FastQCFastQC Report
Sat 31 Dec 2022
SRR5847963.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847963.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43102
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGCCGTCT8932.0718296134750127No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGC8551.9836666511994803No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGT1550.3596120829659877No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTC1320.3062502900097443No Hit
CTAAAGGGGAGAACAAGTGGTTATCCATATGAGGCAAATTAGATTGGATACC990.22968771750730824No Hit
GTTAGGGGGATTCACCACACTATTCTTGTGATTTCAAGTAATAAAAATCAGA950.22040740568883116No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCCTGTCTCTTATAC800.18560623636954202No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCA770.1786460025056842No Hit
CTCTAGCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG720.16704561273258783No Hit
TTTGGTGGGCACCTGGGCAGCTTACACATGATGAGGACTGGTTCTCAATATC670.15544522295949145No Hit
AGCCTTGGGACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGC660.15312514500487215No Hit
TCACTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA650.15080506705025287No Hit
GCCACAGGGAGCTCCTTGATGTTGTGGACCAGGAACTTCCGGAAGCCACTGG650.15080506705025287No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCCTGTCTCT650.15080506705025287No Hit
CTGCTAGGGGAATTGGAAATCTTCCTCTCCTCTCTTCTTCACCACCTTTTAA650.15080506705025287No Hit
GTTTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA640.14848498909563362No Hit
CATGGTGGGGGAGTCCTTTCAGCTTGTTCAATTTAGATTTCCATTTCTTTCT620.14384483318639504No Hit
TGCTAGGGGGTCCCTGTCCTCAACCCCTGGTCTGACACTTGCAGATCAGGCA610.1415247552317758No Hit
CGGCATGGGCTCTTTGTCAGCTGGAGTTGCGCGGGCTGACGCGCCACTATGT610.1415247552317758No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCG600.13920467727715652No Hit
AGTTTAGGGGTGGACAGCCATTGCTGAATAAAACTTAATGACGCCATAGAAA590.13688459932253724No Hit
GTATTAGGGGGATAGGCTAAGCTGTATTGTAATAACAGCAACAAAAACAAAA590.13688459932253724No Hit
TAGGCTGGGAGATGTCCTGGGCGTTCATATGGTAAGTTCTTCTTTATGTTTC580.13456452136791794No Hit
TTGTTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC580.13456452136791794No Hit
GCTAGGGGGTATGAAAGACATACCAAAAGAAATACCACAGAAATATCATAAG570.1322444434132987No Hit
CGCACTGGGGTGAAGCTGAAGCAAAAATGATGGTTGCTAACAAACCAGATAA560.12992436545867941No Hit
ATCTCTGGGGGAGAAAGGTGGCGGCGCTTTCGGAGGGAATAAAATGGAAGGA560.12992436545867941No Hit
GTAGCTGGGAAGAGAGCAGGAGTGACTATACTAGTATCAAACAAAATAGAGA560.12992436545867941No Hit
ACAGGGAGCATACACTTTCTTTACACCTAATAACATAAAGCAAGTGAGCGAC560.12992436545867941No Hit
GTTTCAGGGGGTGTTGGTTCAGAATGGCACTTTGAATAATGTAAACCAGTGA560.12992436545867941No Hit
TCTAATGGGGACGCTCTTCCTATCTCGCGTAGGGTGTATAAACAAAGGGCAA550.12760428750406014No Hit
CTACTCGGGGGAATCAACCCTTGCTAAAGAACACATCAGTCTGTATTGGTTG540.12528420954944086No Hit
TGACTGCGGGCAATCCTGTAGACCTTGACATTCAGAAAGATGTGAGCTGCTT540.12528420954944086No Hit
TAGACTGGGTACCTGTATGTTTTGTTTTAGACGAGACCAAACTAAACAAAAA530.12296413159482159No Hit
GTCTTAGGGATGAGTATGCAGCCCAGACAGCCTCTTGATACAGCCTTGTGAG530.12296413159482159No Hit
TCAGCTGGGGGTGATCGCGGGATAGTATATTTTAGTGAAATTTAGCATAATA530.12296413159482159No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCAACTCGTATGC530.12296413159482159No Hit
TTTAAGGGGGATTGTGATGCCCTCAGGAAGTCTGGTTTCACTCTTTCTGTCA520.12064405364020231No Hit
ACAACCGGGGTGGGAAAAAAAGAGATAAGAGGAGGAAGCTAATAAGAGACTG520.12064405364020231No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCACTGTCTCTTATACAC520.12064405364020231No Hit
TTATCTGGGGTAATACAGTATTTATTAGATAAGCTCAGTGAAACTTTTCTGA520.12064405364020231No Hit
GGGGGCGGGGAAAAATATTCAGTTCATCTTAAGTGCACTTTGTTTTGACTTG520.12064405364020231No Hit
ACTAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC510.11832397568558303No Hit
GACAGCGGGAGGTAGTCCCTGGCAAAAGGTGAAAGCAATCTGCATTTTTAAC510.11832397568558303No Hit
TTGATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC510.11832397568558303No Hit
TTCGTAGGGCGGGGGGAACATTATCTGAAATGTACATAGTGGAATCCAGGCA510.11832397568558303No Hit
TTCCTGGGATACATGGAGGCTGGCATCTTCATGAGTGCCAATCGTGGTCCTG510.11832397568558303No Hit
TCTACAAGCGGCATGAGATCGAATACCTTTTTTTTTACAGTACCTTTTGCCT510.11832397568558303No Hit
TGTTGCGGGCTCCTGAGTGACTGGGATTACAGGACGACATAGACAGCCTAAA500.11600389773096377No Hit
AGCGAAGGGTACCTGGTGAGCGTCCCTCTGCGAATGTTCCGCCTCTGGGCGT500.11600389773096377No Hit
TACCGAGGGGCGAGAAGATCCGAAAGAAATACCCGGACCGGGTGCCGGTGAT500.11600389773096377No Hit
ACAGCAGGAGTCTCTGGAGCAGAAATTATTTCAACTAGAAAGCAAAAATAGG500.11600389773096377No Hit
CTTTCATCGGGTCTGCCCATTCCATGCTCAAAGCTGTTTCCGTGAGGAAAGG500.11600389773096377No Hit
CGTTAAGGGGCGGAAACTTGGGTGCGGAAGTGTGACTACATCCACCCAACTT490.11368381977634448No Hit
GGTATGGGGAACGCAACTAACCATCACCACTAAGGTCCATTCCTTACTAGAA490.11368381977634448No Hit
ACACACGGGTGCAGTTTAAAAAGTAAATATATATTCTATCGTATTCATAAAC490.11368381977634448No Hit
CACCTGGGGGGCAGTCAGGGTCCGCCAGCTCGGGGCCAGCGCATGGGGCTGC490.11368381977634448No Hit
GTCGGCGGGGATCTGCTTCGGGGCTACCATGTGGAATGCAGTAGCATCCCTA490.11368381977634448No Hit
AACTTGGGGGCTATCGGAACTGATAAATTCAATAAAATTGAAGAATACAAAA480.1113637418217252No Hit
CTTGGTTGGGGATCCAGCTGGAGAGGGAGTTCCAGCGCTGCTGCCGCCAGGG480.1113637418217252No Hit
ACCTGTGGGGATGTTCCTTCCTTCCATACCCCAGCATACCTTCTCCTTGCTT480.1113637418217252No Hit
CTGATTGGGATCATAAAGCAAACACAAGGGAATTTAAAATCATGTCATAATC480.1113637418217252No Hit
GCATAGGGGATTGAATTCACTCTTGACTGTACTTTCTTTTATATTCTTTTTT480.1113637418217252No Hit
GCTCTCGGGGATCCCAACTCAAACAAACTATAGAGAGAGGAATGGGGGCAGA480.1113637418217252No Hit
GTCCGAGGGGGGGCATCTTTCCTCAATTCCATACTGACTTTGATCTTGAGAA480.1113637418217252No Hit
ATTGTTGGGCAATCATGGTGGAAGGCACCTTTTCACAGAGCAGCAGGGGAGA470.10904366386710594No Hit
ATGTTTGGGGTGGGCGGGCGGCGGCGGCTGCGCGCGGAGGCGGTGGAGGAGG470.10904366386710594No Hit
GTCTTGGGGATGGCAGGAACCAGAAAATGGATCTATGTCAGGATACTTTGTG470.10904366386710594No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGT470.10904366386710594No Hit
GCGGATGGGCATTCTTTATTAACCCTCCTTGGAATTTTGAAAACCTCGATTA470.10904366386710594No Hit
TAGTAAGAGAGAGAAAATGAAATGCTTATACATTATTGGTGAGAATGTAAAT460.10672358591248667No Hit
TAAACCAGGGAAGCCAGGAAAACCGCAAAATGATGTTTGAGAGTTCCTGTTT460.10672358591248667No Hit
TTATCGGGGGAATCTGTAAATAATACAATGAGATGCTAAAAACAAGTTTGAA460.10672358591248667No Hit
TTACGTGGGATTCCAGGTTCTTAATTTACATGGGAAGACAATGCAGTAGTAA460.10672358591248667No Hit
TACACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA450.10440350795786738No Hit
TGTCAGGGGGAGTTCAAAATGGCGACGGCGGCGGCAGCGGCGGCGGTGATGG450.10440350795786738No Hit
ACATGCAAAAAAATAAAAATAAAAATGAAGATAGATCCCTGTCTTACATCAT450.10440350795786738No Hit
CACAGTGGGAATTTTAATTACCACTAATGGTTTATTGTATATCTAAGGCATA450.10440350795786738No Hit
CGACAGGGGAACTATAAATGATAAAATTAGTAGACAAAACCATTAAAACAGC440.1020834300032481No Hit
CCAGCCTGGGTGACAGAGCAAGACTGTCTCAAAAAAATTTAAAAATAAAGTT440.1020834300032481No Hit
TAGCTTGGGGCAGATACAGTATATTCTCATCAACTGGAAGGTTATTTTGCAA440.1020834300032481No Hit
ATGGTAGGGGGTTTGCATATAAAAGGTAGACAGCTGATATGTTTTCATGAAT440.1020834300032481No Hit
GAGATGGGGAATTGAATTATTTCCATACTGATTTGGAATTCTCTTTTTATCA440.1020834300032481No Hit
AGCGTAGGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCA440.1020834300032481No Hit
GAGCTGGGGCTGGGCTCTCTGCTATGTTCTGTTGGTCTGTGTGTCTGTTTTT440.1020834300032481No Hit
GTCTTCGGGTAGTTCAATCTTAAATGTAATTCAGTAGTTCAGTCCTAGAAAC440.1020834300032481No Hit
ACTTCTGGGCAAGGGAAATAATGCTCTTTAGCTTCATTGAAATTAAAATATT440.1020834300032481No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)