FastQCFastQC Report
Sat 31 Dec 2022
SRR5847947.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847947.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161516
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGC3650.22598380346219568No Hit
CTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGCCGTCT3620.2241264023378489TruSeq Adapter, Index 3 (95% over 22bp)
GGCAAAGATTTCATGACAAAGATGCCAAAAGCAACTTCAACAGAAAAAAAGT2190.13559028207731744No Hit
GTACACGGGCTATCTATTAAGAACACTATGGAATTTCTATTCAAATTGGGTA2190.13559028207731744No Hit
GCTACGGGAGGGCTCAATTGGCAGTCTCAGGAGCTTGGGATGCTACCAACAG1860.11515886970950245No Hit
GAGCCTGGGGTGACCATTGCTTTTCTGATGCCACGTACTCTAGAATCTGAAA1850.11453973600138685No Hit
ACATCAGGGGAATCCGGAGTGCCCAACATGGCGGCGGCCGTAAGGTGCATGG1840.11392060229327126No Hit
TGGCCAGGGTGTGCAGGGTGAGAGAAAATATTTGTACTACAAATATCTGTCC1840.11392060229327126No Hit
CACGAGGGACTTAAACTTTGGTAGGAAACAAACTAAAGCAGACAAACATTTC1820.11268233487704005No Hit
GCTGGCGGGGTTTGGGAACAGTACCTAACTCATAGAGTCATTGCAAAAATTA1810.11206320116892445No Hit
TTGCAAGGGGGGGGCTATTTAAGAGACAGCCGCCCGCTGGTCCTCCCTGAAC1800.11144406746080883No Hit
AGTAGCGGGATTCAGCCATGCAACAGTACCAGACCTTCTGTTAGTGCTGTGT1800.11144406746080883No Hit
GCTGGGGAACTCAGCAGGTCTTGTTTGCCCAGACTCTGTACATTTCCAAGAA1790.11082493375269324No Hit
TCGCATGGGAAGTAGCCCCGAGCGGGAGGCTGTGGCGGAAGTGGTCGCGTTA1790.11082493375269324No Hit
GCTCAGGGAGCTTCAGTGACCCAGGGTCTGAACTGCCTCCATCCTAGGGCAA1780.11020580004457761No Hit
AGCAACGCGGGACTCGCTGATATGAATCCCAGAAAAAAATTTCCTGTTTTTA1760.10896753262834642No Hit
GATTTTGGGTGATCCCCAGTGACCACGCCCCTCTGGCCCAGGGTTCCAAGGA1760.10896753262834642No Hit
TTCGCAGGGGCTATTGGTTAATTGTTGTGGGCATGTTTTTTTTAACATGTAA1750.1083483989202308No Hit
AAGGTAGGGAGGAATCATATTGCTTTTGTGAAACAAAGAAAATTTACCACCT1750.1083483989202308No Hit
CTTTACGGGATGAATAAGCTAGTCATGGCTAGGATAATCCATTTTCATGTAT1750.1083483989202308No Hit
ATCTCAGGGATGCCAAGATTTTGGGGTTTTTTTGTTTCCTTTTTTTGTTTGT1750.1083483989202308No Hit
GTGCGCGGGTATGAGTATCTGTGTGATTCTCATAGATAATTAGTCAATAGAA1750.1083483989202308No Hit
TATAAAGGGGCCTATATAAGCCATGCGCAGGCGGAGGAGCGCCTCTTTCCCT1750.1083483989202308No Hit
AAGCGAGGGCTTCTATATTTTCTTAAAGCTGGTAATAGACTATAAAAACTAA1720.106490997795884No Hit
GGCCATGGGAGACTGAAAATTTGAATTCACTGTTAAGAGGAGACATGGAACT1720.106490997795884No Hit
TTCCTTGGGCAACCTCAGCTCACTGCAACCTCCATCTCTGGGGTTCAAGTGA1720.106490997795884No Hit
GCTTTGGGTAATAATGGATTAATAAAGATGAATTAATTATATATTACTTAAC1710.1058718640877684No Hit
TATGCCGGGGAGTGGACCCCTTCTAACTATAAATTTTGTATTTTCAACATTT1710.1058718640877684No Hit
CCAGTCGGGGAGTGGTTGATGACGAACTGGCAACCAGGTTTCTTAAAAGTTT1710.1058718640877684No Hit
ATGACTGGGGATCTTAGCTTTAAGACTGAGACTTTCATTTTAAAAATAAAAT1710.1058718640877684No Hit
CACTTTCGGGAAGTTGACAATGTGGTGAAATTTCAAAATTATATGTAACTTC1700.10525273037965278No Hit
ATCTGGGGGTTGGGAGGAGTGAAGGGGAGGCTGTGTCCCATCACAGGGAAGG1680.10401446296342158No Hit
CGTAGCGGGATTTCCCTCAGGAACAAGGGTTTACTAATTCATTGTCCACTGT1660.10277619554719038No Hit
CACTACGGGATAAGGTAAAATTTTCCTGTTTTTCCCCCTTTGAAAAACTCAG1660.10277619554719038No Hit
ACTGCCGGGGTCTCAAATTCCTGTCCTCAAGTGATCCTCCTGCCTCTGCCTC1660.10277619554719038No Hit
TGTTCTGGGAATGAGACTTTGACATTCCTTGGCGAAGAAGATGTCTCCTGCC1650.10215706183907478No Hit
CCAGATTGGGGACCTCGGCCTCACAAGGTGCTGAGGTTACACGTGTGGGCCA1650.10215706183907478No Hit
GTAGGGGGGACAATCTCAAATTTCCTGCTGCTTCTCCTTAGAAATGAAAATG1650.10215706183907478No Hit
GCTAATGGGATTCCGAGTAAAAGTACAACATAAAGACAACCATGAAGGAATG1640.10153792813095916No Hit
CATTAAGGACGCCCTGGCGGTAATTATGGGCATTCTGACTTTGGTTGGAGGA1640.10153792813095916No Hit
CTCTGTGGGATAATTTTTCAAGTAAACAAATTGTTTCTCTAAGTTGAAAATT1640.10153792813095916No Hit
TTCTTAGGGGCGCTGAGAACGCGGGTCCACGCGTGTGATCGTCCGTGCGTCT1630.10091879442284356No Hit
CTCCCCATGGGTGCCGCCTCGCAGAGATGCCATGCCCTCTGACGCCAACCTT1630.10091879442284356No Hit
CAAACTGGGTATTATTTTTGAGACAGAGTCTTGCTCTGTCGCCCAGGCTGGA1630.10091879442284356No Hit
GGCATAGGGAGAAACTCTTGAAGCCTATGTTGACCACTGCCTTGGCTTAATT1620.10029966071472794No Hit
AAAAATGGGATGCTCATTGGGTTGTGTCTGTCACCGACCCTCCCTGCTGTCA1620.10029966071472794No Hit
TTCACAGGGAAGAAAACAAGAGAAAAAGCTTCTGCTACTAGAGTGTATATTG1620.10029966071472794No Hit
TCAGCAGGGCACTGCAGTAATGTCAATGGTTGGTTATATTCTGGGGCTTGGA1620.10029966071472794No Hit
GACCATGGGGCGCCGGGGGGAAGATGCTTTTATAGCTCTTTGTGATGTATTA1620.10029966071472794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)