FastQCFastQC Report
Sat 31 Dec 2022
SRR5847943.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847943.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163059
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGC6090.37348444428090444No Hit
CTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGCCGTCT6090.37348444428090444TruSeq Adapter, Index 3 (95% over 22bp)
CAAACAGGGGAGTTCATCCATCAATTTCCTGACTCGAGTTAGTGGCATTGGT2120.1300142893063247No Hit
GCTAATGGGAGTTTTTCTTAAGCTTTTGGACTGGTAAGTGGCATTTATGAAA2020.12388153981074335No Hit
GTGCTGAGGGGCCCTTCCTCCCTCCCTCACACGCCTCCACCTCTTCCCGATC2010.12326826486118521No Hit
AGCTTCGGGCGCTGGCTCCTGTTTCCAATTGATTGAGGGATGCCCCCAAGGA1980.12142844001251081No Hit
TAGTCGGGGGAGGAGCAGAGATGAGAGTAGAATTTGTGTTTTCTGCCTTTTT1960.12020189011339455No Hit
ACTTACGGGACCACCAGCCCACCAGATAAAGGCCCCCCAAAGAGTAAACTAA1930.11836206526472012No Hit
ATTAAAGGGTGCCCTGCAGGGTGCTCTCCAGCAGTGGCTGGGGCCTGCGTGG1900.11652224041604572No Hit
GATAGAGGGTGCGAGGGGAACTGGAACAAACAAGATTAGAAGCTGAATTTTC1900.11652224041604572No Hit
CAAGGTGGGCACTTTATGCACCAAAGTGAAAGAATTCAGTGTATCCGTTATT1860.11406914061781318No Hit
CAACCTGGGAAACTTCACTGAGAGCACAGATGGAGGAAAGGCCATTTTAAAA1850.11345586566825505No Hit
TAGTTTGGGGCTCGTGGGCTGGTGACTGGGTCTTGGGATCCCAGGCCACGCG1850.11345586566825505No Hit
GACATGGGGCCATACCTGCAAGTTCTGTGACTTTGGCGAGGGTGATGGAGGT1840.11284259071869691No Hit
CCAAGAGGGAGGTTGCTCTAAGCAAAGTCCACGTTTTTTTAATTGAATTCTG1830.11222931576913879No Hit
AACCAGGGGGGACTTTTGACAAACAGATCGTCTTCGTTCTGAATCCTCAGTG1830.11222931576913879No Hit
CAATCCGGGTCTACAAAAGGGAGAGGTAGCTTTTTTAAAAAAGAAGAATCTA1830.11222931576913879No Hit
CTTTGTGGGAAGATTTTGCCAAGTATGTGTTGTTGCTTGACTTCTTAAATAT1820.11161604081958065No Hit
TACGCTGGGGTGACTTAATGTTTGATTAGGAAATATTTTGTTTGGTGTAAGG1820.11161604081958065No Hit
TTAAAAGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGCG1810.1110027658700225No Hit
TTCTCTGGGGGGAACGGGAGAGAGAAAGAGAAAAAGAGAGAGATCGTGAAAG1810.1110027658700225No Hit
CATCACGGGGATGTATGTGTTAATTTAATTTGTTAAAAACCAAAAAGCAAAA1810.1110027658700225No Hit
GTAATGGGAATTTGTTCATGGTTATTCTACATTTCTAGAAAGTTCTTATCAG1800.11038949092046436No Hit
TTAGGTGGGGAGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCCGG1790.10977621597090623No Hit
GCTTGCGGGAGCTTGACCAGTATAAAATTACAGTGGATTGCTCATCTCAGTC1790.10977621597090623No Hit
TTGAGGGGGCAATGGGGAAGGGACACCATATTCAATAAATAGGGCTGAGATA1780.1091629410213481No Hit
TGAAGTGGGGATAAGGCCACGTTTCATCTCCTGTTACAATTCTTCAAAGAAA1770.10854966607178997No Hit
TGTAATGGGATAGTAATACATCTAACCAAAGAGGAGAAAGATCTCTACAAGG1770.10854966607178997No Hit
GAACGGGAGCAGGTTTGAAGGGAGGATCTTCTTCAGTTGTAGTTTGGAAGGT1750.1073231161726737No Hit
TAAGCGGGGCTACACAATTTGGTCAAAACCTGCCAGTGCAGCCTCTTCTTAC1740.10670984122311555No Hit
TGTAACGGGTTATTGGATTTGCACCTAACATAGATCATAATTTTTGTATCTT1740.10670984122311555No Hit
CTGTTTGGGATTGCTTGAGCACAGGAGGTTGAGACTGCAGTGAGTCAAGATC1740.10670984122311555No Hit
GCAAATGGGGATGCTAAATATTTTCCTTAGGAAGTTTTGTTTAATGTTAGAT1740.10670984122311555No Hit
GACAGGGGAGGGAGTCGAGGGCAGAGGCAGCAGGCGGAGAGAGGGAGATAGG1730.10609656627355742No Hit
GCATCCCGGGATATTGTTTTTTGAATAATTAGTGATATTTCCCAGTAAGTTC1730.10609656627355742No Hit
