FastQCFastQC Report
Sat 31 Dec 2022
SRR5847962.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847962.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41458
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGCCGTCT8592.0719764581021756No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGTATGC8091.9513724733465194No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGT1480.35698779487674276No Hit
GTTAGGGGGATTCACCACACTATTCTTGTGATTTCAAGTAATAAAAATCAGA1200.2894495634135752No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTC1030.24844420859665203No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCA830.2002026146943895No Hit
CTAAAGGGGAGAACAAGTGGTTATCCATATGAGGCAAATTAGATTGGATACC820.19779053499927637No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCCTGTCTCTTATAC780.18814221621882388No Hit
TCACTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA700.16884557865791885No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCG620.14954894109701383No Hit
CTCTAGCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG620.14954894109701383No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCAACTCGTATGC610.14713686140190071No Hit
CTTGGTTGGGGATCCAGCTGGAGAGGGAGTTCCAGCGCTGCTGCCGCCAGGG600.1447247817067876No Hit
TTGTTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC600.1447247817067876No Hit
TTTGGTGGGCACCTGGGCAGCTTACACATGATGAGGACTGGTTCTCAATATC600.1447247817067876No Hit
ACTAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC590.14231270201167445No Hit
TTATCGGGGGAATCTGTAAATAATACAATGAGATGCTAAAAACAAGTTTGAA590.14231270201167445No Hit
GTTTCAGGGGGTGTTGGTTCAGAATGGCACTTTGAATAATGTAAACCAGTGA590.14231270201167445No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCCTGTCTCT590.14231270201167445No Hit
GACATGGGGATGCCCGACCCTGGCCTGATGCCCCTGCCTGACACTGCTGCAG580.13990062231656133No Hit
AGCGAAGGGTACCTGGTGAGCGTCCCTCTGCGAATGTTCCGCCTCTGGGCGT570.13748854262144822No Hit
TTTAAGGGGGATTGTGATGCCCTCAGGAAGTCTGGTTTCACTCTTTCTGTCA570.13748854262144822No Hit
AGCCTTGGGACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGC560.13507646292633507No Hit
GGTATGGGGAACGCAACTAACCATCACCACTAAGGTCCATTCCTTACTAGAA550.13266438323122196No Hit
ACACACGGGTGCAGTTTAAAAAGTAAATATATATTCTATCGTATTCATAAAC550.13266438323122196No Hit
GCCACAGGGAGCTCCTTGATGTTGTGGACCAGGAACTTCCGGAAGCCACTGG550.13266438323122196No Hit
CTACTCGGGGGAATCAACCCTTGCTAAAGAACACATCAGTCTGTATTGGTTG550.13266438323122196No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTCGT530.1278402238409957No Hit
TTTGTGGGGCTAGCCTGGTATATGACTATGTCTGTTCTTGGACACTATAACA530.1278402238409957No Hit
TGACTGCGGGCAATCCTGTAGACCTTGACATTCAGAAAGATGTGAGCTGCTT530.1278402238409957No Hit
TCCTGAGGGATGGCTCCCGGACTGTGCAGGGAGCCGATACGTTTGCTGATAG530.1278402238409957No Hit
AAGACGGGGACACCCAAAGGAGAAGCTTGGTCTTTTCCAGGTATTTCCAACT530.1278402238409957No Hit
CGACAGGGGAACTATAAATGATAAAATTAGTAGACAAAACCATTAAAACAGC520.12542814414588258No Hit
TAGTAAGAGAGAGAAAATGAAATGCTTATACATTATTGGTGAGAATGTAAAT520.12542814414588258No Hit
GGTACGGGGGGGGCTGGCCGCCTCAGTGATGGTGATGCCACCAGCTCCAGCC520.12542814414588258No Hit
GTATTAGGGGGATAGGCTAAGCTGTATTGTAATAACAGCAACAAAAACAAAA520.12542814414588258No Hit
ACAGGGAGCATACACTTTCTTTACACCTAATAACATAAAGCAAGTGAGCGAC510.12301606445076944No Hit
CATGGTGGGGGAGTCCTTTCAGCTTGTTCAATTTAGATTTCCATTTCTTTCT500.12060398475565633No Hit
TGCTAGGGGGTCCCTGTCCTCAACCCCTGGTCTGACACTTGCAGATCAGGCA500.12060398475565633No Hit
GTAGCTGGGAAGAGAGCAGGAGTGACTATACTAGTATCAAACAAAATAGAGA500.12060398475565633No Hit
GCTAGGGGGTATGAAAGACATACCAAAAGAAATACCACAGAAATATCATAAG490.1181919050605432No Hit
