FastQCFastQC Report
Sat 31 Dec 2022
SRR5847964.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847964.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42282
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGCCGTCT9282.194787379972565No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGC8692.0552480961165505No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGT1580.37368147202119106No Hit
GTTAGGGGGATTCACCACACTATTCTTGTGATTTCAAGTAATAAAAATCAGA1260.29799914857386123No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTC1230.29090393075067406No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCA900.21285653469561516No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCCTGTCTCTTATAC770.18211059079513742No Hit
TTTGGTGGGCACCTGGGCAGCTTACACATGATGAGGACTGGTTCTCAATATC710.16792015514876307No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCCTGTCTCT700.16555508254103402No Hit
CTAAAGGGGAGAACAAGTGGTTATCCATATGAGGCAAATTAGATTGGATACC670.15845986471784684No Hit
AGCCTTGGGACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGC660.15609479211011779No Hit
GTTTCAGGGGGTGTTGGTTCAGAATGGCACTTTGAATAATGTAAACCAGTGA620.14663450167920156No Hit
ACAGGGAGCATACACTTTCTTTACACCTAATAACATAAAGCAAGTGAGCGAC610.1442694290714725No Hit
TTGTTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC600.14190435646374344No Hit
GTATTAGGGGGATAGGCTAAGCTGTATTGTAATAACAGCAACAAAAACAAAA600.14190435646374344No Hit
ATCTCTGGGGGAGAAAGGTGGCGGCGCTTTCGGAGGGAATAAAATGGAAGGA590.13953928385601438No Hit
TAAACCAGGGAAGCCAGGAAAACCGCAAAATGATGTTTGAGAGTTCCTGTTT590.13953928385601438No Hit
AGCGAAGGGTACCTGGTGAGCGTCCCTCTGCGAATGTTCCGCCTCTGGGCGT590.13953928385601438No Hit
AACAGTGGGGTATTCTAACACCTGTGATACTGGGCATACTTGAGGTTGGGGC580.1371742112482853No Hit
CGGCATGGGCTCTTTGTCAGCTGGAGTTGCGCGGGCTGACGCGCCACTATGT580.1371742112482853No Hit
CTGCTAGGGGAATTGGAAATCTTCCTCTCCTCTCTTCTTCACCACCTTTTAA580.1371742112482853No Hit
TAGTAAGAGAGAGAAAATGAAATGCTTATACATTATTGGTGAGAATGTAAAT570.13480913864055627No Hit
TAGGCTGGGAGATGTCCTGGGCGTTCATATGGTAAGTTCTTCTTTATGTTTC560.1324440660328272No Hit
TGCTAGGGGGTCCCTGTCCTCAACCCCTGGTCTGACACTTGCAGATCAGGCA560.1324440660328272No Hit
TGTTGCGGGCTCCTGAGTGACTGGGATTACAGGACGACATAGACAGCCTAAA550.13007899342509815No Hit
CATGGTGGGGGAGTCCTTTCAGCTTGTTCAATTTAGATTTCCATTTCTTTCT550.13007899342509815No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCG550.13007899342509815No Hit
TACACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA540.1277139208173691No Hit
ATGTTTGGGGTGGGCGGGCGGCGGCGGCTGCGCGCGGAGGCGGTGGAGGAGG540.1277139208173691No Hit
TTTAAGGGGGATTGTGATGCCCTCAGGAAGTCTGGTTTCACTCTTTCTGTCA540.1277139208173691No Hit
CGTTAAGGGGCGGAAACTTGGGTGCGGAAGTGTGACTACATCCACCCAACTT530.12534884820964004No Hit
ACTAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC520.12298377560191098No Hit
GCCACAGGGAGCTCCTTGATGTTGTGGACCAGGAACTTCCGGAAGCCACTGG520.12298377560191098No Hit
TCAGCTGGGGGTGATCGCGGGATAGTATATTTTAGTGAAATTTAGCATAATA520.12298377560191098No Hit
TTATCTGGGGTAATACAGTATTTATTAGATAAGCTCAGTGAAACTTTTCTGA520.12298377560191098No Hit
