FastQCFastQC Report
Sat 31 Dec 2022
SRR5847941.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847941.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160943
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGC6310.3920642712016056No Hit
CTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGCCGTCT6050.3759094834817296TruSeq Adapter, Index 3 (95% over 22bp)
GTAATGGGAATTTGTTCATGGTTATTCTACATTTCTAGAAAGTTCTTATCAG2010.12488893583442585No Hit
TTTCTGGGGACTTGCCGCAGTACTCTTAAAACTAGGCGGCTATGGTATAATA1950.12116090789906986No Hit
GCTAATGGGAGTTTTTCTTAAGCTTTTGGACTGGTAAGTGGCATTTATGAAA1930.11991823192061786No Hit
TTTGCGTGGGGAGCTACAGACATCTCTGCATATAGTTAACAGTTTTAACATA1920.11929689393139185No Hit
TTCTCTGGGGGGAACGGGAGAGAGAAAGAGAAAAAGAGAGAGATCGTGAAAG1900.11805421795293987No Hit
GACAGGGGAGGGAGTCGAGGGCAGAGGCAGCAGGCGGAGAGAGGGAGATAGG1890.11743287996371388No Hit
TAGCAAAGGGAATATACAGAACTCCAAAATAGCTTCATTTAAGGATTTTTTT1880.11681154197448786No Hit
TTAAAAGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGCG1860.11556886599603587No Hit
TACGCTGGGGTGACTTAATGTTTGATTAGGAAATATTTTGTTTGGTGTAAGG1850.11494752800680985No Hit
TGGCCTGGGAGTGGAGCCTCCTAGTGGCCGTGGTTGCAGGCTCTGTGGTCAG1830.11370485202835787No Hit
TGCTTTGGGCAAGTCAATGACCAGATTGTGGAAGTGGATGGAATCAGCTTGG1820.11308351403913187No Hit
ACTCTCGGGCAACCAAAATATCTACAGTAGGTGAATGGTTAAAGAAACTGTG1820.11308351403913187No Hit
AATCTGGGGAACATTAATAATGCCAGGGCTTTTAGGCAAAGGATGTAATTCA1810.11246217604990585No Hit
TAGTTAGGGCTTACAGATGTCAGGTAATTTGTTTCAAAAATTTGTGAGTTCA1790.11121950007145387No Hit
TTGTCGGGGAGACAGAAAGTTAGCAAGGATATCCAGGAATTGAACTCAGCTC1790.11121950007145387No Hit
AAGAAGGGGAAGCACTGAGATTGCAGGCATGAGTCACTGTGCCCAGTATTTT1790.11121950007145387No Hit
TACGCAGGGTGAATATTATTGATGACTAGACACCTGCTAGGATGTAAAGGCA1790.11121950007145387No Hit
GTGCTGAGGGGCCCTTCCTCCCTCCCTCACACGCCTCCACCTCTTCCCGATC1790.11121950007145387No Hit
TGAAGTGGGGATAAGGCCACGTTTCATCTCCTGTTACAATTCTTCAAAGAAA1780.11059816208222786No Hit
AACCAGGGGGGACTTTTGACAAACAGATCGTCTTCGTTCTGAATCCTCAGTG1780.11059816208222786No Hit
TTAATGGGGAAAAACACAATACCAGGCATACATTTGGCAGAATAAATGAACC1770.10997682409300187No Hit
GTATCTGGGAACTTCATTGTCACTTCAAAACCCCCTCAGGTTAAATAGCTTC1760.10935548610377588No Hit
ATTGTGGGGATAGGCTTAAGTCTATCATTTGCTCTTTTGTTTTATTGTCTGT1760.10935548610377588No Hit
AAAGCAGGGAACACTAAGTCTGCTGGTGTCTTGATCTTGGGATTCCCAGCCT1760.10935548610377588No Hit
TGTAATGGGATAGTAATACATCTAACCAAAGAGGAGAAAGATCTCTACAAGG1760.10935548610377588No Hit
TAACACAGGGAGGAACTGTAAGCTTTTATCTTTTAACCAACTGAACAATACA1760.10935548610377588No Hit
GGCTTAGGGTGTGATAAAATCAGGCTAATTTACTTGGATTTGGGGTGATTTG1750.10873414811454987No Hit
ACTTACGGGACCACCAGCCCACCAGATAAAGGCCCCCCAAAGAGTAAACTAA1740.10811281012532387No Hit
ATTGGTGGGTGTTGTCTTGTGTACCTGTTGGGCTGCTAATTCATTCTGCTCT1740.10811281012532387No Hit
TTATTAGGGACTCCTGTGCTCAAGCGATCCTCCTGCCTCGGCTTCTCAAAGT1740.10811281012532387No Hit
ATTTTAGGGGAGTGCAAATATCCAAGAAGAGGAAGTTTGTCGCTGATGGCAT1740.10811281012532387No Hit
AGAAGTGGGCGATCTTATATCGGGGATCTTCTTAAGGGAGATATAAATAGAA1730.10749147213609787No Hit
