGTGCCAGCCCCACCTGCCAGGGGCCATGG560.11101199325998612No Hit
ACTGTTGGGGAACCAAGGATATTGGAGGCTAGTCAAAGGCTACAAGGACATC560.11101199325998612No Hit
CGCACTGGGCACACGTTACAGAAGCTACGCATGGATCTGATCCGTTTACAGC550.10902963623748638No Hit
CTGTTCACGGGATAGTCAAACTCAAACTTCTGAAAACCAAATACAACATAAA550.10902963623748638No Hit
TTTGCAGGGCAACATTTGGTAGATCTGCAGAACTCTGTGAACCAATATTTTC550.10902963623748638No Hit
TCATTGGGGGAACCATAGCAAAAGGATACTGCATCTCTCATTACTGTAGTGC550.10902963623748638No Hit
AGCCAGAGAGAAAGGTCGGGTTACCCACAAGGGGAAACCCATCAGACTAATA550.10902963623748638No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTC540.10704727921498662No Hit
CCAGACGGGATGCACTGGAAAGTCTCAACAACAGAATCAAACAAGTAGAAGA540.10704727921498662No Hit
TGTAAGGGCAAATTGAAGTCCCGGAAAAAGCAAAATTTTTTTAAACTTTTTA540.10704727921498662No Hit
GTCTATGGGCACAGGTTATGTCATGTCGACACATGTAGTGTTTTCTATTTGA540.10704727921498662No Hit
CATTTAGGGAAACCCCTGAAGGAAGGTGACCCTTTAAGAGAGGATCATCTTT540.10704727921498662No Hit
GAATTAGGGAAGTAGAGGTACTAGTGGAATATCCTTTACGTTGTCTGGTGTT540.10704727921498662No Hit
GGATAAAGGGCATCATAATTTTGCATCATGCATGCATGAAAACAATAACAAA530.10506492219248688No Hit
CGAGAGGGGGCCTCATCGGATGGTCAGTACAGTGGGGTCACCTGTTGTTTCT530.10506492219248688No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCACTGTCTCTTATACAC530.10506492219248688No Hit
TATGCAGGGACAAGAAGTTCGTTCTGGTCTACAAGAACTCTTCCATGCAAGA530.10506492219248688No Hit
GTGAATGGGAAGGGGCAGAAGTTGCTATTTAGATTTAGTAAGTGATCCAACT530.10506492219248688No Hit
TCTTATTGGGCATCAGTGGGAACTCAGTACTCTGTATGTATCCACAAAAGGG530.10506492219248688No Hit
GGTACTGGGCGATCTTAATCAGGGACGATAGATGTTATTTTATAAATTTCAA520.10308256516998711No Hit
ACGAATGGGAGATCTCAAACACTTTATTGAGTTTACTACCAACATTGTCATT520.10308256516998711No Hit
CTCCATGGGTATTCATTTAAATTCAGATTATGTTTTAATAATAGCAACTTGT520.10308256516998711No Hit
GCTGAAGGGACAGATTGGTACCCGAGAACTGGAAGAGATGGGAGCAAAATTT520.10308256516998711No Hit
AACTGTGGGATCCTACTTATGGACTCACTCCGAGGCCCTTCTCGGTCAAATG520.10308256516998711No Hit
GGGAAGGAACTCGTTTATTGATTTATTGAAGATCTTTGGCTGTTTTTGATAA520.10308256516998711No Hit
GTCACTGGGCAGCCTGTACTAATTTATCTTAATGAAATCTAGGAACTAAGAT520.10308256516998711No Hit
TCTCGTGGGATAAGCCAAATTTTGGCCATGGAGCTTGTGATACACTTGCCTT510.10110020814748737No Hit
ATGAGAGGGTGGTGGTGTTTGGGAGGGTGAGATAGAATTTTAGAAGCTTGAA510.10110020814748737No Hit
CGACAGGGATGATAGATAGATAGATTAGATAGATAGATAGATACATACATAC510.10110020814748737No Hit
AATCTAGGGAGCATTTTTGTAGAGCTCTGGAAACTCAACATTAATTTTTAGT510.10110020814748737No Hit
ACTCGCGGGATATTTTTGTGGAGAGAAAGACACCAAAGAGTACATACTATAT510.10110020814748737No Hit
TTTCAAGGGACCAAGGTATGTGGACCGAGTGACTGAATTCCTCCAGCAAAAG510.10110020814748737No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)