FastQCFastQC Report
Sat 31 Dec 2022
SRR5847919.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847919.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137191
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGCCGTCT3550.2587633299560467TruSeq Adapter, Index 3 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATTATGCATCTCGTATGC3000.21867323658257468No Hit
CCAGCCTGGGTGACAGAGACCCTGTGTCAAACAAAACAAAACAAAAACAAAA1560.11371008302293883No Hit
ACACTAGGGATTCAAATTCAGGAAATACAGACAACACCACAAAGTTACTTCT1530.11152335065711308No Hit
GAGGCCGAGGCAAGAGAATCGCTTGAACCCGGGAGGCGGAGGATGCAGAGAG1510.11006552907989592No Hit
AGCTAAGGGATTGATTTGTGTATGTTGAACCAGGCTTGCATCCCAGGGATGA1500.10933661829128734No Hit
CCACTGGAATATTCTAGAATAAGAGCACTGGATAAGTACACTTGAACACATT1460.10642097513685302No Hit
ATTAAAGGGTGGAAAGGTAGTGATTCTCAAAAGTTATTCTCAGTCTTCCTTC1460.10642097513685302No Hit
TCTGCGAGGGTCACAACAAACCTGTGAAACTAGTGCTTGCATTTCTGCTCTA1430.10423424277102725No Hit
CAACTAGGGTCTTTCGCAGGGATCTGCTCATAGAGTAAAATAAAACATGACT1430.10423424277102725No Hit
AGGCATGGGTCTCTGGCGGGGACTCAAGTGGACGGGGTGACTCCTTTGCATG1410.1027764211938101No Hit
ACATCTGGGGCGGAGCTTCATGTCTTATGTAACCATAGTATAATTATCAAAA1400.10204751040520152No Hit
TTCTGGGATAACTGGTGTTTTATTCACAAAAGTCTCCTTCCTTTAAGACATC1400.10204751040520152No Hit
TGAATAGGGGGCTGAACACAGACAATTTAAAAAAACATGTGGTCAAAGGACC1390.10131859961659292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)