GACCCTCCTGAAGACTGTCTCATCGCAAGCCACTGGTGGCTGAACACAGCAC1720.10687013414687188No Hit
CTTTGTGGGAAGATTTTGCCAAGTATGTGTTGTTGCTTGACTTCTTAAATAT1720.10687013414687188No Hit
TAGTCGGGGCACTGTAATTGTGATTTGCAATCCACTTATAACTCTAGTGTGA1710.10624879615764589No Hit
TGGCTCACTGCAGCCTCCGCCTCCTGGGTTCAGGTGATTCTCCTGCCTCAGC1700.10562745816841987No Hit
CATACTGGGACTAGGTGTTACAGCAATATTTTAAGAGCTTGCTCTTTTATTT1690.10500612017919388No Hit
ATAACTGGGAAACAAGGCATGCCACACCATTGCTGTTCCCCATCTGGTACTA1690.10500612017919388No Hit
AGGCGGGAGGTCAAAAAACAAAGGGCAAAGCAGACATTTTAGTATACAGAGT1680.10438478218996788No Hit
CAAGAGGGGAATAATGTTTATTATGTTTGGAAAATTACATTAAATGCATTAA1680.10438478218996788No Hit
TCTTTGGGGGAATAAGAGCCTTGATGCAAAAGAACCACCGTCTGACTTGACA1680.10438478218996788No Hit
TGAAATGGGTCCCCCTCACAGATCTCTGCGGCTGCTGATTGGTAAAGGAAGC1680.10438478218996788No Hit
TAGTTTGGGGCTCGTGGGCTGGTGACTGGGTCTTGGGATCCCAGGCCACGCG1680.10438478218996788No Hit
CAACCTGGGAAACTTCACTGAGAGCACAGATGGAGGAAAGGCCATTTTAAAA1670.10376344420074186No Hit
ATTAGGGGGTTGAAAAGAAGTCCAGAATATTCCCTGGTGATACAATTCTGGA1670.10376344420074186No Hit
CTGTTTGGGATTGCTTGAGCACAGGAGGTTGAGACTGCAGTGAGTCAAGATC1670.10376344420074186No Hit
TGCCATAGGGTAGCAGAGATCACCAATGCTTGCTTTGAGCCAGCCAACCAGA1660.10314210621151587No Hit
TGTAACGGGTTATTGGATTTGCACCTAACATAGATCATAATTTTTGTATCTT1650.10252076822228988No Hit
TGGCGGGTCTTATGTTTGTGACTATAGACACAAACATCCTCAATAAGGTATT1650.10252076822228988No Hit
CTAGGAGGGGCCAGGAATGTGCCCCTTCTCTAAGAAAATGCCCTATTTGCAG1650.10252076822228988No Hit
TGCTCCGGGTCCTTAACTACATATGCTAAGCCCTTTCGTGCTTCCAGCTTAA1640.10189943023306389No Hit
GGCATGGATGCATAAAAGTGTTTCAAAAACTTCCTAGCACAAAGATAGTAAT1640.10189943023306389No Hit
AAGCTTGGGCATCAGCATTTTCTTGCAACTTTAAAAATCACAAGGGATTATT1640.10189943023306389No Hit
AGGAATGGGAGAGGAGTCATGGATCCACTGGACAAGGTTCTGTCAGTTCTTA1640.10189943023306389No Hit
TACATAGGGTGATCAATCTAACCTAATTTTGACTTGTTAATAAAGAAAGGTA1640.10189943023306389No Hit
GCTAAGGGCATATATTGCCCTGAAACCATCTGGTTTAACTTGTCCCTCAATC1640.10189943023306389No Hit
CTACTGGGGAGGCGGCGGCGCAGGCGGCGGCGGCGGCATAGCGCACAGCGCG1630.10127809224383788No Hit
GATAGAGGGTGCGAGGGGAACTGGAACAAACAAGATTAGAAGCTGAATTTTC1630.10127809224383788No Hit
TGTGTGGGGATAGATAACTTCCAAAGTTCTTTTGCCTCTGAATTTCTATGAC1620.10065675425461187No Hit
GCCCTGGGCCACCTACTATATTAATCATGCTAGTAACATGAAAAATGATGGG1620.10065675425461187No Hit
CATCCCGGGTTACTGAGAATTTGGCTCCTACCTTTGAAGGATGGTCTTTTGA1620.10065675425461187No Hit
ATTGCAGGGGTACATAAAATATTTAAATCAACTTAATCTATGTACATGTTAT1620.10065675425461187No Hit
ACACAACAGTCTCCAGAGTGTGATGTTCCCCTTCCTGTGTCCATGTGTTCTC1620.10065675425461187No Hit
TCTCAGGGGATCTCGGCTCACTGCAAGCTCTGCCTCCTAGGTTCATGCCATT1620.10065675425461187No Hit
GTCAAAGGATTTCTTATTTCTTAAAGATCCTATATATGAAATAAACAATATG1610.10003541626538588No Hit
TTGGCTGAGCTGAGACACCTCCTGTTTCCCTTCCCCTTGAACCAGTCCCAGT1610.10003541626538588No Hit
ATTCCGGGACATCAAAGGTGCTCTTATTGCCTCCAGATGTTTCTGCTTTGGC1610.10003541626538588No Hit
GAGCAAGGGAACATACATTTTGAAATTATGAACATTTTTTTCATAAAAATTA1610.10003541626538588No Hit
CATCACGGGGATGTATGTGTTAATTTAATTTGTTAAAAACCAAAAAGCAAAA1610.10003541626538588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)