FastQCFastQC Report
Sat 31 Dec 2022
SRR5847949.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847949.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165663
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGC4090.24688675202066845No Hit
CTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGCCGTCT3470.20946137640873336TruSeq Adapter, Index 3 (95% over 22bp)
GTACACGGGCTATCTATTAAGAACACTATGGAATTTCTATTCAAATTGGGTA2300.13883607081846883No Hit
GGCAAAGATTTCATGACAAAGATGCCAAAAGCAACTTCAACAGAAAAAAAGT2170.13098881464177276No Hit
AAGCGAGGGCTTCTATATTTTCTTAAAGCTGGTAATAGACTATAAAAACTAA2100.1267633690081672No Hit
GCTAATGGGATTCCGAGTAAAAGTACAACATAAAGACAACCATGAAGGAATG2050.12374519355559177No Hit
ATCTCAGGGATGCCAAGATTTTGGGGTTTTTTTGTTTCCTTTTTTTGTTTGT2000.12072701810301636No Hit
GATTTTGGGTGATCCCCAGTGACCACGCCCCTCTGGCCCAGGGTTCCAAGGA1970.11891611283147113No Hit
ATGCATTGGGGGTTTTGATTTGCATTTCTCTGATGGCCAGTGATGATGAGCA1930.11650157246941079No Hit
TCGCATGGGAAGTAGCCCCGAGCGGGAGGCTGTGGCGGAAGTGGTCGCGTTA1870.1128797619263203No Hit
AAGGTAGGGAGGAATCATATTGCTTTTGTGAAACAAAGAAAATTTACCACCT1860.11227612683580522No Hit
GCAAATGGGGATTATAGCAAGTTTGCAGGACACGAGGTTAATATACAAAAGT1860.11227612683580522No Hit
GGCCATGGGAGACTGAAAATTTGAATTCACTGTTAAGAGGAGACATGGAACT1850.11167249174529013No Hit
TGAAAAGGGGAATGCATTGAATAAACTGTCTTTTGAGGTGAAATTTTGGCAT1850.11167249174529013No Hit
AGTAGCGGGATTCAGCCATGCAACAGTACCAGACCTTCTGTTAGTGCTGTGT1840.11106885665477506No Hit
CGCTAGGGGCGTTTGCTTATTAGTAAACTTTATCCAGGAGAAAGTATTGGTC1840.11106885665477506No Hit
TTGCAAGGGGGGGGCTATTTAAGAGACAGCCGCCCGCTGGTCCTCCCTGAAC1830.11046522156425997No Hit
TTCATAGGGAGAGTGTGGTCAGGCGGCTCGGACTGAGCAGGACTTTCCTTAT1800.10865431629271471No Hit
CGTTTGGGGAGTGCAAAATACACAGTGAATTTTGAAGTCTTAGTATGAAAAA1800.10865431629271471No Hit
AGCAACGCGGGACTCGCTGATATGAATCCCAGAAAAAAATTTCCTGTTTTTA1790.10805068120219964No Hit
GATTCAGGGATTGCAAGAGGCGAGTTTAAAATTCTGCAGATGGCCTTATTTG1790.10805068120219964No Hit
CACTACGGGATAAGGTAAAATTTTCCTGTTTTTCCCCCTTTGAAAAACTCAG1780.10744704611168455No Hit
GAGCCTGGGGTGACCATTGCTTTTCTGATGCCACGTACTCTAGAATCTGAAA1780.10744704611168455No Hit
CCCAGGGGATTGAACAAACTTACATGGCTATCATATGTGTCAGAGACTGCTC1760.1062397759306544No Hit
GTGCGCGGGTATGAGTATCTGTGTGATTCTCATAGATAATTAGTCAATAGAA1760.1062397759306544No Hit