TGACTGCGGGCAATCCTGTAGACCTTGACATTCAGAAAGATGTGAGCTGCTT520.12298377560191098No Hit
CTCTAGCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG520.12298377560191098No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCT500.11825363038645287No Hit
CAAGTAGGGCTTTGTTTACAGGGCAGAAAAAATTGAGTAAAACACAGGTTCT500.11825363038645287No Hit
AATAGAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC500.11825363038645287No Hit
GGTATGGGGAACGCAACTAACCATCACCACTAAGGTCCATTCCTTACTAGAA500.11825363038645287No Hit
GTTTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA500.11825363038645287No Hit
AAGTCTGGGATGTGAAAACGGCCCACAGGCACAGGAGTAAAATAGACCTGAG490.11588855777872381No Hit
GAATTAGGGCAATATGAAGTGTGGAGAGCACCCCGTCATGTCATATTTCTTT490.11588855777872381No Hit
TCACAGGGGCAAACAGGATATCCCACACTTCTTAACATGGCATAAAAGTCCC480.11352348517099475No Hit
TGAAAAGGGGTGTTTATTAGTCCGAAATATTATTATAAGGTATTTAAAGAGG480.11352348517099475No Hit
GTAGCTGGGAAGAGAGCAGGAGTGACTATACTAGTATCAAACAAAATAGAGA480.11352348517099475No Hit
GACAGCGGGAGGTAGTCCCTGGCAAAAGGTGAAAGCAATCTGCATTTTTAAC480.11352348517099475No Hit
GCAAAAGGGCTCCTGCAGAGTCGGCTTAATCACCTCCCATACTGGATTTCAT470.1111584125632657No Hit
GTAGGAGGGAGTTACAGCCTGTTAGTAACATGAGGGGACATTTTGGTGAGAA470.1111584125632657No Hit
AATTAGGGGCAGAAGAATAAATGTTTGATATTCAGGCTGCTATAGTTCACAA460.10879333995553664No Hit
AGTAGTGGGCAAATCTAAATTCTAGCCCTGGCACCCTTTTCTCGATACCAGA460.10879333995553664No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCACTGTCTCTTATACAC460.10879333995553664No Hit
CTACTCGGGGGAATCAACCCTTGCTAAAGAACACATCAGTCTGTATTGGTTG460.10879333995553664No Hit
AGCGGTGGGCATTAAAAGCCAACAACCACAACAATAAAGAGCAACCATAAGG460.10879333995553664No Hit
TTTCTGGGGAAGATCCTGAGAACAAGATATACAAAACAAAGCAGTAACATAA450.10642826734780758No Hit
TTACAAGGGAATGCAAAATCAAAACCATGATGAGATATCATCTGCCAGTCAG450.10642826734780758No Hit
ACATAGGGGCACATAGTGATTACAAAGCTGTTACACTTTTTGTTTCTATCTC450.10642826734780758No Hit
CGACAGGGGAACTATAAATGATAAAATTAGTAGACAAAACCATTAAAACAGC440.10406319474007852No Hit
CTCTACGGGGACTGACTGCGTCGGGGTTGAGACTGGGTGGATGAGGCTCACC440.10406319474007852No Hit
CTGATTGGGATCATAAAGCAAACACAAGGGAATTTAAAATCATGTCATAATC440.10406319474007852No Hit
ACAACCGGGGTGGGAAAAAAAGAGATAAGAGGAGGAAGCTAATAAGAGACTG440.10406319474007852No Hit
GCGGATGGGCATTCTTTATTAACCCTCCTTGGAATTTTGAAAACCTCGATTA440.10406319474007852No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCAT440.10406319474007852No Hit
TTCGTAGGGCGGGGGGAACATTATCTGAAATGTACATAGTGGAATCCAGGCA440.10406319474007852No Hit
TTAAATGGGAGTTATCTCAAGGTATAACTACTTAAAAAACTTTCTTGAATAT440.10406319474007852No Hit
ACCTGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA430.10169812213234947No Hit
GTCTCCATGCTTAATTCTTACTTGAAAATTTCCCCGAAGATATTCCTTGCCT430.10169812213234947No Hit
AGCACAGGAAAGAGAAAACTTGTGTGTGACCTAAGCTGTCACCTTTGCCTAT430.10169812213234947No Hit
TTATCGGGGGAATCTGTAAATAATACAATGAGATGCTAAAAACAAGTTTGAA430.10169812213234947No Hit
TCTACAAGCGGCATGAGATCGAATACCTTTTTTTTTACAGTACCTTTTGCCT430.10169812213234947No Hit
GTAATGGGGGGAAGTTTAAAAAAATAACAAAACTTTGAATGCATTCCGGGTT430.10169812213234947No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)