CCAGCCTAGGTGACAGAGTAAGATCGTGTCTCAAAACAAAACAAAACAAAAC490.1181919050605432No Hit
TTGTTGGGGGAACTTTCAAACATTTGGTAAATATTATAGTATTGTAGTTCAG480.11577982536543008No Hit
TAGCTTGGGGCAGATACAGTATATTCTCATCAACTGGAAGGTTATTTTGCAA470.11336774567031695No Hit
TCTAATGGGGACGCTCTTCCTATCTCGCGTAGGGTGTATAAACAAAGGGCAA470.11336774567031695No Hit
ATTACAGGGGTCCGCTGCGTGCCGGACCATGGCGCAGGGGCAGCGTAAGTTT470.11336774567031695No Hit
TTGGTAGGGAGTGAGCAGTATGACTTGTACATGCCATTGAAAATTATTAATC470.11336774567031695No Hit
TAAGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC470.11336774567031695No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCT460.11095566597520382No Hit
GTTTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA460.11095566597520382No Hit
TGAAACGGGAAGCTGTTAAAAGGTTTTATATTTTAAAATTACCTTCTTGATA460.11095566597520382No Hit
TAGGCTGGGAGATGTCCTGGGCGTTCATATGGTAAGTTCTTCTTTATGTTTC460.11095566597520382No Hit
TCCCGAGGGCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGA460.11095566597520382No Hit
ACTTCTGGGGAGTGAACCCTGAAGAATATAGCAGGAGATGCGAAGATGTGAG460.11095566597520382No Hit
ACGTTAGGAACTCTGGCCATAACACAACAAATAAACACAGGAAGAATCTGAA460.11095566597520382No Hit
TAACTCGGGGTTCTAATCTTGGCAACAACCATACAGTTTATATATATGGGTT460.11095566597520382No Hit
TAAACCAGGGAAGCCAGGAAAACCGCAAAATGATGTTTGAGAGTTCCTGTTT450.1085435862800907No Hit
TGGTAAGGGATATATGACTCAATAGAAAAATGAGGAGAAGCTGATAGGTACA450.1085435862800907No Hit
GCTCTGGGATTACAGGCGTGAGCCACTGCGCCCAGCCTATACTTTATTTTTA450.1085435862800907No Hit
ATGTTTGGGGTGGGCGGGCGGCGGCGGCTGCGCGCGGAGGCGGTGGAGGAGG440.10613150658497757No Hit
CGTTAAGGGGCGGAAACTTGGGTGCGGAAGTGTGACTACATCCACCCAACTT440.10613150658497757No Hit
GTCTCCATGCTTAATTCTTACTTGAAAATTTCCCCGAAGATATTCCTTGCCT440.10613150658497757No Hit
TTTCTGGGGAAGATCCTGAGAACAAGATATACAAAACAAAGCAGTAACATAA440.10613150658497757No Hit
GTCTTAGGGATGAGTATGCAGCCCAGACAGCCTCTTGATACAGCCTTGTGAG440.10613150658497757No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCGTCACTGTCTCTTATACAC440.10613150658497757No Hit
AAGCACGGGAAGGTAAGTTTGGGAGCATATGAAATTGTATGCAAGACTCTTA440.10613150658497757No Hit
ACAGCAGGAGTCTCTGGAGCAGAAATTATTTCAACTAGAAAGCAAAAATAGG440.10613150658497757No Hit
CGCACTGGGGTGAAGCTGAAGCAAAAATGATGGTTGCTAACAAACCAGATAA430.10371942688986444No Hit
TACACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA430.10371942688986444No Hit
GACAATGGGGGAAGGAGCCCGAATCACTGATGGAATTGGACAGTGCATGGAG430.10371942688986444No Hit
CGGCATGGGCTCTTTGTCAGCTGGAGTTGCGCGGGCTGACGCGCCACTATGT430.10371942688986444No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCAT430.10371942688986444No Hit
GTGTGGGGAAAGTGACAAAGACTCTTAACGTAGGATATATGTTCTGTTCAAA430.10371942688986444No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACATTGCGTCATCTC420.10130734719475132No Hit
ATCTCTGGGGGAGAAAGGTGGCGGCGCTTTCGGAGGGAATAAAATGGAAGGA420.10130734719475132No Hit
TTACCTGGGTGAACCATAACCAATACTACCAATCAATACTCATCATTAATAA420.10130734719475132No Hit
GCCAATGGGATGGCCACATAATTCTTAATGTCAGCTAGTATAATGGTTTACA420.10130734719475132No Hit
ATTTGGGGACACAGAGATACGCGCACACACACACACAAACGCACTCAGATTT420.10130734719475132No Hit
GAGCCAGGGACAGGGAGGAAAAGTAACAATAGTCCACATGTCCCTGGCATCT420.10130734719475132No Hit
ATGCATGGGGATATAGAACCCTGTATCTTATCTCAGTTCTTTTATGTTATCT420.10130734719475132No Hit
TCAGCTGGGGGTGATCGCGGGATAGTATATTTTAGTGAAATTTAGCATAATA420.10130734719475132No Hit
TTCGTGGGGGAAAGAAGCCTTTCTTTGAGTGACACCAAAGGAAAAAAACAGA420.10130734719475132No Hit
GATAGTGGGACCTGTGAACCTCTTCCCTCTATACTAAGGCAGTAAGAAATAT420.10130734719475132No Hit
GTCGGCGGGGATCTGCTTCGGGGCTACCATGTGGAATGCAGTAGCATCCCTA420.10130734719475132No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)