TAAGCTGGGCTTTGATTTCCTATCACACCTAGCAAAAATACCTTGTACTTGG1760.1062397759306544No Hit
GGCTCCCTACCTTGGCTGACAAATTCTCACTTTGGGACTTAGTCATTGTTGC1750.10563614084013932No Hit
ATGCTCGGGAACCCCAAACTCTAGTTAAATTGCTTCTCGGAGGAAAATCTTA1750.10563614084013932No Hit
TCAGCAGGGCACTGCAGTAATGTCAATGGTTGGTTATATTCTGGGGCTTGGA1750.10563614084013932No Hit
TATAAAGGGGCCTATATAAGCCATGCGCAGGCGGAGGAGCGCCTCTTTCCCT1750.10563614084013932No Hit
GCTCAAGGGCTTAAAAGCAGTACAGAAACATACACACACATACAAATACAAG1730.10442887065910916No Hit
CGTAGCGGGATTTCCCTCAGGAACAAGGGTTTACTAATTCATTGTCCACTGT1730.10442887065910916No Hit
CAAGCTGGGAATTGAAAGCAAGTGAAATATTCAAATAAACATATACGTATTT1730.10442887065910916No Hit
GAGATCACGCCACTGTACTCCTGCCTCGGTGACAGAGTGAGACTCCATCTCA1730.10442887065910916No Hit
TTATACGGGGGCACTGGTAGATTCTCATGTACATGTATGAGTCTGAAATTCA1730.10442887065910916No Hit
GTCAAAGGGTAGCTAGTCTCAGCCAAGTAAGTTTGTGATGAAAGGATATGAG1720.10382523556859408No Hit
TTGTTTGGGGAAGAGCAGTTGCTGATCACGATGGGAAAAGAACCTACTCTCC1710.103221600478079No Hit
CTTTACGGGATGAATAAGCTAGTCATGGCTAGGATAATCCATTTTCATGTAT1710.103221600478079No Hit
CCAGTCGGGGAGTGGTTGATGACGAACTGGCAACCAGGTTTCTTAAAAGTTT1710.103221600478079No Hit
CACTTTCGGGAAGTTGACAATGTGGTGAAATTTCAAAATTATATGTAACTTC1710.103221600478079No Hit
TTCTTAGGGGCGCTGAGAACGCGGGTCCACGCGTGTGATCGTCCGTGCGTCT1690.10201433029704884No Hit
GCTCAGGGAGCTTCAGTGACCCAGGGTCTGAACTGCCTCCATCCTAGGGCAA1680.10141069520653374No Hit
TCGTAGGGCAAGGCTATTTTTGAAAAGTTGCACTTTTGCCATTTTCCTTAAG1680.10141069520653374No Hit
GCTACGGGAGGGCTCAATTGGCAGTCTCAGGAGCTTGGGATGCTACCAACAG1680.10141069520653374No Hit
ACTAAGGGAAGATTTGCTGTTAGAAATAACTCTTGTGAGTTTTATATTGTGC1680.10141069520653374No Hit
AACGTCGGGAAGTACTTATAAAATGAATGCTCTAGTACTTCTGTGGAATATA1680.10141069520653374No Hit
CAAACTGGGTATTATTTTTGAGACAGAGTCTTGCTCTGTCGCCCAGGCTGGA1680.10141069520653374No Hit
ATCTGGGGGTTGGGAGGAGTGAAGGGGAGGCTGTGTCCCATCACAGGGAAGG1670.10080706011601867No Hit
GCTTAAGGGGAGGCCACACCCTTCAAGTGGTTCATGGAGATGTGTGTCCGAG1670.10080706011601867No Hit
GTAAGTGGGTCTTGTGTATCATGATGTTCAACTTTGCTTTTGCTGTGCCAGT1660.10020342502550358No Hit
TTCACAGGGAAGAAAACAAGAGAAAAAGCTTCTGCTACTAGAGTGTATATTG1660.10020342502550358No Hit
CAAAGTGGGTACTTTCATCTAAAATTACTACCTGCACTGAAATGTATAGATT1660.10020342502550358No Hit
AAAAAAGGGCTTCATTGTTTGTTATTCATAGCCAGAAGTTTTCTACAGATTT1660.10020342502550358No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)