TTGGCTGAGCTGAGACACCTCCTGTTTCCCTTCCCCTTGAACCAGTCCCAGT1730.10609656627355742No Hit
GGCTTAGGGTGTGATAAAATCAGGCTAATTTACTTGGATTTGGGGTGATTTG1730.10609656627355742No Hit
ATCCAGGGGCAGGCACAGAGGAACGAACTAAATGCTAAAGTTCGCCTATTGC1730.10609656627355742No Hit
TATTGGGGAATACCTGAAAACTGTAGCTTCATATCTTTTATGTGGTTTTCTA1730.10609656627355742No Hit
TCTCAGGGGATCTCGGCTCACTGCAAGCTCTGCCTCCTAGGTTCATGCCATT1730.10609656627355742No Hit
TCATAAGGGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAAC1720.10548329132399929No Hit
TAGTTAGGGCTTACAGATGTCAGGTAATTTGTTTCAAAAATTTGTGAGTTCA1720.10548329132399929No Hit
ATCGCTGGGATATGTGGTTTTACACATGATGATGGATACATGATCTGTTGTG1720.10548329132399929No Hit
TACAGCAGGGGCTGCTATGGAGGCGCTGGCCATGGGTTCCCGGGCGCTGCGG1720.10548329132399929No Hit
CTGCCTGCCAGCAGTGCCTTGCATTTGTGATCCTACAAAGAGCCTGTGGACT1720.10548329132399929No Hit
AGATAAGGGTCGATATTTCAGATGGGCTTTTGATGCACTGTTGCCAAGGAAG1720.10548329132399929No Hit
ATAACTGGGAAACAAGGCATGCCACACCATTGCTGTTCCCCATCTGGTACTA1720.10548329132399929No Hit
CTCGACTGGGTATCTCTGTAATGAGAAGTAATTAGCCCCAAACTGACAACTG1710.10487001637444116No Hit
TTTCTGGGGACTTGCCGCAGTACTCTTAAAACTAGGCGGCTATGGTATAATA1700.10425674142488302No Hit
GTGGGTGGGGAACCGAGTCCTGGTCAACCTGTTCCTGCTCATCTCCTCCATC1700.10425674142488302No Hit
GAGACGGGGCAGACCCAAATATTATATTCATTTTAAAAATATTTTTCTTTTG1700.10425674142488302No Hit
CAGCGGGGCAAGAAAAATGAGGGAATGGAAACTATGGGAGCCATTAGTGGAA1700.10425674142488302No Hit
TGCAAAGGGGCATCCTCATAGCTCAGATCTCCTTTCAAAGTAGTGGCTTTCT1690.1036434664753249No Hit
GCTTAGGGGAAAGTGAGCATCCTTGTCCTGTTCCTGATCTTAGAGGAGAGAC1690.1036434664753249No Hit
TAACACAGGGAGGAACTGTAAGCTTTTATCTTTTAACCAACTGAACAATACA1690.1036434664753249No Hit
TGCTCCGGGTCCTTAACTACATATGCTAAGCCCTTTCGTGCTTCCAGCTTAA1680.10303019152576676No Hit
GCAAGGGGCAGTTAGTGAAAATGCCATAAAGCTCACTGTTCTTGCCAAGATG1680.10303019152576676No Hit
TCAACAGGGCTCTTCGTATCCTAGAAACTCTTCAACTTGCTGCCTCTGATTT1680.10303019152576676No Hit
CCAGTCATGGAGTTAAGAAGTGAGTCAGAGAGTAGATGGGTAGGGACAGTGA1670.1024169165762086No Hit
AAGAAGGGGAAGCACTGAGATTGCAGGCATGAGTCACTGTGCCCAGTATTTT1670.1024169165762086No Hit
GCAGCTGGGGGGAGAACAATATGGCGGATGGCGAGGAGCCGGAGAAGAAAAG1660.10180364162665047No Hit
GGCATGGATGCATAAAAGTGTTTCAAAAACTTCCTAGCACAAAGATAGTAAT1660.10180364162665047No Hit
TCGCGCGGGCTCAAGACACATAATCATCAGATTCACCAAGGTTGAAATGAAG1660.10180364162665047No Hit
ACACAACAGTCTCCAGAGTGTGATGTTCCCCTTCCTGTGTCCATGTGTTCTC1660.10180364162665047No Hit
GTATCTGGGAACTTCATTGTCACTTCAAAACCCCCTCAGGTTAAATAGCTTC1650.10119036667709234No Hit
AAAATAGGGTCTGTCTGCGGATACATTATGGAGCTTGAGTTGGAAAACACCC1650.10119036667709234No Hit
CATCCCGGGTTACTGAGAATTTGGCTCCTACCTTTGAAGGATGGTCTTTTGA1650.10119036667709234No Hit
ATAGGGGAGCCCACCTCAGATTCTTAAATGATAGTTTGATGGGACAATCATG1650.10119036667709234No Hit
GATCGCGGGAGGCCATGTTTCTGATGTTATAGGCGAAGGAGATGCAGCAGAT1650.10119036667709234No Hit
ATTTCTGGGTTGGTATTGATGTGGGTCAGAAGGAGCCTCATCCTAATCTCAC1640.1005770917275342No Hit
TTATTAGGGACTCCTGTGCTCAAGCGATCCTCCTGCCTCGGCTTCTCAAAGT1640.1005770917275342No Hit
CGAACGACGGGAGGCCAGTTCACTGTTCCTCACAGCTTGGCTCTTGTGCCTC1640.1005770917275342No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)